Mesorhizobium sp. 8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium; unclassified Mesorhizobium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4486 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8B120|A0A5B8B120_9RHIZ Uncharacterized protein OS=Mesorhizobium sp. 8 OX=2584466 GN=FGU64_07890 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84DD3 pKa = 3.42DD4 pKa = 3.76YY5 pKa = 12.01LEE7 pKa = 4.51FKK9 pKa = 10.61ALQATSEE16 pKa = 4.05PTQADD21 pKa = 3.3DD22 pKa = 3.73HH23 pKa = 6.56AQLSFQSPYY32 pKa = 11.41DD33 pKa = 3.82FDD35 pKa = 6.59DD36 pKa = 5.0PDD38 pKa = 5.22PGIHH42 pKa = 5.79YY43 pKa = 10.33VPAGPTIEE51 pKa = 4.28TMTVPGGVAFTPMEE65 pKa = 4.33IPVVPAANPALYY77 pKa = 10.1VPSEE81 pKa = 4.34SYY83 pKa = 10.54PDD85 pKa = 4.48FISRR89 pKa = 11.84HH90 pKa = 4.09FTFAHH95 pKa = 5.88SSGSIQLQPPGSVATHH111 pKa = 5.16VVQINTLSDD120 pKa = 3.28NDD122 pKa = 3.88FVNVGAGNVTFHH134 pKa = 7.49AIGMPNLDD142 pKa = 4.92LDD144 pKa = 4.9ALYY147 pKa = 10.72QDD149 pKa = 4.93ALQFLPIPPASFSSTEE165 pKa = 4.27DD166 pKa = 2.44IGSFVNEE173 pKa = 3.8TAASLKK179 pKa = 10.78AFVAGVEE186 pKa = 4.22AAGQGTPDD194 pKa = 3.53GGAEE198 pKa = 3.94MSVLTDD204 pKa = 3.64DD205 pKa = 5.14GGVSVQTHH213 pKa = 5.14VSVFDD218 pKa = 4.01APIADD223 pKa = 4.94AIYY226 pKa = 10.16VGGRR230 pKa = 11.84IAAEE234 pKa = 3.95APKK237 pKa = 10.54LDD239 pKa = 4.3DD240 pKa = 4.03VLPAASPLVKK250 pKa = 10.6DD251 pKa = 4.07PVQAPSSADD260 pKa = 3.36PAGGNHH266 pKa = 7.47LIDD269 pKa = 4.53GSGSTATGVVIHH281 pKa = 6.03GTGNVAGEE289 pKa = 4.35TSVSLNTGSNALVNSATIVNDD310 pKa = 3.6ALEE313 pKa = 4.55GSVFAVAGNHH323 pKa = 5.24YY324 pKa = 10.01ALNAIVQVNAWSDD337 pKa = 3.23SDD339 pKa = 4.66SIGGSLSGWNAQQQPDD355 pKa = 3.64TTAFNIAQMLNIDD368 pKa = 3.66TGEE371 pKa = 4.07NAGSGNSGQPSFPKK385 pKa = 9.8AWAVTEE391 pKa = 4.05INGDD395 pKa = 4.31FISLNWTHH403 pKa = 5.89QVNFLLDD410 pKa = 3.28NDD412 pKa = 4.46TAVVSSTSGVTTTVGTGTNQQTNALSIADD441 pKa = 3.75LGKK444 pKa = 10.98YY445 pKa = 9.59YY446 pKa = 10.83DD447 pKa = 3.99LVLIGGSYY455 pKa = 10.67YY456 pKa = 10.57DD457 pKa = 4.44ANIITQTNVLLDD469 pKa = 3.73NDD471 pKa = 4.16VVGALANFQTQGQGSVSNGDD491 pKa = 3.25NLLWNNAKK499 pKa = 8.46ITSIGSSANAALPIGFAKK517 pKa = 10.22TLDD520 pKa = 4.0DD521 pKa = 4.22FAHH524 pKa = 6.61GNHH527 pKa = 6.3TLSSDD532 pKa = 3.83VLNDD536 pKa = 3.2EE537 pKa = 4.83AFQGLGGLKK546 pKa = 9.84VLYY549 pKa = 10.12ISGSVYY555 pKa = 10.35DD556 pKa = 4.36LQYY559 pKa = 10.92ISQTNVLGDD568 pKa = 3.74SDD570 pKa = 5.25DD571 pKa = 3.64VALALNAAHH580 pKa = 7.3ANTDD584 pKa = 3.98ANWSIVTGSNALINSAQIVDD604 pKa = 3.82VDD606 pKa = 4.32PAAKK610 pKa = 9.32TYY612 pKa = 11.08VGGDD616 pKa = 3.38HH617 pKa = 6.96YY618 pKa = 11.48SDD620 pKa = 3.96EE621 pKa = 5.16LLVQTDD627 pKa = 4.58IISTDD632 pKa = 3.44HH633 pKa = 7.32LLQTQDD639 pKa = 3.1ADD641 pKa = 3.79HH642 pKa = 6.69LVNEE646 pKa = 4.72AVAFLSDD653 pKa = 4.59DD654 pKa = 3.56MTGPAHH660 pKa = 6.76EE661 pKa = 5.06NPASQLQAPLDD672 pKa = 3.7VHH674 pKa = 6.59PAHH677 pKa = 6.88SDD679 pKa = 3.08VMQSVISS686 pKa = 3.74

Molecular weight: 71.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8B6G4|A0A5B8B6G4_9RHIZ ABC transporter ATP-binding protein OS=Mesorhizobium sp. 8 OX=2584466 GN=FGU64_16800 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25GGRR28 pKa = 11.84GVIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4486

0

4486

1410974

17

11922

314.5

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.929 ± 0.05

0.78 ± 0.012

5.752 ± 0.038

5.643 ± 0.041

3.852 ± 0.024

8.869 ± 0.06

1.961 ± 0.02

5.103 ± 0.029

3.607 ± 0.037

9.848 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.026

2.565 ± 0.028

5.046 ± 0.036

2.877 ± 0.018

6.892 ± 0.053

5.261 ± 0.039

5.276 ± 0.053

7.661 ± 0.029

1.304 ± 0.016

2.259 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski