Amino acid dipepetide frequency for Synechococcus phage S-RIM2 R9_2006

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.57AlaAla: 6.57 ± 0.39
0.448AlaCys: 0.448 ± 0.11
4.189AlaAsp: 4.189 ± 0.29
4.028AlaGlu: 4.028 ± 0.266
3.168AlaPhe: 3.168 ± 0.245
6.767AlaGly: 6.767 ± 0.539
0.788AlaHis: 0.788 ± 0.096
4.207AlaIle: 4.207 ± 0.253
4.207AlaLys: 4.207 ± 0.352
4.78AlaLeu: 4.78 ± 0.289
1.522AlaMet: 1.522 ± 0.183
4.135AlaAsn: 4.135 ± 0.415
2.739AlaPro: 2.739 ± 0.206
2.667AlaGln: 2.667 ± 0.224
2.846AlaArg: 2.846 ± 0.274
4.994AlaSer: 4.994 ± 0.372
5.943AlaThr: 5.943 ± 0.558
4.994AlaVal: 4.994 ± 0.403
0.716AlaTrp: 0.716 ± 0.142
2.345AlaTyr: 2.345 ± 0.249
0.0AlaXaa: 0.0 ± 0.0
Cys
0.555CysAla: 0.555 ± 0.103
0.054CysCys: 0.054 ± 0.04
0.68CysAsp: 0.68 ± 0.127
0.537CysGlu: 0.537 ± 0.109
0.412CysPhe: 0.412 ± 0.095
0.68CysGly: 0.68 ± 0.142
0.215CysHis: 0.215 ± 0.073
0.501CysIle: 0.501 ± 0.118
0.752CysLys: 0.752 ± 0.148
0.644CysLeu: 0.644 ± 0.132
0.251CysMet: 0.251 ± 0.069
0.68CysAsn: 0.68 ± 0.154
0.322CysPro: 0.322 ± 0.084
0.43CysGln: 0.43 ± 0.081
0.358CysArg: 0.358 ± 0.087
0.591CysSer: 0.591 ± 0.12
0.483CysThr: 0.483 ± 0.099
0.627CysVal: 0.627 ± 0.123
0.125CysTrp: 0.125 ± 0.049
0.358CysTyr: 0.358 ± 0.073
0.0CysXaa: 0.0 ± 0.0
Asp
5.692AspAla: 5.692 ± 0.328
0.806AspCys: 0.806 ± 0.131
4.314AspAsp: 4.314 ± 0.259
3.741AspGlu: 3.741 ± 0.314
3.061AspPhe: 3.061 ± 0.216
5.8AspGly: 5.8 ± 0.442
0.949AspHis: 0.949 ± 0.16
4.332AspIle: 4.332 ± 0.303
3.473AspLys: 3.473 ± 0.296
4.475AspLeu: 4.475 ± 0.297
1.522AspMet: 1.522 ± 0.22
3.437AspAsn: 3.437 ± 0.258
3.312AspPro: 3.312 ± 0.261
2.166AspGln: 2.166 ± 0.173
2.721AspArg: 2.721 ± 0.253
4.296AspSer: 4.296 ± 0.296
4.475AspThr: 4.475 ± 0.313
4.314AspVal: 4.314 ± 0.258
1.11AspTrp: 1.11 ± 0.149
2.703AspTyr: 2.703 ± 0.219
0.0AspXaa: 0.0 ± 0.0
Glu
3.401GluAla: 3.401 ± 0.314
0.877GluCys: 0.877 ± 0.153
4.135GluAsp: 4.135 ± 0.305
4.797GluGlu: 4.797 ± 0.539
3.186GluPhe: 3.186 ± 0.246
4.028GluGly: 4.028 ± 0.298
1.02GluHis: 1.02 ± 0.191
3.67GluIle: 3.67 ± 0.271
3.67GluLys: 3.67 ± 0.395
5.102GluLeu: 5.102 ± 0.354
1.557GluMet: 1.557 ± 0.226
3.204GluAsn: 3.204 ± 0.266
1.611GluPro: 1.611 ± 0.157
2.399GluGln: 2.399 ± 0.192
2.918GluArg: 2.918 ± 0.332
3.688GluSer: 3.688 ± 0.299
4.135GluThr: 4.135 ± 0.303
4.636GluVal: 4.636 ± 0.248
0.823GluTrp: 0.823 ± 0.139
2.685GluTyr: 2.685 ± 0.231
0.0GluXaa: 0.0 ± 0.0
Phe
2.775PheAla: 2.775 ± 0.238
0.609PheCys: 0.609 ± 0.12
3.562PheAsp: 3.562 ± 0.263
2.757PheGlu: 2.757 ± 0.24
1.79PhePhe: 1.79 ± 0.173
3.133PheGly: 3.133 ± 0.226
0.501PheHis: 0.501 ± 0.102
2.542PheIle: 2.542 ± 0.24
2.649PheLys: 2.649 ± 0.238
2.739PheLeu: 2.739 ± 0.26
1.02PheMet: 1.02 ± 0.144
2.936PheAsn: 2.936 ± 0.241
1.754PhePro: 1.754 ± 0.21
1.736PheGln: 1.736 ± 0.169
1.522PheArg: 1.522 ± 0.168
3.007PheSer: 3.007 ± 0.231
2.972PheThr: 2.972 ± 0.258
2.649PheVal: 2.649 ± 0.298
0.304PheTrp: 0.304 ± 0.076
1.987PheTyr: 1.987 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
6.426GlyAla: 6.426 ± 0.516
0.627GlyCys: 0.627 ± 0.103
5.531GlyAsp: 5.531 ± 0.455
3.956GlyGlu: 3.956 ± 0.21
2.793GlyPhe: 2.793 ± 0.288
7.841GlyGly: 7.841 ± 0.808
1.343GlyHis: 1.343 ± 0.218
4.475GlyIle: 4.475 ± 0.328
3.795GlyLys: 3.795 ± 0.393
4.618GlyLeu: 4.618 ± 0.307
1.736GlyMet: 1.736 ± 0.25
4.851GlyAsn: 4.851 ± 0.605
2.041GlyPro: 2.041 ± 0.207
2.793GlyGln: 2.793 ± 0.227
2.846GlyArg: 2.846 ± 0.262
6.48GlySer: 6.48 ± 0.513
7.339GlyThr: 7.339 ± 0.732
5.066GlyVal: 5.066 ± 0.295
1.02GlyTrp: 1.02 ± 0.143
3.652GlyTyr: 3.652 ± 0.384
0.0GlyXaa: 0.0 ± 0.0
His
0.644HisAla: 0.644 ± 0.106
0.304HisCys: 0.304 ± 0.084
0.877HisAsp: 0.877 ± 0.141
0.949HisGlu: 0.949 ± 0.152
0.752HisPhe: 0.752 ± 0.128
1.11HisGly: 1.11 ± 0.189
0.286HisHis: 0.286 ± 0.094
0.949HisIle: 0.949 ± 0.132
0.806HisLys: 0.806 ± 0.17
1.002HisLeu: 1.002 ± 0.149
0.394HisMet: 0.394 ± 0.071
0.806HisAsn: 0.806 ± 0.135
0.895HisPro: 0.895 ± 0.164
0.483HisGln: 0.483 ± 0.091
0.573HisArg: 0.573 ± 0.106
0.806HisSer: 0.806 ± 0.109
1.02HisThr: 1.02 ± 0.146
0.913HisVal: 0.913 ± 0.183
0.215HisTrp: 0.215 ± 0.066
0.949HisTyr: 0.949 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
4.314IleAla: 4.314 ± 0.299
0.591IleCys: 0.591 ± 0.128
4.815IleAsp: 4.815 ± 0.266
4.189IleGlu: 4.189 ± 0.295
2.399IlePhe: 2.399 ± 0.208
4.171IleGly: 4.171 ± 0.317
0.555IleHis: 0.555 ± 0.112
3.365IleIle: 3.365 ± 0.273
3.831IleLys: 3.831 ± 0.268
4.046IleLeu: 4.046 ± 0.287
1.074IleMet: 1.074 ± 0.189
4.117IleAsn: 4.117 ± 0.29
2.506IlePro: 2.506 ± 0.278
2.542IleGln: 2.542 ± 0.209
2.381IleArg: 2.381 ± 0.189
4.117IleSer: 4.117 ± 0.377
5.531IleThr: 5.531 ± 0.589
3.849IleVal: 3.849 ± 0.354
0.609IleTrp: 0.609 ± 0.106
1.701IleTyr: 1.701 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
3.795LysAla: 3.795 ± 0.417
0.555LysCys: 0.555 ± 0.117
3.598LysAsp: 3.598 ± 0.347
3.849LysGlu: 3.849 ± 0.454
2.452LysPhe: 2.452 ± 0.252
3.168LysGly: 3.168 ± 0.28
0.931LysHis: 0.931 ± 0.203
3.741LysIle: 3.741 ± 0.274
4.547LysLys: 4.547 ± 0.727
4.565LysLeu: 4.565 ± 0.319
1.325LysMet: 1.325 ± 0.232
2.936LysAsn: 2.936 ± 0.258
1.772LysPro: 1.772 ± 0.178
2.077LysGln: 2.077 ± 0.257
2.452LysArg: 2.452 ± 0.297
3.795LysSer: 3.795 ± 0.341
3.419LysThr: 3.419 ± 0.236
4.099LysVal: 4.099 ± 0.3
0.841LysTrp: 0.841 ± 0.15
2.936LysTyr: 2.936 ± 0.335
0.0LysXaa: 0.0 ± 0.0
Leu
4.475LeuAla: 4.475 ± 0.293
0.716LeuCys: 0.716 ± 0.144
5.549LeuAsp: 5.549 ± 0.32
4.708LeuGlu: 4.708 ± 0.357
2.685LeuPhe: 2.685 ± 0.231
5.03LeuGly: 5.03 ± 0.315
1.164LeuHis: 1.164 ± 0.188
3.992LeuIle: 3.992 ± 0.269
4.422LeuLys: 4.422 ± 0.402
5.227LeuLeu: 5.227 ± 0.398
1.36LeuMet: 1.36 ± 0.223
4.636LeuAsn: 4.636 ± 0.351
2.846LeuPro: 2.846 ± 0.263
3.043LeuGln: 3.043 ± 0.23
3.347LeuArg: 3.347 ± 0.229
4.457LeuSer: 4.457 ± 0.306
5.692LeuThr: 5.692 ± 0.478
4.457LeuVal: 4.457 ± 0.283
0.716LeuTrp: 0.716 ± 0.111
3.115LeuTyr: 3.115 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
1.343MetAla: 1.343 ± 0.184
0.179MetCys: 0.179 ± 0.069
1.199MetAsp: 1.199 ± 0.151
1.378MetGlu: 1.378 ± 0.233
0.877MetPhe: 0.877 ± 0.163
1.253MetGly: 1.253 ± 0.194
0.483MetHis: 0.483 ± 0.112
1.146MetIle: 1.146 ± 0.16
1.378MetLys: 1.378 ± 0.252
1.593MetLeu: 1.593 ± 0.188
0.501MetMet: 0.501 ± 0.096
1.199MetAsn: 1.199 ± 0.194
0.895MetPro: 0.895 ± 0.157
0.931MetGln: 0.931 ± 0.16
1.002MetArg: 1.002 ± 0.168
1.915MetSer: 1.915 ± 0.228
1.36MetThr: 1.36 ± 0.204
1.343MetVal: 1.343 ± 0.167
0.233MetTrp: 0.233 ± 0.072
0.823MetTyr: 0.823 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
3.974AsnAla: 3.974 ± 0.319
0.43AsnCys: 0.43 ± 0.096
3.598AsnAsp: 3.598 ± 0.221
3.133AsnGlu: 3.133 ± 0.225
2.578AsnPhe: 2.578 ± 0.215
4.851AsnGly: 4.851 ± 0.364
0.698AsnHis: 0.698 ± 0.095
3.777AsnIle: 3.777 ± 0.384
3.151AsnLys: 3.151 ± 0.298
5.191AsnLeu: 5.191 ± 0.382
0.698AsnMet: 0.698 ± 0.126
3.562AsnAsn: 3.562 ± 0.318
2.954AsnPro: 2.954 ± 0.251
2.309AsnGln: 2.309 ± 0.185
2.238AsnArg: 2.238 ± 0.173
3.634AsnSer: 3.634 ± 0.297
4.493AsnThr: 4.493 ± 0.344
4.35AsnVal: 4.35 ± 0.357
0.662AsnTrp: 0.662 ± 0.103
2.381AsnTyr: 2.381 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
2.739ProAla: 2.739 ± 0.203
0.34ProCys: 0.34 ± 0.098
2.631ProAsp: 2.631 ± 0.291
2.667ProGlu: 2.667 ± 0.226
1.504ProPhe: 1.504 ± 0.202
3.204ProGly: 3.204 ± 0.299
0.644ProHis: 0.644 ± 0.1
2.309ProIle: 2.309 ± 0.215
1.969ProLys: 1.969 ± 0.236
2.22ProLeu: 2.22 ± 0.166
0.519ProMet: 0.519 ± 0.096
2.363ProAsn: 2.363 ± 0.188
1.575ProPro: 1.575 ± 0.146
1.378ProGln: 1.378 ± 0.153
1.45ProArg: 1.45 ± 0.171
3.079ProSer: 3.079 ± 0.229
2.864ProThr: 2.864 ± 0.235
2.542ProVal: 2.542 ± 0.231
0.591ProTrp: 0.591 ± 0.102
1.522ProTyr: 1.522 ± 0.188
0.0ProXaa: 0.0 ± 0.0
Gln
2.47GlnAla: 2.47 ± 0.184
0.322GlnCys: 0.322 ± 0.09
2.166GlnAsp: 2.166 ± 0.182
2.524GlnGlu: 2.524 ± 0.262
1.593GlnPhe: 1.593 ± 0.168
2.506GlnGly: 2.506 ± 0.227
0.806GlnHis: 0.806 ± 0.137
2.524GlnIle: 2.524 ± 0.213
2.202GlnLys: 2.202 ± 0.265
2.936GlnLeu: 2.936 ± 0.249
0.949GlnMet: 0.949 ± 0.163
2.005GlnAsn: 2.005 ± 0.183
1.36GlnPro: 1.36 ± 0.155
1.736GlnGln: 1.736 ± 0.205
1.79GlnArg: 1.79 ± 0.164
2.291GlnSer: 2.291 ± 0.192
2.273GlnThr: 2.273 ± 0.198
2.9GlnVal: 2.9 ± 0.242
0.644GlnTrp: 0.644 ± 0.108
1.701GlnTyr: 1.701 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
2.757ArgAla: 2.757 ± 0.212
0.251ArgCys: 0.251 ± 0.065
2.273ArgAsp: 2.273 ± 0.198
2.56ArgGlu: 2.56 ± 0.299
2.059ArgPhe: 2.059 ± 0.174
2.972ArgGly: 2.972 ± 0.31
0.716ArgHis: 0.716 ± 0.148
2.846ArgIle: 2.846 ± 0.225
2.667ArgLys: 2.667 ± 0.359
3.419ArgLeu: 3.419 ± 0.198
1.074ArgMet: 1.074 ± 0.201
2.166ArgAsn: 2.166 ± 0.186
1.36ArgPro: 1.36 ± 0.148
1.629ArgGln: 1.629 ± 0.167
1.933ArgArg: 1.933 ± 0.266
2.345ArgSer: 2.345 ± 0.244
2.488ArgThr: 2.488 ± 0.271
3.007ArgVal: 3.007 ± 0.208
0.448ArgTrp: 0.448 ± 0.095
2.327ArgTyr: 2.327 ± 0.247
0.0ArgXaa: 0.0 ± 0.0
Ser
5.907SerAla: 5.907 ± 0.373
0.537SerCys: 0.537 ± 0.121
3.992SerAsp: 3.992 ± 0.24
3.777SerGlu: 3.777 ± 0.265
3.204SerPhe: 3.204 ± 0.358
7.16SerGly: 7.16 ± 0.489
0.931SerHis: 0.931 ± 0.125
3.885SerIle: 3.885 ± 0.456
3.473SerLys: 3.473 ± 0.294
4.69SerLeu: 4.69 ± 0.243
1.629SerMet: 1.629 ± 0.279
4.189SerAsn: 4.189 ± 0.376
2.56SerPro: 2.56 ± 0.261
2.166SerGln: 2.166 ± 0.237
2.345SerArg: 2.345 ± 0.232
5.531SerSer: 5.531 ± 0.612
5.173SerThr: 5.173 ± 0.411
4.332SerVal: 4.332 ± 0.397
0.68SerTrp: 0.68 ± 0.088
2.685SerTyr: 2.685 ± 0.208
0.0SerXaa: 0.0 ± 0.0
Thr
6.265ThrAla: 6.265 ± 0.524
0.34ThrCys: 0.34 ± 0.085
4.511ThrAsp: 4.511 ± 0.35
4.225ThrGlu: 4.225 ± 0.373
3.294ThrPhe: 3.294 ± 0.288
7.393ThrGly: 7.393 ± 0.924
0.913ThrHis: 0.913 ± 0.13
5.03ThrIle: 5.03 ± 0.457
3.186ThrLys: 3.186 ± 0.265
6.283ThrLeu: 6.283 ± 0.377
1.217ThrMet: 1.217 ± 0.149
4.028ThrAsn: 4.028 ± 0.387
3.061ThrPro: 3.061 ± 0.254
2.649ThrGln: 2.649 ± 0.201
2.775ThrArg: 2.775 ± 0.225
5.424ThrSer: 5.424 ± 0.697
6.409ThrThr: 6.409 ± 0.557
6.033ThrVal: 6.033 ± 0.622
0.734ThrTrp: 0.734 ± 0.126
2.9ThrTyr: 2.9 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
4.797ValAla: 4.797 ± 0.304
0.537ValCys: 0.537 ± 0.108
5.209ValAsp: 5.209 ± 0.363
4.457ValGlu: 4.457 ± 0.288
2.864ValPhe: 2.864 ± 0.215
5.12ValGly: 5.12 ± 0.643
0.841ValHis: 0.841 ± 0.11
4.064ValIle: 4.064 ± 0.311
3.705ValLys: 3.705 ± 0.291
4.046ValLeu: 4.046 ± 0.28
1.378ValMet: 1.378 ± 0.184
4.064ValAsn: 4.064 ± 0.327
2.882ValPro: 2.882 ± 0.256
2.452ValGln: 2.452 ± 0.197
2.989ValArg: 2.989 ± 0.232
4.905ValSer: 4.905 ± 0.377
6.373ValThr: 6.373 ± 0.617
4.654ValVal: 4.654 ± 0.312
0.68ValTrp: 0.68 ± 0.101
2.596ValTyr: 2.596 ± 0.246
0.0ValXaa: 0.0 ± 0.0
Trp
0.806TrpAla: 0.806 ± 0.107
0.161TrpCys: 0.161 ± 0.063
0.823TrpAsp: 0.823 ± 0.153
0.662TrpGlu: 0.662 ± 0.139
0.465TrpPhe: 0.465 ± 0.103
0.752TrpGly: 0.752 ± 0.124
0.394TrpHis: 0.394 ± 0.096
0.662TrpIle: 0.662 ± 0.117
0.788TrpLys: 0.788 ± 0.154
0.806TrpLeu: 0.806 ± 0.152
0.286TrpMet: 0.286 ± 0.076
0.877TrpAsn: 0.877 ± 0.142
0.143TrpPro: 0.143 ± 0.055
0.465TrpGln: 0.465 ± 0.074
0.501TrpArg: 0.501 ± 0.089
0.806TrpSer: 0.806 ± 0.126
0.931TrpThr: 0.931 ± 0.118
0.788TrpVal: 0.788 ± 0.122
0.161TrpTrp: 0.161 ± 0.055
0.501TrpTyr: 0.501 ± 0.077
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.345TyrAla: 2.345 ± 0.17
0.573TyrCys: 0.573 ± 0.104
3.043TyrAsp: 3.043 ± 0.232
2.649TyrGlu: 2.649 ± 0.264
1.808TyrPhe: 1.808 ± 0.183
2.291TyrGly: 2.291 ± 0.178
0.555TyrHis: 0.555 ± 0.104
2.685TyrIle: 2.685 ± 0.228
2.184TyrLys: 2.184 ± 0.27
3.258TyrLeu: 3.258 ± 0.256
1.002TyrMet: 1.002 ± 0.16
2.47TyrAsn: 2.47 ± 0.243
1.522TyrPro: 1.522 ± 0.159
1.647TyrGln: 1.647 ± 0.169
2.309TyrArg: 2.309 ± 0.202
2.685TyrSer: 2.685 ± 0.262
3.294TyrThr: 3.294 ± 0.37
3.079TyrVal: 3.079 ± 0.235
0.465TyrTrp: 0.465 ± 0.106
1.772TyrTyr: 1.772 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 210 proteins (55864 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski