Acinetobacter phage vB_AbaM_IME512
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G3BXR7|A0A3G3BXR7_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME512 OX=2419627 PE=4 SV=1
MM1 pKa = 7.25 AQYY4 pKa = 9.85 DD5 pKa = 3.73 IPLAQVPNQFFTTSLNGVTWEE26 pKa = 3.92 ITLEE30 pKa = 3.99 TRR32 pKa = 11.84 LNNLYY37 pKa = 10.27 ISLSNNNDD45 pKa = 2.72 GDD47 pKa = 3.93 VLLNRR52 pKa = 11.84 ICLNRR57 pKa = 11.84 TYY59 pKa = 10.23 LGHH62 pKa = 6.75 GFIFVDD68 pKa = 3.51 IDD70 pKa = 3.79 GNSDD74 pKa = 3.53 PEE76 pKa = 4.25 YY77 pKa = 9.62 TGLGTRR83 pKa = 11.84 YY84 pKa = 10.14 LLIWTDD90 pKa = 3.71 EE91 pKa = 4.13 VV92 pKa = 3.51
Molecular weight: 10.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.849
IPC2_protein 4.101
IPC_protein 3.986
Toseland 3.783
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.935
Rodwell 3.821
Grimsley 3.706
Solomon 3.961
Lehninger 3.923
Nozaki 4.113
DTASelect 4.329
Thurlkill 3.859
EMBOSS 3.948
Sillero 4.113
Patrickios 0.782
IPC_peptide 3.961
IPC2_peptide 4.088
IPC2.peptide.svr19 3.983
Protein with the highest isoelectric point:
>tr|A0A3G3BXV7|A0A3G3BXV7_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME512 OX=2419627 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.39 LKK4 pKa = 10.51 RR5 pKa = 11.84 KK6 pKa = 8.17 VRR8 pKa = 11.84 RR9 pKa = 11.84 GEE11 pKa = 3.88 MTKK14 pKa = 10.16 TSIDD18 pKa = 3.55 QEE20 pKa = 3.77 IDD22 pKa = 2.99 EE23 pKa = 5.74 AIAKK27 pKa = 9.91 GGSFYY32 pKa = 10.86 KK33 pKa = 10.0 IRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 FEE39 pKa = 3.54 KK40 pKa = 10.65 AILEE44 pKa = 4.06 RR45 pKa = 11.84 ALIKK49 pKa = 10.08 TRR51 pKa = 11.84 GNQTEE56 pKa = 4.03 AAKK59 pKa = 9.89 MLGISRR65 pKa = 11.84 SGLGGILKK73 pKa = 10.32 RR74 pKa = 11.84 VGRR77 pKa = 4.23
Molecular weight: 8.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 9.926
IPC_protein 11.038
Toseland 11.374
ProMoST 11.711
Dawson 11.389
Bjellqvist 11.228
Wikipedia 11.725
Rodwell 11.55
Grimsley 11.418
Solomon 11.725
Lehninger 11.652
Nozaki 11.359
DTASelect 11.228
Thurlkill 11.359
EMBOSS 11.813
Sillero 11.359
Patrickios 11.286
IPC_peptide 11.74
IPC2_peptide 10.526
IPC2.peptide.svr19 9.076
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
91
0
91
14130
36
676
155.3
17.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.759 ± 0.341
1.217 ± 0.149
6.136 ± 0.238
6.709 ± 0.361
4.459 ± 0.192
6.999 ± 0.265
1.734 ± 0.149
7.247 ± 0.234
7.806 ± 0.377
8.082 ± 0.229
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.845 ± 0.164
6.044 ± 0.239
2.979 ± 0.17
3.758 ± 0.223
3.935 ± 0.174
6.086 ± 0.203
5.357 ± 0.333
6.355 ± 0.241
1.479 ± 0.114
4.013 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here