bacterium E08(2017)
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1267 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A202DKA1|A0A202DKA1_9BACT Uncharacterized protein OS=bacterium E08(2017) OX=1932693 GN=BVX97_01230 PE=4 SV=1
MM1 pKa = 7.68 SFRR4 pKa = 11.84 KK5 pKa = 10.12 LSPKK9 pKa = 9.57 LRR11 pKa = 11.84 LMIVSFVVMSFVFVGIALDD30 pKa = 3.68 TDD32 pKa = 3.73 KK33 pKa = 11.74 DD34 pKa = 3.89 GMSDD38 pKa = 3.36 NFEE41 pKa = 4.88 LLFDD45 pKa = 5.37 LNPNNQEE52 pKa = 4.29 DD53 pKa = 3.88 ADD55 pKa = 4.3 LNYY58 pKa = 11.18 DD59 pKa = 3.47 SDD61 pKa = 3.99 NLINSEE67 pKa = 4.29 EE68 pKa = 4.26 YY69 pKa = 11.4 ANATDD74 pKa = 3.44 PWEE77 pKa = 4.32 TDD79 pKa = 2.65 TDD81 pKa = 3.99 RR82 pKa = 11.84 DD83 pKa = 3.67 GWSDD87 pKa = 4.81 DD88 pKa = 3.37 VDD90 pKa = 3.8 GDD92 pKa = 3.64 PTSRR96 pKa = 11.84 LFIDD100 pKa = 3.42 WGEE103 pKa = 3.95 PLYY106 pKa = 9.94 TFCDD110 pKa = 4.09 DD111 pKa = 3.72 YY112 pKa = 11.81 VYY114 pKa = 10.55 TGPLWWMSAFKK125 pKa = 10.94 LGGEE129 pKa = 4.34 WVATTNFTSWYY140 pKa = 9.11 SGNASDD146 pKa = 5.42 RR147 pKa = 11.84 VMIEE151 pKa = 4.15 LDD153 pKa = 3.26 RR154 pKa = 11.84 SLITQPVVLDD164 pKa = 3.38 MDD166 pKa = 5.59 LYY168 pKa = 9.73 SQNGSAVSVDD178 pKa = 4.24 LLDD181 pKa = 5.46 ANDD184 pKa = 3.96 SVVASDD190 pKa = 3.84 IFGNTIAVPAAKK202 pKa = 9.84 SVSRR206 pKa = 11.84 SVDD209 pKa = 2.81 INLPLYY215 pKa = 10.85 SDD217 pKa = 4.03 AASIAIGPAFGDD229 pKa = 3.33 LSIFQTHH236 pKa = 5.87 VYY238 pKa = 10.2 VDD240 pKa = 3.67 QDD242 pKa = 3.25 HH243 pKa = 7.63 DD244 pKa = 3.98 GLDD247 pKa = 3.88 AEE249 pKa = 5.17 QEE251 pKa = 4.3 AQLGTSDD258 pKa = 5.42 LSADD262 pKa = 3.58 SDD264 pKa = 4.21 GDD266 pKa = 3.71 GMSDD270 pKa = 3.66 YY271 pKa = 11.62 EE272 pKa = 4.16 EE273 pKa = 4.15 VFYY276 pKa = 10.84 IGSDD280 pKa = 3.49 PSSAPMIAPVVAGGGAGDD298 pKa = 3.88 PEE300 pKa = 5.59 GYY302 pKa = 10.62 ASLIATQTVAGSQDD316 pKa = 2.91 INLASVTALDD326 pKa = 3.9 WVGSSVDD333 pKa = 3.45 ANKK336 pKa = 8.78 FTVGANGVITVTDD349 pKa = 3.59 AGDD352 pKa = 3.65 YY353 pKa = 9.51 MIAAALPMYY362 pKa = 10.46 SAITRR367 pKa = 11.84 SCVRR371 pKa = 11.84 LQAYY375 pKa = 10.26 VNGTVVPGLVGEE387 pKa = 4.29 SSYY390 pKa = 11.23 VRR392 pKa = 11.84 NTSGHH397 pKa = 5.87 NEE399 pKa = 4.25 SSCHH403 pKa = 5.67 FAGLLKK409 pKa = 10.61 GLNAGDD415 pKa = 3.74 QIEE418 pKa = 4.5 VKK420 pKa = 10.86 VLGTAVAGSGVSMSTARR437 pKa = 11.84 LYY439 pKa = 10.44 MEE441 pKa = 6.27 AIDD444 pKa = 4.05 ASRR447 pKa = 11.84 VVYY450 pKa = 10.6 SGLSDD455 pKa = 3.84 GPAGGDD461 pKa = 3.45 VNPATAAPMLWTTDD475 pKa = 3.24 QRR477 pKa = 11.84 IDD479 pKa = 3.57 SGFTHH484 pKa = 6.66 SAGSQSITLDD494 pKa = 3.47 DD495 pKa = 4.18 GGVYY499 pKa = 10.47 LVYY502 pKa = 10.79 FNMPVYY508 pKa = 9.96 STVSRR513 pKa = 11.84 SAPRR517 pKa = 11.84 IEE519 pKa = 3.96 MKK521 pKa = 10.14 IDD523 pKa = 3.0 GVMVAGGAGKK533 pKa = 9.86 QGYY536 pKa = 9.1 IKK538 pKa = 10.71 GGSHH542 pKa = 7.0 SDD544 pKa = 3.89 ASVHH548 pKa = 4.64 WSGVIQASAGSVLTVDD564 pKa = 4.31 TMQEE568 pKa = 3.45 AAAGVVTVPAGEE580 pKa = 4.16 KK581 pKa = 10.82 AMLTIEE587 pKa = 4.81 KK588 pKa = 9.95 LDD590 pKa = 3.87 ASQDD594 pKa = 3.57 VYY596 pKa = 11.25 FGLGISLDD604 pKa = 4.33 SGDD607 pKa = 3.68 NWNVAGSVQFADD619 pKa = 4.47 QIIDD623 pKa = 3.39 GTKK626 pKa = 7.92 YY627 pKa = 10.65 SKK629 pKa = 10.39 PATHH633 pKa = 5.88 QVQVSEE639 pKa = 4.38 AGDD642 pKa = 3.58 YY643 pKa = 11.3 LLVYY647 pKa = 10.35 NDD649 pKa = 3.7 SFYY652 pKa = 11.28 ATLSRR657 pKa = 11.84 ANPKK661 pKa = 8.73 VTVTVNGAAVPGAEE675 pKa = 4.39 VKK677 pKa = 10.18 SHH679 pKa = 7.3 HH680 pKa = 6.46 IRR682 pKa = 11.84 NSSGHH687 pKa = 5.67 NEE689 pKa = 4.05 SSGSLVTMLDD699 pKa = 3.43 GLSAGDD705 pKa = 3.56 VVAIALEE712 pKa = 4.13 QEE714 pKa = 4.2 AAGGVVDD721 pKa = 5.61 DD722 pKa = 5.45 DD723 pKa = 4.53 APAVLALISKK733 pKa = 10.54 AGGQVGPQAPVANAGADD750 pKa = 3.62 QNVEE754 pKa = 4.1 DD755 pKa = 4.36 TDD757 pKa = 4.5 GNGSEE762 pKa = 4.64 SVSLDD767 pKa = 3.12 GSGSTDD773 pKa = 2.92 SDD775 pKa = 3.75 GTIVSYY781 pKa = 10.94 SWTTGATEE789 pKa = 3.86 IATGVAPVVSLPVGANVVTLTVEE812 pKa = 4.48 DD813 pKa = 4.61 DD814 pKa = 4.37 GGLQDD819 pKa = 4.15 SDD821 pKa = 4.01 TVVITVNPAPANEE834 pKa = 4.25 APVASFTAAPGSGSAPLDD852 pKa = 3.49 VAFDD856 pKa = 4.03 ASASYY861 pKa = 11.19 DD862 pKa = 3.72 PDD864 pKa = 3.96 GSIADD869 pKa = 4.05 YY870 pKa = 10.9 AWDD873 pKa = 3.84 YY874 pKa = 11.79 GDD876 pKa = 3.93 GAVEE880 pKa = 4.27 NGASLITPSHH890 pKa = 7.09 TYY892 pKa = 8.71 TADD895 pKa = 3.14 GSYY898 pKa = 10.75 SAVLVVTDD906 pKa = 5.23 DD907 pKa = 3.94 EE908 pKa = 5.1 GATATNTTTITVGDD922 pKa = 3.98 APEE925 pKa = 4.91 GGSFASLTASQTVAGDD941 pKa = 3.63 QNVNTATATALDD953 pKa = 4.03 WVGSSVDD960 pKa = 3.45 ANKK963 pKa = 8.78 FTVGANGVITVADD976 pKa = 3.99 AGDD979 pKa = 3.75 YY980 pKa = 9.82 MIAATLPMNGPVYY993 pKa = 9.97 RR994 pKa = 11.84 SCVRR998 pKa = 11.84 MQAYY1002 pKa = 10.07 VNGVAVPGLLGEE1014 pKa = 4.19 SSYY1017 pKa = 11.11 IRR1019 pKa = 11.84 DD1020 pKa = 3.36 SSGHH1024 pKa = 5.76 NEE1026 pKa = 4.21 SSSHH1030 pKa = 5.59 FAGLLAGLNANDD1042 pKa = 4.09 EE1043 pKa = 4.39 IQVKK1047 pKa = 10.24 VLGTAGSGVITMDD1060 pKa = 3.76 GARR1063 pKa = 11.84 LYY1065 pKa = 11.16 LEE1067 pKa = 5.78 AIDD1070 pKa = 4.44 ASRR1073 pKa = 11.84 AVYY1076 pKa = 10.37 SGLSDD1081 pKa = 3.84 GPAGGDD1087 pKa = 3.45 VNPATAAPMLWTTDD1101 pKa = 3.24 QRR1103 pKa = 11.84 IDD1105 pKa = 3.57 SGFTHH1110 pKa = 6.66 SAGSQSITLDD1120 pKa = 3.16 ADD1122 pKa = 3.53 GLYY1125 pKa = 10.53 LVYY1128 pKa = 10.57 FNMPIYY1134 pKa = 10.03 STVARR1139 pKa = 11.84 SAPRR1143 pKa = 11.84 IEE1145 pKa = 4.23 VKK1147 pKa = 10.73 LDD1149 pKa = 3.0 GVMVAGGAGKK1159 pKa = 10.17 QGDD1162 pKa = 3.75 IKK1164 pKa = 11.01 SGSGHH1169 pKa = 6.51 NDD1171 pKa = 2.82 ASVHH1175 pKa = 4.31 WSGVIQATAGSVLTVDD1191 pKa = 4.25 TMQEE1195 pKa = 3.81 ATLGTVTVPAGEE1207 pKa = 4.18 KK1208 pKa = 10.88 AMLTVEE1214 pKa = 5.19 KK1215 pKa = 10.19 LDD1217 pKa = 3.86 EE1218 pKa = 4.39 SQGVYY1223 pKa = 10.3 FGLGYY1228 pKa = 8.98 TVDD1231 pKa = 5.75 GGDD1234 pKa = 3.31 SWNVAGNVDD1243 pKa = 4.61 FSDD1246 pKa = 5.63 LIIDD1250 pKa = 3.56 GTKK1253 pKa = 9.3 YY1254 pKa = 10.67 SKK1256 pKa = 10.12 PANHH1260 pKa = 5.64 QVQVSEE1266 pKa = 4.33 AGDD1269 pKa = 3.58 YY1270 pKa = 11.3 LLVYY1274 pKa = 10.35 NDD1276 pKa = 3.39 SFYY1279 pKa = 11.25 ATVARR1284 pKa = 11.84 ANPKK1288 pKa = 8.81 VTVTVNGVPVAGAEE1302 pKa = 4.39 VKK1304 pKa = 10.1 SHH1306 pKa = 6.55 YY1307 pKa = 9.73 IRR1309 pKa = 11.84 NSGGHH1314 pKa = 5.65 NEE1316 pKa = 4.22 SSGSLVTMLDD1326 pKa = 3.43 GLSAGDD1332 pKa = 3.56 VVAIALEE1339 pKa = 4.04 QEE1341 pKa = 3.97 AAAGIVDD1348 pKa = 4.43 DD1349 pKa = 5.35 DD1350 pKa = 4.46 APAVLALILKK1360 pKa = 7.49 TAGPVGPQPPLANAGADD1377 pKa = 3.53 QAVEE1381 pKa = 4.08 DD1382 pKa = 4.68 ADD1384 pKa = 4.89 DD1385 pKa = 4.24 NGSEE1389 pKa = 4.45 SVTLDD1394 pKa = 3.24 GTGSTDD1400 pKa = 3.01 SDD1402 pKa = 3.9 GTIVSYY1408 pKa = 10.93 SWTTGTTEE1416 pKa = 3.96 IATGAAPVVTLPVGTNVVTLTVEE1439 pKa = 4.84 DD1440 pKa = 3.89 NDD1442 pKa = 4.04 GLQDD1446 pKa = 3.45 SDD1448 pKa = 4.17 VVVITVNPFVAVNVPPAADD1467 pKa = 3.29 AGVDD1471 pKa = 3.66 QNVEE1475 pKa = 4.1 DD1476 pKa = 4.0 TDD1478 pKa = 4.5 GNGSEE1483 pKa = 4.64 SVSLDD1488 pKa = 3.12 GSGSTDD1494 pKa = 2.92 SDD1496 pKa = 3.75 GTIVSYY1502 pKa = 10.94 SWTTGATEE1510 pKa = 3.86 IATGVAPVVSLPVGANVVTLTVEE1533 pKa = 4.48 DD1534 pKa = 4.61 DD1535 pKa = 4.37 GGLQDD1540 pKa = 4.15 SDD1542 pKa = 4.01 TVVITVNPAPANEE1555 pKa = 4.25 APVASFTAAPGSGSAPLDD1573 pKa = 3.49 VAFDD1577 pKa = 4.03 ASASYY1582 pKa = 11.19 DD1583 pKa = 3.72 PDD1585 pKa = 3.96 GSIADD1590 pKa = 4.05 YY1591 pKa = 10.9 AWDD1594 pKa = 3.84 YY1595 pKa = 11.79 GDD1597 pKa = 3.93 GAVEE1601 pKa = 4.27 NGASLITPSHH1611 pKa = 7.09 TYY1613 pKa = 8.71 TADD1616 pKa = 3.14 GSYY1619 pKa = 10.75 SAVLVVTDD1627 pKa = 5.23 DD1628 pKa = 3.94 EE1629 pKa = 5.1 GATATNTTTITVGDD1643 pKa = 3.98 APEE1646 pKa = 4.91 GGSFASLTASQTVAGDD1662 pKa = 3.63 QNVNTATATALDD1674 pKa = 4.03 WVGSSVDD1681 pKa = 3.45 ANKK1684 pKa = 8.78 FTVGANGVITVADD1697 pKa = 3.99 AGDD1700 pKa = 3.75 YY1701 pKa = 9.82 MIAATLPMNGPVYY1714 pKa = 9.97 RR1715 pKa = 11.84 SCVRR1719 pKa = 11.84 MQAYY1723 pKa = 10.07 VNGVAVPGLLGEE1735 pKa = 4.19 SSYY1738 pKa = 11.11 IRR1740 pKa = 11.84 DD1741 pKa = 3.36 SSGHH1745 pKa = 5.76 NEE1747 pKa = 4.21 SSSHH1751 pKa = 5.59 FAGLLAGLNANDD1763 pKa = 4.09 EE1764 pKa = 4.39 IQVKK1768 pKa = 10.24 VLGTAGSGVITMDD1781 pKa = 3.76 GARR1784 pKa = 11.84 LYY1786 pKa = 11.16 LEE1788 pKa = 5.78 AIDD1791 pKa = 4.44 ASRR1794 pKa = 11.84 AVYY1797 pKa = 10.37 SGLSDD1802 pKa = 3.84 GPAGGDD1808 pKa = 3.45 VNPATAAPMLWTTDD1822 pKa = 3.24 QRR1824 pKa = 11.84 IDD1826 pKa = 3.57 SGFTHH1831 pKa = 6.66 SAGSQSITLDD1841 pKa = 3.16 ADD1843 pKa = 3.53 GLYY1846 pKa = 10.53 LVYY1849 pKa = 10.57 FNMPIYY1855 pKa = 10.03 STVARR1860 pKa = 11.84 SAPRR1864 pKa = 11.84 IEE1866 pKa = 4.23 VKK1868 pKa = 10.73 LDD1870 pKa = 3.0 GVMVAGGAGKK1880 pKa = 9.88 QGYY1883 pKa = 8.46 IKK1885 pKa = 10.58 SGSGHH1890 pKa = 6.47 NDD1892 pKa = 2.82 ASVHH1896 pKa = 4.31 WSGVIQATAGSVLTVDD1912 pKa = 4.25 TMQEE1916 pKa = 3.81 ATLGTVTVPAGEE1928 pKa = 4.18 KK1929 pKa = 10.88 AMLTVEE1935 pKa = 5.19 KK1936 pKa = 10.19 LDD1938 pKa = 3.86 EE1939 pKa = 4.39 SQGVYY1944 pKa = 10.3 FGLGYY1949 pKa = 8.98 TVDD1952 pKa = 5.75 GGDD1955 pKa = 3.31 SWNVAGNVDD1964 pKa = 4.61 FSDD1967 pKa = 5.63 LIIDD1971 pKa = 3.56 GTKK1974 pKa = 9.3 YY1975 pKa = 10.67 SKK1977 pKa = 10.12 PANHH1981 pKa = 5.64 QVQVSEE1987 pKa = 4.33 AGDD1990 pKa = 3.58 YY1991 pKa = 11.3 LLVYY1995 pKa = 10.35 NDD1997 pKa = 3.39 SFYY2000 pKa = 11.25 ATVARR2005 pKa = 11.84 ANPKK2009 pKa = 8.81 VTVTVNGVPVAGAEE2023 pKa = 4.39 VKK2025 pKa = 10.1 SHH2027 pKa = 6.55 YY2028 pKa = 9.73 IRR2030 pKa = 11.84 NSGGHH2035 pKa = 5.65 NEE2037 pKa = 4.22 SSGSLVTMLDD2047 pKa = 3.43 GLSAGDD2053 pKa = 3.56 VVAIALEE2060 pKa = 4.04 QEE2062 pKa = 3.97 AAAGIVDD2069 pKa = 4.43 DD2070 pKa = 5.35 DD2071 pKa = 4.46 APAVLALISKK2081 pKa = 9.65 VSVPTPDD2088 pKa = 4.33 PDD2090 pKa = 4.05 SDD2092 pKa = 4.11 GDD2094 pKa = 4.32 GMDD2097 pKa = 4.87 DD2098 pKa = 3.62 DD2099 pKa = 4.83 WEE2101 pKa = 4.14 ILHH2104 pKa = 6.76 FGNLDD2109 pKa = 3.31 QTAEE2113 pKa = 3.95 GDD2115 pKa = 3.43 YY2116 pKa = 11.34 DD2117 pKa = 4.49 ADD2119 pKa = 3.54 GRR2121 pKa = 11.84 TNLQEE2126 pKa = 4.2 FQHH2129 pKa = 5.9 GTVPTDD2135 pKa = 3.63 DD2136 pKa = 3.95 QDD2138 pKa = 3.87 YY2139 pKa = 10.04 QANLTFHH2146 pKa = 6.96 VDD2148 pKa = 3.23 SLLGNDD2154 pKa = 4.22 ANPGTSEE2161 pKa = 5.15 LPFATIGRR2169 pKa = 11.84 AVGLSIDD2176 pKa = 3.64 GDD2178 pKa = 4.34 LIEE2181 pKa = 4.89 VASGTYY2187 pKa = 9.84 PEE2189 pKa = 5.71 LPTTYY2194 pKa = 10.94 DD2195 pKa = 3.38 FTDD2198 pKa = 3.45 KK2199 pKa = 10.88 NVTMFSTGPYY2209 pKa = 10.01 SVTPP2213 pKa = 3.98
Molecular weight: 227.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.579
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.63
Grimsley 3.478
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A202DM30|A0A202DM30_9BACT 30S ribosomal protein S17 OS=bacterium E08(2017) OX=1932693 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.57 AKK3 pKa = 9.87 KK4 pKa = 10.41 ALIAKK9 pKa = 8.4 SEE11 pKa = 4.11 RR12 pKa = 11.84 KK13 pKa = 8.92 PKK15 pKa = 10.56 YY16 pKa = 9.96 GVRR19 pKa = 11.84 KK20 pKa = 9.13 YY21 pKa = 10.54 NRR23 pKa = 11.84 CKK25 pKa = 9.72 RR26 pKa = 11.84 CGRR29 pKa = 11.84 PRR31 pKa = 11.84 AYY33 pKa = 10.13 LRR35 pKa = 11.84 KK36 pKa = 9.71 FEE38 pKa = 4.39 LCRR41 pKa = 11.84 ICFRR45 pKa = 11.84 EE46 pKa = 4.07 LASHH50 pKa = 6.33 GKK52 pKa = 8.98 IPGVIKK58 pKa = 10.76 ASWW61 pKa = 2.84
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.882
IPC_protein 10.526
Toseland 10.921
ProMoST 10.526
Dawson 10.994
Bjellqvist 10.672
Wikipedia 11.169
Rodwell 11.374
Grimsley 11.023
Solomon 11.096
Lehninger 11.067
Nozaki 10.906
DTASelect 10.657
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.921
Patrickios 11.111
IPC_peptide 11.111
IPC2_peptide 9.765
IPC2.peptide.svr19 8.428
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1267
0
1267
424508
30
3334
335.0
37.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.23 ± 0.071
1.226 ± 0.029
6.373 ± 0.067
6.661 ± 0.076
3.809 ± 0.046
7.626 ± 0.082
1.946 ± 0.034
6.46 ± 0.05
5.815 ± 0.088
9.003 ± 0.083
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.035
4.011 ± 0.052
4.109 ± 0.044
2.977 ± 0.039
4.973 ± 0.066
6.715 ± 0.067
5.497 ± 0.082
7.181 ± 0.058
1.314 ± 0.029
3.302 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here