Amino acid dipepetide frequency for Vibrio phage vB_pir03

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.487AlaAla: 6.487 ± 0.491
0.857AlaCys: 0.857 ± 0.112
4.162AlaAsp: 4.162 ± 0.236
5.052AlaGlu: 5.052 ± 0.326
3.906AlaPhe: 3.906 ± 0.736
4.785AlaGly: 4.785 ± 0.434
1.28AlaHis: 1.28 ± 0.134
5.052AlaIle: 5.052 ± 0.285
4.996AlaLys: 4.996 ± 0.225
6.32AlaLeu: 6.32 ± 0.315
2.637AlaMet: 2.637 ± 0.166
3.594AlaAsn: 3.594 ± 0.252
2.604AlaPro: 2.604 ± 0.299
2.159AlaGln: 2.159 ± 0.2
3.472AlaArg: 3.472 ± 0.211
4.573AlaSer: 4.573 ± 0.259
5.019AlaThr: 5.019 ± 0.552
5.319AlaVal: 5.319 ± 0.272
0.99AlaTrp: 0.99 ± 0.11
2.593AlaTyr: 2.593 ± 0.174
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.099
0.134CysCys: 0.134 ± 0.041
0.556CysAsp: 0.556 ± 0.084
0.534CysGlu: 0.534 ± 0.07
0.401CysPhe: 0.401 ± 0.076
0.757CysGly: 0.757 ± 0.099
0.389CysHis: 0.389 ± 0.073
0.467CysIle: 0.467 ± 0.07
0.701CysLys: 0.701 ± 0.095
0.846CysLeu: 0.846 ± 0.106
0.345CysMet: 0.345 ± 0.061
0.501CysAsn: 0.501 ± 0.065
0.523CysPro: 0.523 ± 0.091
0.356CysGln: 0.356 ± 0.066
0.679CysArg: 0.679 ± 0.096
0.69CysSer: 0.69 ± 0.089
0.734CysThr: 0.734 ± 0.112
0.757CysVal: 0.757 ± 0.107
0.167CysTrp: 0.167 ± 0.058
0.356CysTyr: 0.356 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
4.484AspAla: 4.484 ± 0.344
0.723AspCys: 0.723 ± 0.088
3.594AspAsp: 3.594 ± 0.267
4.162AspGlu: 4.162 ± 0.264
3.672AspPhe: 3.672 ± 0.183
4.418AspGly: 4.418 ± 0.257
1.102AspHis: 1.102 ± 0.124
4.829AspIle: 4.829 ± 0.26
3.283AspLys: 3.283 ± 0.239
6.02AspLeu: 6.02 ± 0.256
2.059AspMet: 2.059 ± 0.264
2.715AspAsn: 2.715 ± 0.198
2.726AspPro: 2.726 ± 0.245
1.78AspGln: 1.78 ± 0.153
2.838AspArg: 2.838 ± 0.213
3.539AspSer: 3.539 ± 0.205
3.705AspThr: 3.705 ± 0.301
4.384AspVal: 4.384 ± 0.29
0.968AspTrp: 0.968 ± 0.119
2.615AspTyr: 2.615 ± 0.158
0.0AspXaa: 0.0 ± 0.0
Glu
5.308GluAla: 5.308 ± 0.328
0.49GluCys: 0.49 ± 0.073
4.395GluAsp: 4.395 ± 0.222
5.408GluGlu: 5.408 ± 0.358
3.16GluPhe: 3.16 ± 0.191
3.95GluGly: 3.95 ± 0.21
1.613GluHis: 1.613 ± 0.162
4.751GluIle: 4.751 ± 0.344
3.75GluLys: 3.75 ± 0.321
6.265GluLeu: 6.265 ± 0.425
2.415GluMet: 2.415 ± 0.178
2.971GluAsn: 2.971 ± 0.168
1.558GluPro: 1.558 ± 0.143
2.303GluGln: 2.303 ± 0.215
3.405GluArg: 3.405 ± 0.249
3.45GluSer: 3.45 ± 0.217
4.106GluThr: 4.106 ± 0.253
4.874GluVal: 4.874 ± 0.261
0.69GluTrp: 0.69 ± 0.112
2.704GluTyr: 2.704 ± 0.218
0.0GluXaa: 0.0 ± 0.0
Phe
2.693PheAla: 2.693 ± 0.29
0.668PheCys: 0.668 ± 0.092
3.272PheAsp: 3.272 ± 0.268
3.027PheGlu: 3.027 ± 0.2
2.07PhePhe: 2.07 ± 0.164
2.993PheGly: 2.993 ± 0.177
1.024PheHis: 1.024 ± 0.121
2.381PheIle: 2.381 ± 0.178
2.582PheLys: 2.582 ± 0.186
3.149PheLeu: 3.149 ± 0.251
1.536PheMet: 1.536 ± 0.141
3.672PheAsn: 3.672 ± 1.296
1.569PhePro: 1.569 ± 0.125
1.38PheGln: 1.38 ± 0.151
2.259PheArg: 2.259 ± 0.205
3.316PheSer: 3.316 ± 0.179
2.804PheThr: 2.804 ± 0.189
3.127PheVal: 3.127 ± 0.224
0.556PheTrp: 0.556 ± 0.07
1.958PheTyr: 1.958 ± 0.145
0.0PheXaa: 0.0 ± 0.0
Gly
4.718GlyAla: 4.718 ± 0.688
0.712GlyCys: 0.712 ± 0.113
3.694GlyAsp: 3.694 ± 0.202
4.006GlyGlu: 4.006 ± 0.227
2.882GlyPhe: 2.882 ± 0.178
4.117GlyGly: 4.117 ± 0.334
1.046GlyHis: 1.046 ± 0.11
3.884GlyIle: 3.884 ± 0.271
4.496GlyLys: 4.496 ± 0.277
5.063GlyLeu: 5.063 ± 0.243
1.881GlyMet: 1.881 ± 0.169
3.205GlyAsn: 3.205 ± 0.267
1.858GlyPro: 1.858 ± 0.156
2.059GlyGln: 2.059 ± 0.174
3.138GlyArg: 3.138 ± 0.239
4.062GlySer: 4.062 ± 0.281
4.362GlyThr: 4.362 ± 0.242
3.739GlyVal: 3.739 ± 0.267
1.28GlyTrp: 1.28 ± 0.285
2.737GlyTyr: 2.737 ± 0.168
0.0GlyXaa: 0.0 ± 0.0
His
1.524HisAla: 1.524 ± 0.152
0.356HisCys: 0.356 ± 0.072
1.224HisAsp: 1.224 ± 0.108
1.28HisGlu: 1.28 ± 0.127
0.868HisPhe: 0.868 ± 0.097
1.792HisGly: 1.792 ± 0.164
0.701HisHis: 0.701 ± 0.08
1.447HisIle: 1.447 ± 0.128
0.979HisLys: 0.979 ± 0.122
1.914HisLeu: 1.914 ± 0.181
0.579HisMet: 0.579 ± 0.09
0.99HisAsn: 0.99 ± 0.111
0.946HisPro: 0.946 ± 0.117
0.768HisGln: 0.768 ± 0.1
1.191HisArg: 1.191 ± 0.137
1.057HisSer: 1.057 ± 0.115
1.313HisThr: 1.313 ± 0.112
1.636HisVal: 1.636 ± 0.163
0.356HisTrp: 0.356 ± 0.065
0.879HisTyr: 0.879 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
4.829IleAla: 4.829 ± 0.234
0.679IleCys: 0.679 ± 0.1
4.562IleAsp: 4.562 ± 0.303
3.884IleGlu: 3.884 ± 0.259
1.936IlePhe: 1.936 ± 0.144
3.683IleGly: 3.683 ± 0.199
1.435IleHis: 1.435 ± 0.129
3.361IleIle: 3.361 ± 0.293
3.75IleLys: 3.75 ± 0.277
4.64IleLeu: 4.64 ± 0.332
1.547IleMet: 1.547 ± 0.172
3.26IleAsn: 3.26 ± 0.242
2.815IlePro: 2.815 ± 0.158
2.226IleGln: 2.226 ± 0.19
3.483IleArg: 3.483 ± 0.274
4.373IleSer: 4.373 ± 0.225
4.273IleThr: 4.273 ± 0.195
4.117IleVal: 4.117 ± 0.216
0.534IleTrp: 0.534 ± 0.073
1.825IleTyr: 1.825 ± 0.151
0.0IleXaa: 0.0 ± 0.0
Lys
4.963LysAla: 4.963 ± 0.293
0.434LysCys: 0.434 ± 0.067
3.817LysAsp: 3.817 ± 0.257
5.174LysGlu: 5.174 ± 0.397
2.059LysPhe: 2.059 ± 0.169
2.993LysGly: 2.993 ± 0.228
1.369LysHis: 1.369 ± 0.163
3.483LysIle: 3.483 ± 0.279
3.583LysLys: 3.583 ± 0.341
5.041LysLeu: 5.041 ± 0.289
1.714LysMet: 1.714 ± 0.126
2.348LysAsn: 2.348 ± 0.164
2.515LysPro: 2.515 ± 0.204
2.315LysGln: 2.315 ± 0.212
3.316LysArg: 3.316 ± 0.266
3.238LysSer: 3.238 ± 0.204
3.861LysThr: 3.861 ± 0.225
3.583LysVal: 3.583 ± 0.195
0.734LysTrp: 0.734 ± 0.092
1.981LysTyr: 1.981 ± 0.164
0.0LysXaa: 0.0 ± 0.0
Leu
6.354LeuAla: 6.354 ± 0.253
0.912LeuCys: 0.912 ± 0.115
5.686LeuAsp: 5.686 ± 0.274
6.476LeuGlu: 6.476 ± 0.322
3.594LeuPhe: 3.594 ± 0.281
4.696LeuGly: 4.696 ± 0.283
1.602LeuHis: 1.602 ± 0.149
4.34LeuIle: 4.34 ± 0.313
4.718LeuLys: 4.718 ± 0.324
6.287LeuLeu: 6.287 ± 0.358
2.259LeuMet: 2.259 ± 0.197
4.24LeuAsn: 4.24 ± 0.235
3.472LeuPro: 3.472 ± 0.205
2.548LeuGln: 2.548 ± 0.174
4.596LeuArg: 4.596 ± 0.33
6.053LeuSer: 6.053 ± 0.287
5.82LeuThr: 5.82 ± 0.225
5.931LeuVal: 5.931 ± 0.255
0.846LeuTrp: 0.846 ± 0.084
2.96LeuTyr: 2.96 ± 0.227
0.0LeuXaa: 0.0 ± 0.0
Met
2.537MetAla: 2.537 ± 0.184
0.356MetCys: 0.356 ± 0.065
1.892MetAsp: 1.892 ± 0.136
2.036MetGlu: 2.036 ± 0.173
2.392MetPhe: 2.392 ± 0.87
1.858MetGly: 1.858 ± 0.122
0.657MetHis: 0.657 ± 0.108
1.536MetIle: 1.536 ± 0.155
1.725MetLys: 1.725 ± 0.175
2.259MetLeu: 2.259 ± 0.159
0.757MetMet: 0.757 ± 0.095
1.38MetAsn: 1.38 ± 0.115
1.113MetPro: 1.113 ± 0.121
1.024MetGln: 1.024 ± 0.121
1.714MetArg: 1.714 ± 0.13
2.448MetSer: 2.448 ± 0.217
1.97MetThr: 1.97 ± 0.14
1.691MetVal: 1.691 ± 0.173
0.234MetTrp: 0.234 ± 0.057
0.801MetTyr: 0.801 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
4.306AsnAla: 4.306 ± 0.362
0.445AsnCys: 0.445 ± 0.067
2.481AsnAsp: 2.481 ± 0.162
2.604AsnGlu: 2.604 ± 0.174
2.114AsnPhe: 2.114 ± 0.226
3.772AsnGly: 3.772 ± 0.416
1.257AsnHis: 1.257 ± 0.118
2.871AsnIle: 2.871 ± 0.157
3.127AsnLys: 3.127 ± 0.176
4.284AsnLeu: 4.284 ± 0.217
1.513AsnMet: 1.513 ± 0.24
2.348AsnAsn: 2.348 ± 0.234
2.47AsnPro: 2.47 ± 0.16
2.259AsnGln: 2.259 ± 0.585
2.259AsnArg: 2.259 ± 0.185
2.838AsnSer: 2.838 ± 0.177
3.238AsnThr: 3.238 ± 0.266
3.416AsnVal: 3.416 ± 0.334
1.046AsnTrp: 1.046 ± 0.203
1.714AsnTyr: 1.714 ± 0.128
0.0AsnXaa: 0.0 ± 0.0
Pro
2.782ProAla: 2.782 ± 0.223
0.478ProCys: 0.478 ± 0.072
2.37ProAsp: 2.37 ± 0.176
3.138ProGlu: 3.138 ± 0.232
1.636ProPhe: 1.636 ± 0.165
2.17ProGly: 2.17 ± 0.151
0.857ProHis: 0.857 ± 0.115
2.515ProIle: 2.515 ± 0.169
2.448ProLys: 2.448 ± 0.199
3.327ProLeu: 3.327 ± 0.237
1.013ProMet: 1.013 ± 0.105
1.936ProAsn: 1.936 ± 0.163
1.102ProPro: 1.102 ± 0.117
1.269ProGln: 1.269 ± 0.127
1.836ProArg: 1.836 ± 0.167
2.604ProSer: 2.604 ± 0.186
2.381ProThr: 2.381 ± 0.197
3.394ProVal: 3.394 ± 0.2
0.334ProTrp: 0.334 ± 0.064
1.057ProTyr: 1.057 ± 0.141
0.0ProXaa: 0.0 ± 0.0
Gln
2.671GlnAla: 2.671 ± 0.193
0.289GlnCys: 0.289 ± 0.057
2.259GlnAsp: 2.259 ± 0.335
1.892GlnGlu: 1.892 ± 0.162
1.547GlnPhe: 1.547 ± 0.122
1.424GlnGly: 1.424 ± 0.132
0.835GlnHis: 0.835 ± 0.089
2.014GlnIle: 2.014 ± 0.183
1.869GlnLys: 1.869 ± 0.178
2.838GlnLeu: 2.838 ± 0.228
0.868GlnMet: 0.868 ± 0.098
1.636GlnAsn: 1.636 ± 0.145
1.736GlnPro: 1.736 ± 0.353
1.48GlnGln: 1.48 ± 0.185
1.792GlnArg: 1.792 ± 0.154
1.792GlnSer: 1.792 ± 0.149
2.114GlnThr: 2.114 ± 0.144
1.97GlnVal: 1.97 ± 0.183
0.512GlnTrp: 0.512 ± 0.088
1.402GlnTyr: 1.402 ± 0.129
0.0GlnXaa: 0.0 ± 0.0
Arg
3.594ArgAla: 3.594 ± 0.199
0.612ArgCys: 0.612 ± 0.079
3.194ArgAsp: 3.194 ± 0.162
2.982ArgGlu: 2.982 ± 0.215
2.092ArgPhe: 2.092 ± 0.167
3.16ArgGly: 3.16 ± 0.206
1.068ArgHis: 1.068 ± 0.083
3.494ArgIle: 3.494 ± 0.263
2.793ArgLys: 2.793 ± 0.277
4.974ArgLeu: 4.974 ± 0.331
1.58ArgMet: 1.58 ± 0.139
2.715ArgAsn: 2.715 ± 0.179
1.78ArgPro: 1.78 ± 0.169
1.469ArgGln: 1.469 ± 0.152
2.648ArgArg: 2.648 ± 0.26
2.648ArgSer: 2.648 ± 0.199
2.882ArgThr: 2.882 ± 0.214
3.405ArgVal: 3.405 ± 0.224
0.801ArgTrp: 0.801 ± 0.094
1.914ArgTyr: 1.914 ± 0.176
0.0ArgXaa: 0.0 ± 0.0
Ser
4.696SerAla: 4.696 ± 0.407
0.634SerCys: 0.634 ± 0.093
3.772SerAsp: 3.772 ± 0.235
3.872SerGlu: 3.872 ± 0.225
3.594SerPhe: 3.594 ± 0.319
4.918SerGly: 4.918 ± 0.432
1.335SerHis: 1.335 ± 0.154
3.928SerIle: 3.928 ± 0.22
3.516SerLys: 3.516 ± 0.214
5.553SerLeu: 5.553 ± 0.269
2.07SerMet: 2.07 ± 0.24
2.893SerAsn: 2.893 ± 0.214
2.148SerPro: 2.148 ± 0.17
1.814SerGln: 1.814 ± 0.148
2.604SerArg: 2.604 ± 0.189
4.429SerSer: 4.429 ± 0.21
3.939SerThr: 3.939 ± 0.205
4.507SerVal: 4.507 ± 0.226
0.868SerTrp: 0.868 ± 0.181
1.903SerTyr: 1.903 ± 0.137
0.0SerXaa: 0.0 ± 0.0
Thr
5.274ThrAla: 5.274 ± 0.467
0.545ThrCys: 0.545 ± 0.072
4.139ThrAsp: 4.139 ± 0.308
4.429ThrGlu: 4.429 ± 0.227
2.715ThrPhe: 2.715 ± 0.226
4.963ThrGly: 4.963 ± 0.304
1.346ThrHis: 1.346 ± 0.119
4.006ThrIle: 4.006 ± 0.202
3.628ThrLys: 3.628 ± 0.217
4.74ThrLeu: 4.74 ± 0.23
1.825ThrMet: 1.825 ± 0.166
3.338ThrAsn: 3.338 ± 0.334
2.893ThrPro: 2.893 ± 0.185
2.07ThrGln: 2.07 ± 0.168
2.604ThrArg: 2.604 ± 0.245
4.24ThrSer: 4.24 ± 0.475
4.607ThrThr: 4.607 ± 0.468
4.807ThrVal: 4.807 ± 0.268
1.057ThrTrp: 1.057 ± 0.217
2.125ThrTyr: 2.125 ± 0.172
0.0ThrXaa: 0.0 ± 0.0
Val
4.874ValAla: 4.874 ± 0.192
0.634ValCys: 0.634 ± 0.095
4.707ValAsp: 4.707 ± 0.249
4.407ValGlu: 4.407 ± 0.212
3.038ValPhe: 3.038 ± 0.323
3.705ValGly: 3.705 ± 0.223
1.636ValHis: 1.636 ± 0.161
4.017ValIle: 4.017 ± 0.3
4.139ValLys: 4.139 ± 0.27
5.542ValLeu: 5.542 ± 0.303
1.97ValMet: 1.97 ± 0.152
3.728ValAsn: 3.728 ± 0.184
3.071ValPro: 3.071 ± 0.202
1.881ValGln: 1.881 ± 0.144
3.294ValArg: 3.294 ± 0.217
4.952ValSer: 4.952 ± 0.383
5.419ValThr: 5.419 ± 0.591
4.696ValVal: 4.696 ± 0.277
0.846ValTrp: 0.846 ± 0.107
2.359ValTyr: 2.359 ± 0.214
0.0ValXaa: 0.0 ± 0.0
Trp
0.812TrpAla: 0.812 ± 0.105
0.2TrpCys: 0.2 ± 0.089
1.413TrpAsp: 1.413 ± 0.542
0.901TrpGlu: 0.901 ± 0.105
0.568TrpPhe: 0.568 ± 0.075
0.512TrpGly: 0.512 ± 0.098
0.3TrpHis: 0.3 ± 0.063
0.746TrpIle: 0.746 ± 0.086
0.701TrpLys: 0.701 ± 0.084
1.079TrpLeu: 1.079 ± 0.141
0.445TrpMet: 0.445 ± 0.069
1.068TrpAsn: 1.068 ± 0.198
0.334TrpPro: 0.334 ± 0.071
0.534TrpGln: 0.534 ± 0.096
0.556TrpArg: 0.556 ± 0.077
0.801TrpSer: 0.801 ± 0.083
1.035TrpThr: 1.035 ± 0.185
0.946TrpVal: 0.946 ± 0.098
0.1TrpTrp: 0.1 ± 0.038
0.423TrpTyr: 0.423 ± 0.074
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.292TyrAla: 2.292 ± 0.166
0.389TyrCys: 0.389 ± 0.073
2.426TyrAsp: 2.426 ± 0.161
2.303TyrGlu: 2.303 ± 0.187
1.792TyrPhe: 1.792 ± 0.146
2.27TyrGly: 2.27 ± 0.18
0.946TyrHis: 0.946 ± 0.092
2.092TyrIle: 2.092 ± 0.163
1.747TyrLys: 1.747 ± 0.168
3.249TyrLeu: 3.249 ± 0.174
1.358TyrMet: 1.358 ± 0.125
1.825TyrAsn: 1.825 ± 0.131
1.413TyrPro: 1.413 ± 0.158
1.246TyrGln: 1.246 ± 0.128
2.125TyrArg: 2.125 ± 0.153
1.892TyrSer: 1.892 ± 0.18
1.769TyrThr: 1.769 ± 0.13
2.615TyrVal: 2.615 ± 0.216
0.556TyrTrp: 0.556 ± 0.084
1.346TyrTyr: 1.346 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 334 proteins (89868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski