Long Island tick rhabdovirus
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8QQQ8|W8QQQ8_9RHAB GDP polyribonucleotidyltransferase OS=Long Island tick rhabdovirus OX=1459044 PE=4 SV=1
MM1 pKa = 7.5 ASKK4 pKa = 10.62 RR5 pKa = 11.84 LDD7 pKa = 3.52 VNLPNCDD14 pKa = 3.43 KK15 pKa = 11.09 KK16 pKa = 11.24 SVNAGLPDD24 pKa = 3.56 TKK26 pKa = 10.97 VSVVYY31 pKa = 9.81 PSEE34 pKa = 3.84 WFKK37 pKa = 11.1 TNEE40 pKa = 3.78 GKK42 pKa = 10.65 KK43 pKa = 9.89 PIVRR47 pKa = 11.84 IYY49 pKa = 10.46 RR50 pKa = 11.84 ATSTEE55 pKa = 4.56 LYY57 pKa = 9.6 RR58 pKa = 11.84 ALYY61 pKa = 8.63 PQIKK65 pKa = 9.41 AEE67 pKa = 4.04 KK68 pKa = 8.84 WDD70 pKa = 4.06 DD71 pKa = 3.65 ALVTSFLVATLSEE84 pKa = 4.36 RR85 pKa = 11.84 PDD87 pKa = 3.55 LFRR90 pKa = 11.84 VEE92 pKa = 5.35 SDD94 pKa = 3.78 EE95 pKa = 4.03 EE96 pKa = 4.31 WVSFKK101 pKa = 10.76 QQLSVGGQVSVTDD114 pKa = 4.28 LLTLEE119 pKa = 4.33 EE120 pKa = 5.08 LEE122 pKa = 4.88 AKK124 pKa = 10.27 PPVPGPSKK132 pKa = 8.98 PTPAEE137 pKa = 4.2 EE138 pKa = 4.15 ISFFCALLTIYY149 pKa = 10.44 RR150 pKa = 11.84 RR151 pKa = 11.84 VQARR155 pKa = 11.84 EE156 pKa = 3.76 NPAVKK161 pKa = 10.47 ASDD164 pKa = 3.55 LTDD167 pKa = 2.99 KK168 pKa = 10.85 FKK170 pKa = 11.36 NVLGRR175 pKa = 11.84 EE176 pKa = 4.33 PYY178 pKa = 11.0 NMSDD182 pKa = 2.98 EE183 pKa = 4.65 DD184 pKa = 3.9 ARR186 pKa = 11.84 LMVPIAGTANLTSGFMRR203 pKa = 11.84 ILSVIDD209 pKa = 3.17 MFFVKK214 pKa = 10.26 FPQAPGSAVRR224 pKa = 11.84 IATFTLRR231 pKa = 11.84 YY232 pKa = 9.05 RR233 pKa = 11.84 GCTAITALTHH243 pKa = 6.21 ACITLGLDD251 pKa = 3.52 AYY253 pKa = 10.47 DD254 pKa = 4.07 LASLIVPQSPADD266 pKa = 3.63 DD267 pKa = 3.4 AVRR270 pKa = 11.84 VYY272 pKa = 11.34 EE273 pKa = 5.41 DD274 pKa = 3.88 GQEE277 pKa = 3.96 HH278 pKa = 7.79 DD279 pKa = 4.63 DD280 pKa = 3.99 PYY282 pKa = 11.67 GYY284 pKa = 10.41 FPYY287 pKa = 10.02 HH288 pKa = 5.94 FVMGLSNHH296 pKa = 5.74 SPYY299 pKa = 11.17 SLAAAPSLTFYY310 pKa = 9.64 TRR312 pKa = 11.84 CLAAFGGSKK321 pKa = 9.69 NAAYY325 pKa = 8.53 TYY327 pKa = 8.81 TAAGIRR333 pKa = 11.84 NVPEE337 pKa = 4.86 LIEE340 pKa = 3.85 TAYY343 pKa = 11.39 LLIKK347 pKa = 9.96 KK348 pKa = 9.41 IRR350 pKa = 11.84 RR351 pKa = 11.84 LEE353 pKa = 4.28 PGDD356 pKa = 3.86 LRR358 pKa = 11.84 FAEE361 pKa = 4.59 SSAMDD366 pKa = 2.9 KK367 pKa = 10.51 WKK369 pKa = 10.43 AHH371 pKa = 5.95 KK372 pKa = 9.54 EE373 pKa = 4.01 ARR375 pKa = 11.84 DD376 pKa = 3.76 KK377 pKa = 11.24 LLKK380 pKa = 10.64 DD381 pKa = 3.71 DD382 pKa = 6.22 DD383 pKa = 4.28 EE384 pKa = 7.18 DD385 pKa = 3.82 EE386 pKa = 4.53 VQLHH390 pKa = 6.47 EE391 pKa = 4.61 GAQEE395 pKa = 4.05 RR396 pKa = 11.84 GLVASLLALPTRR408 pKa = 11.84 QEE410 pKa = 3.88 LTPLHH415 pKa = 6.3 RR416 pKa = 11.84 RR417 pKa = 11.84 IDD419 pKa = 3.53 EE420 pKa = 4.38 ALAEE424 pKa = 4.28 LSVHH428 pKa = 6.53 HH429 pKa = 6.26 GLSRR433 pKa = 11.84 KK434 pKa = 9.04 NAMGLYY440 pKa = 9.94 HH441 pKa = 7.72 AAQCFKK447 pKa = 10.58 GADD450 pKa = 3.53 VGTVGHH456 pKa = 7.06 TIYY459 pKa = 10.57 DD460 pKa = 3.34 QYY462 pKa = 11.68 GRR464 pKa = 11.84 VPPYY468 pKa = 10.84 VEE470 pKa = 3.8
Molecular weight: 52.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.666
IPC2_protein 5.664
IPC_protein 5.703
Toseland 6.059
ProMoST 5.906
Dawson 5.919
Bjellqvist 5.944
Wikipedia 5.906
Rodwell 5.893
Grimsley 6.198
Solomon 5.919
Lehninger 5.906
Nozaki 6.173
DTASelect 6.351
Thurlkill 6.338
EMBOSS 6.3
Sillero 6.262
Patrickios 4.113
IPC_peptide 5.931
IPC2_peptide 6.249
IPC2.peptide.svr19 6.254
Protein with the highest isoelectric point:
>tr|W8R902|W8R902_9RHAB Glycoprotein OS=Long Island tick rhabdovirus OX=1459044 PE=3 SV=1
MM1 pKa = 7.26 LRR3 pKa = 11.84 YY4 pKa = 10.05 AFVLLMIAVAFGWEE18 pKa = 4.22 PEE20 pKa = 4.12 LGEE23 pKa = 4.18 YY24 pKa = 8.21 WVPSPISPWRR34 pKa = 11.84 LATKK38 pKa = 10.07 TDD40 pKa = 4.13 FTCPSIRR47 pKa = 11.84 VDD49 pKa = 3.29 PVSPLKK55 pKa = 10.67 AINEE59 pKa = 4.51 SYY61 pKa = 11.06 VEE63 pKa = 4.11 YY64 pKa = 8.64 PTMGGARR71 pKa = 11.84 KK72 pKa = 7.97 EE73 pKa = 4.2 HH74 pKa = 6.71 PGWLCIRR81 pKa = 11.84 KK82 pKa = 7.75 TYY84 pKa = 8.73 QTTCDD89 pKa = 3.34 TNFWGHH95 pKa = 4.54 QTIKK99 pKa = 10.46 HH100 pKa = 5.42 EE101 pKa = 4.19 EE102 pKa = 3.75 WSIVADD108 pKa = 3.98 VEE110 pKa = 4.24 EE111 pKa = 4.53 CRR113 pKa = 11.84 QAVSHH118 pKa = 5.75 YY119 pKa = 10.7 QLGTYY124 pKa = 8.77 EE125 pKa = 4.83 DD126 pKa = 4.42 PRR128 pKa = 11.84 HH129 pKa = 6.49 PDD131 pKa = 3.59 PVCTWMAISSTYY143 pKa = 10.25 RR144 pKa = 11.84 AGTLLLPHH152 pKa = 6.32 TTLVDD157 pKa = 3.53 PFSYY161 pKa = 10.43 TFVDD165 pKa = 3.57 SLFPGTHH172 pKa = 6.88 CKK174 pKa = 9.73 TIPCMTVHH182 pKa = 7.51 PDD184 pKa = 3.81 VLWHH188 pKa = 6.28 SSSTITEE195 pKa = 4.22 NCPMANGIHH204 pKa = 6.1 IKK206 pKa = 10.43 LYY208 pKa = 9.5 NDD210 pKa = 2.47 NRR212 pKa = 11.84 ARR214 pKa = 11.84 KK215 pKa = 8.67 KK216 pKa = 8.68 AHH218 pKa = 6.05 EE219 pKa = 4.21 WLSVNDD225 pKa = 4.57 GPLIPLGGSCSMHH238 pKa = 6.05 YY239 pKa = 10.17 CGQAGLRR246 pKa = 11.84 TPGGIWYY253 pKa = 8.4 PYY255 pKa = 10.23 LGRR258 pKa = 11.84 QKK260 pKa = 10.9 YY261 pKa = 8.78 PEE263 pKa = 4.61 CKK265 pKa = 9.18 EE266 pKa = 3.65 AWRR269 pKa = 11.84 ATPTPAGNGAKK280 pKa = 10.03 LDD282 pKa = 3.71 IEE284 pKa = 4.24 LQEE287 pKa = 4.3 LIARR291 pKa = 11.84 RR292 pKa = 11.84 EE293 pKa = 4.16 CLNVLQQIRR302 pKa = 11.84 SGVSPTFHH310 pKa = 6.62 MLSHH314 pKa = 6.37 FQPRR318 pKa = 11.84 HH319 pKa = 4.21 TGYY322 pKa = 10.6 YY323 pKa = 8.77 RR324 pKa = 11.84 VYY326 pKa = 10.75 RR327 pKa = 11.84 MNNGLVEE334 pKa = 4.15 YY335 pKa = 10.39 SLALYY340 pKa = 10.54 APIFNITMPPSINFGVRR357 pKa = 11.84 RR358 pKa = 11.84 NKK360 pKa = 10.07 SRR362 pKa = 11.84 YY363 pKa = 7.6 HH364 pKa = 7.58 LPMIEE369 pKa = 4.16 SGHH372 pKa = 6.13 PGVWSAFNGIHH383 pKa = 6.21 IMHH386 pKa = 7.24 NKK388 pKa = 7.72 TVIVPEE394 pKa = 3.99 LEE396 pKa = 4.15 LYY398 pKa = 10.01 KK399 pKa = 10.46 EE400 pKa = 4.75 HH401 pKa = 7.38 YY402 pKa = 10.33 SEE404 pKa = 4.02 TLFYY408 pKa = 11.24 YY409 pKa = 10.41 KK410 pKa = 10.49 AQLAEE415 pKa = 4.47 HH416 pKa = 7.26 PSQVRR421 pKa = 11.84 QANSTDD427 pKa = 3.12 ITPHH431 pKa = 5.71 TKK433 pKa = 8.11 TTVTVKK439 pKa = 10.63 RR440 pKa = 11.84 PTLKK444 pKa = 10.58 AWFSTMWDD452 pKa = 3.82 SLWGKK457 pKa = 9.15 VVSITGIILAIFTTGFLIKK476 pKa = 10.16 VLPWSRR482 pKa = 11.84 LFRR485 pKa = 11.84 RR486 pKa = 11.84 PQPMHH491 pKa = 6.12 PQIVHH496 pKa = 4.89 YY497 pKa = 9.21 TPATGSVSWW506 pKa = 3.73
Molecular weight: 57.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.912
IPC2_protein 8.009
IPC_protein 7.834
Toseland 7.571
ProMoST 8.375
Dawson 8.521
Bjellqvist 8.858
Wikipedia 8.478
Rodwell 8.551
Grimsley 7.644
Solomon 8.595
Lehninger 8.595
Nozaki 8.96
DTASelect 8.595
Thurlkill 8.639
EMBOSS 8.712
Sillero 8.931
Patrickios 3.834
IPC_peptide 8.58
IPC2_peptide 7.922
IPC2.peptide.svr19 7.987
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3588
205
2131
717.6
81.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.048 ± 0.897
1.895 ± 0.218
5.295 ± 0.567
6.048 ± 0.316
3.484 ± 0.35
6.159 ± 0.225
2.982 ± 0.451
4.849 ± 0.405
5.072 ± 0.294
10.117 ± 0.858
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.341 ± 0.201
3.372 ± 0.384
6.187 ± 1.057
3.122 ± 0.135
6.494 ± 0.346
7.079 ± 0.241
6.745 ± 0.457
7.023 ± 0.547
2.09 ± 0.35
3.595 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here