Mangrovimonas sp. DI 80

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mangrovimonas; unclassified Mangrovimonas

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V1ZJV8|A0A1V1ZJV8_9FLAO Sialidase domain-containing protein OS=Mangrovimonas sp. DI 80 OX=1779330 GN=BKM32_04820 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84KK3 pKa = 9.65FSFQLLVLSLVLFAVGCSNDD23 pKa = 3.26SSDD26 pKa = 3.71VEE28 pKa = 4.57EE29 pKa = 4.48IQVTSITILGEE40 pKa = 4.22TISDD44 pKa = 3.76GLSSQLSVQIVPANATEE61 pKa = 4.36TSVTWSVSDD70 pKa = 3.54PTVATISNNGLLSAVSNGSVNVTATSQDD98 pKa = 3.03GSGVFGDD105 pKa = 3.86RR106 pKa = 11.84SFTISGVGVGPVIVVQTSQEE126 pKa = 3.67ITEE129 pKa = 4.81AIITASAGDD138 pKa = 3.93IIYY141 pKa = 10.8VKK143 pKa = 10.57GGEE146 pKa = 4.06YY147 pKa = 10.48QFASTINFSADD158 pKa = 3.08GTSEE162 pKa = 3.74NAIIFMGDD170 pKa = 3.21PDD172 pKa = 4.52DD173 pKa = 4.91SEE175 pKa = 5.37RR176 pKa = 11.84PQFNFSAMSEE186 pKa = 4.04NSSNRR191 pKa = 11.84GIQLSGDD198 pKa = 3.16YY199 pKa = 9.18WHH201 pKa = 7.49IKK203 pKa = 9.74GIDD206 pKa = 3.36VFGAGDD212 pKa = 3.45NGMYY216 pKa = 8.7ITGHH220 pKa = 5.51YY221 pKa = 10.21NLVEE225 pKa = 4.06FCTFSEE231 pKa = 4.38NADD234 pKa = 3.41SGLQISGGGSNNTILNCDD252 pKa = 3.07SFYY255 pKa = 11.34NADD258 pKa = 3.7SSLEE262 pKa = 4.04NADD265 pKa = 4.03GFACKK270 pKa = 9.11LTAGTDD276 pKa = 3.34NKK278 pKa = 10.68FIGCRR283 pKa = 11.84AWQNLDD289 pKa = 4.34DD290 pKa = 4.74GWDD293 pKa = 3.59GYY295 pKa = 11.67LRR297 pKa = 11.84DD298 pKa = 4.16NDD300 pKa = 4.62NITTYY305 pKa = 11.25YY306 pKa = 8.36EE307 pKa = 3.95NCWAFKK313 pKa = 10.51NGYY316 pKa = 10.17LMDD319 pKa = 4.64GSAGIGDD326 pKa = 3.84GNGFKK331 pKa = 10.23TGGSDD336 pKa = 4.36DD337 pKa = 3.81KK338 pKa = 10.88TLKK341 pKa = 10.1HH342 pKa = 6.1HH343 pKa = 6.78GVYY346 pKa = 10.28KK347 pKa = 10.71NCIAAGNIYY356 pKa = 10.62DD357 pKa = 4.78GFDD360 pKa = 3.38HH361 pKa = 6.81NSNRR365 pKa = 11.84GDD367 pKa = 3.34IEE369 pKa = 4.89LYY371 pKa = 10.58NCSAYY376 pKa = 11.18NNGRR380 pKa = 11.84NISFSSTNIANFLLIKK396 pKa = 10.61NSLSFEE402 pKa = 4.46GNNSDD407 pKa = 5.01SYY409 pKa = 11.73SATQTDD415 pKa = 3.15ISNNGWQSGLTTDD428 pKa = 4.2ASDD431 pKa = 4.25FVSLDD436 pKa = 3.22MDD438 pKa = 4.66LLASPRR444 pKa = 11.84NADD447 pKa = 3.32GSLPDD452 pKa = 3.77IDD454 pKa = 4.65FLKK457 pKa = 10.76LSSGSDD463 pKa = 3.78LIDD466 pKa = 3.25QGVDD470 pKa = 2.82VGLPFLGTAPDD481 pKa = 3.3IGAFEE486 pKa = 4.18YY487 pKa = 10.9QDD489 pKa = 3.15

Molecular weight: 52.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V1ZGM4|A0A1V1ZGM4_9FLAO RNA-binding protein OS=Mangrovimonas sp. DI 80 OX=1779330 GN=BKM32_09690 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.52LSVSSEE48 pKa = 3.87PRR50 pKa = 11.84HH51 pKa = 5.92KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3194

0

3194

1127307

30

3754

352.9

39.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.531 ± 0.044

0.789 ± 0.015

5.679 ± 0.029

6.762 ± 0.038

5.156 ± 0.033

6.531 ± 0.04

1.891 ± 0.02

7.418 ± 0.04

7.109 ± 0.057

9.384 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.023

5.916 ± 0.045

3.554 ± 0.026

3.504 ± 0.024

3.297 ± 0.03

6.507 ± 0.036

5.915 ± 0.06

6.436 ± 0.035

1.091 ± 0.016

4.232 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski