Mariniflexile sp. TRM1-10

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mariniflexile; unclassified Mariniflexile

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4002 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385BTU7|A0A385BTU7_9FLAO Penicillin-binding protein 4 OS=Mariniflexile sp. TRM1-10 OX=2027857 GN=CJ739_3516 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 9.74TFKK5 pKa = 10.15PLSIFTLIMLVLFAACEE22 pKa = 3.78KK23 pKa = 11.0DD24 pKa = 5.12DD25 pKa = 4.66FIEE28 pKa = 4.77TIGICPLVIATNPEE42 pKa = 3.51NDD44 pKa = 3.5ATNVPLSQVITVTFNVEE61 pKa = 4.09MNPTTITEE69 pKa = 3.78ASFIIEE75 pKa = 3.89SGAAITGTVTYY86 pKa = 10.69NGFTATFTPAAPLEE100 pKa = 4.22ISTTYY105 pKa = 9.17TGRR108 pKa = 11.84IKK110 pKa = 10.17TSVKK114 pKa = 10.42DD115 pKa = 3.77LNGNALQEE123 pKa = 4.27EE124 pKa = 5.33YY125 pKa = 11.01VWTFSTGATVSPMVISTNPEE145 pKa = 3.82DD146 pKa = 3.99NEE148 pKa = 4.12TDD150 pKa = 3.71VVINTIITATFSQQMDD166 pKa = 3.98PLTINEE172 pKa = 4.24TSFILMDD179 pKa = 4.4GSTAITGVVTYY190 pKa = 10.96NDD192 pKa = 3.15VTATFTPSANLDD204 pKa = 3.57ANTLYY209 pKa = 10.91SVTITTDD216 pKa = 2.75ATNTTGISLEE226 pKa = 4.18NNYY229 pKa = 9.35EE230 pKa = 3.86WSFTTGTLISPMVISTDD247 pKa = 3.6PEE249 pKa = 4.3DD250 pKa = 4.32NEE252 pKa = 4.72IDD254 pKa = 3.68VALDD258 pKa = 3.23KK259 pKa = 11.03TITATFSEE267 pKa = 4.52PMDD270 pKa = 4.18LATIDD275 pKa = 3.65EE276 pKa = 4.76TTFTLQEE283 pKa = 3.92GTNFITGTISYY294 pKa = 9.46NALTASFVPSVDD306 pKa = 3.95LLPGTLYY313 pKa = 10.86KK314 pKa = 10.27ATITTGATNVAGIPLANNYY333 pKa = 7.44EE334 pKa = 4.27WEE336 pKa = 4.37FTTAGTPFEE345 pKa = 4.1PTIDD349 pKa = 3.55LGSVEE354 pKa = 4.34RR355 pKa = 11.84FGIISGVAVSNNAGPSEE372 pKa = 3.87IHH374 pKa = 7.06DD375 pKa = 4.69LDD377 pKa = 4.27IGIYY381 pKa = 9.28PGARR385 pKa = 11.84SSITGFFDD393 pKa = 3.29VDD395 pKa = 3.95GGPGLIFNGDD405 pKa = 3.57FYY407 pKa = 11.52AADD410 pKa = 4.25DD411 pKa = 4.65ADD413 pKa = 4.51PVPAMLLQAKK423 pKa = 9.97DD424 pKa = 4.19DD425 pKa = 3.73LTAAYY430 pKa = 9.36LAAEE434 pKa = 4.97GATSPAPATVAGDD447 pKa = 3.93LGGQTLPPGIYY458 pKa = 9.8KK459 pKa = 10.71SNSTLMIQNGNLTLDD474 pKa = 3.85GQGDD478 pKa = 4.11PNAEE482 pKa = 4.25WIFQIASDD490 pKa = 4.16FTTVGGSPYY499 pKa = 9.89PSPAGGNVILIGGAQAKK516 pKa = 9.95NIIWQVGSSAVIGDD530 pKa = 3.59YY531 pKa = 11.03TSFKK535 pKa = 11.07GNVLALTSVTMNAYY549 pKa = 8.54SQAVGRR555 pKa = 11.84MLCSNGAVTLTSTNFIYY572 pKa = 10.6RR573 pKa = 11.84PP574 pKa = 3.49

Molecular weight: 60.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385BK77|A0A385BK77_9FLAO Uncharacterized protein OS=Mariniflexile sp. TRM1-10 OX=2027857 GN=CJ739_386 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.6LSVSSEE48 pKa = 4.04SRR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.69KK53 pKa = 9.55

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4002

0

4002

1401172

29

4580

350.1

39.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.167 ± 0.039

0.767 ± 0.016

5.611 ± 0.028

6.336 ± 0.041

5.2 ± 0.031

6.363 ± 0.038

1.831 ± 0.016

8.086 ± 0.038

7.836 ± 0.05

9.107 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.019

6.584 ± 0.038

3.384 ± 0.024

3.262 ± 0.019

3.205 ± 0.024

6.58 ± 0.031

6.073 ± 0.052

6.086 ± 0.032

1.132 ± 0.016

4.273 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski