Amino acid dipepetide frequency for Ralstonia phage RSB1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.541AlaAla: 18.541 ± 2.261
0.919AlaCys: 0.919 ± 0.299
7.125AlaAsp: 7.125 ± 0.81
5.593AlaGlu: 5.593 ± 0.669
3.141AlaPhe: 3.141 ± 0.495
10.726AlaGly: 10.726 ± 1.242
2.528AlaHis: 2.528 ± 0.364
4.137AlaIle: 4.137 ± 0.652
7.125AlaLys: 7.125 ± 1.049
10.573AlaLeu: 10.573 ± 1.14
3.754AlaMet: 3.754 ± 0.587
5.057AlaAsn: 5.057 ± 0.954
2.682AlaPro: 2.682 ± 0.392
5.976AlaGln: 5.976 ± 0.933
7.892AlaArg: 7.892 ± 0.847
6.359AlaSer: 6.359 ± 0.784
6.589AlaThr: 6.589 ± 0.857
7.202AlaVal: 7.202 ± 0.68
1.762AlaTrp: 1.762 ± 0.378
3.678AlaTyr: 3.678 ± 0.536
0.0AlaXaa: 0.0 ± 0.0
Cys
0.613CysAla: 0.613 ± 0.193
0.0CysCys: 0.0 ± 0.0
0.69CysAsp: 0.69 ± 0.254
0.536CysGlu: 0.536 ± 0.191
0.153CysPhe: 0.153 ± 0.111
0.69CysGly: 0.69 ± 0.296
0.383CysHis: 0.383 ± 0.28
0.536CysIle: 0.536 ± 0.24
0.46CysLys: 0.46 ± 0.2
0.69CysLeu: 0.69 ± 0.249
0.23CysMet: 0.23 ± 0.158
0.536CysAsn: 0.536 ± 0.246
0.23CysPro: 0.23 ± 0.126
0.306CysGln: 0.306 ± 0.144
0.46CysArg: 0.46 ± 0.202
0.69CysSer: 0.69 ± 0.217
0.23CysThr: 0.23 ± 0.128
0.766CysVal: 0.766 ± 0.275
0.153CysTrp: 0.153 ± 0.093
0.153CysTyr: 0.153 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
7.662AspAla: 7.662 ± 0.836
0.843AspCys: 0.843 ± 0.295
4.597AspAsp: 4.597 ± 1.138
3.984AspGlu: 3.984 ± 0.639
2.528AspPhe: 2.528 ± 0.413
5.363AspGly: 5.363 ± 0.535
0.613AspHis: 0.613 ± 0.21
2.911AspIle: 2.911 ± 0.369
2.758AspLys: 2.758 ± 0.329
5.363AspLeu: 5.363 ± 0.727
2.145AspMet: 2.145 ± 0.285
2.069AspAsn: 2.069 ± 0.437
3.907AspPro: 3.907 ± 0.501
2.375AspGln: 2.375 ± 0.453
2.988AspArg: 2.988 ± 0.457
2.605AspSer: 2.605 ± 0.457
3.907AspThr: 3.907 ± 0.592
4.214AspVal: 4.214 ± 0.485
0.919AspTrp: 0.919 ± 0.282
2.069AspTyr: 2.069 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
6.972GluAla: 6.972 ± 0.813
0.536GluCys: 0.536 ± 0.222
3.141GluAsp: 3.141 ± 0.492
2.988GluGlu: 2.988 ± 0.562
1.915GluPhe: 1.915 ± 0.407
3.601GluGly: 3.601 ± 0.652
1.532GluHis: 1.532 ± 0.353
1.992GluIle: 1.992 ± 0.457
2.452GluLys: 2.452 ± 0.458
4.98GluLeu: 4.98 ± 0.586
2.069GluMet: 2.069 ± 0.437
1.609GluAsn: 1.609 ± 0.377
1.839GluPro: 1.839 ± 0.332
3.448GluGln: 3.448 ± 0.522
3.754GluArg: 3.754 ± 0.716
2.452GluSer: 2.452 ± 0.432
2.452GluThr: 2.452 ± 0.33
3.984GluVal: 3.984 ± 0.548
1.073GluTrp: 1.073 ± 0.36
1.839GluTyr: 1.839 ± 0.358
0.0GluXaa: 0.0 ± 0.0
Phe
3.524PheAla: 3.524 ± 0.515
0.23PheCys: 0.23 ± 0.14
3.524PheAsp: 3.524 ± 0.53
2.069PheGlu: 2.069 ± 0.389
0.613PhePhe: 0.613 ± 0.208
3.907PheGly: 3.907 ± 0.451
0.46PheHis: 0.46 ± 0.175
1.686PheIle: 1.686 ± 0.463
1.456PheLys: 1.456 ± 0.335
2.988PheLeu: 2.988 ± 0.465
0.536PheMet: 0.536 ± 0.174
1.532PheAsn: 1.532 ± 0.396
0.536PhePro: 0.536 ± 0.207
1.686PheGln: 1.686 ± 0.347
1.226PheArg: 1.226 ± 0.257
1.762PheSer: 1.762 ± 0.267
1.992PheThr: 1.992 ± 0.355
1.839PheVal: 1.839 ± 0.406
0.613PheTrp: 0.613 ± 0.194
0.613PheTyr: 0.613 ± 0.181
0.0PheXaa: 0.0 ± 0.0
Gly
7.815GlyAla: 7.815 ± 0.919
0.69GlyCys: 0.69 ± 0.246
5.67GlyAsp: 5.67 ± 0.513
4.214GlyGlu: 4.214 ± 0.595
2.911GlyPhe: 2.911 ± 0.541
7.738GlyGly: 7.738 ± 0.918
1.302GlyHis: 1.302 ± 0.301
3.448GlyIle: 3.448 ± 0.474
5.363GlyLys: 5.363 ± 0.609
5.44GlyLeu: 5.44 ± 0.726
2.298GlyMet: 2.298 ± 0.459
3.601GlyAsn: 3.601 ± 0.575
2.298GlyPro: 2.298 ± 0.494
4.674GlyGln: 4.674 ± 0.677
6.053GlyArg: 6.053 ± 0.712
4.827GlySer: 4.827 ± 0.731
5.593GlyThr: 5.593 ± 0.768
5.21GlyVal: 5.21 ± 0.64
1.226GlyTrp: 1.226 ± 0.329
2.758GlyTyr: 2.758 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
1.762HisAla: 1.762 ± 0.423
0.383HisCys: 0.383 ± 0.18
1.302HisAsp: 1.302 ± 0.324
0.843HisGlu: 0.843 ± 0.343
0.536HisPhe: 0.536 ± 0.171
1.839HisGly: 1.839 ± 0.339
0.69HisHis: 0.69 ± 0.219
0.843HisIle: 0.843 ± 0.222
1.456HisLys: 1.456 ± 0.409
1.149HisLeu: 1.149 ± 0.3
0.69HisMet: 0.69 ± 0.218
0.536HisAsn: 0.536 ± 0.176
0.919HisPro: 0.919 ± 0.288
0.69HisGln: 0.69 ± 0.247
1.302HisArg: 1.302 ± 0.318
1.302HisSer: 1.302 ± 0.337
1.073HisThr: 1.073 ± 0.209
1.302HisVal: 1.302 ± 0.309
0.23HisTrp: 0.23 ± 0.12
0.613HisTyr: 0.613 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
4.674IleAla: 4.674 ± 0.475
0.153IleCys: 0.153 ± 0.104
2.988IleAsp: 2.988 ± 0.399
3.524IleGlu: 3.524 ± 0.535
1.302IlePhe: 1.302 ± 0.33
4.061IleGly: 4.061 ± 0.366
0.919IleHis: 0.919 ± 0.244
1.992IleIle: 1.992 ± 0.343
2.758IleLys: 2.758 ± 0.62
2.298IleLeu: 2.298 ± 0.301
0.919IleMet: 0.919 ± 0.277
1.456IleAsn: 1.456 ± 0.365
1.839IlePro: 1.839 ± 0.394
1.532IleGln: 1.532 ± 0.394
2.682IleArg: 2.682 ± 0.442
2.452IleSer: 2.452 ± 0.393
2.605IleThr: 2.605 ± 0.327
3.295IleVal: 3.295 ± 0.415
0.306IleTrp: 0.306 ± 0.234
0.69IleTyr: 0.69 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
7.279LysAla: 7.279 ± 0.947
0.613LysCys: 0.613 ± 0.208
2.605LysAsp: 2.605 ± 0.383
2.988LysGlu: 2.988 ± 0.546
1.609LysPhe: 1.609 ± 0.262
3.371LysGly: 3.371 ± 0.668
1.073LysHis: 1.073 ± 0.286
1.762LysIle: 1.762 ± 0.344
1.992LysLys: 1.992 ± 0.293
5.823LysLeu: 5.823 ± 0.719
0.613LysMet: 0.613 ± 0.197
1.915LysAsn: 1.915 ± 0.347
2.298LysPro: 2.298 ± 0.507
2.605LysGln: 2.605 ± 0.571
2.835LysArg: 2.835 ± 0.493
2.835LysSer: 2.835 ± 0.495
3.295LysThr: 3.295 ± 0.448
3.141LysVal: 3.141 ± 0.558
0.766LysTrp: 0.766 ± 0.19
1.915LysTyr: 1.915 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
8.964LeuAla: 8.964 ± 0.649
0.536LeuCys: 0.536 ± 0.19
5.287LeuAsp: 5.287 ± 0.611
3.907LeuGlu: 3.907 ± 0.597
2.069LeuPhe: 2.069 ± 0.545
6.359LeuGly: 6.359 ± 0.717
2.222LeuHis: 2.222 ± 0.42
3.831LeuIle: 3.831 ± 0.609
3.601LeuLys: 3.601 ± 0.537
5.976LeuLeu: 5.976 ± 0.549
1.762LeuMet: 1.762 ± 0.338
3.141LeuAsn: 3.141 ± 0.407
3.524LeuPro: 3.524 ± 0.525
4.367LeuGln: 4.367 ± 0.671
5.44LeuArg: 5.44 ± 0.67
5.899LeuSer: 5.899 ± 0.688
5.823LeuThr: 5.823 ± 0.57
4.98LeuVal: 4.98 ± 0.642
0.996LeuTrp: 0.996 ± 0.236
1.226LeuTyr: 1.226 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
3.524MetAla: 3.524 ± 0.51
0.153MetCys: 0.153 ± 0.098
1.686MetAsp: 1.686 ± 0.393
1.149MetGlu: 1.149 ± 0.344
0.536MetPhe: 0.536 ± 0.147
1.762MetGly: 1.762 ± 0.355
0.46MetHis: 0.46 ± 0.195
1.226MetIle: 1.226 ± 0.317
0.996MetLys: 0.996 ± 0.268
2.682MetLeu: 2.682 ± 0.422
0.383MetMet: 0.383 ± 0.179
0.766MetAsn: 0.766 ± 0.244
1.226MetPro: 1.226 ± 0.351
0.766MetGln: 0.766 ± 0.187
1.839MetArg: 1.839 ± 0.42
2.222MetSer: 2.222 ± 0.348
1.456MetThr: 1.456 ± 0.377
1.302MetVal: 1.302 ± 0.308
0.766MetTrp: 0.766 ± 0.247
0.766MetTyr: 0.766 ± 0.218
0.0MetXaa: 0.0 ± 0.0
Asn
5.21AsnAla: 5.21 ± 0.747
0.153AsnCys: 0.153 ± 0.134
1.915AsnAsp: 1.915 ± 0.347
1.839AsnGlu: 1.839 ± 0.328
1.609AsnPhe: 1.609 ± 0.342
3.065AsnGly: 3.065 ± 0.566
0.383AsnHis: 0.383 ± 0.176
1.915AsnIle: 1.915 ± 0.391
1.762AsnLys: 1.762 ± 0.32
3.065AsnLeu: 3.065 ± 0.403
0.843AsnMet: 0.843 ± 0.195
1.532AsnAsn: 1.532 ± 0.364
2.452AsnPro: 2.452 ± 0.337
1.686AsnGln: 1.686 ± 0.361
2.145AsnArg: 2.145 ± 0.355
2.452AsnSer: 2.452 ± 0.391
2.605AsnThr: 2.605 ± 0.438
2.988AsnVal: 2.988 ± 0.535
0.766AsnTrp: 0.766 ± 0.184
0.919AsnTyr: 0.919 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
5.976ProAla: 5.976 ± 0.922
0.153ProCys: 0.153 ± 0.11
3.371ProAsp: 3.371 ± 0.486
2.375ProGlu: 2.375 ± 0.373
1.686ProPhe: 1.686 ± 0.292
4.214ProGly: 4.214 ± 0.549
0.69ProHis: 0.69 ± 0.228
1.456ProIle: 1.456 ± 0.318
2.145ProLys: 2.145 ± 0.362
2.375ProLeu: 2.375 ± 0.433
0.613ProMet: 0.613 ± 0.159
1.456ProAsn: 1.456 ± 0.289
1.226ProPro: 1.226 ± 0.312
1.915ProGln: 1.915 ± 0.43
0.919ProArg: 0.919 ± 0.244
3.295ProSer: 3.295 ± 0.558
2.298ProThr: 2.298 ± 0.395
2.605ProVal: 2.605 ± 0.456
0.766ProTrp: 0.766 ± 0.219
1.762ProTyr: 1.762 ± 0.476
0.0ProXaa: 0.0 ± 0.0
Gln
7.049GlnAla: 7.049 ± 1.15
0.46GlnCys: 0.46 ± 0.211
2.069GlnAsp: 2.069 ± 0.383
2.069GlnGlu: 2.069 ± 0.57
1.456GlnPhe: 1.456 ± 0.487
3.754GlnGly: 3.754 ± 0.832
0.919GlnHis: 0.919 ± 0.266
2.375GlnIle: 2.375 ± 0.433
2.682GlnLys: 2.682 ± 0.333
3.448GlnLeu: 3.448 ± 0.537
0.613GlnMet: 0.613 ± 0.306
1.609GlnAsn: 1.609 ± 0.294
2.452GlnPro: 2.452 ± 0.506
3.218GlnGln: 3.218 ± 0.733
3.678GlnArg: 3.678 ± 0.672
3.065GlnSer: 3.065 ± 0.6
1.992GlnThr: 1.992 ± 0.4
2.222GlnVal: 2.222 ± 0.41
0.536GlnTrp: 0.536 ± 0.182
2.145GlnTyr: 2.145 ± 0.328
0.0GlnXaa: 0.0 ± 0.0
Arg
6.129ArgAla: 6.129 ± 0.786
0.69ArgCys: 0.69 ± 0.285
3.218ArgAsp: 3.218 ± 0.444
4.061ArgGlu: 4.061 ± 0.645
2.911ArgPhe: 2.911 ± 0.41
3.754ArgGly: 3.754 ± 0.563
1.149ArgHis: 1.149 ± 0.36
3.678ArgIle: 3.678 ± 0.683
3.678ArgLys: 3.678 ± 0.617
4.597ArgLeu: 4.597 ± 0.71
1.762ArgMet: 1.762 ± 0.382
2.605ArgAsn: 2.605 ± 0.415
1.915ArgPro: 1.915 ± 0.355
1.915ArgGln: 1.915 ± 0.332
3.984ArgArg: 3.984 ± 0.697
2.605ArgSer: 2.605 ± 0.292
3.601ArgThr: 3.601 ± 0.506
4.597ArgVal: 4.597 ± 0.855
1.379ArgTrp: 1.379 ± 0.294
2.145ArgTyr: 2.145 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
5.823SerAla: 5.823 ± 0.631
0.613SerCys: 0.613 ± 0.253
3.295SerAsp: 3.295 ± 0.442
2.298SerGlu: 2.298 ± 0.391
1.456SerPhe: 1.456 ± 0.313
4.52SerGly: 4.52 ± 0.684
1.149SerHis: 1.149 ± 0.308
3.065SerIle: 3.065 ± 0.375
2.758SerLys: 2.758 ± 0.522
4.827SerLeu: 4.827 ± 0.599
1.609SerMet: 1.609 ± 0.295
2.988SerAsn: 2.988 ± 0.455
3.065SerPro: 3.065 ± 0.514
3.065SerGln: 3.065 ± 0.683
2.605SerArg: 2.605 ± 0.523
3.141SerSer: 3.141 ± 0.755
4.444SerThr: 4.444 ± 0.669
3.295SerVal: 3.295 ± 0.465
1.456SerTrp: 1.456 ± 0.265
1.379SerTyr: 1.379 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
7.508ThrAla: 7.508 ± 0.856
0.383ThrCys: 0.383 ± 0.199
4.291ThrAsp: 4.291 ± 0.576
3.295ThrGlu: 3.295 ± 0.588
2.298ThrPhe: 2.298 ± 0.5
5.21ThrGly: 5.21 ± 0.669
0.766ThrHis: 0.766 ± 0.259
1.762ThrIle: 1.762 ± 0.29
2.682ThrLys: 2.682 ± 0.439
4.52ThrLeu: 4.52 ± 0.663
1.762ThrMet: 1.762 ± 0.333
1.762ThrAsn: 1.762 ± 0.328
3.984ThrPro: 3.984 ± 0.518
2.528ThrGln: 2.528 ± 0.488
2.988ThrArg: 2.988 ± 0.571
2.835ThrSer: 2.835 ± 0.529
3.141ThrThr: 3.141 ± 0.5
4.444ThrVal: 4.444 ± 0.609
0.919ThrTrp: 0.919 ± 0.225
2.145ThrTyr: 2.145 ± 0.467
0.0ThrXaa: 0.0 ± 0.0
Val
7.355ValAla: 7.355 ± 0.844
0.613ValCys: 0.613 ± 0.208
3.601ValAsp: 3.601 ± 0.372
3.831ValGlu: 3.831 ± 0.466
1.762ValPhe: 1.762 ± 0.435
5.21ValGly: 5.21 ± 0.939
1.226ValHis: 1.226 ± 0.337
2.375ValIle: 2.375 ± 0.502
3.371ValLys: 3.371 ± 0.418
5.057ValLeu: 5.057 ± 0.791
1.762ValMet: 1.762 ± 0.291
3.218ValAsn: 3.218 ± 0.546
3.141ValPro: 3.141 ± 0.445
3.218ValGln: 3.218 ± 0.411
4.674ValArg: 4.674 ± 0.515
3.984ValSer: 3.984 ± 0.621
3.524ValThr: 3.524 ± 0.51
5.21ValVal: 5.21 ± 0.567
0.843ValTrp: 0.843 ± 0.212
2.069ValTyr: 2.069 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
1.609TrpAla: 1.609 ± 0.374
0.153TrpCys: 0.153 ± 0.108
1.073TrpAsp: 1.073 ± 0.298
0.996TrpGlu: 0.996 ± 0.245
0.919TrpPhe: 0.919 ± 0.254
0.919TrpGly: 0.919 ± 0.253
0.69TrpHis: 0.69 ± 0.273
0.153TrpIle: 0.153 ± 0.104
0.919TrpLys: 0.919 ± 0.25
1.915TrpLeu: 1.915 ± 0.435
0.919TrpMet: 0.919 ± 0.27
0.536TrpAsn: 0.536 ± 0.172
0.46TrpPro: 0.46 ± 0.221
0.306TrpGln: 0.306 ± 0.136
1.226TrpArg: 1.226 ± 0.371
0.69TrpSer: 0.69 ± 0.24
0.996TrpThr: 0.996 ± 0.278
1.302TrpVal: 1.302 ± 0.37
0.23TrpTrp: 0.23 ± 0.137
0.23TrpTyr: 0.23 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.371TyrAla: 3.371 ± 0.578
0.23TyrCys: 0.23 ± 0.145
2.452TyrAsp: 2.452 ± 0.339
2.069TyrGlu: 2.069 ± 0.346
1.379TyrPhe: 1.379 ± 0.248
3.065TyrGly: 3.065 ± 0.529
0.306TyrHis: 0.306 ± 0.151
1.302TyrIle: 1.302 ± 0.4
0.919TyrLys: 0.919 ± 0.329
2.145TyrLeu: 2.145 ± 0.388
0.306TyrMet: 0.306 ± 0.138
1.379TyrAsn: 1.379 ± 0.361
1.302TyrPro: 1.302 ± 0.348
1.532TyrGln: 1.532 ± 0.362
1.686TyrArg: 1.686 ± 0.366
1.379TyrSer: 1.379 ± 0.317
1.609TyrThr: 1.609 ± 0.435
2.145TyrVal: 2.145 ± 0.438
0.536TyrTrp: 0.536 ± 0.342
0.69TyrTyr: 0.69 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (13053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski