Amino acid dipepetide frequency for Synechococcus phage S-RIM2 R1_1999

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.632AlaAla: 6.632 ± 0.451
0.464AlaCys: 0.464 ± 0.12
4.261AlaAsp: 4.261 ± 0.286
4.029AlaGlu: 4.029 ± 0.316
3.174AlaPhe: 3.174 ± 0.282
6.722AlaGly: 6.722 ± 0.483
0.784AlaHis: 0.784 ± 0.117
4.261AlaIle: 4.261 ± 0.273
4.226AlaLys: 4.226 ± 0.398
4.796AlaLeu: 4.796 ± 0.331
1.48AlaMet: 1.48 ± 0.223
4.136AlaAsn: 4.136 ± 0.432
2.71AlaPro: 2.71 ± 0.227
2.657AlaGln: 2.657 ± 0.179
2.888AlaArg: 2.888 ± 0.24
5.028AlaSer: 5.028 ± 0.398
5.955AlaThr: 5.955 ± 0.569
4.956AlaVal: 4.956 ± 0.402
0.749AlaTrp: 0.749 ± 0.147
2.371AlaTyr: 2.371 ± 0.203
0.0AlaXaa: 0.0 ± 0.0
Cys
0.571CysAla: 0.571 ± 0.118
0.036CysCys: 0.036 ± 0.025
0.678CysAsp: 0.678 ± 0.133
0.517CysGlu: 0.517 ± 0.119
0.41CysPhe: 0.41 ± 0.098
0.678CysGly: 0.678 ± 0.131
0.214CysHis: 0.214 ± 0.065
0.499CysIle: 0.499 ± 0.115
0.731CysLys: 0.731 ± 0.136
0.66CysLeu: 0.66 ± 0.14
0.25CysMet: 0.25 ± 0.068
0.642CysAsn: 0.642 ± 0.143
0.321CysPro: 0.321 ± 0.084
0.428CysGln: 0.428 ± 0.083
0.357CysArg: 0.357 ± 0.087
0.588CysSer: 0.588 ± 0.124
0.481CysThr: 0.481 ± 0.095
0.606CysVal: 0.606 ± 0.121
0.125CysTrp: 0.125 ± 0.055
0.374CysTyr: 0.374 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
5.67AspAla: 5.67 ± 0.347
0.802AspCys: 0.802 ± 0.147
4.332AspAsp: 4.332 ± 0.274
3.762AspGlu: 3.762 ± 0.35
3.031AspPhe: 3.031 ± 0.197
5.866AspGly: 5.866 ± 0.498
0.927AspHis: 0.927 ± 0.166
4.315AspIle: 4.315 ± 0.341
3.53AspLys: 3.53 ± 0.304
4.511AspLeu: 4.511 ± 0.285
1.515AspMet: 1.515 ± 0.214
3.423AspAsn: 3.423 ± 0.265
3.298AspPro: 3.298 ± 0.272
2.139AspGln: 2.139 ± 0.187
2.692AspArg: 2.692 ± 0.211
4.261AspSer: 4.261 ± 0.326
4.439AspThr: 4.439 ± 0.338
4.368AspVal: 4.368 ± 0.295
1.088AspTrp: 1.088 ± 0.149
2.728AspTyr: 2.728 ± 0.207
0.0AspXaa: 0.0 ± 0.0
Glu
3.405GluAla: 3.405 ± 0.304
0.874GluCys: 0.874 ± 0.16
4.136GluAsp: 4.136 ± 0.283
4.832GluGlu: 4.832 ± 0.575
3.174GluPhe: 3.174 ± 0.227
4.083GluGly: 4.083 ± 0.286
1.016GluHis: 1.016 ± 0.182
3.637GluIle: 3.637 ± 0.293
3.744GluLys: 3.744 ± 0.461
5.099GluLeu: 5.099 ± 0.299
1.551GluMet: 1.551 ± 0.255
3.245GluAsn: 3.245 ± 0.251
1.587GluPro: 1.587 ± 0.187
2.407GluGln: 2.407 ± 0.215
2.977GluArg: 2.977 ± 0.371
3.637GluSer: 3.637 ± 0.284
4.154GluThr: 4.154 ± 0.271
4.582GluVal: 4.582 ± 0.303
0.838GluTrp: 0.838 ± 0.14
2.657GluTyr: 2.657 ± 0.236
0.0GluXaa: 0.0 ± 0.0
Phe
2.71PheAla: 2.71 ± 0.203
0.606PheCys: 0.606 ± 0.118
3.566PheAsp: 3.566 ± 0.248
2.764PheGlu: 2.764 ± 0.206
1.765PhePhe: 1.765 ± 0.186
3.102PheGly: 3.102 ± 0.232
0.499PheHis: 0.499 ± 0.111
2.478PheIle: 2.478 ± 0.241
2.639PheLys: 2.639 ± 0.224
2.746PheLeu: 2.746 ± 0.267
1.034PheMet: 1.034 ± 0.137
2.888PheAsn: 2.888 ± 0.202
1.729PhePro: 1.729 ± 0.23
1.729PheGln: 1.729 ± 0.19
1.515PheArg: 1.515 ± 0.145
3.031PheSer: 3.031 ± 0.204
2.942PheThr: 2.942 ± 0.249
2.621PheVal: 2.621 ± 0.309
0.303PheTrp: 0.303 ± 0.076
1.979PheTyr: 1.979 ± 0.153
0.0PheXaa: 0.0 ± 0.0
Gly
6.401GlyAla: 6.401 ± 0.587
0.624GlyCys: 0.624 ± 0.107
5.563GlyAsp: 5.563 ± 0.52
3.976GlyGlu: 3.976 ± 0.223
2.835GlyPhe: 2.835 ± 0.302
7.88GlyGly: 7.88 ± 0.805
1.355GlyHis: 1.355 ± 0.195
4.422GlyIle: 4.422 ± 0.334
3.78GlyLys: 3.78 ± 0.4
4.6GlyLeu: 4.6 ± 0.268
1.747GlyMet: 1.747 ± 0.297
4.814GlyAsn: 4.814 ± 0.645
2.086GlyPro: 2.086 ± 0.212
2.799GlyGln: 2.799 ± 0.233
2.906GlyArg: 2.906 ± 0.253
6.454GlySer: 6.454 ± 0.539
7.328GlyThr: 7.328 ± 0.811
5.046GlyVal: 5.046 ± 0.294
1.034GlyTrp: 1.034 ± 0.165
3.655GlyTyr: 3.655 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
0.624HisAla: 0.624 ± 0.102
0.285HisCys: 0.285 ± 0.066
0.874HisAsp: 0.874 ± 0.14
0.927HisGlu: 0.927 ± 0.146
0.767HisPhe: 0.767 ± 0.14
1.105HisGly: 1.105 ± 0.198
0.285HisHis: 0.285 ± 0.095
0.945HisIle: 0.945 ± 0.113
0.802HisLys: 0.802 ± 0.178
0.998HisLeu: 0.998 ± 0.132
0.41HisMet: 0.41 ± 0.076
0.802HisAsn: 0.802 ± 0.143
0.891HisPro: 0.891 ± 0.174
0.464HisGln: 0.464 ± 0.089
0.606HisArg: 0.606 ± 0.113
0.802HisSer: 0.802 ± 0.098
1.016HisThr: 1.016 ± 0.156
0.927HisVal: 0.927 ± 0.173
0.214HisTrp: 0.214 ± 0.068
0.963HisTyr: 0.963 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
4.332IleAla: 4.332 ± 0.294
0.571IleCys: 0.571 ± 0.136
4.832IleAsp: 4.832 ± 0.255
4.136IleGlu: 4.136 ± 0.283
2.353IlePhe: 2.353 ± 0.188
4.101IleGly: 4.101 ± 0.29
0.535IleHis: 0.535 ± 0.115
3.316IleIle: 3.316 ± 0.236
3.798IleLys: 3.798 ± 0.261
4.029IleLeu: 4.029 ± 0.268
1.07IleMet: 1.07 ± 0.172
4.119IleAsn: 4.119 ± 0.283
2.514IlePro: 2.514 ± 0.291
2.514IleGln: 2.514 ± 0.205
2.389IleArg: 2.389 ± 0.232
4.136IleSer: 4.136 ± 0.388
5.491IleThr: 5.491 ± 0.661
3.833IleVal: 3.833 ± 0.415
0.588IleTrp: 0.588 ± 0.108
1.694IleTyr: 1.694 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
3.851LysAla: 3.851 ± 0.468
0.553LysCys: 0.553 ± 0.105
3.584LysAsp: 3.584 ± 0.377
3.905LysGlu: 3.905 ± 0.473
2.425LysPhe: 2.425 ± 0.262
3.156LysGly: 3.156 ± 0.279
0.927LysHis: 0.927 ± 0.179
3.744LysIle: 3.744 ± 0.328
4.707LysLys: 4.707 ± 0.753
4.582LysLeu: 4.582 ± 0.38
1.373LysMet: 1.373 ± 0.251
2.924LysAsn: 2.924 ± 0.282
1.783LysPro: 1.783 ± 0.195
2.104LysGln: 2.104 ± 0.298
2.514LysArg: 2.514 ± 0.292
3.815LysSer: 3.815 ± 0.36
3.477LysThr: 3.477 ± 0.259
4.065LysVal: 4.065 ± 0.279
0.838LysTrp: 0.838 ± 0.143
2.977LysTyr: 2.977 ± 0.329
0.0LysXaa: 0.0 ± 0.0
Leu
4.511LeuAla: 4.511 ± 0.296
0.695LeuCys: 0.695 ± 0.161
5.581LeuAsp: 5.581 ± 0.333
4.725LeuGlu: 4.725 ± 0.349
2.657LeuPhe: 2.657 ± 0.236
5.081LeuGly: 5.081 ± 0.387
1.159LeuHis: 1.159 ± 0.208
3.958LeuIle: 3.958 ± 0.27
4.439LeuLys: 4.439 ± 0.459
5.26LeuLeu: 5.26 ± 0.464
1.355LeuMet: 1.355 ± 0.296
4.636LeuAsn: 4.636 ± 0.356
2.799LeuPro: 2.799 ± 0.237
2.995LeuGln: 2.995 ± 0.246
3.352LeuArg: 3.352 ± 0.261
4.457LeuSer: 4.457 ± 0.281
5.687LeuThr: 5.687 ± 0.512
4.475LeuVal: 4.475 ± 0.271
0.713LeuTrp: 0.713 ± 0.144
3.174LeuTyr: 3.174 ± 0.195
0.0LeuXaa: 0.0 ± 0.0
Met
1.373MetAla: 1.373 ± 0.201
0.178MetCys: 0.178 ± 0.066
1.212MetAsp: 1.212 ± 0.175
1.391MetGlu: 1.391 ± 0.255
0.82MetPhe: 0.82 ± 0.155
1.266MetGly: 1.266 ± 0.21
0.499MetHis: 0.499 ± 0.131
1.123MetIle: 1.123 ± 0.171
1.409MetLys: 1.409 ± 0.282
1.694MetLeu: 1.694 ± 0.206
0.517MetMet: 0.517 ± 0.102
1.195MetAsn: 1.195 ± 0.192
0.874MetPro: 0.874 ± 0.15
0.945MetGln: 0.945 ± 0.183
1.034MetArg: 1.034 ± 0.19
1.943MetSer: 1.943 ± 0.268
1.373MetThr: 1.373 ± 0.214
1.302MetVal: 1.302 ± 0.189
0.232MetTrp: 0.232 ± 0.075
0.82MetTyr: 0.82 ± 0.106
0.0MetXaa: 0.0 ± 0.0
Asn
3.976AsnAla: 3.976 ± 0.294
0.428AsnCys: 0.428 ± 0.1
3.584AsnAsp: 3.584 ± 0.238
3.156AsnGlu: 3.156 ± 0.184
2.567AsnPhe: 2.567 ± 0.231
4.796AsnGly: 4.796 ± 0.432
0.678AsnHis: 0.678 ± 0.09
3.744AsnIle: 3.744 ± 0.403
3.191AsnLys: 3.191 ± 0.302
5.081AsnLeu: 5.081 ± 0.433
0.731AsnMet: 0.731 ± 0.135
3.512AsnAsn: 3.512 ± 0.33
2.924AsnPro: 2.924 ± 0.219
2.282AsnGln: 2.282 ± 0.153
2.246AsnArg: 2.246 ± 0.18
3.637AsnSer: 3.637 ± 0.284
4.529AsnThr: 4.529 ± 0.382
4.315AsnVal: 4.315 ± 0.39
0.66AsnTrp: 0.66 ± 0.107
2.371AsnTyr: 2.371 ± 0.191
0.0AsnXaa: 0.0 ± 0.0
Pro
2.728ProAla: 2.728 ± 0.213
0.339ProCys: 0.339 ± 0.096
2.567ProAsp: 2.567 ± 0.255
2.674ProGlu: 2.674 ± 0.251
1.498ProPhe: 1.498 ± 0.209
3.191ProGly: 3.191 ± 0.286
0.642ProHis: 0.642 ± 0.103
2.282ProIle: 2.282 ± 0.245
1.997ProLys: 1.997 ± 0.285
2.211ProLeu: 2.211 ± 0.19
0.535ProMet: 0.535 ± 0.123
2.318ProAsn: 2.318 ± 0.198
1.551ProPro: 1.551 ± 0.165
1.355ProGln: 1.355 ± 0.147
1.462ProArg: 1.462 ± 0.158
3.102ProSer: 3.102 ± 0.191
2.853ProThr: 2.853 ± 0.247
2.532ProVal: 2.532 ± 0.228
0.624ProTrp: 0.624 ± 0.11
1.515ProTyr: 1.515 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
2.478GlnAla: 2.478 ± 0.176
0.321GlnCys: 0.321 ± 0.104
2.122GlnAsp: 2.122 ± 0.18
2.514GlnGlu: 2.514 ± 0.278
1.551GlnPhe: 1.551 ± 0.164
2.496GlnGly: 2.496 ± 0.24
0.802GlnHis: 0.802 ± 0.122
2.478GlnIle: 2.478 ± 0.219
2.193GlnLys: 2.193 ± 0.311
2.942GlnLeu: 2.942 ± 0.235
0.945GlnMet: 0.945 ± 0.17
1.979GlnAsn: 1.979 ± 0.184
1.337GlnPro: 1.337 ± 0.149
1.747GlnGln: 1.747 ± 0.22
1.783GlnArg: 1.783 ± 0.176
2.264GlnSer: 2.264 ± 0.219
2.353GlnThr: 2.353 ± 0.199
2.906GlnVal: 2.906 ± 0.231
0.642GlnTrp: 0.642 ± 0.122
1.694GlnTyr: 1.694 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
2.764ArgAla: 2.764 ± 0.197
0.25ArgCys: 0.25 ± 0.058
2.3ArgAsp: 2.3 ± 0.186
2.567ArgGlu: 2.567 ± 0.332
2.05ArgPhe: 2.05 ± 0.168
3.013ArgGly: 3.013 ± 0.321
0.749ArgHis: 0.749 ± 0.137
2.817ArgIle: 2.817 ± 0.219
2.692ArgLys: 2.692 ± 0.377
3.441ArgLeu: 3.441 ± 0.232
1.07ArgMet: 1.07 ± 0.204
2.157ArgAsn: 2.157 ± 0.212
1.391ArgPro: 1.391 ± 0.152
1.64ArgGln: 1.64 ± 0.166
1.926ArgArg: 1.926 ± 0.288
2.371ArgSer: 2.371 ± 0.246
2.532ArgThr: 2.532 ± 0.256
3.12ArgVal: 3.12 ± 0.217
0.464ArgTrp: 0.464 ± 0.101
2.3ArgTyr: 2.3 ± 0.245
0.0ArgXaa: 0.0 ± 0.0
Ser
5.973SerAla: 5.973 ± 0.377
0.553SerCys: 0.553 ± 0.125
4.029SerAsp: 4.029 ± 0.271
3.762SerGlu: 3.762 ± 0.28
3.209SerPhe: 3.209 ± 0.333
7.114SerGly: 7.114 ± 0.593
0.927SerHis: 0.927 ± 0.138
3.851SerIle: 3.851 ± 0.463
3.477SerLys: 3.477 ± 0.354
4.671SerLeu: 4.671 ± 0.301
1.658SerMet: 1.658 ± 0.312
4.19SerAsn: 4.19 ± 0.355
2.514SerPro: 2.514 ± 0.293
2.139SerGln: 2.139 ± 0.219
2.371SerArg: 2.371 ± 0.206
5.527SerSer: 5.527 ± 0.563
5.117SerThr: 5.117 ± 0.453
4.368SerVal: 4.368 ± 0.368
0.695SerTrp: 0.695 ± 0.116
2.674SerTyr: 2.674 ± 0.245
0.0SerXaa: 0.0 ± 0.0
Thr
6.312ThrAla: 6.312 ± 0.591
0.339ThrCys: 0.339 ± 0.082
4.475ThrAsp: 4.475 ± 0.422
4.243ThrGlu: 4.243 ± 0.335
3.245ThrPhe: 3.245 ± 0.298
7.399ThrGly: 7.399 ± 1.037
0.909ThrHis: 0.909 ± 0.143
5.046ThrIle: 5.046 ± 0.437
3.209ThrLys: 3.209 ± 0.311
6.329ThrLeu: 6.329 ± 0.458
1.23ThrMet: 1.23 ± 0.15
3.976ThrAsn: 3.976 ± 0.454
3.102ThrPro: 3.102 ± 0.259
2.621ThrGln: 2.621 ± 0.184
2.799ThrArg: 2.799 ± 0.241
5.402ThrSer: 5.402 ± 0.669
6.365ThrThr: 6.365 ± 0.622
5.973ThrVal: 5.973 ± 0.671
0.731ThrTrp: 0.731 ± 0.102
2.906ThrTyr: 2.906 ± 0.284
0.0ThrXaa: 0.0 ± 0.0
Val
4.796ValAla: 4.796 ± 0.321
0.535ValCys: 0.535 ± 0.118
5.135ValAsp: 5.135 ± 0.394
4.475ValGlu: 4.475 ± 0.27
2.87ValPhe: 2.87 ± 0.236
5.153ValGly: 5.153 ± 0.66
0.838ValHis: 0.838 ± 0.14
4.065ValIle: 4.065 ± 0.295
3.708ValLys: 3.708 ± 0.301
3.994ValLeu: 3.994 ± 0.272
1.409ValMet: 1.409 ± 0.209
4.101ValAsn: 4.101 ± 0.305
2.906ValPro: 2.906 ± 0.247
2.443ValGln: 2.443 ± 0.187
2.995ValArg: 2.995 ± 0.21
4.885ValSer: 4.885 ± 0.364
6.312ValThr: 6.312 ± 0.613
4.618ValVal: 4.618 ± 0.363
0.66ValTrp: 0.66 ± 0.098
2.585ValTyr: 2.585 ± 0.262
0.0ValXaa: 0.0 ± 0.0
Trp
0.838TrpAla: 0.838 ± 0.139
0.143TrpCys: 0.143 ± 0.055
0.82TrpAsp: 0.82 ± 0.15
0.66TrpGlu: 0.66 ± 0.142
0.464TrpPhe: 0.464 ± 0.113
0.749TrpGly: 0.749 ± 0.11
0.41TrpHis: 0.41 ± 0.101
0.66TrpIle: 0.66 ± 0.097
0.802TrpLys: 0.802 ± 0.17
0.82TrpLeu: 0.82 ± 0.167
0.303TrpMet: 0.303 ± 0.068
0.891TrpAsn: 0.891 ± 0.142
0.143TrpPro: 0.143 ± 0.043
0.464TrpGln: 0.464 ± 0.073
0.499TrpArg: 0.499 ± 0.085
0.784TrpSer: 0.784 ± 0.117
0.927TrpThr: 0.927 ± 0.129
0.784TrpVal: 0.784 ± 0.115
0.16TrpTrp: 0.16 ± 0.049
0.499TrpTyr: 0.499 ± 0.074
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.336TyrAla: 2.336 ± 0.19
0.571TyrCys: 0.571 ± 0.114
3.049TyrAsp: 3.049 ± 0.266
2.621TyrGlu: 2.621 ± 0.305
1.783TyrPhe: 1.783 ± 0.184
2.353TyrGly: 2.353 ± 0.201
0.553TyrHis: 0.553 ± 0.09
2.71TyrIle: 2.71 ± 0.202
2.229TyrLys: 2.229 ± 0.261
3.245TyrLeu: 3.245 ± 0.255
0.998TyrMet: 0.998 ± 0.182
2.46TyrAsn: 2.46 ± 0.211
1.515TyrPro: 1.515 ± 0.165
1.676TyrGln: 1.676 ± 0.161
2.3TyrArg: 2.3 ± 0.2
2.692TyrSer: 2.692 ± 0.236
3.281TyrThr: 3.281 ± 0.419
3.102TyrVal: 3.102 ± 0.223
0.464TyrTrp: 0.464 ± 0.103
1.783TyrTyr: 1.783 ± 0.157
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 210 proteins (56089 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski