Xiphophorus maculatus (Southern platyfish) (Platypoecilus maculatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35279 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B5R7X3|A0A3B5R7X3_XIPMA AdoHcyase_NAD domain-containing protein OS=Xiphophorus maculatus OX=8083 PE=3 SV=1
MM1 pKa = 7.39YY2 pKa = 10.41LEE4 pKa = 4.79NSEE7 pKa = 4.01QGKK10 pKa = 10.25VGGEE14 pKa = 4.4GEE16 pKa = 4.24LSAGAILSGQCAVSKK31 pKa = 8.99VTGVAGLSSSSKK43 pKa = 10.59GSEE46 pKa = 3.88LDD48 pKa = 2.96IKK50 pKa = 11.21ALVGYY55 pKa = 7.3TEE57 pKa = 4.21QSVSGGMDD65 pKa = 3.68PPPLCNMNAPQTQLCNDD82 pKa = 3.86CEE84 pKa = 4.31GLTEE88 pKa = 4.16SFEE91 pKa = 4.71EE92 pKa = 4.36YY93 pKa = 10.4SSSNGLFEE101 pKa = 5.59PDD103 pKa = 3.18GALEE107 pKa = 4.12DD108 pKa = 4.49TEE110 pKa = 5.05CSGLSRR116 pKa = 11.84LYY118 pKa = 10.71HH119 pKa = 6.91EE120 pKa = 5.11CTQHH124 pKa = 6.63CEE126 pKa = 3.9CFKK129 pKa = 10.38ACKK132 pKa = 10.03SSEE135 pKa = 3.77HH136 pKa = 6.51CANHH140 pKa = 5.87SLASKK145 pKa = 10.76SSLCCEE151 pKa = 4.39HH152 pKa = 6.8CPKK155 pKa = 10.59HH156 pKa = 6.58LLLFQQCKK164 pKa = 9.97PSDD167 pKa = 3.8QQSDD171 pKa = 3.72SSDD174 pKa = 3.72YY175 pKa = 11.23EE176 pKa = 4.0PDD178 pKa = 3.42EE179 pKa = 5.43LEE181 pKa = 4.29VNSDD185 pKa = 3.4SFGEE189 pKa = 4.09FEE191 pKa = 4.25MPGFVSEE198 pKa = 4.29CTEE201 pKa = 3.89NLMLPLDD208 pKa = 4.15RR209 pKa = 11.84QCEE212 pKa = 4.23DD213 pKa = 3.42SDD215 pKa = 4.47SEE217 pKa = 4.17QDD219 pKa = 3.57RR220 pKa = 11.84STEE223 pKa = 3.75DD224 pKa = 3.09SEE226 pKa = 5.67QNFTQNISDD235 pKa = 3.82SMDD238 pKa = 3.55SLTCAANFSEE248 pKa = 4.41YY249 pKa = 10.82DD250 pKa = 3.46EE251 pKa = 5.39SQDD254 pKa = 3.53NYY256 pKa = 10.81CDD258 pKa = 3.94EE259 pKa = 4.53EE260 pKa = 4.58EE261 pKa = 4.09EE262 pKa = 4.9SYY264 pKa = 11.63DD265 pKa = 3.56HH266 pKa = 7.47NEE268 pKa = 3.96MVSGEE273 pKa = 4.1EE274 pKa = 4.06SSTLSEE280 pKa = 4.55DD281 pKa = 3.98TPGDD285 pKa = 3.51PCEE288 pKa = 4.49SDD290 pKa = 3.16GGEE293 pKa = 4.04EE294 pKa = 4.4NIEE297 pKa = 3.95QTSTGDD303 pKa = 3.03ASEE306 pKa = 5.3DD307 pKa = 3.21VDD309 pKa = 4.06EE310 pKa = 4.33VHH312 pKa = 7.45RR313 pKa = 11.84DD314 pKa = 3.5DD315 pKa = 6.41SEE317 pKa = 5.57DD318 pKa = 3.52EE319 pKa = 4.3SSQHH323 pKa = 6.73GNFEE327 pKa = 4.32SCAEE331 pKa = 4.18EE332 pKa = 4.99DD333 pKa = 4.77LSSDD337 pKa = 3.43DD338 pKa = 3.71SKK340 pKa = 11.92SFKK343 pKa = 10.11TCLDD347 pKa = 3.1NSFPSDD353 pKa = 3.56PCSDD357 pKa = 3.43SSEE360 pKa = 4.42EE361 pKa = 3.96SDD363 pKa = 4.94KK364 pKa = 11.61AALDD368 pKa = 3.86DD369 pKa = 4.32TSDD372 pKa = 4.98EE373 pKa = 4.14PTQWEE378 pKa = 4.39SFEE381 pKa = 4.82EE382 pKa = 3.99NDD384 pKa = 4.61AEE386 pKa = 4.22KK387 pKa = 10.7QPSVEE392 pKa = 4.06KK393 pKa = 10.89SSEE396 pKa = 4.01EE397 pKa = 3.76EE398 pKa = 4.1DD399 pKa = 3.44KK400 pKa = 11.45KK401 pKa = 10.56KK402 pKa = 9.45TSAVNTVIEE411 pKa = 4.88DD412 pKa = 3.71YY413 pKa = 11.25FDD415 pKa = 4.29LFDD418 pKa = 4.52RR419 pKa = 11.84AGCSGQAFAQKK430 pKa = 10.23RR431 pKa = 11.84NYY433 pKa = 9.5ISCFDD438 pKa = 3.83GGDD441 pKa = 3.75VHH443 pKa = 7.22HH444 pKa = 7.04HH445 pKa = 5.92LHH447 pKa = 6.9LEE449 pKa = 4.01QEE451 pKa = 4.23ALKK454 pKa = 10.8QSVTTAHH461 pKa = 6.79KK462 pKa = 10.56EE463 pKa = 3.75EE464 pKa = 4.15TKK466 pKa = 10.75EE467 pKa = 4.11VIEE470 pKa = 4.11EE471 pKa = 4.09QEE473 pKa = 4.14KK474 pKa = 10.72DD475 pKa = 3.52VHH477 pKa = 5.87TGEE480 pKa = 4.25VTEE483 pKa = 4.67TNSQEE488 pKa = 4.1KK489 pKa = 10.57DD490 pKa = 3.32EE491 pKa = 4.67HH492 pKa = 8.14CEE494 pKa = 3.83DD495 pKa = 5.18SEE497 pKa = 5.44EE498 pKa = 4.12EE499 pKa = 4.35DD500 pKa = 3.99LNQRR504 pKa = 11.84DD505 pKa = 3.6TGSYY509 pKa = 10.8DD510 pKa = 3.42SEE512 pKa = 3.9NWTLKK517 pKa = 10.65SEE519 pKa = 4.2SSSTKK524 pKa = 10.73GEE526 pKa = 4.12AEE528 pKa = 4.0DD529 pKa = 4.07NEE531 pKa = 4.5PFGLYY536 pKa = 9.63PEE538 pKa = 5.13YY539 pKa = 11.01YY540 pKa = 9.54QDD542 pKa = 5.49SEE544 pKa = 5.29DD545 pKa = 3.25EE546 pKa = 4.15MYY548 pKa = 11.45YY549 pKa = 10.83NDD551 pKa = 4.41EE552 pKa = 3.65AWGYY556 pKa = 10.67DD557 pKa = 3.46GQISDD562 pKa = 5.53DD563 pKa = 3.72YY564 pKa = 11.8DD565 pKa = 3.86EE566 pKa = 5.45EE567 pKa = 5.21PEE569 pKa = 4.39SYY571 pKa = 10.72LDD573 pKa = 3.57SFRR576 pKa = 11.84AEE578 pKa = 4.07STFAPCAQEE587 pKa = 4.11IFVEE591 pKa = 4.55GDD593 pKa = 3.12VCEE596 pKa = 5.88DD597 pKa = 3.24IDD599 pKa = 5.57SVGEE603 pKa = 4.52DD604 pKa = 2.91IDD606 pKa = 4.63LVGEE610 pKa = 4.46VKK612 pKa = 10.67SKK614 pKa = 11.16ALTEE618 pKa = 4.16TTLDD622 pKa = 3.78LKK624 pKa = 11.23DD625 pKa = 4.18NKK627 pKa = 10.75NSDD630 pKa = 3.57AEE632 pKa = 4.22LDD634 pKa = 3.76GFSAFSEE641 pKa = 4.27MEE643 pKa = 4.41PYY645 pKa = 9.67WGLTEE650 pKa = 4.02EE651 pKa = 4.36QTRR654 pKa = 11.84EE655 pKa = 4.1CFPSGVEE662 pKa = 4.77DD663 pKa = 4.16YY664 pKa = 10.65YY665 pKa = 11.5AYY667 pKa = 10.35QIRR670 pKa = 11.84SIQSSDD676 pKa = 3.04ARR678 pKa = 11.84PLSRR682 pKa = 11.84FILDD686 pKa = 3.49NRR688 pKa = 11.84SSDD691 pKa = 3.33GKK693 pKa = 10.84AVGDD697 pKa = 3.71QGPEE701 pKa = 3.89DD702 pKa = 4.0VEE704 pKa = 4.24EE705 pKa = 4.47SSALLGINVLEE716 pKa = 5.28LIRR719 pKa = 11.84KK720 pKa = 6.27EE721 pKa = 3.95QSLEE725 pKa = 4.08PEE727 pKa = 4.35NEE729 pKa = 3.89LQGISDD735 pKa = 4.72LSPPLGFIHH744 pKa = 6.78SVASQHH750 pKa = 5.95EE751 pKa = 4.41ATPSQDD757 pKa = 3.55SEE759 pKa = 4.57EE760 pKa = 4.2EE761 pKa = 3.96QSDD764 pKa = 3.95DD765 pKa = 3.93EE766 pKa = 4.63SVEE769 pKa = 3.99NCEE772 pKa = 4.88CEE774 pKa = 4.22YY775 pKa = 10.7CIPPTQQVPVKK786 pKa = 9.76PLLSQSASDD795 pKa = 5.01DD796 pKa = 3.62PAKK799 pKa = 10.35ICVVIDD805 pKa = 3.67LDD807 pKa = 3.74EE808 pKa = 4.47TLVHH812 pKa = 7.13SSFKK816 pKa = 10.34PLNNADD822 pKa = 4.24FIIPVEE828 pKa = 3.94IEE830 pKa = 4.53GIVHH834 pKa = 6.02QVGTT838 pKa = 4.16

Molecular weight: 92.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B5QPY3|A0A3B5QPY3_XIPMA Uncharacterized protein OS=Xiphophorus maculatus OX=8083 GN=TENM3 PE=3 SV=1
MM1 pKa = 7.22VVPGLPRR8 pKa = 11.84RR9 pKa = 11.84LQVVPGLPRR18 pKa = 11.84HH19 pKa = 5.5LQVVPGLPRR28 pKa = 11.84RR29 pKa = 11.84FQVVPGLPRR38 pKa = 11.84RR39 pKa = 11.84LQVVPGLPRR48 pKa = 11.84RR49 pKa = 11.84FQVVPGLPRR58 pKa = 11.84RR59 pKa = 11.84LQVVPGLPRR68 pKa = 11.84RR69 pKa = 11.84LQVVPGLPRR78 pKa = 11.84RR79 pKa = 11.84LQVVPGLPRR88 pKa = 11.84RR89 pKa = 11.84LQVVPGLPRR98 pKa = 11.84RR99 pKa = 11.84LQVVPGLPRR108 pKa = 11.84RR109 pKa = 11.84LQVVPGLPRR118 pKa = 11.84RR119 pKa = 11.84FQVVPGLPRR128 pKa = 11.84RR129 pKa = 11.84LQVVPGLPRR138 pKa = 11.84RR139 pKa = 11.84LQVVPGLPRR148 pKa = 11.84RR149 pKa = 11.84LQVVPGLPRR158 pKa = 11.84RR159 pKa = 11.84LQVVPGLPPLEE170 pKa = 4.06MLLRR174 pKa = 11.84AVLHH178 pKa = 6.39RR179 pKa = 11.84ALAVAIHH186 pKa = 6.81LLHH189 pKa = 6.33QVFLGSLSCRR199 pKa = 11.84SVGKK203 pKa = 9.79LAQKK207 pKa = 10.43LL208 pKa = 3.71

Molecular weight: 23.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23671

11608

35279

21003233

17

7663

595.3

66.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.49 ± 0.012

2.257 ± 0.01

5.178 ± 0.009

6.832 ± 0.016

3.698 ± 0.009

6.318 ± 0.015

2.609 ± 0.007

4.383 ± 0.01

5.713 ± 0.015

9.486 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.005

3.909 ± 0.009

5.772 ± 0.019

4.716 ± 0.012

5.652 ± 0.011

8.914 ± 0.018

5.633 ± 0.014

6.275 ± 0.012

1.144 ± 0.004

2.694 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski