Melon partitivirus
Average proteome isoelectric point is 5.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346RRG4|A0A346RRG4_9VIRU Putative coat protein OS=Melon partitivirus OX=2305257 GN=CP PE=4 SV=1
MM1 pKa = 7.58 AALGDD6 pKa = 4.39 GVPPIADD13 pKa = 3.35 NNNPQGRR20 pKa = 11.84 DD21 pKa = 3.25 ANPIRR26 pKa = 11.84 GGTDD30 pKa = 3.19 PLRR33 pKa = 11.84 RR34 pKa = 11.84 ATDD37 pKa = 3.27 WLEE40 pKa = 4.42 DD41 pKa = 3.47 NSKK44 pKa = 8.63 ITMTFHH50 pKa = 6.76 RR51 pKa = 11.84 HH52 pKa = 3.67 ANYY55 pKa = 9.86 RR56 pKa = 11.84 RR57 pKa = 11.84 YY58 pKa = 9.1 ATLRR62 pKa = 11.84 LQEE65 pKa = 4.2 MFNALVQLYY74 pKa = 9.5 QNIFMSHH81 pKa = 4.26 WHH83 pKa = 5.84 HH84 pKa = 7.02 LRR86 pKa = 11.84 RR87 pKa = 11.84 TVEE90 pKa = 4.09 NYY92 pKa = 8.94 QVPAPLAQTPWLYY105 pKa = 10.66 VARR108 pKa = 11.84 LYY110 pKa = 10.67 ISVWIVDD117 pKa = 4.38 LYY119 pKa = 11.28 ASIRR123 pKa = 11.84 RR124 pKa = 11.84 ACLRR128 pKa = 11.84 VCPEE132 pKa = 4.62 AYY134 pKa = 10.08 NEE136 pKa = 4.77 YY137 pKa = 9.11 YY138 pKa = 8.62 TQDD141 pKa = 3.35 YY142 pKa = 8.56 QQVVFEE148 pKa = 4.31 YY149 pKa = 11.01 DD150 pKa = 3.0 AFLSQLLSMIKK161 pKa = 8.46 PTVVNLVHH169 pKa = 6.21 EE170 pKa = 4.66 TTLFVPRR177 pKa = 11.84 LAEE180 pKa = 4.29 DD181 pKa = 3.65 QPFPADD187 pKa = 3.57 DD188 pKa = 3.7 MNIFGLDD195 pKa = 2.81 RR196 pKa = 11.84 FDD198 pKa = 4.15 YY199 pKa = 11.0 NEE201 pKa = 4.03 NVVHH205 pKa = 6.37 GVISILRR212 pKa = 11.84 DD213 pKa = 3.21 RR214 pKa = 11.84 KK215 pKa = 10.4 LMKK218 pKa = 10.17 FEE220 pKa = 4.84 KK221 pKa = 10.03 PSEE224 pKa = 4.27 TGFGRR229 pKa = 11.84 PFWLFDD235 pKa = 2.98 WHH237 pKa = 6.15 RR238 pKa = 11.84 TGHH241 pKa = 6.24 AYY243 pKa = 10.23 AWFPAEE249 pKa = 4.07 NNYY252 pKa = 10.82 DD253 pKa = 3.59 MRR255 pKa = 11.84 DD256 pKa = 3.3 VTVAYY261 pKa = 9.37 IVGVACTPKK270 pKa = 10.53 LGPRR274 pKa = 11.84 DD275 pKa = 3.4 IDD277 pKa = 3.55 DD278 pKa = 3.7 WQHH281 pKa = 5.74 FPGNIIPEE289 pKa = 4.09 HH290 pKa = 6.55 PRR292 pKa = 11.84 AEE294 pKa = 3.94 DD295 pKa = 3.34 HH296 pKa = 6.61 VRR298 pKa = 11.84 VRR300 pKa = 11.84 PVAYY304 pKa = 9.75 HH305 pKa = 5.88 GAVEE309 pKa = 4.11 VRR311 pKa = 11.84 TIDD314 pKa = 3.62 VQQWTAPPEE323 pKa = 4.25 VMSLIQVTLPPAAAQQQQQQQPAAQAQPAPARR355 pKa = 11.84 QQPPAQQQQQEE366 pKa = 4.23 QGLIRR371 pKa = 11.84 YY372 pKa = 7.35 NLRR375 pKa = 11.84 QGFKK379 pKa = 10.33 RR380 pKa = 11.84 RR381 pKa = 11.84 RR382 pKa = 11.84 TDD384 pKa = 3.04 PSTSNDD390 pKa = 2.52 NDD392 pKa = 3.66 MPVEE396 pKa = 4.79 DD397 pKa = 4.77 IEE399 pKa = 5.26 EE400 pKa = 4.53 PPQPEE405 pKa = 4.01 QGEE408 pKa = 4.47 DD409 pKa = 3.67 PIHH412 pKa = 6.62 PEE414 pKa = 3.86 EE415 pKa = 4.34 EE416 pKa = 3.79 AAPPVEE422 pKa = 4.51 PQPDD426 pKa = 4.06 PAPAPAPAPQAPAEE440 pKa = 4.59 PIITMNRR447 pKa = 11.84 VRR449 pKa = 11.84 RR450 pKa = 11.84 FRR452 pKa = 11.84 IITYY456 pKa = 9.39 CYY458 pKa = 9.13 YY459 pKa = 10.71 RR460 pKa = 11.84 QVTDD464 pKa = 4.34 NEE466 pKa = 4.45 DD467 pKa = 3.17 LHH469 pKa = 8.3 SRR471 pKa = 11.84 LAALRR476 pKa = 11.84 QVDD479 pKa = 3.92 SLIAA483 pKa = 4.04
Molecular weight: 55.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.36
IPC2_protein 5.321
IPC_protein 5.334
Toseland 5.614
ProMoST 5.601
Dawson 5.486
Bjellqvist 5.55
Wikipedia 5.448
Rodwell 5.436
Grimsley 5.715
Solomon 5.486
Lehninger 5.461
Nozaki 5.69
DTASelect 5.881
Thurlkill 5.753
EMBOSS 5.715
Sillero 5.779
Patrickios 3.948
IPC_peptide 5.499
IPC2_peptide 5.792
IPC2.peptide.svr19 5.725
Protein with the highest isoelectric point:
>tr|A0A346RRG4|A0A346RRG4_9VIRU Putative coat protein OS=Melon partitivirus OX=2305257 GN=CP PE=4 SV=1
MM1 pKa = 7.3 LRR3 pKa = 11.84 DD4 pKa = 3.32 IRR6 pKa = 11.84 NFQLTEE12 pKa = 4.09 FNSEE16 pKa = 4.17 FEE18 pKa = 4.73 LLNDD22 pKa = 3.68 THH24 pKa = 6.32 PHH26 pKa = 5.6 VIRR29 pKa = 11.84 RR30 pKa = 11.84 EE31 pKa = 4.03 AEE33 pKa = 3.85 QILVDD38 pKa = 4.1 EE39 pKa = 5.0 FALSEE44 pKa = 4.8 LNGAWPSLYY53 pKa = 10.09 DD54 pKa = 3.51 QKK56 pKa = 11.5 LKK58 pKa = 10.36 GWARR62 pKa = 11.84 SFYY65 pKa = 9.74 TLEE68 pKa = 3.64 GHH70 pKa = 5.79 MQAIHH75 pKa = 7.02 AYY77 pKa = 9.37 SNPDD81 pKa = 3.38 TPISSLNEE89 pKa = 3.69 TIYY92 pKa = 10.8 RR93 pKa = 11.84 ATIDD97 pKa = 3.43 SLKK100 pKa = 10.94 NEE102 pKa = 4.31 LSSLPTARR110 pKa = 11.84 AFDD113 pKa = 3.8 VLTEE117 pKa = 3.99 LDD119 pKa = 4.08 KK120 pKa = 11.15 IHH122 pKa = 6.54 YY123 pKa = 6.93 EE124 pKa = 3.87 QSSAAGYY131 pKa = 10.74 NYY133 pKa = 9.8 IGPKK137 pKa = 9.72 GPLYY141 pKa = 10.92 GEE143 pKa = 3.8 NHH145 pKa = 5.44 EE146 pKa = 4.23 RR147 pKa = 11.84 AIRR150 pKa = 11.84 SARR153 pKa = 11.84 AVLWSAIRR161 pKa = 11.84 DD162 pKa = 3.77 EE163 pKa = 5.27 DD164 pKa = 3.76 GGPAYY169 pKa = 10.4 VLRR172 pKa = 11.84 NMVPDD177 pKa = 3.51 VGYY180 pKa = 8.76 TRR182 pKa = 11.84 TQLTNLQEE190 pKa = 4.12 KK191 pKa = 8.94 TKK193 pKa = 10.58 VRR195 pKa = 11.84 GVWGRR200 pKa = 11.84 AFHH203 pKa = 6.66 YY204 pKa = 10.28 ILLEE208 pKa = 4.32 GSSARR213 pKa = 11.84 PLLEE217 pKa = 4.37 RR218 pKa = 11.84 FLEE221 pKa = 4.51 HH222 pKa = 6.64 DD223 pKa = 3.56 TFYY226 pKa = 10.99 HH227 pKa = 6.82 IGEE230 pKa = 4.65 DD231 pKa = 3.71 SQLSVPKK238 pKa = 10.54 LMSKK242 pKa = 10.59 LSSEE246 pKa = 4.45 CRR248 pKa = 11.84 WIYY251 pKa = 10.84 AIDD254 pKa = 3.54 WSAFDD259 pKa = 3.51 ATVSRR264 pKa = 11.84 FEE266 pKa = 3.87 IEE268 pKa = 3.75 AAFEE272 pKa = 3.98 IMKK275 pKa = 10.64 GLIHH279 pKa = 6.79 FPNFDD284 pKa = 3.53 SEE286 pKa = 4.65 QAFEE290 pKa = 4.2 LSRR293 pKa = 11.84 LLFTHH298 pKa = 6.76 KK299 pKa = 10.38 KK300 pKa = 8.26 IAAPDD305 pKa = 3.27 GHH307 pKa = 6.56 TYY309 pKa = 9.86 WIHH312 pKa = 6.71 KK313 pKa = 7.85 GIPSGSYY320 pKa = 6.7 FTSMAGSIINRR331 pKa = 11.84 LRR333 pKa = 11.84 IGYY336 pKa = 8.8 LWRR339 pKa = 11.84 LMFNRR344 pKa = 11.84 EE345 pKa = 3.67 PKK347 pKa = 10.31 LCYY350 pKa = 9.16 TQGDD354 pKa = 3.91 DD355 pKa = 5.12 SLIGDD360 pKa = 4.86 DD361 pKa = 5.46 EE362 pKa = 5.31 LIQPEE367 pKa = 4.37 RR368 pKa = 11.84 LAEE371 pKa = 3.93 LAAPLNWNINPNKK384 pKa = 9.27 TEE386 pKa = 3.87 YY387 pKa = 10.84 SRR389 pKa = 11.84 TPEE392 pKa = 4.34 FISFLGRR399 pKa = 11.84 TSRR402 pKa = 11.84 GGFNTRR408 pKa = 11.84 EE409 pKa = 3.96 LEE411 pKa = 3.86 KK412 pKa = 10.69 CLRR415 pKa = 11.84 LLIFPEE421 pKa = 4.32 HH422 pKa = 6.71 PVPSGRR428 pKa = 11.84 ISAYY432 pKa = 8.99 RR433 pKa = 11.84 ARR435 pKa = 11.84 SINEE439 pKa = 3.68 DD440 pKa = 3.39 SGFTSDD446 pKa = 4.99 YY447 pKa = 8.73 IQTVARR453 pKa = 11.84 RR454 pKa = 11.84 LRR456 pKa = 11.84 RR457 pKa = 11.84 RR458 pKa = 11.84 YY459 pKa = 9.99 GIAEE463 pKa = 3.98 EE464 pKa = 4.61 SEE466 pKa = 4.24 VPKK469 pKa = 10.19 MFKK472 pKa = 10.21 RR473 pKa = 11.84 YY474 pKa = 7.63 VVRR477 pKa = 5.09
Molecular weight: 54.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.129
IPC2_protein 6.046
IPC_protein 6.148
Toseland 6.605
ProMoST 6.532
Dawson 6.44
Bjellqvist 6.427
Wikipedia 6.465
Rodwell 6.427
Grimsley 6.795
Solomon 6.44
Lehninger 6.44
Nozaki 6.707
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.825
Patrickios 4.266
IPC_peptide 6.465
IPC2_peptide 6.751
IPC2.peptide.svr19 6.723
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
960
477
483
480.0
55.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.333 ± 0.789
0.729 ± 0.066
5.729 ± 0.594
6.979 ± 0.784
4.271 ± 0.498
4.688 ± 0.774
3.021 ± 0.056
6.042 ± 0.574
2.604 ± 0.766
8.333 ± 1.133
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.979 ± 0.198
4.375 ± 0.119
7.5 ± 1.754
5.313 ± 1.831
8.229 ± 0.103
5.417 ± 1.807
4.896 ± 0.089
5.208 ± 1.214
1.875 ± 0.008
4.479 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here