Amino acid dipepetide frequency for Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.711AlaAla: 22.711 ± 0.171	1.042AlaCys: 1.042 ± 0.02	8.236AlaAsp: 8.236 ± 0.075	9.187AlaGlu: 9.187 ± 0.094	3.564AlaPhe: 3.564 ± 0.037	13.79AlaGly: 13.79 ± 0.096	2.924AlaHis: 2.924 ± 0.036	3.285AlaIle: 3.285 ± 0.04	2.912AlaLys: 2.912 ± 0.047	14.956AlaLeu: 14.956 ± 0.11	2.428AlaMet: 2.428 ± 0.033	1.839AlaAsn: 1.839 ± 0.038	8.02AlaPro: 8.02 ± 0.083	3.494AlaGln: 3.494 ± 0.041	10.622AlaArg: 10.622 ± 0.079	5.833AlaSer: 5.833 ± 0.059	7.311AlaThr: 7.311 ± 0.074	12.597AlaVal: 12.597 ± 0.087	1.921AlaTrp: 1.921 ± 0.032	2.856AlaTyr: 2.856 ± 0.034	0.001AlaXaa: 0.001 ± 0.001
Cys	1.069CysAla: 1.069 ± 0.025	0.087CysCys: 0.087 ± 0.006	0.43CysAsp: 0.43 ± 0.013	0.394CysGlu: 0.394 ± 0.014	0.209CysPhe: 0.209 ± 0.01	0.939CysGly: 0.939 ± 0.02	0.19CysHis: 0.19 ± 0.01	0.14CysIle: 0.14 ± 0.007	0.106CysLys: 0.106 ± 0.007	0.741CysLeu: 0.741 ± 0.018	0.122CysMet: 0.122 ± 0.007	0.125CysAsn: 0.125 ± 0.007	0.468CysPro: 0.468 ± 0.015	0.152CysGln: 0.152 ± 0.008	0.595CysArg: 0.595 ± 0.016	0.408CysSer: 0.408 ± 0.013	0.482CysThr: 0.482 ± 0.015	0.644CysVal: 0.644 ± 0.018	0.127CysTrp: 0.127 ± 0.007	0.143CysTyr: 0.143 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.584AspAla: 7.584 ± 0.068	0.417AspCys: 0.417 ± 0.013	3.261AspAsp: 3.261 ± 0.043	3.898AspGlu: 3.898 ± 0.044	1.597AspPhe: 1.597 ± 0.023	6.479AspGly: 6.479 ± 0.061	1.4AspHis: 1.4 ± 0.025	1.724AspIle: 1.724 ± 0.029	1.128AspLys: 1.128 ± 0.026	6.022AspLeu: 6.022 ± 0.048	0.749AspMet: 0.749 ± 0.018	0.864AspAsn: 0.864 ± 0.02	4.726AspPro: 4.726 ± 0.05	1.412AspGln: 1.412 ± 0.022	5.068AspArg: 5.068 ± 0.057	2.349AspSer: 2.349 ± 0.034	3.166AspThr: 3.166 ± 0.041	4.526AspVal: 4.526 ± 0.046	1.026AspTrp: 1.026 ± 0.024	0.989AspTyr: 0.989 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.739GluAla: 7.739 ± 0.086	0.354GluCys: 0.354 ± 0.012	2.877GluAsp: 2.877 ± 0.041	3.73GluGlu: 3.73 ± 0.051	1.44GluPhe: 1.44 ± 0.025	4.511GluGly: 4.511 ± 0.04	1.462GluHis: 1.462 ± 0.026	2.216GluIle: 2.216 ± 0.036	1.483GluLys: 1.483 ± 0.03	6.808GluLeu: 6.808 ± 0.068	0.835GluMet: 0.835 ± 0.019	1.043GluAsn: 1.043 ± 0.021	3.542GluPro: 3.542 ± 0.044	2.054GluGln: 2.054 ± 0.032	5.62GluArg: 5.62 ± 0.054	2.471GluSer: 2.471 ± 0.037	3.088GluThr: 3.088 ± 0.034	4.32GluVal: 4.32 ± 0.051	0.792GluTrp: 0.792 ± 0.021	1.105GluTyr: 1.105 ± 0.019	0.002GluXaa: 0.002 ± 0.001
Phe	3.656PheAla: 3.656 ± 0.039	0.254PheCys: 0.254 ± 0.011	1.867PheAsp: 1.867 ± 0.028	1.393PheGlu: 1.393 ± 0.024	0.869PhePhe: 0.869 ± 0.024	3.055PheGly: 3.055 ± 0.033	0.631PheHis: 0.631 ± 0.016	0.622PheIle: 0.622 ± 0.017	0.513PheLys: 0.513 ± 0.015	2.648PheLeu: 2.648 ± 0.041	0.4PheMet: 0.4 ± 0.015	0.549PheAsn: 0.549 ± 0.017	1.368PhePro: 1.368 ± 0.025	0.643PheGln: 0.643 ± 0.013	1.846PheArg: 1.846 ± 0.028	1.391PheSer: 1.391 ± 0.026	2.023PheThr: 2.023 ± 0.028	2.2PheVal: 2.2 ± 0.032	0.413PheTrp: 0.413 ± 0.014	0.541PheTyr: 0.541 ± 0.014	0.001PheXaa: 0.001 ± 0.0
Gly	11.983GlyAla: 11.983 ± 0.089	0.832GlyCys: 0.832 ± 0.02	5.165GlyAsp: 5.165 ± 0.048	5.213GlyGlu: 5.213 ± 0.048	2.866GlyPhe: 2.866 ± 0.038	9.435GlyGly: 9.435 ± 0.093	2.31GlyHis: 2.31 ± 0.034	3.377GlyIle: 3.377 ± 0.035	2.424GlyLys: 2.424 ± 0.04	9.654GlyLeu: 9.654 ± 0.084	1.9GlyMet: 1.9 ± 0.028	1.735GlyAsn: 1.735 ± 0.036	5.8GlyPro: 5.8 ± 0.067	2.576GlyGln: 2.576 ± 0.04	8.059GlyArg: 8.059 ± 0.065	5.35GlySer: 5.35 ± 0.053	6.723GlyThr: 6.723 ± 0.069	7.581GlyVal: 7.581 ± 0.061	1.798GlyTrp: 1.798 ± 0.028	2.246GlyTyr: 2.246 ± 0.035	0.002GlyXaa: 0.002 ± 0.001
His	2.714HisAla: 2.714 ± 0.036	0.212HisCys: 0.212 ± 0.009	1.295HisAsp: 1.295 ± 0.026	1.195HisGlu: 1.195 ± 0.026	0.611HisPhe: 0.611 ± 0.016	2.413HisGly: 2.413 ± 0.03	0.683HisHis: 0.683 ± 0.017	0.606HisIle: 0.606 ± 0.017	0.335HisLys: 0.335 ± 0.012	2.358HisLeu: 2.358 ± 0.036	0.317HisMet: 0.317 ± 0.012	0.349HisAsn: 0.349 ± 0.012	1.847HisPro: 1.847 ± 0.028	0.665HisGln: 0.665 ± 0.018	2.085HisArg: 2.085 ± 0.034	0.926HisSer: 0.926 ± 0.023	1.297HisThr: 1.297 ± 0.026	1.645HisVal: 1.645 ± 0.028	0.366HisTrp: 0.366 ± 0.014	0.462HisTyr: 0.462 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.562IleAla: 4.562 ± 0.048	0.283IleCys: 0.283 ± 0.01	2.051IleAsp: 2.051 ± 0.03	1.878IleGlu: 1.878 ± 0.031	0.655IlePhe: 0.655 ± 0.018	3.474IleGly: 3.474 ± 0.041	0.577IleHis: 0.577 ± 0.017	0.765IleIle: 0.765 ± 0.02	0.68IleLys: 0.68 ± 0.017	2.302IleLeu: 2.302 ± 0.035	0.417IleMet: 0.417 ± 0.015	0.66IleAsn: 0.66 ± 0.017	1.656IlePro: 1.656 ± 0.028	0.673IleGln: 0.673 ± 0.016	2.137IleArg: 2.137 ± 0.029	1.53IleSer: 1.53 ± 0.026	2.082IleThr: 2.082 ± 0.033	2.631IleVal: 2.631 ± 0.039	0.365IleTrp: 0.365 ± 0.013	0.487IleTyr: 0.487 ± 0.013	0.0IleXaa: 0.0 ± 0.0
Lys	3.091LysAla: 3.091 ± 0.055	0.108LysCys: 0.108 ± 0.007	1.333LysAsp: 1.333 ± 0.028	1.195LysGlu: 1.195 ± 0.027	0.459LysPhe: 0.459 ± 0.014	1.863LysGly: 1.863 ± 0.035	0.424LysHis: 0.424 ± 0.012	0.876LysIle: 0.876 ± 0.022	0.834LysLys: 0.834 ± 0.023	1.984LysLeu: 1.984 ± 0.033	0.355LysMet: 0.355 ± 0.012	0.542LysAsn: 0.542 ± 0.016	1.389LysPro: 1.389 ± 0.031	0.657LysGln: 0.657 ± 0.019	1.374LysArg: 1.374 ± 0.025	1.048LysSer: 1.048 ± 0.024	1.314LysThr: 1.314 ± 0.028	1.853LysVal: 1.853 ± 0.033	0.247LysTrp: 0.247 ± 0.01	0.427LysTyr: 0.427 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.582LeuAla: 15.582 ± 0.121	0.813LeuCys: 0.813 ± 0.02	6.82LeuAsp: 6.82 ± 0.064	4.364LeuGlu: 4.364 ± 0.045	2.597LeuPhe: 2.597 ± 0.037	9.561LeuGly: 9.561 ± 0.077	2.226LeuHis: 2.226 ± 0.034	3.018LeuIle: 3.018 ± 0.047	2.021LeuLys: 2.021 ± 0.033	11.562LeuLeu: 11.562 ± 0.113	1.6LeuMet: 1.6 ± 0.025	1.583LeuAsn: 1.583 ± 0.028	6.566LeuPro: 6.566 ± 0.059	2.061LeuGln: 2.061 ± 0.028	8.668LeuArg: 8.668 ± 0.077	5.121LeuSer: 5.121 ± 0.047	6.996LeuThr: 6.996 ± 0.052	9.02LeuVal: 9.02 ± 0.072	1.35LeuTrp: 1.35 ± 0.027	1.835LeuTyr: 1.835 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	2.17MetAla: 2.17 ± 0.036	0.138MetCys: 0.138 ± 0.007	0.849MetAsp: 0.849 ± 0.018	0.733MetGlu: 0.733 ± 0.015	0.421MetPhe: 0.421 ± 0.015	1.304MetGly: 1.304 ± 0.024	0.326MetHis: 0.326 ± 0.011	0.639MetIle: 0.639 ± 0.019	0.425MetLys: 0.425 ± 0.016	1.568MetLeu: 1.568 ± 0.027	0.285MetMet: 0.285 ± 0.011	0.461MetAsn: 0.461 ± 0.015	1.065MetPro: 1.065 ± 0.021	0.403MetGln: 0.403 ± 0.014	1.365MetArg: 1.365 ± 0.024	1.264MetSer: 1.264 ± 0.021	1.609MetThr: 1.609 ± 0.025	1.183MetVal: 1.183 ± 0.024	0.189MetTrp: 0.189 ± 0.009	0.319MetTyr: 0.319 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.146AsnAla: 2.146 ± 0.04	0.148AsnCys: 0.148 ± 0.009	0.931AsnAsp: 0.931 ± 0.023	0.792AsnGlu: 0.792 ± 0.019	0.46AsnPhe: 0.46 ± 0.015	1.878AsnGly: 1.878 ± 0.035	0.381AsnHis: 0.381 ± 0.014	0.606AsnIle: 0.606 ± 0.017	0.396AsnLys: 0.396 ± 0.015	1.564AsnLeu: 1.564 ± 0.026	0.291AsnMet: 0.291 ± 0.01	0.393AsnAsn: 0.393 ± 0.014	1.346AsnPro: 1.346 ± 0.03	0.504AsnGln: 0.504 ± 0.017	1.244AsnArg: 1.244 ± 0.025	0.877AsnSer: 0.877 ± 0.022	1.069AsnThr: 1.069 ± 0.027	1.288AsnVal: 1.288 ± 0.025	0.285AsnTrp: 0.285 ± 0.012	0.373AsnTyr: 0.373 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.551ProAla: 9.551 ± 0.087	0.322ProCys: 0.322 ± 0.013	4.389ProAsp: 4.389 ± 0.053	4.722ProGlu: 4.722 ± 0.059	1.549ProPhe: 1.549 ± 0.026	7.163ProGly: 7.163 ± 0.073	1.367ProHis: 1.367 ± 0.026	1.175ProIle: 1.175 ± 0.023	1.19ProLys: 1.19 ± 0.03	5.591ProLeu: 5.591 ± 0.054	1.027ProMet: 1.027 ± 0.022	0.866ProAsn: 0.866 ± 0.024	3.961ProPro: 3.961 ± 0.074	1.679ProGln: 1.679 ± 0.032	4.217ProArg: 4.217 ± 0.05	3.164ProSer: 3.164 ± 0.045	3.467ProThr: 3.467 ± 0.054	5.838ProVal: 5.838 ± 0.057	0.948ProTrp: 0.948 ± 0.023	1.516ProTyr: 1.516 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	3.449GlnAla: 3.449 ± 0.045	0.154GlnCys: 0.154 ± 0.008	1.302GlnAsp: 1.302 ± 0.021	1.443GlnGlu: 1.443 ± 0.025	0.629GlnPhe: 0.629 ± 0.017	2.226GlnGly: 2.226 ± 0.032	0.618GlnHis: 0.618 ± 0.013	1.061GlnIle: 1.061 ± 0.02	0.601GlnLys: 0.601 ± 0.018	2.835GlnLeu: 2.835 ± 0.038	0.433GlnMet: 0.433 ± 0.012	0.506GlnAsn: 0.506 ± 0.014	1.59GlnPro: 1.59 ± 0.029	1.154GlnGln: 1.154 ± 0.033	2.197GlnArg: 2.197 ± 0.032	1.135GlnSer: 1.135 ± 0.021	1.283GlnThr: 1.283 ± 0.027	2.184GlnVal: 2.184 ± 0.028	0.431GlnTrp: 0.431 ± 0.014	0.595GlnTyr: 0.595 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	10.285ArgAla: 10.285 ± 0.093	0.593ArgCys: 0.593 ± 0.016	4.149ArgAsp: 4.149 ± 0.049	4.74ArgGlu: 4.74 ± 0.047	2.331ArgPhe: 2.331 ± 0.033	6.008ArgGly: 6.008 ± 0.056	2.074ArgHis: 2.074 ± 0.032	3.198ArgIle: 3.198 ± 0.038	1.64ArgLys: 1.64 ± 0.025	8.784ArgLeu: 8.784 ± 0.074	1.696ArgMet: 1.696 ± 0.024	1.332ArgAsn: 1.332 ± 0.027	5.271ArgPro: 5.271 ± 0.064	2.145ArgGln: 2.145 ± 0.029	7.774ArgArg: 7.774 ± 0.082	4.018ArgSer: 4.018 ± 0.039	5.685ArgThr: 5.685 ± 0.051	5.889ArgVal: 5.889 ± 0.051	1.396ArgTrp: 1.396 ± 0.025	1.827ArgTyr: 1.827 ± 0.026	0.001ArgXaa: 0.001 ± 0.001
Ser	6.532SerAla: 6.532 ± 0.058	0.406SerCys: 0.406 ± 0.013	2.506SerAsp: 2.506 ± 0.037	2.309SerGlu: 2.309 ± 0.034	1.516SerPhe: 1.516 ± 0.027	5.813SerGly: 5.813 ± 0.053	0.941SerHis: 0.941 ± 0.022	1.315SerIle: 1.315 ± 0.024	0.975SerLys: 0.975 ± 0.023	4.749SerLeu: 4.749 ± 0.049	1.033SerMet: 1.033 ± 0.023	0.809SerAsn: 0.809 ± 0.022	3.136SerPro: 3.136 ± 0.042	1.101SerGln: 1.101 ± 0.025	3.576SerArg: 3.576 ± 0.04	2.642SerSer: 2.642 ± 0.038	3.023SerThr: 3.023 ± 0.04	4.197SerVal: 4.197 ± 0.043	0.894SerTrp: 0.894 ± 0.02	1.204SerTyr: 1.204 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	9.416ThrAla: 9.416 ± 0.085	0.394ThrCys: 0.394 ± 0.013	3.66ThrAsp: 3.66 ± 0.042	3.455ThrGlu: 3.455 ± 0.044	1.611ThrPhe: 1.611 ± 0.028	7.214ThrGly: 7.214 ± 0.07	1.209ThrHis: 1.209 ± 0.022	1.627ThrIle: 1.627 ± 0.029	1.163ThrLys: 1.163 ± 0.028	5.813ThrLeu: 5.813 ± 0.057	0.912ThrMet: 0.912 ± 0.022	0.924ThrAsn: 0.924 ± 0.023	4.519ThrPro: 4.519 ± 0.051	1.274ThrGln: 1.274 ± 0.024	4.007ThrArg: 4.007 ± 0.033	3.122ThrSer: 3.122 ± 0.044	4.199ThrThr: 4.199 ± 0.061	6.228ThrVal: 6.228 ± 0.061	0.907ThrTrp: 0.907 ± 0.018	1.366ThrTyr: 1.366 ± 0.031	0.001ThrXaa: 0.001 ± 0.001
Val	10.777ValAla: 10.777 ± 0.069	0.7ValCys: 0.7 ± 0.016	4.968ValAsp: 4.968 ± 0.057	4.763ValGlu: 4.763 ± 0.049	2.393ValPhe: 2.393 ± 0.035	6.427ValGly: 6.427 ± 0.05	1.914ValHis: 1.914 ± 0.03	2.58ValIle: 2.58 ± 0.039	1.774ValLys: 1.774 ± 0.034	9.667ValLeu: 9.667 ± 0.081	1.388ValMet: 1.388 ± 0.025	1.577ValAsn: 1.577 ± 0.032	5.577ValPro: 5.577 ± 0.054	1.983ValGln: 1.983 ± 0.031	7.227ValArg: 7.227 ± 0.066	4.199ValSer: 4.199 ± 0.046	5.871ValThr: 5.871 ± 0.063	7.886ValVal: 7.886 ± 0.087	1.117ValTrp: 1.117 ± 0.022	1.566ValTyr: 1.566 ± 0.029	0.002ValXaa: 0.002 ± 0.001
Trp	1.677TrpAla: 1.677 ± 0.024	0.145TrpCys: 0.145 ± 0.009	0.799TrpAsp: 0.799 ± 0.019	0.767TrpGlu: 0.767 ± 0.017	0.5TrpPhe: 0.5 ± 0.015	1.086TrpGly: 1.086 ± 0.024	0.345TrpHis: 0.345 ± 0.012	0.584TrpIle: 0.584 ± 0.018	0.37TrpLys: 0.37 ± 0.012	1.737TrpLeu: 1.737 ± 0.031	0.289TrpMet: 0.289 ± 0.01	0.421TrpAsn: 0.421 ± 0.015	0.807TrpPro: 0.807 ± 0.018	0.595TrpGln: 0.595 ± 0.017	1.345TrpArg: 1.345 ± 0.027	0.947TrpSer: 0.947 ± 0.021	1.144TrpThr: 1.144 ± 0.025	1.0TrpVal: 1.0 ± 0.019	0.355TrpTrp: 0.355 ± 0.014	0.358TrpTyr: 0.358 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.835TyrAla: 2.835 ± 0.036	0.168TyrCys: 0.168 ± 0.008	1.496TyrAsp: 1.496 ± 0.028	1.323TyrGlu: 1.323 ± 0.023	0.608TyrPhe: 0.608 ± 0.016	2.389TyrGly: 2.389 ± 0.034	0.381TyrHis: 0.381 ± 0.012	0.45TyrIle: 0.45 ± 0.013	0.405TyrLys: 0.405 ± 0.015	2.02TyrLeu: 2.02 ± 0.029	0.241TyrMet: 0.241 ± 0.012	0.393TyrAsn: 0.393 ± 0.015	1.048TyrPro: 1.048 ± 0.021	0.598TyrGln: 0.598 ± 0.016	1.804TyrArg: 1.804 ± 0.025	0.882TyrSer: 0.882 ± 0.02	1.189TyrThr: 1.189 ± 0.026	1.645TyrVal: 1.645 ± 0.025	0.341TyrTrp: 0.341 ± 0.012	0.431TyrTyr: 0.431 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.09XaaXaa: 0.09 ± 0.027

Statistics based on 7451 proteins (2472419 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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