Equine foamy virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Spumaretrovirinae; Equispumavirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9J4C5|Q9J4C5_9RETR ORF1/Tas/Bel1 OS=Equine foamy virus OX=109270 GN=tas PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84NPGLGCLTWTGQLNGMGRR20 pKa = 11.84FQIQLFRR27 pKa = 11.84NLTLISVCLGSDD39 pKa = 3.19HH40 pKa = 7.15HH41 pKa = 8.38KK42 pKa = 11.12GITDD46 pKa = 3.65YY47 pKa = 11.41CCGPASCYY55 pKa = 9.72TIVWEE60 pKa = 4.65ISAEE64 pKa = 4.11VLATGTPYY72 pKa = 11.03LFRR75 pKa = 11.84AQPHH79 pKa = 5.94HH80 pKa = 7.2RR81 pKa = 11.84PLQTPAPWSKK91 pKa = 10.32RR92 pKa = 11.84LIRR95 pKa = 11.84TRR97 pKa = 11.84YY98 pKa = 8.45IRR100 pKa = 11.84NSVEE104 pKa = 3.66DD105 pKa = 3.71QFVHH109 pKa = 6.91FATEE113 pKa = 3.69ANYY116 pKa = 9.91TPLSAQALSIKK127 pKa = 10.11AIRR130 pKa = 11.84WHH132 pKa = 6.6KK133 pKa = 10.28GQLACEE139 pKa = 4.07GTPRR143 pKa = 11.84IGQAGIPEE151 pKa = 4.72TIYY154 pKa = 11.11AGIKK158 pKa = 9.37AYY160 pKa = 10.19GWFIPGKK167 pKa = 9.21KK168 pKa = 9.52EE169 pKa = 3.56RR170 pKa = 11.84VSWNEE175 pKa = 3.53DD176 pKa = 3.42PEE178 pKa = 4.72TKK180 pKa = 10.54NIAIPSSTEE189 pKa = 3.67VQEE192 pKa = 4.16WFPMEE197 pKa = 5.06DD198 pKa = 2.86VDD200 pKa = 5.71DD201 pKa = 4.46RR202 pKa = 11.84ARR204 pKa = 11.84EE205 pKa = 3.94PLDD208 pKa = 3.52GSEE211 pKa = 4.06THH213 pKa = 6.42QEE215 pKa = 3.64IAEE218 pKa = 4.39FACAMLPPGWCIVRR232 pKa = 11.84PEE234 pKa = 3.7GRR236 pKa = 11.84TYY238 pKa = 10.94ISTGPDD244 pKa = 2.96TSDD247 pKa = 3.37EE248 pKa = 4.45EE249 pKa = 4.19EE250 pKa = 4.7DD251 pKa = 3.41KK252 pKa = 11.57ALAPKK257 pKa = 9.88ILKK260 pKa = 6.96THH262 pKa = 6.76PSICPKK268 pKa = 10.21HH269 pKa = 5.88PRR271 pKa = 11.84HH272 pKa = 6.07HH273 pKa = 6.9KK274 pKa = 8.89IGGRR278 pKa = 11.84FVSDD282 pKa = 4.14LLDD285 pKa = 4.09SPSSSDD291 pKa = 3.59DD292 pKa = 3.58EE293 pKa = 4.47EE294 pKa = 4.07EE295 pKa = 4.12ARR297 pKa = 11.84QNLARR302 pKa = 11.84VLEE305 pKa = 4.37AALPHH310 pKa = 6.58PGEE313 pKa = 5.19DD314 pKa = 4.79SSDD317 pKa = 3.89DD318 pKa = 3.59DD319 pKa = 4.13PLYY322 pKa = 10.87LGEE325 pKa = 4.57PEE327 pKa = 4.27RR328 pKa = 11.84DD329 pKa = 3.25

Molecular weight: 36.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9J4C8|Q9J4C8_9RETR Gag polyprotein OS=Equine foamy virus OX=109270 GN=gag PE=4 SV=1
MM1 pKa = 7.27AQNEE5 pKa = 4.52TFDD8 pKa = 3.89PVALQGYY15 pKa = 8.38YY16 pKa = 9.8PAGGILADD24 pKa = 3.75NDD26 pKa = 3.79IINIRR31 pKa = 11.84FTSGQWGIGDD41 pKa = 3.31RR42 pKa = 11.84WLQVRR47 pKa = 11.84LRR49 pKa = 11.84LVDD52 pKa = 4.04PNTGQPLAQPEE63 pKa = 4.28YY64 pKa = 10.28EE65 pKa = 4.4DD66 pKa = 3.41TGLPAEE72 pKa = 4.1NRR74 pKa = 11.84GIVVAVSHH82 pKa = 5.89NAARR86 pKa = 11.84NIFNNVQPAGGPNRR100 pKa = 11.84HH101 pKa = 6.06GPLHH105 pKa = 7.19DD106 pKa = 4.03GQFQVGDD113 pKa = 3.89DD114 pKa = 3.96PSEE117 pKa = 4.08HH118 pKa = 6.0FVPIEE123 pKa = 3.93EE124 pKa = 4.01NLIPQEE130 pKa = 4.01IVNLGAARR138 pKa = 11.84RR139 pKa = 11.84EE140 pKa = 3.95VRR142 pKa = 11.84LLRR145 pKa = 11.84EE146 pKa = 3.64MCVRR150 pKa = 11.84LLHH153 pKa = 6.49VRR155 pKa = 11.84RR156 pKa = 11.84QMMGMGMPGAIQPQPPVGPLPAPAQPPIPGPPVPPPVPPPAPPAPVNPPVPPVQPIHH213 pKa = 6.55HH214 pKa = 6.89LPITHH219 pKa = 6.28IRR221 pKa = 11.84AVIGEE226 pKa = 4.45TPAQIRR232 pKa = 11.84DD233 pKa = 3.61VPLWLAQSIPALTGVYY249 pKa = 9.66PAMDD253 pKa = 4.51AGTLTRR259 pKa = 11.84LVNAITARR267 pKa = 11.84HH268 pKa = 6.28PGLALGMNEE277 pKa = 3.53AGSWHH282 pKa = 6.31EE283 pKa = 4.02AVHH286 pKa = 6.66LIWQRR291 pKa = 11.84TFGATALHH299 pKa = 6.47ALSDD303 pKa = 3.94VLKK306 pKa = 11.17GIAQRR311 pKa = 11.84NGVVMALEE319 pKa = 4.28MGLMFTNDD327 pKa = 3.42DD328 pKa = 3.68WDD330 pKa = 4.17LTWSVIRR337 pKa = 11.84RR338 pKa = 11.84CLPGQASVVTIQARR352 pKa = 11.84LDD354 pKa = 3.63ALPNNQARR362 pKa = 11.84IIQAGFIIRR371 pKa = 11.84EE372 pKa = 4.21VYY374 pKa = 9.82EE375 pKa = 4.1VLGLDD380 pKa = 3.54PLGRR384 pKa = 11.84PLNFPGGLTQRR395 pKa = 11.84DD396 pKa = 3.33TAVPVTRR403 pKa = 11.84GRR405 pKa = 11.84GRR407 pKa = 11.84GRR409 pKa = 11.84TGPRR413 pKa = 11.84RR414 pKa = 11.84GPVLPVSSNQRR425 pKa = 11.84RR426 pKa = 11.84QEE428 pKa = 4.13TAGGNQPQTQPQQQNTFSNQTNQRR452 pKa = 11.84GNQRR456 pKa = 11.84QWQNRR461 pKa = 11.84GTDD464 pKa = 3.12SQRR467 pKa = 11.84RR468 pKa = 11.84YY469 pKa = 9.78FFRR472 pKa = 11.84PRR474 pKa = 11.84PSQPQRR480 pKa = 11.84YY481 pKa = 8.87GSNQGPDD488 pKa = 3.08NPNPYY493 pKa = 9.72RR494 pKa = 11.84GRR496 pKa = 11.84DD497 pKa = 3.38STNQSGQEE505 pKa = 3.78RR506 pKa = 11.84QLPQQQQGSRR516 pKa = 11.84RR517 pKa = 11.84GPGRR521 pKa = 11.84NTNSGNNTVHH531 pKa = 6.51TVRR534 pKa = 11.84QVEE537 pKa = 4.45SSQLQQNASPTASPSTNQGQQPP559 pKa = 3.38

Molecular weight: 61.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3276

249

1153

655.2

74.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.739 ± 0.519

1.496 ± 0.425

5.189 ± 0.378

5.037 ± 0.468

2.564 ± 0.089

5.952 ± 0.908

2.717 ± 0.136

6.746 ± 0.651

5.464 ± 1.666

9.799 ± 0.744

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.832 ± 0.255

4.67 ± 0.398

7.54 ± 0.95

6.441 ± 0.687

5.464 ± 1.161

5.403 ± 0.583

6.441 ± 0.544

5.586 ± 0.459

2.106 ± 0.168

3.816 ± 0.636
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski