Saccharopolyspora flava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia<

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1I6TKP3|A0A1I6TKP3_9PSEU Sarcosine oxidase subunit delta OS=Saccharopolyspora flava OX=95161 GN=SAMN05660874_04016 PE=4 SV=1
MM1 pKa = 7.39LASGAQFGPIVIRR14 pKa = 11.84GNAPATGTSVTNTASVAGADD34 pKa = 3.63VDD36 pKa = 5.5SNTANNTASATTSLTAQAPALTVQKK61 pKa = 8.81TANPTSVTTAGAPIVYY77 pKa = 9.65SYY79 pKa = 10.92RR80 pKa = 11.84VSNIGNVPLTGISVAEE96 pKa = 4.04TAFTGTGPRR105 pKa = 11.84PSASCPATSLDD116 pKa = 3.67PGASMTCTANYY127 pKa = 7.57TATQADD133 pKa = 4.0VDD135 pKa = 4.11RR136 pKa = 11.84GSIVNTAVANGTSPAGVAVVSPPTTATVTAAASPSLTVAKK176 pKa = 9.03TASPSDD182 pKa = 3.32AASFTVGRR190 pKa = 11.84VITYY194 pKa = 9.1SYY196 pKa = 10.8RR197 pKa = 11.84ITNTGNVTLTNVRR210 pKa = 11.84PTDD213 pKa = 3.39TAFNGSGPRR222 pKa = 11.84PTPTCPSGAASLAPGASVTCTATYY246 pKa = 9.53TVTQADD252 pKa = 3.77VDD254 pKa = 4.24RR255 pKa = 11.84GSLTNTATATGTPPSGSSPPTSPPSTVTVPGDD287 pKa = 3.49SAPALTVRR295 pKa = 11.84KK296 pKa = 9.15SASPTTMNAAGNTITYY312 pKa = 9.97RR313 pKa = 11.84FLVTNTGNVTLNSVSIAEE331 pKa = 4.16PTFTGTGGAPAVTCPSGPLLPGAQTTCTATYY362 pKa = 10.67SVTQADD368 pKa = 4.27MDD370 pKa = 4.3RR371 pKa = 11.84GTIDD375 pKa = 3.2NTATASAVAPDD386 pKa = 3.62ATRR389 pKa = 11.84VTSAPSSARR398 pKa = 11.84VTADD402 pKa = 3.7RR403 pKa = 11.84APALAVVKK411 pKa = 10.33SATPTTVTAAGDD423 pKa = 3.49AVNYY427 pKa = 9.09SFRR430 pKa = 11.84VTNTGNVSLDD440 pKa = 3.82TITVADD446 pKa = 4.04TQFSGTGTPPAPSCPPGVLAPGASTTCTASYY477 pKa = 8.51TATQADD483 pKa = 3.73IDD485 pKa = 4.35AGSISNTATASGRR498 pKa = 11.84DD499 pKa = 3.67PTTGNGTTSPPSSATVIADD518 pKa = 3.67ATPGLSLVKK527 pKa = 9.56TADD530 pKa = 3.51PTRR533 pKa = 11.84VTAAGQTVTFGFLVTNTSAVTLNDD557 pKa = 3.58PQIAEE562 pKa = 4.1TDD564 pKa = 4.02FTGSGPAPAPTCPSGPLAPGAQVRR588 pKa = 11.84CTATYY593 pKa = 7.36TTTQADD599 pKa = 3.56VDD601 pKa = 3.92RR602 pKa = 11.84GSIVNIATASATPPSGSPVVSDD624 pKa = 4.01PSTVTVTAAATPGITVVKK642 pKa = 8.82TATPQSVSAAGQTVDD657 pKa = 4.25YY658 pKa = 10.45AFQVTNTGNVTMNSVGITDD677 pKa = 3.84TEE679 pKa = 4.4FTGTGPAPEE688 pKa = 4.4VSCPSSTLAPGISMTCTASYY708 pKa = 8.5TATQADD714 pKa = 3.71IDD716 pKa = 4.18AGGFDD721 pKa = 3.82NTATATGTPPGSTPQTSPPSSTTVTAEE748 pKa = 3.94RR749 pKa = 11.84AASLALVKK757 pKa = 10.7SADD760 pKa = 3.66PSVVNAAGQTVDD772 pKa = 3.5YY773 pKa = 10.71SFLVTNTGNATVNDD787 pKa = 4.19LAVTEE792 pKa = 4.47TDD794 pKa = 3.95FSGSGPAPEE803 pKa = 4.84VSCPTDD809 pKa = 3.4PLAPGASTTCTASYY823 pKa = 10.79SATQADD829 pKa = 3.87IDD831 pKa = 4.4AGSITNTATAGGTDD845 pKa = 3.64PAGDD849 pKa = 4.08PVTSPPSSATVVAAVEE865 pKa = 4.26GDD867 pKa = 2.91ISLFKK872 pKa = 10.59SANPEE877 pKa = 4.07TVSAAGDD884 pKa = 3.4QVTFEE889 pKa = 4.18FLVTNVEE896 pKa = 4.12NVTLNAIRR904 pKa = 11.84VDD906 pKa = 3.64EE907 pKa = 4.44VEE909 pKa = 4.18FTGTGTLSPITCPPGPLAPGEE930 pKa = 4.1QRR932 pKa = 11.84TCTATYY938 pKa = 10.42AATQADD944 pKa = 4.1INAGSVTNTATASGTEE960 pKa = 3.9PDD962 pKa = 3.85GTDD965 pKa = 3.27VTSEE969 pKa = 3.95PASVVVTAEE978 pKa = 3.95AAPAMTVVKK987 pKa = 10.09SAQPQTVTAAGQTIDD1002 pKa = 3.85YY1003 pKa = 9.67SYY1005 pKa = 11.5QVTNTGNVTVDD1016 pKa = 3.39GVSVTEE1022 pKa = 4.0TDD1024 pKa = 3.77FTGTGPAPTPSCPDD1038 pKa = 3.12GPLAPGEE1045 pKa = 4.48STTCTASYY1053 pKa = 8.51TATQADD1059 pKa = 3.85IDD1061 pKa = 4.12AGGITNTATTTGTPPGGPPVTSPPSTSTVTTDD1093 pKa = 3.52PSPALTVAKK1102 pKa = 9.21TGEE1105 pKa = 4.17PATVSAAGQTITYY1118 pKa = 9.33SYY1120 pKa = 10.63EE1121 pKa = 3.6VRR1123 pKa = 11.84NTGNQTVTDD1132 pKa = 3.93VAVDD1136 pKa = 3.36EE1137 pKa = 4.94SGFTGTGPAPAATCPTTTLAPGEE1160 pKa = 4.54STTCTATYY1168 pKa = 9.46TVTQGDD1174 pKa = 3.57IDD1176 pKa = 4.05AGGVTNTGVATAQPPGGAPPVSSPPSTVTTGVEE1209 pKa = 4.27PNPSLAVVKK1218 pKa = 10.44SATPTHH1224 pKa = 5.88VARR1227 pKa = 11.84AGDD1230 pKa = 3.68VITYY1234 pKa = 9.98HH1235 pKa = 6.84FEE1237 pKa = 3.78ATNTGNVTLTGVRR1250 pKa = 11.84LDD1252 pKa = 3.65EE1253 pKa = 4.45AAFTGTGDD1261 pKa = 4.79LGPATCTGGPTLAPGEE1277 pKa = 4.14KK1278 pKa = 9.32RR1279 pKa = 11.84ICDD1282 pKa = 3.48LEE1284 pKa = 4.29YY1285 pKa = 10.87AVTQADD1291 pKa = 3.33IDD1293 pKa = 3.98AGSISNGATASGQPPSGARR1312 pKa = 11.84VTSPPAFATVTSDD1325 pKa = 3.42PAPAIMVDD1333 pKa = 3.41KK1334 pKa = 10.13TADD1337 pKa = 3.48PTTLTGAGQTVTYY1350 pKa = 10.29SFLVTNSGNVTLHH1363 pKa = 6.67DD1364 pKa = 3.51VGIRR1368 pKa = 11.84EE1369 pKa = 4.37TGFSGSGQLPPANCPTTSLEE1389 pKa = 4.18PGQQTTCTTSYY1400 pKa = 11.07AAAQTDD1406 pKa = 3.45VDD1408 pKa = 3.74QGFIANSAVAVGTGPGGAEE1427 pKa = 3.69AMSEE1431 pKa = 3.82QSAARR1436 pKa = 11.84VEE1438 pKa = 4.67IPPQPALSLVKK1449 pKa = 10.18TSDD1452 pKa = 3.23PTTASAAGDD1461 pKa = 3.44QVTFEE1466 pKa = 4.3YY1467 pKa = 10.9AVTNTGNVTLNGVGVGEE1484 pKa = 4.54TEE1486 pKa = 4.39FSGTGQPPVPTCPPEE1501 pKa = 4.26AAVLAPGATVTCTASYY1517 pKa = 9.83TLTQADD1523 pKa = 3.55VDD1525 pKa = 4.18AGGTTNTAVAGATPPGGPPAVISPPSTSTVTVDD1558 pKa = 3.71PGPGLSVVKK1567 pKa = 10.22SANPSSVDD1575 pKa = 3.27SAGDD1579 pKa = 3.38VVTYY1583 pKa = 9.82EE1584 pKa = 4.03FVVTNTGNVTMEE1596 pKa = 4.31DD1597 pKa = 3.42VSVAEE1602 pKa = 4.24TDD1604 pKa = 3.85FTGTGPAPVATCPPGPLAPGAQTTCTAPYY1633 pKa = 9.43TVTQADD1639 pKa = 3.34VDD1641 pKa = 3.9AGSISNTATATGEE1654 pKa = 4.26PPGSDD1659 pKa = 3.1TPIPPSPPSSTTVTIDD1675 pKa = 3.39PAPGLSVVKK1684 pKa = 10.53SVDD1687 pKa = 3.26PAGPEE1692 pKa = 3.99SFAVGQEE1699 pKa = 3.75LTYY1702 pKa = 10.9TFVATNTGNVTLHH1715 pKa = 6.93DD1716 pKa = 3.92VAIDD1720 pKa = 3.99DD1721 pKa = 4.48EE1722 pKa = 4.88DD1723 pKa = 4.91FSGSGGLGEE1732 pKa = 4.41PTCTGDD1738 pKa = 3.66PASVAPGEE1746 pKa = 4.38QIVCTATYY1754 pKa = 10.8VVTQADD1760 pKa = 3.3VDD1762 pKa = 3.85AGGVRR1767 pKa = 11.84NSATATGIPPAPGSPPVTSPPSQVEE1792 pKa = 4.64VPQDD1796 pKa = 3.73PQPGLSLVKK1805 pKa = 9.76TADD1808 pKa = 3.77TEE1810 pKa = 4.34HH1811 pKa = 6.5LTGPGQVITYY1821 pKa = 10.16SFLITNTGNVTLDD1834 pKa = 3.09RR1835 pKa = 11.84VAVDD1839 pKa = 3.19EE1840 pKa = 5.09GEE1842 pKa = 4.39FTGSGPLSPVTCPDD1856 pKa = 3.18EE1857 pKa = 5.22AGSLSPGEE1865 pKa = 4.15QATCTATYY1873 pKa = 9.45TVTQSDD1879 pKa = 3.65VDD1881 pKa = 3.87RR1882 pKa = 11.84GEE1884 pKa = 4.27LTNTATAGGTPPGGGGTTSPPSSATITGDD1913 pKa = 3.19AKK1915 pKa = 10.74PVLEE1919 pKa = 4.25VLKK1922 pKa = 9.21TAHH1925 pKa = 6.47WVDD1928 pKa = 4.12DD1929 pKa = 4.15NGSGVVDD1936 pKa = 4.64LGDD1939 pKa = 4.45RR1940 pKa = 11.84IAWTITATNTGGATATDD1957 pKa = 4.02VVVSDD1962 pKa = 4.06PTAGAVTCPATTLAPGEE1979 pKa = 4.64SMDD1982 pKa = 4.24CTAPDD1987 pKa = 3.69HH1988 pKa = 6.74VVDD1991 pKa = 4.37EE1992 pKa = 4.98ADD1994 pKa = 3.36VAAGEE1999 pKa = 4.34VANTAIATGRR2009 pKa = 11.84GPGGDD2014 pKa = 3.73PVTSPPASSTVEE2026 pKa = 3.82VGRR2029 pKa = 11.84PGTPPGPQPEE2039 pKa = 4.45EE2040 pKa = 3.8PGGPGDD2046 pKa = 3.98LATTGRR2052 pKa = 11.84DD2053 pKa = 3.22VGLLLALAGALLAFGGLLFSGARR2076 pKa = 11.84RR2077 pKa = 11.84RR2078 pKa = 11.84RR2079 pKa = 11.84RR2080 pKa = 11.84SS2081 pKa = 3.02

Molecular weight:
203.84 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1I6QSY8|A0A1I6QSY8_9PSEU PAS domain S-box-containing protein/diguanylate cyclase (GGDEF) domain-containing protein OS=Saccharopolyspora flava OX=95161 GN=SAMN05660874_01828 PE=4 SV=1
MM1 pKa = 7.27RR2 pKa = 11.84TLRR5 pKa = 11.84TGGALRR11 pKa = 11.84THH13 pKa = 5.08VTLRR17 pKa = 11.84AGGPLGSDD25 pKa = 3.49RR26 pKa = 11.84PLSTASPRR34 pKa = 11.84RR35 pKa = 11.84ALRR38 pKa = 11.84TGGPLGALSTLGALRR53 pKa = 11.84SRR55 pKa = 11.84RR56 pKa = 11.84SGQARR61 pKa = 11.84RR62 pKa = 11.84TLRR65 pKa = 11.84TGRR68 pKa = 11.84TLRR71 pKa = 11.84PRR73 pKa = 11.84GPLGARR79 pKa = 11.84SALSTRR85 pKa = 11.84SSLRR89 pKa = 11.84TSSALRR95 pKa = 11.84TRR97 pKa = 11.84CSLSTRR103 pKa = 11.84RR104 pKa = 11.84TLRR107 pKa = 11.84SGSALSTGRR116 pKa = 11.84TGSTLRR122 pKa = 11.84PRR124 pKa = 11.84RR125 pKa = 11.84TGRR128 pKa = 11.84TSRR131 pKa = 11.84ADD133 pKa = 3.3RR134 pKa = 11.84ALRR137 pKa = 11.84TRR139 pKa = 11.84GALSTGRR146 pKa = 11.84TRR148 pKa = 11.84STLRR152 pKa = 11.84TRR154 pKa = 11.84ITLRR158 pKa = 11.84PLSTRR163 pKa = 11.84STIRR167 pKa = 11.84TLRR170 pKa = 11.84ARR172 pKa = 11.84GTSRR176 pKa = 11.84TSGTDD181 pKa = 2.87LTLRR185 pKa = 11.84TRR187 pKa = 11.84SALRR191 pKa = 11.84TGLSLRR197 pKa = 11.84PRR199 pKa = 11.84RR200 pKa = 11.84TRR202 pKa = 11.84NAVSTLRR209 pKa = 11.84TRR211 pKa = 11.84RR212 pKa = 11.84ADD214 pKa = 3.12LTLRR218 pKa = 11.84TRR220 pKa = 11.84SALRR224 pKa = 11.84TRR226 pKa = 11.84RR227 pKa = 11.84TRR229 pKa = 11.84ITLRR233 pKa = 11.84PLRR236 pKa = 11.84TRR238 pKa = 11.84RR239 pKa = 11.84TGRR242 pKa = 11.84TSGTDD247 pKa = 2.96LTLRR251 pKa = 11.84TRR253 pKa = 11.84SALGTLRR260 pKa = 11.84TRR262 pKa = 11.84ISLRR266 pKa = 11.84ARR268 pKa = 11.84RR269 pKa = 11.84TRR271 pKa = 11.84STVSTLRR278 pKa = 11.84THH280 pKa = 7.21RR281 pKa = 11.84PDD283 pKa = 3.14LALRR287 pKa = 11.84PLRR290 pKa = 11.84TRR292 pKa = 11.84GAIGALRR299 pKa = 11.84THH301 pKa = 6.08RR302 pKa = 11.84TDD304 pKa = 2.88RR305 pKa = 11.84ALRR308 pKa = 11.84TRR310 pKa = 11.84RR311 pKa = 11.84TNITLRR317 pKa = 11.84PLRR320 pKa = 11.84SRR322 pKa = 11.84STVSSLRR329 pKa = 11.84TRR331 pKa = 11.84WADD334 pKa = 3.07LALRR338 pKa = 11.84TRR340 pKa = 11.84STGSALRR347 pKa = 11.84PRR349 pKa = 11.84GALSTIGTLRR359 pKa = 11.84THH361 pKa = 5.68RR362 pKa = 11.84TGRR365 pKa = 11.84PSRR368 pKa = 11.84TDD370 pKa = 2.95LALRR374 pKa = 11.84TRR376 pKa = 11.84SALRR380 pKa = 11.84TGRR383 pKa = 11.84ARR385 pKa = 11.84STLRR389 pKa = 11.84TRR391 pKa = 11.84ITLRR395 pKa = 11.84TLSTRR400 pKa = 11.84SAIGTLRR407 pKa = 11.84TRR409 pKa = 11.84RR410 pKa = 11.84TDD412 pKa = 3.02RR413 pKa = 11.84TSRR416 pKa = 11.84TDD418 pKa = 3.01LTLRR422 pKa = 11.84ARR424 pKa = 11.84STLRR428 pKa = 11.84TWGADD433 pKa = 3.06VALRR437 pKa = 11.84ALRR440 pKa = 11.84TGCAVGSGNALRR452 pKa = 11.84ADD454 pKa = 3.65GSLRR458 pKa = 11.84TGQPLWTGGALGTDD472 pKa = 3.41VALRR476 pKa = 11.84PLRR479 pKa = 11.84TGSARR484 pKa = 11.84RR485 pKa = 11.84TISALRR491 pKa = 11.84TNRR494 pKa = 11.84TRR496 pKa = 11.84RR497 pKa = 11.84ARR499 pKa = 11.84RR500 pKa = 11.84TDD502 pKa = 3.14LALRR506 pKa = 11.84SLNTGSARR514 pKa = 11.84GTVGTLRR521 pKa = 11.84TRR523 pKa = 11.84RR524 pKa = 11.84TIGALRR530 pKa = 11.84TGGTGRR536 pKa = 11.84ARR538 pKa = 11.84EE539 pKa = 4.06SLGTGVALRR548 pKa = 11.84PLRR551 pKa = 11.84ACGTRR556 pKa = 11.84RR557 pKa = 11.84AVGSLRR563 pKa = 11.84TGRR566 pKa = 11.84AHH568 pKa = 6.86RR569 pKa = 11.84AVGALRR575 pKa = 11.84ALRR578 pKa = 11.84SRR580 pKa = 11.84LARR583 pKa = 11.84ARR585 pKa = 11.84FSRR588 pKa = 11.84RR589 pKa = 11.84ALRR592 pKa = 11.84SFGSLGPGRR601 pKa = 11.84SGRR604 pKa = 11.84TRR606 pKa = 11.84DD607 pKa = 3.33AGSTGFALRR616 pKa = 11.84ANGPRR621 pKa = 11.84RR622 pKa = 11.84PGPALSTGGALRR634 pKa = 11.84AARR637 pKa = 11.84PSGWSLRR644 pKa = 11.84ALRR647 pKa = 11.84TVRR650 pKa = 11.84ALRR653 pKa = 11.84ALRR656 pKa = 11.84SFRR659 pKa = 11.84TGRR662 pKa = 11.84AGVRR666 pKa = 11.84SGRR669 pKa = 11.84QCRR672 pKa = 11.84RR673 pKa = 11.84GRR675 pKa = 11.84RR676 pKa = 11.84PRR678 pKa = 11.84LAEE681 pKa = 3.76HH682 pKa = 7.03RR683 pKa = 11.84SFHH686 pKa = 6.82PGRR689 pKa = 11.84THH691 pKa = 7.79AEE693 pKa = 3.75PGLFGTGDD701 pKa = 3.62GQRR704 pKa = 11.84GGRR707 pKa = 11.84SAQHH711 pKa = 6.34RR712 pKa = 11.84DD713 pKa = 3.12DD714 pKa = 4.1RR715 pKa = 11.84RR716 pKa = 11.84GGAHH720 pKa = 7.47DD721 pKa = 5.06DD722 pKa = 3.3VDD724 pKa = 3.85GAARR728 pKa = 11.84SSRR731 pKa = 11.84PATTHH736 pKa = 6.28EE737 pKa = 4.42SPPTILIVPLSVSCLDD753 pKa = 3.64SPIIGEE759 pKa = 4.38KK760 pKa = 8.92TSVWNPPFLNEE771 pKa = 4.05KK772 pKa = 10.2

Molecular weight:
84.32 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5595

0

5595

1855314

39

10081

331.6

35.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.732 ± 0.051

0.787 ± 0.01

6.223 ± 0.029

6.236 ± 0.032

2.933 ± 0.02

9.195 ± 0.037

2.316 ± 0.017

3.511 ± 0.024

2.016 ± 0.028

10.379 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.839 ± 0.016

1.936 ± 0.018

5.928 ± 0.034

3.023 ± 0.023

7.819 ± 0.04

5.283 ± 0.032

5.788 ± 0.032

8.723 ± 0.034

1.467 ± 0.013

1.866 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski