Bacillus phage vB_BsuP-Goe1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Picovirinae; Beecentumtrevirus; Bacillus virus Goe1

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142IG80|A0A142IG80_9CAUD Uncharacterized protein OS=Bacillus phage vB_BsuP-Goe1 OX=1807511 GN=Goe1_c00050 PE=4 SV=1
MM1 pKa = 7.22LQNDD5 pKa = 5.26FIDD8 pKa = 4.32SYY10 pKa = 10.1TLSWLLRR17 pKa = 11.84DD18 pKa = 4.52DD19 pKa = 3.95VGSEE23 pKa = 3.36HH24 pKa = 6.68WEE26 pKa = 3.86VHH28 pKa = 5.7EE29 pKa = 4.76GLSLSDD35 pKa = 3.73FEE37 pKa = 5.14VVYY40 pKa = 10.97GNNPHH45 pKa = 6.84QIVKK49 pKa = 10.53LNLVKK54 pKa = 10.67EE55 pKa = 4.28II56 pKa = 4.23

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142IG85|A0A142IG85_9CAUD Single-stranded DNA binding protein OS=Bacillus phage vB_BsuP-Goe1 OX=1807511 GN=Goe1_c00110 PE=4 SV=1
MM1 pKa = 7.62PRR3 pKa = 11.84TARR6 pKa = 11.84GIYY9 pKa = 9.96HH10 pKa = 6.27NLKK13 pKa = 8.42EE14 pKa = 4.23SEE16 pKa = 4.48YY17 pKa = 10.71VVSNGDD23 pKa = 2.98ATFFFSSEE31 pKa = 3.72MYY33 pKa = 10.69LNKK36 pKa = 10.01FLDD39 pKa = 5.18GYY41 pKa = 9.41QKK43 pKa = 10.49HH44 pKa = 5.97RR45 pKa = 11.84EE46 pKa = 3.99EE47 pKa = 4.3FNKK50 pKa = 10.25KK51 pKa = 9.03INRR54 pKa = 11.84ITDD57 pKa = 3.41TPLNMDD63 pKa = 4.26MLADD67 pKa = 3.16ITFYY71 pKa = 11.59SNVEE75 pKa = 3.69KK76 pKa = 10.74RR77 pKa = 11.84GFHH80 pKa = 6.44AWLKK84 pKa = 10.02GCNTTWQEE92 pKa = 3.37IHH94 pKa = 6.75VYY96 pKa = 10.57ALRR99 pKa = 11.84TMTKK103 pKa = 9.48PCTQNWSRR111 pKa = 11.84IRR113 pKa = 11.84RR114 pKa = 11.84PKK116 pKa = 9.91LVEE119 pKa = 3.68RR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 11.12SMVV125 pKa = 2.71

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

5767

36

861

240.3

27.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.948 ± 0.526

0.798 ± 0.179

6.069 ± 0.336

6.641 ± 0.611

4.04 ± 0.41

6.624 ± 0.901

1.561 ± 0.151

6.936 ± 0.401

7.664 ± 0.757

7.612 ± 0.534

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.235

7.127 ± 0.583

2.809 ± 0.209

3.225 ± 0.295

3.919 ± 0.394

6.589 ± 0.559

6.901 ± 0.567

6.867 ± 0.534

1.179 ± 0.208

4.769 ± 0.391
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski