Pandoravirus quercus
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U7UB16|A0A2U7UB16_9VIRU Uncharacterized protein OS=Pandoravirus quercus OX=2107709 GN=pqer_cds_1170 PE=4 SV=1
MM1 pKa = 7.67 ARR3 pKa = 11.84 PTTDD7 pKa = 2.13 AWVYY11 pKa = 10.62 EE12 pKa = 4.5 DD13 pKa = 4.69 GWPNDD18 pKa = 3.53 DD19 pKa = 5.68 DD20 pKa = 3.93 EE21 pKa = 5.42 TYY23 pKa = 10.77 QCSDD27 pKa = 4.9 DD28 pKa = 3.93 NNQLAGWGGLHH39 pKa = 6.64 GWDD42 pKa = 3.34 DD43 pKa = 4.53 CIYY46 pKa = 10.95 NDD48 pKa = 3.06 ILAYY52 pKa = 7.16 QTRR55 pKa = 11.84 DD56 pKa = 3.31 DD57 pKa = 3.83 TVYY60 pKa = 11.03 NAIGSCIDD68 pKa = 4.11 HH69 pKa = 6.78 NNADD73 pKa = 4.1 DD74 pKa = 4.23 GTWGNGDD81 pKa = 4.42 DD82 pKa = 4.44 DD83 pKa = 4.69 VSGLGEE89 pKa = 4.3 CPVAPKK95 pKa = 8.61 HH96 pKa = 5.17 TPYY99 pKa = 10.9 AYY101 pKa = 9.97 GYY103 pKa = 7.53 TFIEE107 pKa = 4.79 GFMPADD113 pKa = 3.45 SFDD116 pKa = 3.74 ALVRR120 pKa = 11.84 PEE122 pKa = 3.77 RR123 pKa = 11.84 RR124 pKa = 11.84 LVVVVGGVPVALDD137 pKa = 3.27 AAEE140 pKa = 4.13 VEE142 pKa = 4.49 RR143 pKa = 11.84 AGNVGRR149 pKa = 11.84 PLATPLGSCSLGPLDD164 pKa = 4.57 VEE166 pKa = 4.62 RR167 pKa = 11.84 AHH169 pKa = 6.64 EE170 pKa = 4.25 ADD172 pKa = 3.09 EE173 pKa = 4.16 ALYY176 pKa = 10.4 EE177 pKa = 4.15 RR178 pKa = 11.84 EE179 pKa = 3.78 GLRR182 pKa = 11.84 RR183 pKa = 11.84 FLTTAPVTATIEE195 pKa = 3.94 AAAPALGAGAGAAADD210 pKa = 3.52 IAGGSILAGVLGGLFAALLL229 pKa = 4.05
Molecular weight: 24.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.706
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.617
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.279
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.05
Patrickios 1.214
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.932
Protein with the highest isoelectric point:
>tr|A0A2U7U9V9|A0A2U7U9V9_9VIRU Uncharacterized protein OS=Pandoravirus quercus OX=2107709 GN=pqer_cds_794 PE=4 SV=1
MM1 pKa = 7.71 DD2 pKa = 5.2 TLPAEE7 pKa = 4.63 IGCMILGYY15 pKa = 10.28 LEE17 pKa = 4.38 PTWLASVSMASRR29 pKa = 11.84 GLYY32 pKa = 9.1 HH33 pKa = 7.17 WGALVCRR40 pKa = 11.84 GEE42 pKa = 4.27 ALRR45 pKa = 11.84 MPRR48 pKa = 11.84 NAMEE52 pKa = 3.79 VAAANGAWDD61 pKa = 4.16 FMQWLRR67 pKa = 11.84 HH68 pKa = 5.03 HH69 pKa = 7.32 LYY71 pKa = 10.01 WPWTPVTLATAAVHH85 pKa = 5.24 NQRR88 pKa = 11.84 LVFKK92 pKa = 10.22 RR93 pKa = 11.84 LFKK96 pKa = 11.05 GRR98 pKa = 11.84 TCSVDD103 pKa = 2.95 ARR105 pKa = 11.84 AAAAALVGGGPALLCTALRR124 pKa = 11.84 HH125 pKa = 5.49 GCPKK129 pKa = 10.47 SGLLTTVACLLGRR142 pKa = 11.84 DD143 pKa = 4.01 GLASRR148 pKa = 11.84 LMRR151 pKa = 11.84 TTVPDD156 pKa = 4.36 DD157 pKa = 3.78 LMNLCGLATRR167 pKa = 11.84 NWQILSFMTPGDD179 pKa = 3.22 WRR181 pKa = 11.84 RR182 pKa = 11.84 TRR184 pKa = 11.84 RR185 pKa = 11.84 WLSDD189 pKa = 3.49 PDD191 pKa = 3.47 RR192 pKa = 11.84 APIDD196 pKa = 3.66 LRR198 pKa = 11.84 VAAHH202 pKa = 5.83 QLAEE206 pKa = 4.38 HH207 pKa = 7.26 RR208 pKa = 11.84 DD209 pKa = 3.87 TLAGLAKK216 pKa = 10.22 KK217 pKa = 10.09 LALGEE222 pKa = 4.07 IARR225 pKa = 11.84 RR226 pKa = 11.84 PPPPPPPRR234 pKa = 11.84 LPLGAGYY241 pKa = 9.38 PDD243 pKa = 3.48 WLHH246 pKa = 6.0 YY247 pKa = 10.16 CIDD250 pKa = 3.87 MIRR253 pKa = 11.84 WKK255 pKa = 10.43 NDD257 pKa = 2.78 CLFRR261 pKa = 11.84 EE262 pKa = 4.11 RR263 pKa = 11.84 RR264 pKa = 11.84 QWHH267 pKa = 5.91 EE268 pKa = 4.11 EE269 pKa = 4.05 SPQRR273 pKa = 11.84 WPKK276 pKa = 10.01 PVLADD281 pKa = 4.35 RR282 pKa = 11.84 SPGPEE287 pKa = 4.14 SKK289 pKa = 10.58 CRR291 pKa = 11.84 TPVEE295 pKa = 3.77 KK296 pKa = 10.5 RR297 pKa = 11.84 QPHH300 pKa = 6.24 TIARR304 pKa = 11.84 MQPRR308 pKa = 11.84 RR309 pKa = 11.84 HH310 pKa = 6.01 ARR312 pKa = 11.84 RR313 pKa = 11.84 PWRR316 pKa = 3.84
Molecular weight: 35.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 9.399
IPC_protein 10.189
Toseland 10.467
ProMoST 10.335
Dawson 10.584
Bjellqvist 10.335
Wikipedia 10.804
Rodwell 10.672
Grimsley 10.628
Solomon 10.701
Lehninger 10.672
Nozaki 10.526
DTASelect 10.306
Thurlkill 10.482
EMBOSS 10.877
Sillero 10.526
Patrickios 10.35
IPC_peptide 10.701
IPC2_peptide 9.75
IPC2.peptide.svr19 8.569
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1179
0
1179
468644
57
2278
397.5
42.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.946 ± 0.082
2.536 ± 0.048
7.561 ± 0.078
3.831 ± 0.051
2.22 ± 0.027
7.937 ± 0.078
3.01 ± 0.05
3.223 ± 0.031
2.01 ± 0.048
8.122 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.079 ± 0.025
2.241 ± 0.039
6.645 ± 0.06
2.74 ± 0.043
8.472 ± 0.068
5.842 ± 0.063
6.009 ± 0.059
7.388 ± 0.051
2.029 ± 0.037
2.16 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here