Vibrio xiamenensis
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4859 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8FFI7|A0A1G8FFI7_9VIBR Integrating conjugative element membrane protein PFL_4697 family OS=Vibrio xiamenensis OX=861298 GN=SAMN04488136_13033 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.33 KK3 pKa = 9.67 ILPILAGTMALTTFSTLSHH22 pKa = 6.53 AAVLDD27 pKa = 4.2 DD28 pKa = 3.95 EE29 pKa = 5.1 SGVYY33 pKa = 10.14 VGGNYY38 pKa = 10.48 GYY40 pKa = 11.25 VKK42 pKa = 10.7 VDD44 pKa = 3.45 GQDD47 pKa = 5.18 DD48 pKa = 4.0 FDD50 pKa = 6.83 DD51 pKa = 5.77 DD52 pKa = 4.65 NDD54 pKa = 3.74 VMQALIGFKK63 pKa = 9.79 LNRR66 pKa = 11.84 YY67 pKa = 8.8 IGVEE71 pKa = 3.67 GSYY74 pKa = 10.52 IDD76 pKa = 3.6 FGSYY80 pKa = 9.9 GGSVASAEE88 pKa = 4.26 TDD90 pKa = 3.5 GYY92 pKa = 9.19 TAALKK97 pKa = 8.95 LTAPIGDD104 pKa = 3.45 RR105 pKa = 11.84 VEE107 pKa = 5.4 LYY109 pKa = 11.01 AKK111 pKa = 10.33 GGQLWYY117 pKa = 7.98 TTDD120 pKa = 3.3 YY121 pKa = 11.29 DD122 pKa = 3.52 IAGFNGDD129 pKa = 4.04 EE130 pKa = 4.88 DD131 pKa = 4.07 DD132 pKa = 3.95 TAVFAGAGVGFKK144 pKa = 9.6 VTDD147 pKa = 3.39 NFLINAEE154 pKa = 4.22 YY155 pKa = 10.48 AWYY158 pKa = 9.98 DD159 pKa = 3.07 VDD161 pKa = 6.46 LDD163 pKa = 4.63 ANDD166 pKa = 4.21 INDD169 pKa = 4.32 GADD172 pKa = 3.03 TNTDD176 pKa = 4.07 FKK178 pKa = 11.1 QATIGAEE185 pKa = 3.86 YY186 pKa = 10.66 RR187 pKa = 11.84 FF188 pKa = 3.94
Molecular weight: 20.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.516
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.745
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.592
EMBOSS 3.745
Sillero 3.884
Patrickios 1.1
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A1G8FRL2|A0A1G8FRL2_9VIBR Phosphoadenosine phosphosulfate reductase OS=Vibrio xiamenensis OX=861298 GN=cysH PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 NGRR28 pKa = 11.84 KK29 pKa = 9.39 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSKK44 pKa = 10.84
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4859
0
4859
1549248
11
3731
318.8
35.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.857 ± 0.035
1.064 ± 0.013
5.607 ± 0.032
5.938 ± 0.033
4.092 ± 0.025
6.721 ± 0.032
2.334 ± 0.021
6.176 ± 0.025
5.0 ± 0.027
10.39 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.652 ± 0.019
4.138 ± 0.024
3.901 ± 0.024
4.934 ± 0.031
4.549 ± 0.027
6.874 ± 0.041
5.277 ± 0.031
7.081 ± 0.029
1.267 ± 0.012
3.147 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here