Nocardioides seonyuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3712 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1BLV0|A0A4V1BLV0_9ACTN Uncharacterized protein OS=Nocardioides seonyuensis OX=2518371 GN=EXE58_00740 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84LGTSAAGVAALALILSACGGADD24 pKa = 3.1AGEE27 pKa = 4.45SGSGEE32 pKa = 4.17GAGGTVAVDD41 pKa = 4.1GSSTVEE47 pKa = 4.04PMSKK51 pKa = 10.12AASEE55 pKa = 4.15LLSEE59 pKa = 4.4EE60 pKa = 4.14NADD63 pKa = 3.62VRR65 pKa = 11.84VTVAASGTGGGFEE78 pKa = 4.26KK79 pKa = 10.75FCVGEE84 pKa = 4.1SDD86 pKa = 3.75ISDD89 pKa = 3.29ASRR92 pKa = 11.84PIKK95 pKa = 10.17EE96 pKa = 4.25DD97 pKa = 3.31EE98 pKa = 4.12EE99 pKa = 5.21VPVCEE104 pKa = 4.42EE105 pKa = 3.75NGVEE109 pKa = 4.11YY110 pKa = 10.84TEE112 pKa = 4.6LQVATDD118 pKa = 3.41ALTVVVHH125 pKa = 6.89PDD127 pKa = 3.32LAVDD131 pKa = 4.46CLTTEE136 pKa = 3.92QLVEE140 pKa = 4.11LWHH143 pKa = 6.14PTSKK147 pKa = 8.69VTNWNEE153 pKa = 3.81LDD155 pKa = 3.67PSFPDD160 pKa = 3.54QEE162 pKa = 4.12IALFGPGTDD171 pKa = 3.19SGTYY175 pKa = 10.12DD176 pKa = 3.4YY177 pKa = 10.61MAADD181 pKa = 4.11VIGDD185 pKa = 3.58EE186 pKa = 4.71SEE188 pKa = 4.36TTRR191 pKa = 11.84SDD193 pKa = 3.83YY194 pKa = 11.09EE195 pKa = 4.1SSEE198 pKa = 4.12DD199 pKa = 4.99DD200 pKa = 3.42NVLVQGVSGTEE211 pKa = 3.72GATGYY216 pKa = 10.79FGFTYY221 pKa = 10.65YY222 pKa = 10.73EE223 pKa = 4.16EE224 pKa = 4.59NADD227 pKa = 3.88SLKK230 pKa = 11.08ALAIDD235 pKa = 4.67DD236 pKa = 4.64GNGCVEE242 pKa = 4.41PSVEE246 pKa = 3.93TAQAGEE252 pKa = 4.27YY253 pKa = 9.36TPLARR258 pKa = 11.84PLFIYY263 pKa = 10.4VSNASYY269 pKa = 11.21AEE271 pKa = 4.12KK272 pKa = 10.0PAVAEE277 pKa = 3.96YY278 pKa = 10.98VDD280 pKa = 5.86FYY282 pKa = 11.16IEE284 pKa = 4.01NLEE287 pKa = 4.38QIATAAKK294 pKa = 9.29FIPLSEE300 pKa = 4.11EE301 pKa = 4.09LYY303 pKa = 10.89EE304 pKa = 4.2EE305 pKa = 4.31TKK307 pKa = 10.76SALEE311 pKa = 4.93GISSS315 pKa = 3.58

Molecular weight: 33.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7IJ59|A0A4P7IJ59_9ACTN Urease accessory protein UreG OS=Nocardioides seonyuensis OX=2518371 GN=ureG PE=3 SV=1
MM1 pKa = 7.46LRR3 pKa = 11.84RR4 pKa = 11.84SRR6 pKa = 11.84ARR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PRR14 pKa = 11.84PLGSRR19 pKa = 11.84PPRR22 pKa = 11.84PRR24 pKa = 11.84PRR26 pKa = 11.84ARR28 pKa = 11.84RR29 pKa = 11.84PLRR32 pKa = 11.84VRR34 pKa = 11.84RR35 pKa = 11.84TRR37 pKa = 11.84LLPRR41 pKa = 11.84PCHH44 pKa = 5.54RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84SPGRR53 pKa = 11.84PSAAPGRR60 pKa = 11.84LLATPGRR67 pKa = 11.84AGPQSGRR74 pKa = 11.84RR75 pKa = 11.84TTSRR79 pKa = 11.84AGSPVRR85 pKa = 4.0

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3712

0

3712

1194092

33

2236

321.7

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.682 ± 0.052

0.739 ± 0.011

6.507 ± 0.032

6.033 ± 0.038

2.785 ± 0.021

9.107 ± 0.04

2.299 ± 0.022

3.393 ± 0.022

1.985 ± 0.028

10.285 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.941 ± 0.014

1.714 ± 0.02

5.573 ± 0.032

2.764 ± 0.018

7.689 ± 0.045

5.565 ± 0.029

6.069 ± 0.037

9.487 ± 0.043

1.574 ± 0.016

1.809 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski