Sulfolobus spindle-shape virus 1 (SSV1)

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P20220|F112_SSV1 Protein F-112 OS=Sulfolobus spindle-shape virus 1 OX=244589 GN=f112 PE=1 SV=1
MM1 pKa = 7.05MVQVPIFDD9 pKa = 4.37LLKK12 pKa = 10.84LLDD15 pKa = 3.68VLGIDD20 pKa = 3.87SISFRR25 pKa = 11.84VLNEE29 pKa = 3.42YY30 pKa = 10.86DD31 pKa = 3.22EE32 pKa = 5.0GFIRR36 pKa = 11.84VDD38 pKa = 3.48YY39 pKa = 11.23LEE41 pKa = 4.82EE42 pKa = 4.42ALQKK46 pKa = 10.93DD47 pKa = 4.22GWSKK51 pKa = 11.21VYY53 pKa = 10.79NEE55 pKa = 4.72SEE57 pKa = 4.23DD58 pKa = 4.07PNYY61 pKa = 10.93CCGNFDD67 pKa = 4.57VEE69 pKa = 4.6YY70 pKa = 10.67ANNGYY75 pKa = 10.22KK76 pKa = 10.01IILRR80 pKa = 11.84CDD82 pKa = 3.1EE83 pKa = 4.91DD84 pKa = 5.65GYY86 pKa = 11.6VFQITINKK94 pKa = 6.04VTT96 pKa = 3.55

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P20200|A82_SSV1 Uncharacterized protein A-82 OS=Sulfolobus spindle-shape virus 1 OX=244589 PE=4 SV=1
MM1 pKa = 7.16FRR3 pKa = 11.84CPICGFKK10 pKa = 9.8TLRR13 pKa = 11.84LFALKK18 pKa = 9.47QHH20 pKa = 5.69TRR22 pKa = 11.84RR23 pKa = 11.84EE24 pKa = 4.25HH25 pKa = 6.77VLVKK29 pKa = 10.54CPICGFTGKK38 pKa = 9.69HH39 pKa = 5.56LSQHH43 pKa = 6.17FYY45 pKa = 10.51SRR47 pKa = 11.84YY48 pKa = 10.49DD49 pKa = 3.31IDD51 pKa = 4.08HH52 pKa = 7.58LIYY55 pKa = 10.51CYY57 pKa = 10.49LFSSFRR63 pKa = 11.84LPKK66 pKa = 10.1NVRR69 pKa = 11.84LAIKK73 pKa = 10.3RR74 pKa = 11.84KK75 pKa = 10.37LEE77 pKa = 3.92VEE79 pKa = 4.12

Molecular weight: 9.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34

0

34

4952

45

792

145.6

16.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.816 ± 0.347

0.747 ± 0.243

3.534 ± 0.485

6.058 ± 0.916

4.402 ± 0.297

6.139 ± 0.674

1.151 ± 0.243

8.805 ± 0.432

7.25 ± 1.123

10.945 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.186

5.129 ± 0.658

3.473 ± 0.363

3.332 ± 0.348

4.14 ± 0.683

6.624 ± 0.617

6.179 ± 0.657

7.29 ± 0.478

1.272 ± 0.209

5.856 ± 0.504
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski