Mycoplasma neophronis
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 623 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A505IGD1|A0A505IGD1_9MOLU Site-specific integrase OS=Mycoplasma neophronis OX=872983 GN=FJR74_01605 PE=4 SV=1
AA1 pKa = 8.04 RR2 pKa = 11.84 DD3 pKa = 3.35 VGGPPLVFQLLWYY16 pKa = 6.36 PTTMADD22 pKa = 3.91 LSLPSFTEE30 pKa = 3.86 NADD33 pKa = 3.52 APILDD38 pKa = 4.34 RR39 pKa = 11.84 DD40 pKa = 4.04 VIDD43 pKa = 5.95 AFLAWYY49 pKa = 10.27 VPGLDD54 pKa = 4.04 ISDD57 pKa = 3.54 HH58 pKa = 5.72 TMLPTTLAPGNADD71 pKa = 3.98 LSGLPPAFIGTAEE84 pKa = 4.42 HH85 pKa = 7.61 DD86 pKa = 3.94 PLRR89 pKa = 11.84 DD90 pKa = 3.76 DD91 pKa = 4.27 GACYY95 pKa = 10.72 AEE97 pKa = 5.13 LLTAAGVSVEE107 pKa = 4.41 LSNEE111 pKa = 3.55 PTMVHH116 pKa = 6.51 GYY118 pKa = 10.77 VNFALVVPAAAEE130 pKa = 3.87 ATGRR134 pKa = 11.84 GLAALKK140 pKa = 10.38 RR141 pKa = 11.84 ALHH144 pKa = 6.0 AA145 pKa = 4.96
Molecular weight: 15.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.173
IPC2_protein 4.202
IPC_protein 4.139
Toseland 3.948
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.075
Rodwell 3.973
Grimsley 3.859
Solomon 4.126
Lehninger 4.088
Nozaki 4.253
DTASelect 4.495
Thurlkill 3.999
EMBOSS 4.088
Sillero 4.266
Patrickios 3.274
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.165
Protein with the highest isoelectric point:
>tr|A0A505IMN9|A0A505IMN9_9MOLU Protein translocase subunit SecY OS=Mycoplasma neophronis OX=872983 GN=secY PE=3 SV=1
MM1 pKa = 7.73 ADD3 pKa = 3.69 KK4 pKa = 10.46 IRR6 pKa = 11.84 YY7 pKa = 8.14 YY8 pKa = 11.18 GLGRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 10.01 SSVARR19 pKa = 11.84 VYY21 pKa = 10.91 LLPGKK26 pKa = 10.75 GNFIINGKK34 pKa = 6.91 PAKK37 pKa = 9.62 EE38 pKa = 4.14 HH39 pKa = 6.4 LNSDD43 pKa = 3.69 ILLKK47 pKa = 10.81 DD48 pKa = 3.32 ALSPFTVTEE57 pKa = 4.03 TTNQFDD63 pKa = 3.3 VFANVNGGGLTGQAGAIRR81 pKa = 11.84 LGIARR86 pKa = 11.84 ALLEE90 pKa = 4.27 ASNNEE95 pKa = 3.78 YY96 pKa = 10.57 RR97 pKa = 11.84 NKK99 pKa = 10.73 LKK101 pKa = 10.82 DD102 pKa = 3.29 AGFLTRR108 pKa = 11.84 DD109 pKa = 3.24 ARR111 pKa = 11.84 VKK113 pKa = 9.75 EE114 pKa = 4.1 RR115 pKa = 11.84 KK116 pKa = 9.77 KK117 pKa = 10.69 FGLRR121 pKa = 11.84 KK122 pKa = 9.46 ARR124 pKa = 11.84 RR125 pKa = 11.84 ARR127 pKa = 11.84 QFSKK131 pKa = 10.87 RR132 pKa = 3.51
Molecular weight: 14.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.94
IPC_protein 10.818
Toseland 11.082
ProMoST 11.096
Dawson 11.14
Bjellqvist 10.862
Wikipedia 11.374
Rodwell 11.389
Grimsley 11.169
Solomon 11.33
Lehninger 11.286
Nozaki 11.052
DTASelect 10.862
Thurlkill 11.067
EMBOSS 11.491
Sillero 11.067
Patrickios 11.111
IPC_peptide 11.33
IPC2_peptide 9.706
IPC2.peptide.svr19 8.569
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
623
0
623
223019
30
2968
358.0
40.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.449 ± 0.136
0.552 ± 0.027
5.503 ± 0.077
7.031 ± 0.108
4.909 ± 0.083
4.834 ± 0.092
1.43 ± 0.036
9.078 ± 0.119
9.395 ± 0.097
9.383 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.966 ± 0.043
7.969 ± 0.178
2.941 ± 0.061
3.318 ± 0.056
3.084 ± 0.073
6.286 ± 0.075
5.054 ± 0.06
5.677 ± 0.077
0.912 ± 0.032
4.231 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here