Amino acid dipepetide frequency for Mycobacterium phage Alexphander

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.806AlaAla: 13.806 ± 1.604
0.92AlaCys: 0.92 ± 0.222
6.768AlaAsp: 6.768 ± 0.618
7.201AlaGlu: 7.201 ± 0.801
2.978AlaPhe: 2.978 ± 0.372
9.8AlaGly: 9.8 ± 1.415
2.382AlaHis: 2.382 ± 0.421
4.548AlaIle: 4.548 ± 0.431
4.548AlaLys: 4.548 ± 0.46
8.446AlaLeu: 8.446 ± 0.691
2.761AlaMet: 2.761 ± 0.453
3.194AlaAsn: 3.194 ± 0.559
5.198AlaPro: 5.198 ± 0.612
3.519AlaGln: 3.519 ± 0.479
6.064AlaArg: 6.064 ± 0.578
5.847AlaSer: 5.847 ± 0.644
6.28AlaThr: 6.28 ± 0.581
6.443AlaVal: 6.443 ± 0.671
2.599AlaTrp: 2.599 ± 0.39
2.491AlaTyr: 2.491 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
1.408CysAla: 1.408 ± 0.387
0.162CysCys: 0.162 ± 0.103
1.029CysAsp: 1.029 ± 0.231
0.65CysGlu: 0.65 ± 0.206
0.271CysPhe: 0.271 ± 0.13
1.733CysGly: 1.733 ± 0.322
0.271CysHis: 0.271 ± 0.119
0.217CysIle: 0.217 ± 0.121
0.487CysLys: 0.487 ± 0.149
0.704CysLeu: 0.704 ± 0.23
0.162CysMet: 0.162 ± 0.093
0.325CysAsn: 0.325 ± 0.116
1.029CysPro: 1.029 ± 0.248
0.487CysGln: 0.487 ± 0.192
0.866CysArg: 0.866 ± 0.271
0.65CysSer: 0.65 ± 0.242
0.704CysThr: 0.704 ± 0.173
0.92CysVal: 0.92 ± 0.226
0.271CysTrp: 0.271 ± 0.11
0.379CysTyr: 0.379 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
6.984AspAla: 6.984 ± 0.658
0.92AspCys: 0.92 ± 0.224
4.981AspAsp: 4.981 ± 0.621
3.303AspGlu: 3.303 ± 0.45
1.462AspPhe: 1.462 ± 0.229
6.064AspGly: 6.064 ± 0.468
1.462AspHis: 1.462 ± 0.274
2.491AspIle: 2.491 ± 0.383
1.733AspLys: 1.733 ± 0.33
5.901AspLeu: 5.901 ± 0.582
1.029AspMet: 1.029 ± 0.25
1.733AspAsn: 1.733 ± 0.344
4.819AspPro: 4.819 ± 0.659
2.599AspGln: 2.599 ± 0.33
5.306AspArg: 5.306 ± 0.625
3.086AspSer: 3.086 ± 0.379
3.898AspThr: 3.898 ± 0.465
4.873AspVal: 4.873 ± 0.616
1.516AspTrp: 1.516 ± 0.274
2.328AspTyr: 2.328 ± 0.348
0.0AspXaa: 0.0 ± 0.0
Glu
6.226GluAla: 6.226 ± 0.618
0.92GluCys: 0.92 ± 0.254
3.303GluAsp: 3.303 ± 0.382
3.194GluGlu: 3.194 ± 0.403
2.382GluPhe: 2.382 ± 0.306
3.249GluGly: 3.249 ± 0.416
1.245GluHis: 1.245 ± 0.338
2.707GluIle: 2.707 ± 0.392
1.787GluLys: 1.787 ± 0.34
5.577GluLeu: 5.577 ± 0.713
1.354GluMet: 1.354 ± 0.255
1.949GluAsn: 1.949 ± 0.269
2.978GluPro: 2.978 ± 0.44
3.303GluGln: 3.303 ± 0.459
5.252GluArg: 5.252 ± 0.617
3.465GluSer: 3.465 ± 0.457
3.844GluThr: 3.844 ± 0.548
3.898GluVal: 3.898 ± 0.501
0.812GluTrp: 0.812 ± 0.217
1.57GluTyr: 1.57 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
3.465PheAla: 3.465 ± 0.402
0.487PheCys: 0.487 ± 0.151
2.707PheAsp: 2.707 ± 0.433
1.299PheGlu: 1.299 ± 0.244
0.704PhePhe: 0.704 ± 0.252
3.411PheGly: 3.411 ± 0.714
0.758PheHis: 0.758 ± 0.228
1.191PheIle: 1.191 ± 0.309
1.191PheLys: 1.191 ± 0.307
1.949PheLeu: 1.949 ± 0.28
0.975PheMet: 0.975 ± 0.229
1.191PheAsn: 1.191 ± 0.289
1.624PhePro: 1.624 ± 0.303
0.812PheGln: 0.812 ± 0.322
1.733PheArg: 1.733 ± 0.383
1.624PheSer: 1.624 ± 0.303
2.274PheThr: 2.274 ± 0.383
2.112PheVal: 2.112 ± 0.325
0.866PheTrp: 0.866 ± 0.208
0.812PheTyr: 0.812 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
9.529GlyAla: 9.529 ± 1.286
1.245GlyCys: 1.245 ± 0.321
6.172GlyAsp: 6.172 ± 0.522
4.494GlyGlu: 4.494 ± 0.538
2.707GlyPhe: 2.707 ± 0.482
10.125GlyGly: 10.125 ± 2.082
1.787GlyHis: 1.787 ± 0.29
4.006GlyIle: 4.006 ± 0.497
3.032GlyLys: 3.032 ± 0.346
6.064GlyLeu: 6.064 ± 0.488
2.22GlyMet: 2.22 ± 0.419
3.032GlyAsn: 3.032 ± 0.369
3.952GlyPro: 3.952 ± 0.477
2.707GlyGln: 2.707 ± 0.615
4.981GlyArg: 4.981 ± 0.571
5.847GlySer: 5.847 ± 0.908
6.226GlyThr: 6.226 ± 0.752
6.01GlyVal: 6.01 ± 0.701
2.436GlyTrp: 2.436 ± 0.367
2.815GlyTyr: 2.815 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
1.787HisAla: 1.787 ± 0.311
0.379HisCys: 0.379 ± 0.161
1.462HisAsp: 1.462 ± 0.262
1.354HisGlu: 1.354 ± 0.313
0.379HisPhe: 0.379 ± 0.121
2.057HisGly: 2.057 ± 0.306
1.083HisHis: 1.083 ± 0.331
1.57HisIle: 1.57 ± 0.296
0.866HisLys: 0.866 ± 0.263
1.787HisLeu: 1.787 ± 0.338
0.271HisMet: 0.271 ± 0.1
0.812HisAsn: 0.812 ± 0.222
1.245HisPro: 1.245 ± 0.241
0.704HisGln: 0.704 ± 0.214
2.003HisArg: 2.003 ± 0.398
0.812HisSer: 0.812 ± 0.209
1.408HisThr: 1.408 ± 0.294
1.408HisVal: 1.408 ± 0.346
0.487HisTrp: 0.487 ± 0.175
0.975HisTyr: 0.975 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
5.143IleAla: 5.143 ± 0.546
0.65IleCys: 0.65 ± 0.255
3.628IleAsp: 3.628 ± 0.4
3.303IleGlu: 3.303 ± 0.374
0.704IlePhe: 0.704 ± 0.218
4.277IleGly: 4.277 ± 0.459
1.299IleHis: 1.299 ± 0.285
1.245IleIle: 1.245 ± 0.216
1.029IleLys: 1.029 ± 0.217
1.678IleLeu: 1.678 ± 0.293
0.379IleMet: 0.379 ± 0.141
1.787IleAsn: 1.787 ± 0.304
2.707IlePro: 2.707 ± 0.366
1.678IleGln: 1.678 ± 0.278
2.599IleArg: 2.599 ± 0.374
2.057IleSer: 2.057 ± 0.491
3.682IleThr: 3.682 ± 0.419
3.465IleVal: 3.465 ± 0.377
0.866IleTrp: 0.866 ± 0.21
0.866IleTyr: 0.866 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
4.115LysAla: 4.115 ± 0.505
0.379LysCys: 0.379 ± 0.147
1.949LysAsp: 1.949 ± 0.355
1.191LysGlu: 1.191 ± 0.262
1.245LysPhe: 1.245 ± 0.233
2.382LysGly: 2.382 ± 0.378
1.245LysHis: 1.245 ± 0.311
1.191LysIle: 1.191 ± 0.268
1.462LysLys: 1.462 ± 0.337
2.924LysLeu: 2.924 ± 0.499
0.92LysMet: 0.92 ± 0.222
0.975LysAsn: 0.975 ± 0.265
2.112LysPro: 2.112 ± 0.294
1.516LysGln: 1.516 ± 0.245
2.599LysArg: 2.599 ± 0.335
1.949LysSer: 1.949 ± 0.292
1.841LysThr: 1.841 ± 0.351
2.112LysVal: 2.112 ± 0.324
1.029LysTrp: 1.029 ± 0.296
0.866LysTyr: 0.866 ± 0.251
0.0LysXaa: 0.0 ± 0.0
Leu
8.392LeuAla: 8.392 ± 0.765
0.758LeuCys: 0.758 ± 0.28
4.819LeuAsp: 4.819 ± 0.588
4.277LeuGlu: 4.277 ± 0.492
2.166LeuPhe: 2.166 ± 0.368
6.28LeuGly: 6.28 ± 0.619
0.596LeuHis: 0.596 ± 0.147
3.086LeuIle: 3.086 ± 0.386
2.328LeuLys: 2.328 ± 0.316
4.656LeuLeu: 4.656 ± 0.509
1.733LeuMet: 1.733 ± 0.319
2.599LeuAsn: 2.599 ± 0.381
4.981LeuPro: 4.981 ± 0.578
2.761LeuGln: 2.761 ± 0.477
5.577LeuArg: 5.577 ± 0.571
5.198LeuSer: 5.198 ± 0.558
5.198LeuThr: 5.198 ± 0.565
5.36LeuVal: 5.36 ± 0.587
1.354LeuTrp: 1.354 ± 0.313
1.895LeuTyr: 1.895 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
1.949MetAla: 1.949 ± 0.351
0.217MetCys: 0.217 ± 0.124
1.462MetAsp: 1.462 ± 0.33
0.92MetGlu: 0.92 ± 0.185
0.866MetPhe: 0.866 ± 0.271
1.949MetGly: 1.949 ± 0.365
0.271MetHis: 0.271 ± 0.113
0.92MetIle: 0.92 ± 0.236
0.92MetLys: 0.92 ± 0.282
1.678MetLeu: 1.678 ± 0.264
0.433MetMet: 0.433 ± 0.216
0.866MetAsn: 0.866 ± 0.201
1.408MetPro: 1.408 ± 0.259
0.162MetGln: 0.162 ± 0.086
1.462MetArg: 1.462 ± 0.295
2.978MetSer: 2.978 ± 0.423
1.895MetThr: 1.895 ± 0.323
1.624MetVal: 1.624 ± 0.359
0.271MetTrp: 0.271 ± 0.115
0.325MetTyr: 0.325 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.465AsnAla: 3.465 ± 0.442
0.325AsnCys: 0.325 ± 0.156
2.003AsnAsp: 2.003 ± 0.305
1.787AsnGlu: 1.787 ± 0.354
0.758AsnPhe: 0.758 ± 0.277
4.277AsnGly: 4.277 ± 0.641
0.758AsnHis: 0.758 ± 0.163
1.245AsnIle: 1.245 ± 0.41
1.029AsnLys: 1.029 ± 0.228
2.491AsnLeu: 2.491 ± 0.29
0.812AsnMet: 0.812 ± 0.188
1.841AsnAsn: 1.841 ± 0.342
2.545AsnPro: 2.545 ± 0.326
1.137AsnGln: 1.137 ± 0.318
2.328AsnArg: 2.328 ± 0.436
1.57AsnSer: 1.57 ± 0.348
2.22AsnThr: 2.22 ± 0.315
1.462AsnVal: 1.462 ± 0.201
0.433AsnTrp: 0.433 ± 0.155
0.866AsnTyr: 0.866 ± 0.168
0.0AsnXaa: 0.0 ± 0.0
Pro
5.198ProAla: 5.198 ± 0.599
0.704ProCys: 0.704 ± 0.203
4.169ProAsp: 4.169 ± 0.472
5.143ProGlu: 5.143 ± 0.573
2.274ProPhe: 2.274 ± 0.33
6.389ProGly: 6.389 ± 0.666
1.678ProHis: 1.678 ± 0.275
1.841ProIle: 1.841 ± 0.282
1.678ProLys: 1.678 ± 0.29
4.006ProLeu: 4.006 ± 0.527
1.299ProMet: 1.299 ± 0.265
2.274ProAsn: 2.274 ± 0.371
3.519ProPro: 3.519 ± 0.467
2.22ProGln: 2.22 ± 0.31
3.14ProArg: 3.14 ± 0.533
2.815ProSer: 2.815 ± 0.413
2.815ProThr: 2.815 ± 0.363
4.277ProVal: 4.277 ± 0.499
0.812ProTrp: 0.812 ± 0.205
1.895ProTyr: 1.895 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
4.44GlnAla: 4.44 ± 0.592
0.596GlnCys: 0.596 ± 0.234
1.408GlnAsp: 1.408 ± 0.239
1.733GlnGlu: 1.733 ± 0.35
1.137GlnPhe: 1.137 ± 0.231
2.166GlnGly: 2.166 ± 0.364
0.92GlnHis: 0.92 ± 0.188
2.003GlnIle: 2.003 ± 0.293
1.354GlnLys: 1.354 ± 0.236
3.465GlnLeu: 3.465 ± 0.453
0.812GlnMet: 0.812 ± 0.219
0.812GlnAsn: 0.812 ± 0.242
2.166GlnPro: 2.166 ± 0.408
1.841GlnGln: 1.841 ± 0.447
2.436GlnArg: 2.436 ± 0.385
2.545GlnSer: 2.545 ± 0.372
1.787GlnThr: 1.787 ± 0.339
2.436GlnVal: 2.436 ± 0.331
0.758GlnTrp: 0.758 ± 0.169
1.083GlnTyr: 1.083 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
6.335ArgAla: 6.335 ± 0.658
1.029ArgCys: 1.029 ± 0.318
4.494ArgAsp: 4.494 ± 0.598
4.981ArgGlu: 4.981 ± 0.601
2.924ArgPhe: 2.924 ± 0.384
3.79ArgGly: 3.79 ± 0.352
1.408ArgHis: 1.408 ± 0.357
3.682ArgIle: 3.682 ± 0.455
2.274ArgLys: 2.274 ± 0.36
4.819ArgLeu: 4.819 ± 0.557
2.436ArgMet: 2.436 ± 0.332
2.112ArgAsn: 2.112 ± 0.326
3.411ArgPro: 3.411 ± 0.465
2.112ArgGln: 2.112 ± 0.397
5.198ArgArg: 5.198 ± 0.673
4.006ArgSer: 4.006 ± 0.445
3.357ArgThr: 3.357 ± 0.392
5.306ArgVal: 5.306 ± 0.636
1.462ArgTrp: 1.462 ± 0.289
2.328ArgTyr: 2.328 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
5.035SerAla: 5.035 ± 0.674
0.325SerCys: 0.325 ± 0.172
4.006SerAsp: 4.006 ± 0.531
3.573SerGlu: 3.573 ± 0.461
2.274SerPhe: 2.274 ± 0.455
6.822SerGly: 6.822 ± 0.964
1.245SerHis: 1.245 ± 0.247
2.761SerIle: 2.761 ± 0.428
2.328SerLys: 2.328 ± 0.345
3.736SerLeu: 3.736 ± 0.501
1.191SerMet: 1.191 ± 0.298
2.274SerAsn: 2.274 ± 0.511
3.465SerPro: 3.465 ± 0.457
1.841SerGln: 1.841 ± 0.323
3.249SerArg: 3.249 ± 0.473
4.223SerSer: 4.223 ± 0.7
3.357SerThr: 3.357 ± 0.467
4.44SerVal: 4.44 ± 0.542
1.354SerTrp: 1.354 ± 0.28
1.299SerTyr: 1.299 ± 0.237
0.0SerXaa: 0.0 ± 0.0
Thr
6.064ThrAla: 6.064 ± 0.643
0.92ThrCys: 0.92 ± 0.248
3.736ThrAsp: 3.736 ± 0.523
3.628ThrGlu: 3.628 ± 0.416
1.895ThrPhe: 1.895 ± 0.365
5.577ThrGly: 5.577 ± 0.524
1.624ThrHis: 1.624 ± 0.274
3.357ThrIle: 3.357 ± 0.417
2.22ThrLys: 2.22 ± 0.31
4.331ThrLeu: 4.331 ± 0.516
0.975ThrMet: 0.975 ± 0.232
1.895ThrAsn: 1.895 ± 0.353
4.385ThrPro: 4.385 ± 0.524
1.895ThrGln: 1.895 ± 0.302
3.952ThrArg: 3.952 ± 0.421
3.357ThrSer: 3.357 ± 0.385
4.71ThrThr: 4.71 ± 0.728
5.577ThrVal: 5.577 ± 0.715
1.408ThrTrp: 1.408 ± 0.217
2.22ThrTyr: 2.22 ± 0.295
0.0ThrXaa: 0.0 ± 0.0
Val
7.417ValAla: 7.417 ± 0.681
1.191ValCys: 1.191 ± 0.247
5.36ValAsp: 5.36 ± 0.651
3.844ValGlu: 3.844 ± 0.475
2.22ValPhe: 2.22 ± 0.338
5.414ValGly: 5.414 ± 0.583
1.787ValHis: 1.787 ± 0.307
2.653ValIle: 2.653 ± 0.413
2.22ValLys: 2.22 ± 0.381
5.522ValLeu: 5.522 ± 0.625
1.624ValMet: 1.624 ± 0.257
2.491ValAsn: 2.491 ± 0.374
4.277ValPro: 4.277 ± 0.421
2.707ValGln: 2.707 ± 0.356
4.764ValArg: 4.764 ± 0.61
4.385ValSer: 4.385 ± 0.578
5.143ValThr: 5.143 ± 0.551
6.443ValVal: 6.443 ± 0.692
1.516ValTrp: 1.516 ± 0.287
1.462ValTyr: 1.462 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
2.057TrpAla: 2.057 ± 0.351
0.325TrpCys: 0.325 ± 0.128
1.299TrpAsp: 1.299 ± 0.271
1.029TrpGlu: 1.029 ± 0.231
0.866TrpPhe: 0.866 ± 0.2
1.083TrpGly: 1.083 ± 0.309
0.487TrpHis: 0.487 ± 0.165
1.245TrpIle: 1.245 ± 0.278
0.866TrpLys: 0.866 ± 0.207
1.787TrpLeu: 1.787 ± 0.364
0.65TrpMet: 0.65 ± 0.207
0.433TrpAsn: 0.433 ± 0.219
0.92TrpPro: 0.92 ± 0.233
1.083TrpGln: 1.083 ± 0.281
1.841TrpArg: 1.841 ± 0.353
1.408TrpSer: 1.408 ± 0.279
1.245TrpThr: 1.245 ± 0.226
1.57TrpVal: 1.57 ± 0.409
0.812TrpTrp: 0.812 ± 0.236
0.487TrpTyr: 0.487 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.978TyrAla: 2.978 ± 0.409
0.271TyrCys: 0.271 ± 0.109
1.733TyrAsp: 1.733 ± 0.356
2.112TyrGlu: 2.112 ± 0.343
0.975TyrPhe: 0.975 ± 0.225
2.545TyrGly: 2.545 ± 0.455
0.541TyrHis: 0.541 ± 0.156
1.083TyrIle: 1.083 ± 0.236
0.65TyrLys: 0.65 ± 0.179
2.491TyrLeu: 2.491 ± 0.389
0.487TyrMet: 0.487 ± 0.15
0.758TyrAsn: 0.758 ± 0.188
1.408TyrPro: 1.408 ± 0.302
0.812TyrGln: 0.812 ± 0.198
2.112TyrArg: 2.112 ± 0.369
1.029TyrSer: 1.029 ± 0.244
1.841TyrThr: 1.841 ± 0.33
2.707TyrVal: 2.707 ± 0.357
0.433TyrTrp: 0.433 ± 0.146
0.65TyrTyr: 0.65 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (18471 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski