Moraxella phage Mcat9
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHN8|A0A0R6PHN8_9CAUD Uncharacterized protein OS=Moraxella phage Mcat9 OX=1647555 PE=4 SV=1
MM1 pKa = 7.1 NASEE5 pKa = 5.54 IIYY8 pKa = 8.19 QTLSQIVDD16 pKa = 3.55 GKK18 pKa = 10.85 VYY20 pKa = 10.26 PLLVPEE26 pKa = 4.74 SDD28 pKa = 3.96 SLSVPYY34 pKa = 10.24 LVYY37 pKa = 10.33 TPVSSVPVQTLDD49 pKa = 4.75 GISGDD54 pKa = 2.92 EE55 pKa = 4.05 WVRR58 pKa = 11.84 VQIDD62 pKa = 4.16 IYY64 pKa = 11.03 HH65 pKa = 6.55 SDD67 pKa = 3.76 YY68 pKa = 11.63 DD69 pKa = 4.12 VLLSLYY75 pKa = 9.67 NQVISALQNKK85 pKa = 9.46 ISLKK89 pKa = 10.69 VFGTSNQSVDD99 pKa = 3.41 DD100 pKa = 3.82 GLYY103 pKa = 10.91 RR104 pKa = 11.84 MMFEE108 pKa = 4.81 CEE110 pKa = 3.87 FWSKK114 pKa = 10.6 NQILPTAPP122 pKa = 3.3
Molecular weight: 13.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.933
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.215
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.037
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 0.604
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.058
Protein with the highest isoelectric point:
>tr|A0A0R6PHR7|A0A0R6PHR7_9CAUD Uncharacterized protein OS=Moraxella phage Mcat9 OX=1647555 PE=4 SV=1
MM1 pKa = 7.45 AVLAGKK7 pKa = 10.15 QITQQRR13 pKa = 11.84 FVTVTTPRR21 pKa = 11.84 TTTTTIAPINSGFRR35 pKa = 11.84 LVAAA39 pKa = 5.31
Molecular weight: 4.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 10.555
IPC_protein 12.149
Toseland 12.31
ProMoST 12.808
Dawson 12.31
Bjellqvist 12.31
Wikipedia 12.778
Rodwell 11.974
Grimsley 12.354
Solomon 12.808
Lehninger 12.705
Nozaki 12.31
DTASelect 12.31
Thurlkill 12.31
EMBOSS 12.808
Sillero 12.31
Patrickios 11.798
IPC_peptide 12.808
IPC2_peptide 11.798
IPC2.peptide.svr19 9.18
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
42
0
42
11041
39
1474
262.9
29.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.967 ± 0.686
0.879 ± 0.172
6.44 ± 0.291
5.126 ± 0.285
3.505 ± 0.235
6.684 ± 0.323
2.246 ± 0.273
6.757 ± 0.235
6.594 ± 0.37
8.704 ± 0.338
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.329
5.072 ± 0.327
3.478 ± 0.323
4.864 ± 0.318
4.601 ± 0.269
6.376 ± 0.415
6.702 ± 0.467
6.086 ± 0.272
1.196 ± 0.114
3.261 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here