Amino acid dipepetide frequency for Pseudomonas phage phiNV3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.4AlaAla: 16.4 ± 2.185
1.166AlaCys: 1.166 ± 0.3
6.529AlaAsp: 6.529 ± 0.785
7.462AlaGlu: 7.462 ± 0.815
3.575AlaPhe: 3.575 ± 0.679
10.493AlaGly: 10.493 ± 0.832
1.788AlaHis: 1.788 ± 0.312
4.663AlaIle: 4.663 ± 0.709
5.907AlaLys: 5.907 ± 0.757
11.581AlaLeu: 11.581 ± 1.09
2.72AlaMet: 2.72 ± 0.421
3.42AlaAsn: 3.42 ± 0.491
4.119AlaPro: 4.119 ± 0.978
5.596AlaGln: 5.596 ± 0.686
4.663AlaArg: 4.663 ± 0.677
5.285AlaSer: 5.285 ± 0.725
6.296AlaThr: 6.296 ± 0.897
8.161AlaVal: 8.161 ± 0.941
0.933AlaTrp: 0.933 ± 0.23
3.42AlaTyr: 3.42 ± 0.583
0.0AlaXaa: 0.0 ± 0.0
Cys
0.933CysAla: 0.933 ± 0.422
0.155CysCys: 0.155 ± 0.116
0.855CysAsp: 0.855 ± 0.257
0.466CysGlu: 0.466 ± 0.217
0.233CysPhe: 0.233 ± 0.167
0.466CysGly: 0.466 ± 0.204
0.155CysHis: 0.155 ± 0.113
0.777CysIle: 0.777 ± 0.229
0.311CysLys: 0.311 ± 0.173
1.554CysLeu: 1.554 ± 0.426
0.544CysMet: 0.544 ± 0.21
0.389CysAsn: 0.389 ± 0.186
0.777CysPro: 0.777 ± 0.298
0.466CysGln: 0.466 ± 0.193
0.622CysArg: 0.622 ± 0.21
0.466CysSer: 0.466 ± 0.227
0.777CysThr: 0.777 ± 0.307
0.622CysVal: 0.622 ± 0.221
0.389CysTrp: 0.389 ± 0.162
0.466CysTyr: 0.466 ± 0.224
0.0CysXaa: 0.0 ± 0.0
Asp
6.917AspAla: 6.917 ± 0.679
0.466AspCys: 0.466 ± 0.164
3.653AspAsp: 3.653 ± 0.588
2.72AspGlu: 2.72 ± 0.622
2.254AspPhe: 2.254 ± 0.361
4.819AspGly: 4.819 ± 0.525
1.321AspHis: 1.321 ± 0.402
3.187AspIle: 3.187 ± 0.352
2.487AspLys: 2.487 ± 0.352
5.596AspLeu: 5.596 ± 0.734
2.487AspMet: 2.487 ± 0.395
1.477AspAsn: 1.477 ± 0.249
3.731AspPro: 3.731 ± 0.666
1.865AspGln: 1.865 ± 0.345
4.119AspArg: 4.119 ± 0.602
3.031AspSer: 3.031 ± 0.438
4.197AspThr: 4.197 ± 0.523
3.264AspVal: 3.264 ± 0.426
1.166AspTrp: 1.166 ± 0.386
1.477AspTyr: 1.477 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
7.772GluAla: 7.772 ± 0.926
0.7GluCys: 0.7 ± 0.256
2.643GluAsp: 2.643 ± 0.397
2.876GluGlu: 2.876 ± 0.554
2.72GluPhe: 2.72 ± 0.46
3.653GluGly: 3.653 ± 0.5
1.477GluHis: 1.477 ± 0.386
2.099GluIle: 2.099 ± 0.512
2.565GluLys: 2.565 ± 0.725
6.14GluLeu: 6.14 ± 0.559
2.176GluMet: 2.176 ± 0.413
1.788GluAsn: 1.788 ± 0.415
1.321GluPro: 1.321 ± 0.303
3.42GluGln: 3.42 ± 0.582
3.109GluArg: 3.109 ± 0.476
2.643GluSer: 2.643 ± 0.4
2.565GluThr: 2.565 ± 0.408
3.731GluVal: 3.731 ± 0.585
0.777GluTrp: 0.777 ± 0.273
2.254GluTyr: 2.254 ± 0.469
0.0GluXaa: 0.0 ± 0.0
Phe
3.109PheAla: 3.109 ± 0.545
0.389PheCys: 0.389 ± 0.15
2.798PheAsp: 2.798 ± 0.669
2.332PheGlu: 2.332 ± 0.382
0.777PhePhe: 0.777 ± 0.24
2.254PheGly: 2.254 ± 0.391
0.544PheHis: 0.544 ± 0.213
1.321PheIle: 1.321 ± 0.255
1.01PheLys: 1.01 ± 0.277
2.876PheLeu: 2.876 ± 0.531
1.166PheMet: 1.166 ± 0.322
2.021PheAsn: 2.021 ± 0.417
1.321PhePro: 1.321 ± 0.293
1.865PheGln: 1.865 ± 0.473
1.632PheArg: 1.632 ± 0.335
1.788PheSer: 1.788 ± 0.342
1.788PheThr: 1.788 ± 0.394
2.021PheVal: 2.021 ± 0.327
0.389PheTrp: 0.389 ± 0.159
0.855PheTyr: 0.855 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
7.928GlyAla: 7.928 ± 0.853
0.933GlyCys: 0.933 ± 0.286
4.042GlyAsp: 4.042 ± 0.548
3.964GlyGlu: 3.964 ± 0.545
2.565GlyPhe: 2.565 ± 0.457
7.151GlyGly: 7.151 ± 0.771
1.01GlyHis: 1.01 ± 0.268
4.741GlyIle: 4.741 ± 0.525
5.596GlyLys: 5.596 ± 0.656
7.306GlyLeu: 7.306 ± 0.719
2.176GlyMet: 2.176 ± 0.508
2.643GlyAsn: 2.643 ± 0.55
2.176GlyPro: 2.176 ± 0.452
4.43GlyGln: 4.43 ± 0.699
5.285GlyArg: 5.285 ± 0.645
4.197GlySer: 4.197 ± 0.664
6.373GlyThr: 6.373 ± 0.639
6.684GlyVal: 6.684 ± 0.755
1.088GlyTrp: 1.088 ± 0.288
3.031GlyTyr: 3.031 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
1.788HisAla: 1.788 ± 0.32
0.078HisCys: 0.078 ± 0.066
1.088HisAsp: 1.088 ± 0.311
1.399HisGlu: 1.399 ± 0.3
0.622HisPhe: 0.622 ± 0.22
1.865HisGly: 1.865 ± 0.364
0.389HisHis: 0.389 ± 0.193
1.244HisIle: 1.244 ± 0.299
1.01HisLys: 1.01 ± 0.253
1.788HisLeu: 1.788 ± 0.369
0.7HisMet: 0.7 ± 0.249
0.777HisAsn: 0.777 ± 0.199
1.088HisPro: 1.088 ± 0.234
0.777HisGln: 0.777 ± 0.23
1.554HisArg: 1.554 ± 0.353
1.01HisSer: 1.01 ± 0.298
0.933HisThr: 0.933 ± 0.261
1.088HisVal: 1.088 ± 0.293
0.233HisTrp: 0.233 ± 0.154
0.389HisTyr: 0.389 ± 0.176
0.0HisXaa: 0.0 ± 0.0
Ile
4.042IleAla: 4.042 ± 0.541
0.389IleCys: 0.389 ± 0.154
3.264IleAsp: 3.264 ± 0.46
2.409IleGlu: 2.409 ± 0.385
0.466IlePhe: 0.466 ± 0.256
2.798IleGly: 2.798 ± 0.445
1.166IleHis: 1.166 ± 0.317
1.477IleIle: 1.477 ± 0.305
3.187IleLys: 3.187 ± 0.606
2.643IleLeu: 2.643 ± 0.416
1.244IleMet: 1.244 ± 0.303
1.788IleAsn: 1.788 ± 0.246
2.254IlePro: 2.254 ± 0.398
1.477IleGln: 1.477 ± 0.272
2.876IleArg: 2.876 ± 0.468
2.409IleSer: 2.409 ± 0.353
2.876IleThr: 2.876 ± 0.665
3.498IleVal: 3.498 ± 0.623
0.622IleTrp: 0.622 ± 0.219
0.855IleTyr: 0.855 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
6.062LysAla: 6.062 ± 0.775
0.311LysCys: 0.311 ± 0.146
3.498LysAsp: 3.498 ± 0.666
1.943LysGlu: 1.943 ± 0.363
2.487LysPhe: 2.487 ± 0.494
4.275LysGly: 4.275 ± 0.583
1.088LysHis: 1.088 ± 0.346
1.865LysIle: 1.865 ± 0.423
1.943LysLys: 1.943 ± 0.417
5.363LysLeu: 5.363 ± 0.597
0.855LysMet: 0.855 ± 0.265
1.399LysAsn: 1.399 ± 0.341
2.72LysPro: 2.72 ± 0.591
2.409LysGln: 2.409 ± 0.556
3.42LysArg: 3.42 ± 0.439
2.099LysSer: 2.099 ± 0.393
1.71LysThr: 1.71 ± 0.382
3.575LysVal: 3.575 ± 0.548
0.7LysTrp: 0.7 ± 0.222
1.166LysTyr: 1.166 ± 0.288
0.0LysXaa: 0.0 ± 0.0
Leu
8.55LeuAla: 8.55 ± 0.92
1.321LeuCys: 1.321 ± 0.365
6.917LeuAsp: 6.917 ± 0.804
4.663LeuGlu: 4.663 ± 0.632
2.409LeuPhe: 2.409 ± 0.358
7.617LeuGly: 7.617 ± 1.014
2.332LeuHis: 2.332 ± 0.482
2.954LeuIle: 2.954 ± 0.536
4.275LeuLys: 4.275 ± 0.495
7.462LeuLeu: 7.462 ± 0.785
2.565LeuMet: 2.565 ± 0.38
3.575LeuAsn: 3.575 ± 0.675
4.43LeuPro: 4.43 ± 0.808
3.031LeuGln: 3.031 ± 0.571
7.384LeuArg: 7.384 ± 0.692
5.13LeuSer: 5.13 ± 0.602
6.529LeuThr: 6.529 ± 0.547
5.285LeuVal: 5.285 ± 0.611
1.088LeuTrp: 1.088 ± 0.292
2.565LeuTyr: 2.565 ± 0.37
0.0LeuXaa: 0.0 ± 0.0
Met
3.264MetAla: 3.264 ± 0.481
0.233MetCys: 0.233 ± 0.114
1.477MetAsp: 1.477 ± 0.32
1.943MetGlu: 1.943 ± 0.429
0.7MetPhe: 0.7 ± 0.261
2.021MetGly: 2.021 ± 0.455
0.622MetHis: 0.622 ± 0.183
0.777MetIle: 0.777 ± 0.217
1.244MetLys: 1.244 ± 0.345
2.876MetLeu: 2.876 ± 0.377
0.466MetMet: 0.466 ± 0.217
1.244MetAsn: 1.244 ± 0.323
1.244MetPro: 1.244 ± 0.324
1.71MetGln: 1.71 ± 0.374
2.099MetArg: 2.099 ± 0.364
2.487MetSer: 2.487 ± 0.537
1.632MetThr: 1.632 ± 0.301
1.477MetVal: 1.477 ± 0.352
0.311MetTrp: 0.311 ± 0.152
1.088MetTyr: 1.088 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
3.886AsnAla: 3.886 ± 0.63
0.311AsnCys: 0.311 ± 0.161
1.788AsnAsp: 1.788 ± 0.433
1.477AsnGlu: 1.477 ± 0.312
1.01AsnPhe: 1.01 ± 0.221
3.653AsnGly: 3.653 ± 0.628
0.544AsnHis: 0.544 ± 0.254
1.244AsnIle: 1.244 ± 0.416
2.254AsnLys: 2.254 ± 0.435
2.798AsnLeu: 2.798 ± 0.403
1.088AsnMet: 1.088 ± 0.333
1.477AsnAsn: 1.477 ± 0.513
1.71AsnPro: 1.71 ± 0.358
1.554AsnGln: 1.554 ± 0.303
1.788AsnArg: 1.788 ± 0.316
1.321AsnSer: 1.321 ± 0.263
2.643AsnThr: 2.643 ± 0.472
2.72AsnVal: 2.72 ± 0.552
0.855AsnTrp: 0.855 ± 0.244
0.855AsnTyr: 0.855 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
7.384ProAla: 7.384 ± 1.458
0.389ProCys: 0.389 ± 0.162
3.109ProAsp: 3.109 ± 0.474
4.042ProGlu: 4.042 ± 0.48
0.933ProPhe: 0.933 ± 0.246
3.808ProGly: 3.808 ± 0.587
1.01ProHis: 1.01 ± 0.213
1.71ProIle: 1.71 ± 0.344
2.254ProLys: 2.254 ± 0.524
2.954ProLeu: 2.954 ± 0.487
0.933ProMet: 0.933 ± 0.269
0.855ProAsn: 0.855 ± 0.241
1.788ProPro: 1.788 ± 0.509
1.399ProGln: 1.399 ± 0.427
2.099ProArg: 2.099 ± 0.357
1.554ProSer: 1.554 ± 0.503
2.409ProThr: 2.409 ± 0.448
3.575ProVal: 3.575 ± 0.584
0.622ProTrp: 0.622 ± 0.21
2.021ProTyr: 2.021 ± 0.441
0.0ProXaa: 0.0 ± 0.0
Gln
6.762GlnAla: 6.762 ± 1.013
0.466GlnCys: 0.466 ± 0.176
1.166GlnAsp: 1.166 ± 0.266
2.332GlnGlu: 2.332 ± 0.532
1.554GlnPhe: 1.554 ± 0.307
3.731GlnGly: 3.731 ± 0.499
0.777GlnHis: 0.777 ± 0.262
1.865GlnIle: 1.865 ± 0.33
1.244GlnLys: 1.244 ± 0.305
4.275GlnLeu: 4.275 ± 0.597
1.943GlnMet: 1.943 ± 0.342
1.71GlnAsn: 1.71 ± 0.313
1.166GlnPro: 1.166 ± 0.277
2.72GlnGln: 2.72 ± 0.475
3.187GlnArg: 3.187 ± 0.511
1.71GlnSer: 1.71 ± 0.299
2.876GlnThr: 2.876 ± 0.541
3.187GlnVal: 3.187 ± 0.509
0.7GlnTrp: 0.7 ± 0.229
2.176GlnTyr: 2.176 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
5.829ArgAla: 5.829 ± 0.701
0.933ArgCys: 0.933 ± 0.34
4.042ArgAsp: 4.042 ± 0.416
3.575ArgGlu: 3.575 ± 0.715
2.332ArgPhe: 2.332 ± 0.446
3.964ArgGly: 3.964 ± 0.59
1.477ArgHis: 1.477 ± 0.338
2.798ArgIle: 2.798 ± 0.485
3.42ArgLys: 3.42 ± 0.564
4.974ArgLeu: 4.974 ± 0.587
1.865ArgMet: 1.865 ± 0.397
2.798ArgAsn: 2.798 ± 0.42
2.332ArgPro: 2.332 ± 0.525
2.798ArgGln: 2.798 ± 0.536
4.353ArgArg: 4.353 ± 0.56
4.275ArgSer: 4.275 ± 0.672
3.342ArgThr: 3.342 ± 0.552
4.586ArgVal: 4.586 ± 0.592
1.088ArgTrp: 1.088 ± 0.405
1.865ArgTyr: 1.865 ± 0.366
0.0ArgXaa: 0.0 ± 0.0
Ser
5.829SerAla: 5.829 ± 0.572
0.311SerCys: 0.311 ± 0.194
2.643SerAsp: 2.643 ± 0.465
3.498SerGlu: 3.498 ± 0.542
1.166SerPhe: 1.166 ± 0.306
5.363SerGly: 5.363 ± 0.661
1.01SerHis: 1.01 ± 0.238
2.021SerIle: 2.021 ± 0.46
2.72SerLys: 2.72 ± 0.453
5.985SerLeu: 5.985 ± 0.714
1.71SerMet: 1.71 ± 0.341
1.554SerAsn: 1.554 ± 0.357
1.943SerPro: 1.943 ± 0.331
1.788SerGln: 1.788 ± 0.38
3.109SerArg: 3.109 ± 0.435
2.332SerSer: 2.332 ± 0.382
3.031SerThr: 3.031 ± 0.442
3.342SerVal: 3.342 ± 0.494
0.855SerTrp: 0.855 ± 0.248
1.399SerTyr: 1.399 ± 0.427
0.0SerXaa: 0.0 ± 0.0
Thr
7.151ThrAla: 7.151 ± 0.735
1.088ThrCys: 1.088 ± 0.318
3.264ThrAsp: 3.264 ± 0.369
2.876ThrGlu: 2.876 ± 0.335
2.021ThrPhe: 2.021 ± 0.384
6.296ThrGly: 6.296 ± 0.994
1.01ThrHis: 1.01 ± 0.278
1.943ThrIle: 1.943 ± 0.324
2.798ThrLys: 2.798 ± 0.619
4.353ThrLeu: 4.353 ± 0.626
1.632ThrMet: 1.632 ± 0.28
1.632ThrAsn: 1.632 ± 0.236
4.508ThrPro: 4.508 ± 0.668
2.176ThrGln: 2.176 ± 0.405
3.264ThrArg: 3.264 ± 0.576
3.42ThrSer: 3.42 ± 0.596
2.643ThrThr: 2.643 ± 0.546
4.508ThrVal: 4.508 ± 0.557
0.7ThrTrp: 0.7 ± 0.175
2.021ThrTyr: 2.021 ± 0.455
0.0ThrXaa: 0.0 ± 0.0
Val
7.228ValAla: 7.228 ± 0.981
0.855ValCys: 0.855 ± 0.251
4.741ValAsp: 4.741 ± 0.592
3.731ValGlu: 3.731 ± 0.623
2.409ValPhe: 2.409 ± 0.418
5.596ValGly: 5.596 ± 0.908
1.088ValHis: 1.088 ± 0.345
2.643ValIle: 2.643 ± 0.537
2.72ValLys: 2.72 ± 0.575
5.596ValLeu: 5.596 ± 0.905
1.321ValMet: 1.321 ± 0.323
2.72ValAsn: 2.72 ± 0.387
4.275ValPro: 4.275 ± 0.643
3.731ValGln: 3.731 ± 0.655
4.819ValArg: 4.819 ± 0.729
3.964ValSer: 3.964 ± 0.501
3.808ValThr: 3.808 ± 0.814
4.43ValVal: 4.43 ± 0.809
0.544ValTrp: 0.544 ± 0.182
2.409ValTyr: 2.409 ± 0.49
0.0ValXaa: 0.0 ± 0.0
Trp
1.321TrpAla: 1.321 ± 0.242
0.389TrpCys: 0.389 ± 0.168
1.01TrpAsp: 1.01 ± 0.351
1.01TrpGlu: 1.01 ± 0.24
0.7TrpPhe: 0.7 ± 0.184
1.166TrpGly: 1.166 ± 0.269
0.311TrpHis: 0.311 ± 0.114
0.544TrpIle: 0.544 ± 0.211
0.622TrpLys: 0.622 ± 0.166
1.554TrpLeu: 1.554 ± 0.444
0.466TrpMet: 0.466 ± 0.199
0.544TrpAsn: 0.544 ± 0.212
0.389TrpPro: 0.389 ± 0.2
0.622TrpGln: 0.622 ± 0.197
0.466TrpArg: 0.466 ± 0.194
0.7TrpSer: 0.7 ± 0.252
0.622TrpThr: 0.622 ± 0.231
0.7TrpVal: 0.7 ± 0.359
0.078TrpTrp: 0.078 ± 0.076
0.544TrpTyr: 0.544 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.021TyrAla: 2.021 ± 0.382
0.544TyrCys: 0.544 ± 0.216
1.71TyrAsp: 1.71 ± 0.447
1.788TyrGlu: 1.788 ± 0.375
1.321TyrPhe: 1.321 ± 0.436
2.332TyrGly: 2.332 ± 0.411
0.7TyrHis: 0.7 ± 0.205
1.865TyrIle: 1.865 ± 0.527
1.321TyrLys: 1.321 ± 0.408
2.409TyrLeu: 2.409 ± 0.465
0.777TyrMet: 0.777 ± 0.241
1.166TyrAsn: 1.166 ± 0.31
1.554TyrPro: 1.554 ± 0.305
1.71TyrGln: 1.71 ± 0.493
2.798TyrArg: 2.798 ± 0.562
1.788TyrSer: 1.788 ± 0.474
2.254TyrThr: 2.254 ± 0.459
2.176TyrVal: 2.176 ± 0.447
0.622TyrTrp: 0.622 ± 0.243
0.544TyrTyr: 0.544 ± 0.193
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12867 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski