Mycobacterium phage Spud

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bixzunavirus; Mycobacterium virus Bxz1

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5LKP0|B5LKP0_9CAUD Minor tail protein OS=Mycobacterium phage Spud OX=546808 GN=135 PE=3 SV=1
MM1 pKa = 7.81ADD3 pKa = 3.58YY4 pKa = 11.13ASDD7 pKa = 4.1CPISRR12 pKa = 11.84PVDD15 pKa = 3.83PGWTSDD21 pKa = 2.87WDD23 pKa = 3.89LGGMQWSYY31 pKa = 11.65DD32 pKa = 3.36YY33 pKa = 11.9GMFYY37 pKa = 10.44QPSPDD42 pKa = 3.22EE43 pKa = 4.38LLPLRR48 pKa = 11.84KK49 pKa = 8.64PAQRR53 pKa = 11.84VGEE56 pKa = 4.11

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5LKU4|B5LKU4_9CAUD Uncharacterized protein OS=Mycobacterium phage Spud OX=546808 GN=219 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.08TALRR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84FHH12 pKa = 6.69HH13 pKa = 7.25LSGLQQTQIYY23 pKa = 9.87RR24 pKa = 11.84EE25 pKa = 3.9IEE27 pKa = 4.06KK28 pKa = 10.6AIEE31 pKa = 4.19PYY33 pKa = 10.19RR34 pKa = 11.84GLRR37 pKa = 11.84GLTARR42 pKa = 11.84TKK44 pKa = 8.62EE45 pKa = 4.31TMSVRR50 pKa = 11.84LARR53 pKa = 11.84KK54 pKa = 8.85FGVPRR59 pKa = 11.84KK60 pKa = 10.1VILDD64 pKa = 3.6VYY66 pKa = 9.38EE67 pKa = 3.74YY68 pKa = 10.65RR69 pKa = 11.84AAQRR73 pKa = 11.84HH74 pKa = 4.65EE75 pKa = 4.21RR76 pKa = 11.84EE77 pKa = 4.09

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

222

0

222

47025

31

1369

211.8

23.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.748 ± 0.234

0.855 ± 0.073

7.103 ± 0.176

6.446 ± 0.196

3.105 ± 0.096

8.045 ± 0.263

2.465 ± 0.119

4.279 ± 0.131

3.779 ± 0.123

7.617 ± 0.224

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.102

3.407 ± 0.105

5.686 ± 0.166

4.164 ± 0.118

6.845 ± 0.177

5.423 ± 0.162

6.431 ± 0.18

7.158 ± 0.194

1.89 ± 0.08

3.045 ± 0.086
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski