Natribacillus halophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Natribacillus

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8RM68|A0A1G8RM68_9BACI Multimeric flavodoxin WrbA OS=Natribacillus halophilus OX=549003 GN=SAMN04488123_1197 PE=4 SV=1
MM1 pKa = 7.5SNGKK5 pKa = 7.56TRR7 pKa = 11.84MLFLSSSLVTAVMFTPMWSEE27 pKa = 3.87NVSAYY32 pKa = 10.44APDD35 pKa = 4.0EE36 pKa = 4.82DD37 pKa = 5.49DD38 pKa = 6.0PNAQDD43 pKa = 3.64YY44 pKa = 9.73TEE46 pKa = 4.83EE47 pKa = 5.4DD48 pKa = 3.42MDD50 pKa = 4.07NSQPYY55 pKa = 9.39LLKK58 pKa = 10.17HH59 pKa = 6.04DD60 pKa = 3.88QGSDD64 pKa = 3.45VEE66 pKa = 4.48QLQSDD71 pKa = 4.04LGDD74 pKa = 3.05RR75 pKa = 11.84GYY77 pKa = 11.25NVQVDD82 pKa = 5.04GIFGPEE88 pKa = 3.6TEE90 pKa = 4.61NAVKK94 pKa = 10.17AYY96 pKa = 10.47QSDD99 pKa = 3.3QGLDD103 pKa = 3.3RR104 pKa = 11.84DD105 pKa = 4.42GVAGSNTYY113 pKa = 9.53QALASDD119 pKa = 4.05EE120 pKa = 4.41TLDD123 pKa = 4.63EE124 pKa = 4.71EE125 pKa = 5.4PDD127 pKa = 3.69TATDD131 pKa = 3.63EE132 pKa = 5.18APDD135 pKa = 3.51TSRR138 pKa = 11.84NGEE141 pKa = 3.95LSNEE145 pKa = 3.87NLEE148 pKa = 4.45NQDD151 pKa = 3.27SSASEE156 pKa = 3.67IAATAEE162 pKa = 4.4SVLGTPYY169 pKa = 10.59VWGGTTPDD177 pKa = 4.03GFDD180 pKa = 2.88SSGFINYY187 pKa = 8.83VFDD190 pKa = 3.67QNGIDD195 pKa = 3.68VSRR198 pKa = 11.84THH200 pKa = 8.63AEE202 pKa = 3.24MWEE205 pKa = 3.93NDD207 pKa = 3.91GEE209 pKa = 4.8HH210 pKa = 5.39VTSMSIGDD218 pKa = 3.48VVFFEE223 pKa = 4.49GTYY226 pKa = 9.0DD227 pKa = 3.37TTGASHH233 pKa = 6.31SGIYY237 pKa = 10.04IGDD240 pKa = 3.51NQMIHH245 pKa = 6.63AGSEE249 pKa = 4.29GVVQADD255 pKa = 3.21ITSDD259 pKa = 2.79YY260 pKa = 9.4WQDD263 pKa = 2.94HH264 pKa = 6.33FIGFKK269 pKa = 10.12TMQQ272 pKa = 3.35

Molecular weight: 29.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8KHB6|A0A1G8KHB6_9BACI Uncharacterized protein OS=Natribacillus halophilus OX=549003 GN=SAMN04488123_102102 PE=4 SV=1
MM1 pKa = 7.23TLEE4 pKa = 4.3SCCYY8 pKa = 8.23TPKK11 pKa = 10.61AGYY14 pKa = 8.7LAPRR18 pKa = 11.84GTRR21 pKa = 11.84SALLYY26 pKa = 8.53PKK28 pKa = 10.37SRR30 pKa = 11.84VSHH33 pKa = 5.16SRR35 pKa = 11.84RR36 pKa = 11.84TRR38 pKa = 11.84SALLYY43 pKa = 8.47PKK45 pKa = 10.45GRR47 pKa = 11.84VSHH50 pKa = 5.85SEE52 pKa = 3.62RR53 pKa = 11.84DD54 pKa = 3.45KK55 pKa = 11.28KK56 pKa = 10.77RR57 pKa = 11.84VVIPQRR63 pKa = 11.84QGISLRR69 pKa = 11.84EE70 pKa = 4.01GPEE73 pKa = 3.88ACCYY77 pKa = 8.47TPKK80 pKa = 10.62AGYY83 pKa = 7.53LTPRR87 pKa = 11.84GTRR90 pKa = 11.84SALLYY95 pKa = 9.27PKK97 pKa = 10.34SRR99 pKa = 11.84VSRR102 pKa = 11.84SEE104 pKa = 3.61RR105 pKa = 11.84DD106 pKa = 3.41KK107 pKa = 11.45KK108 pKa = 10.58RR109 pKa = 11.84VAIPQKK115 pKa = 10.21QGISLRR121 pKa = 11.84EE122 pKa = 3.94GQEE125 pKa = 3.95ACCYY129 pKa = 8.15TPKK132 pKa = 10.55AGYY135 pKa = 7.19PAPRR139 pKa = 11.84GTKK142 pKa = 8.62NHH144 pKa = 6.28VIIPRR149 pKa = 11.84TTGIEE154 pKa = 4.22PIRR157 pKa = 11.84EE158 pKa = 3.86GDD160 pKa = 3.73VILPRR165 pKa = 11.84ARR167 pKa = 11.84LRR169 pKa = 11.84KK170 pKa = 9.86AIFISLKK177 pKa = 10.42NRR179 pKa = 11.84FTLTLPWPISSFCCDD194 pKa = 2.79WHH196 pKa = 7.59SEE198 pKa = 4.17PLQSTT203 pKa = 3.96

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3434

0

3434

952482

27

1818

277.4

31.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.008 ± 0.041

0.641 ± 0.011

6.008 ± 0.042

8.273 ± 0.062

4.17 ± 0.035

7.24 ± 0.04

2.38 ± 0.023

6.812 ± 0.042

4.972 ± 0.045

9.373 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.003 ± 0.022

3.807 ± 0.027

3.86 ± 0.024

3.961 ± 0.031

4.768 ± 0.036

5.597 ± 0.028

5.595 ± 0.026

7.207 ± 0.033

1.047 ± 0.014

3.276 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski