delta proteobacterium MLMS-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; unclassified Deltaproteobacteria

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1NSR5|Q1NSR5_9DELT Uncharacterized protein OS=delta proteobacterium MLMS-1 OX=262489 GN=MldDRAFT_1382 PE=4 SV=1
MM1 pKa = 6.6YY2 pKa = 10.16QSRR5 pKa = 11.84HH6 pKa = 4.83PQSFAVLLVAFIYY19 pKa = 10.55LFTAAFWVPPVAAAEE34 pKa = 4.01ATEE37 pKa = 3.93AAKK40 pKa = 10.2IVALRR45 pKa = 11.84GSAVAIDD52 pKa = 3.66ADD54 pKa = 4.15GVEE57 pKa = 4.43RR58 pKa = 11.84TLAPGDD64 pKa = 3.86SLAVSDD70 pKa = 5.02TIRR73 pKa = 11.84TGPRR77 pKa = 11.84GRR79 pKa = 11.84LQLMFSDD86 pKa = 3.69NTIISLGVNAEE97 pKa = 4.33LEE99 pKa = 4.06ISSYY103 pKa = 11.14HH104 pKa = 7.3LDD106 pKa = 3.58EE107 pKa = 6.29DD108 pKa = 3.94SGEE111 pKa = 4.21LATTVNRR118 pKa = 11.84GAFRR122 pKa = 11.84VMGGSIARR130 pKa = 11.84TSPEE134 pKa = 4.05SFTTDD139 pKa = 3.1TPSATIGIRR148 pKa = 11.84GSMYY152 pKa = 10.52AGRR155 pKa = 11.84VSNGALTVVFQGGVGITVSNPAGTVEE181 pKa = 3.81ITTPGMGTRR190 pKa = 11.84VRR192 pKa = 11.84SRR194 pKa = 11.84DD195 pKa = 3.5EE196 pKa = 4.17APEE199 pKa = 3.85EE200 pKa = 3.82PRR202 pKa = 11.84PFGEE206 pKa = 5.4DD207 pKa = 3.04DD208 pKa = 3.59LAEE211 pKa = 4.62LEE213 pKa = 4.58DD214 pKa = 3.97EE215 pKa = 4.5EE216 pKa = 4.67EE217 pKa = 4.04ATEE220 pKa = 4.14EE221 pKa = 4.05EE222 pKa = 4.47EE223 pKa = 4.29EE224 pKa = 4.21ATEE227 pKa = 4.45EE228 pKa = 4.16EE229 pKa = 4.23ATEE232 pKa = 4.2DD233 pKa = 3.91DD234 pKa = 4.15EE235 pKa = 5.52AAEE238 pKa = 4.05EE239 pKa = 4.18QPADD243 pKa = 3.62EE244 pKa = 4.84EE245 pKa = 4.43EE246 pKa = 4.24WDD248 pKa = 4.03EE249 pKa = 4.19EE250 pKa = 4.31ALDD253 pKa = 4.16EE254 pKa = 5.8ADD256 pKa = 3.45TTTDD260 pKa = 3.11TTEE263 pKa = 4.0TTTTTEE269 pKa = 4.17VEE271 pKa = 4.46DD272 pKa = 4.01VATEE276 pKa = 4.36ASQDD280 pKa = 3.65EE281 pKa = 4.38QQDD284 pKa = 3.78DD285 pKa = 4.04VTQKK289 pKa = 11.09AEE291 pKa = 4.01EE292 pKa = 4.15TATEE296 pKa = 3.83EE297 pKa = 4.49DD298 pKa = 3.61ATHH301 pKa = 6.65SGRR304 pKa = 11.84AIWVWQEE311 pKa = 3.64EE312 pKa = 4.75GEE314 pKa = 4.19EE315 pKa = 4.52AEE317 pKa = 4.22VQLISFEE324 pKa = 4.52DD325 pKa = 3.37HH326 pKa = 6.23WSEE329 pKa = 5.23HH330 pKa = 7.21DD331 pKa = 5.02DD332 pKa = 4.32GHH334 pKa = 7.72DD335 pKa = 4.29DD336 pKa = 4.7LPKK339 pKa = 9.91DD340 pKa = 4.03TYY342 pKa = 10.74TGPHH346 pKa = 6.23WDD348 pKa = 3.93EE349 pKa = 4.82ASGDD353 pKa = 3.77DD354 pKa = 3.2WWWRR358 pKa = 11.84GGYY361 pKa = 8.02EE362 pKa = 4.18DD363 pKa = 5.33RR364 pKa = 11.84HH365 pKa = 5.32EE366 pKa = 4.58FWVKK370 pKa = 8.72MDD372 pKa = 3.78NYY374 pKa = 10.43DD375 pKa = 3.32GHH377 pKa = 7.66SEE379 pKa = 4.67GNLQDD384 pKa = 3.09SWFSFYY390 pKa = 11.29GLEE393 pKa = 4.23TTTGQMPGSGGSQYY407 pKa = 11.29SGYY410 pKa = 10.98FMGADD415 pKa = 3.89HH416 pKa = 7.53DD417 pKa = 4.35PSYY420 pKa = 10.63PNPDD424 pKa = 3.08FGEE427 pKa = 4.33FQFVTNWRR435 pKa = 11.84TGKK438 pKa = 10.18AVGYY442 pKa = 9.91FEE444 pKa = 5.79EE445 pKa = 6.85GDD447 pKa = 3.48DD448 pKa = 3.53TRR450 pKa = 11.84VFFHH454 pKa = 7.38ADD456 pKa = 2.99VSGTTLSNVQIMGTHH471 pKa = 6.08YY472 pKa = 10.96YY473 pKa = 10.83DD474 pKa = 4.72DD475 pKa = 4.22GEE477 pKa = 4.02EE478 pKa = 4.17RR479 pKa = 11.84YY480 pKa = 9.97EE481 pKa = 4.09YY482 pKa = 11.12VAGDD486 pKa = 3.65SNFFSFYY493 pKa = 9.66GAYY496 pKa = 9.42YY497 pKa = 10.04QGFGMTTQGEE507 pKa = 4.55NYY509 pKa = 9.82SLEE512 pKa = 4.04NMEE515 pKa = 6.24SVVQWSAISAGFRR528 pKa = 11.84EE529 pKa = 3.99TDD531 pKa = 3.22DD532 pKa = 3.56NYY534 pKa = 11.47SYY536 pKa = 11.87VDD538 pKa = 3.51NTATSHH544 pKa = 4.56WQGFVTGAAINVAGTDD560 pKa = 3.79DD561 pKa = 4.16NNLLFMNDD569 pKa = 3.59DD570 pKa = 3.69PEE572 pKa = 5.18AFNFLINRR580 pKa = 11.84EE581 pKa = 3.82QGMFSGEE588 pKa = 3.9INISNIFTDD597 pKa = 3.1TDD599 pKa = 3.22GDD601 pKa = 4.12YY602 pKa = 11.17EE603 pKa = 4.86LNLGAQGTYY612 pKa = 10.24VADD615 pKa = 3.58NFMLGNIDD623 pKa = 3.64GTVTNHH629 pKa = 5.97DD630 pKa = 4.1TEE632 pKa = 4.72TSGSLNQYY640 pKa = 10.4GNMIVASPEE649 pKa = 3.43ISMDD653 pKa = 3.32EE654 pKa = 4.4HH655 pKa = 8.3ISWGYY660 pKa = 9.32WSVSFDD666 pKa = 4.38DD667 pKa = 3.96PHH669 pKa = 6.46STDD672 pKa = 2.51TTYY675 pKa = 11.82ALDD678 pKa = 3.66PRR680 pKa = 11.84ASFWVAGEE688 pKa = 4.21STPAEE693 pKa = 4.21YY694 pKa = 11.04VEE696 pKa = 4.64GLIDD700 pKa = 3.69NDD702 pKa = 3.86YY703 pKa = 10.54VGQYY707 pKa = 9.52TGGAQGIKK715 pKa = 10.29VEE717 pKa = 4.19NDD719 pKa = 3.05VIAALTNGVTDD730 pKa = 3.34LTIYY734 pKa = 10.5FGSGTMAGSISFDD747 pKa = 3.25EE748 pKa = 4.31VSLNVDD754 pKa = 3.42SGSVSSSGFSADD766 pKa = 3.43FSNMNSSGLKK776 pKa = 10.16GGFFGDD782 pKa = 3.6QAQSIGGNFQAEE794 pKa = 4.66DD795 pKa = 3.58GDD797 pKa = 4.07ADD799 pKa = 3.73YY800 pKa = 11.63SGVFGADD807 pKa = 2.59MEE809 pKa = 4.55MPHH812 pKa = 6.58

Molecular weight: 88.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1NM73|Q1NM73_9DELT Pseudouridine synthase OS=delta proteobacterium MLMS-1 OX=262489 GN=MldDRAFT_3455 PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.22QPSNTKK12 pKa = 8.42RR13 pKa = 11.84HH14 pKa = 5.05RR15 pKa = 11.84THH17 pKa = 6.71GFRR20 pKa = 11.84VRR22 pKa = 11.84MSTKK26 pKa = 10.09NGRR29 pKa = 11.84AVINRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 8.8RR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4800

0

4800

1571437

32

3532

327.4

36.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.751 ± 0.05

1.14 ± 0.016

5.269 ± 0.031

6.919 ± 0.038

3.589 ± 0.019

7.92 ± 0.034

2.306 ± 0.016

5.128 ± 0.028

3.619 ± 0.028

11.601 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.154 ± 0.016

2.897 ± 0.024

5.186 ± 0.032

4.274 ± 0.027

7.4 ± 0.042

4.91 ± 0.024

4.688 ± 0.039

6.553 ± 0.027

1.155 ± 0.014

2.538 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski