Mucilaginibacter sp. HME9299

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4IC75|A0A6I4IC75_9SPHI LacI family DNA-binding transcriptional regulator OS=Mucilaginibacter sp. HME9299 OX=1517760 GN=GO816_16975 PE=4 SV=1
MM1 pKa = 6.76EE2 pKa = 4.05QAVFLLATDD11 pKa = 4.13PNEE14 pKa = 3.87PCRR17 pKa = 11.84DD18 pKa = 3.73VIHH21 pKa = 7.24SRR23 pKa = 11.84DD24 pKa = 3.07TDD26 pKa = 3.84LKK28 pKa = 9.51VKK30 pKa = 9.39VYY32 pKa = 10.6CVTDD36 pKa = 3.62EE37 pKa = 4.59SFTPDD42 pKa = 2.79TDD44 pKa = 4.21EE45 pKa = 4.24IQLYY49 pKa = 10.56AYY51 pKa = 10.27ANNKK55 pKa = 9.57LYY57 pKa = 10.8AFEE60 pKa = 5.03TINIAPDD67 pKa = 3.58DD68 pKa = 4.28ALDD71 pKa = 3.72VVSAIQWYY79 pKa = 9.86ASYY82 pKa = 11.01IEE84 pKa = 4.14FPEE87 pKa = 4.07MEE89 pKa = 5.12ILPEE93 pKa = 4.73DD94 pKa = 3.83PRR96 pKa = 11.84LDD98 pKa = 3.25QDD100 pKa = 3.55IAII103 pKa = 4.69

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4IDY4|A0A6I4IDY4_9SPHI Tetratricopeptide repeat protein OS=Mucilaginibacter sp. HME9299 OX=1517760 GN=GO816_11820 PE=3 SV=1
MM1 pKa = 7.24KK2 pKa = 10.12RR3 pKa = 11.84SFIIDD8 pKa = 3.51MVISLLVLLFVHH20 pKa = 6.12TAVSKK25 pKa = 10.43LLAYY29 pKa = 10.28ADD31 pKa = 4.04YY32 pKa = 9.33RR33 pKa = 11.84LSMGRR38 pKa = 11.84QALPKK43 pKa = 10.13ALLPVLVPVLPAAEE57 pKa = 4.31LLAAACLLFRR67 pKa = 11.84RR68 pKa = 11.84TRR70 pKa = 11.84RR71 pKa = 11.84IGLYY75 pKa = 10.01VSAILMLVFTTYY87 pKa = 10.49VALAVSGFFPHH98 pKa = 7.15LPCSCGGVIAGMGWTAHH115 pKa = 7.17LILNVIVLLLNILAIVLEE133 pKa = 3.99NRR135 pKa = 11.84EE136 pKa = 3.65RR137 pKa = 11.84RR138 pKa = 11.84IRR140 pKa = 11.84LSHH143 pKa = 6.51RR144 pKa = 3.39

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3655

0

3655

1293125

26

2327

353.8

39.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.956 ± 0.037

0.771 ± 0.014

5.296 ± 0.03

5.428 ± 0.041

4.714 ± 0.028

6.827 ± 0.038

1.897 ± 0.019

6.796 ± 0.033

6.778 ± 0.037

9.435 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.015

5.85 ± 0.037

3.839 ± 0.024

4.006 ± 0.027

4.122 ± 0.025

6.137 ± 0.032

5.856 ± 0.034

6.659 ± 0.026

1.207 ± 0.019

4.172 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski