Streptococcus phage phiS10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1PSI3|M1PSI3_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage phiS10 OX=1289601 GN=phiS10_0026 PE=4 SV=1
MM1 pKa = 7.65NEE3 pKa = 4.09LKK5 pKa = 10.57EE6 pKa = 3.98KK7 pKa = 10.75ALAKK11 pKa = 10.19MLDD14 pKa = 3.72EE15 pKa = 4.41MNKK18 pKa = 8.91EE19 pKa = 3.99HH20 pKa = 7.01SPSEE24 pKa = 4.09DD25 pKa = 3.31HH26 pKa = 5.92VHH28 pKa = 6.21NWLCEE33 pKa = 3.81QEE35 pKa = 5.57DD36 pKa = 4.12DD37 pKa = 5.57DD38 pKa = 5.46GYY40 pKa = 11.46SYY42 pKa = 11.36CPYY45 pKa = 10.17CGYY48 pKa = 11.09ANEE51 pKa = 4.87LGEE54 pKa = 4.25EE55 pKa = 4.51DD56 pKa = 4.45YY57 pKa = 11.74EE58 pKa = 4.09EE59 pKa = 4.56TYY61 pKa = 11.16EE62 pKa = 5.05NEE64 pKa = 4.44TEE66 pKa = 4.31CGSCQEE72 pKa = 4.74IYY74 pKa = 10.79LEE76 pKa = 4.38SIEE79 pKa = 4.96IIVTRR84 pKa = 11.84YY85 pKa = 7.6TVTQRR90 pKa = 11.84LEE92 pKa = 4.5DD93 pKa = 3.44KK94 pKa = 10.68TKK96 pKa = 10.07AQQAADD102 pKa = 3.7EE103 pKa = 4.93LGWIVKK109 pKa = 8.73EE110 pKa = 4.43VEE112 pKa = 3.81

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1PSG7|M1PSG7_9CAUD Uncharacterized protein OS=Streptococcus phage phiS10 OX=1289601 GN=phiS10_006 PE=4 SV=1
MM1 pKa = 8.12KK2 pKa = 10.0IRR4 pKa = 11.84QKK6 pKa = 10.93PLIHH10 pKa = 6.98KK11 pKa = 8.4GFF13 pKa = 3.91

Molecular weight: 1.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10761

13

1045

199.3

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.611 ± 0.328

0.567 ± 0.125

6.254 ± 0.193

8.54 ± 0.554

4.014 ± 0.237

6.068 ± 0.532

1.44 ± 0.162

6.468 ± 0.248

8.085 ± 0.405

8.233 ± 0.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.286 ± 0.202

5.566 ± 0.273

2.583 ± 0.17

4.154 ± 0.182

4.925 ± 0.296

5.929 ± 0.257

5.678 ± 0.427

6.356 ± 0.281

1.403 ± 0.145

3.838 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski