Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Pedobacter; Pedobacter heparinus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6Y0A9|C6Y0A9_PEDHD Regulatory protein ArsR OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=Phep_2618 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.47DD21 pKa = 3.38EE22 pKa = 5.13LIDD25 pKa = 3.42YY26 pKa = 7.68GIRR29 pKa = 11.84SGAPVEE35 pKa = 4.53VIEE38 pKa = 4.56NLQALEE44 pKa = 4.69DD45 pKa = 4.28DD46 pKa = 4.28GEE48 pKa = 4.35PYY50 pKa = 9.33EE51 pKa = 4.57TIEE54 pKa = 4.84EE55 pKa = 4.08IWPDD59 pKa = 3.97YY60 pKa = 7.47PTKK63 pKa = 10.94DD64 pKa = 3.05DD65 pKa = 4.15FFFNEE70 pKa = 4.51DD71 pKa = 3.17EE72 pKa = 4.46YY73 pKa = 11.99

Molecular weight: 8.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6XU81|C6XU81_PEDHD Single-stranded-DNA-specific exonuclease RecJ OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=Phep_3683 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.3HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.54GRR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84LTVSSEE47 pKa = 3.76GRR49 pKa = 11.84HH50 pKa = 4.97KK51 pKa = 11.04AA52 pKa = 3.25

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4249

0

4249

1593950

31

4896

375.1

41.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.84 ± 0.041

0.759 ± 0.011

5.218 ± 0.022

5.549 ± 0.034

4.797 ± 0.031

7.087 ± 0.036

1.722 ± 0.018

7.067 ± 0.034

7.162 ± 0.038

9.597 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.019

5.691 ± 0.032

3.855 ± 0.023

3.602 ± 0.02

3.916 ± 0.027

6.274 ± 0.036

5.801 ± 0.055

6.34 ± 0.029

1.225 ± 0.013

4.194 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski