Capybara microvirus Cap1_SP_83
Average proteome isoelectric point is 5.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7M1|A0A4P8W7M1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_83 OX=2584794 PE=4 SV=1
MM1 pKa = 7.57 SLLDD5 pKa = 4.44 NIKK8 pKa = 9.66 TSAKK12 pKa = 9.64 EE13 pKa = 3.75 DD14 pKa = 3.69 SLLVSQSNSALLDD27 pKa = 3.74 SLAHH31 pKa = 5.95 QFDD34 pKa = 3.95 GTDD37 pKa = 3.6 FEE39 pKa = 6.36 SRR41 pKa = 11.84 AQSNPYY47 pKa = 8.2 LTSSYY52 pKa = 10.96 GSKK55 pKa = 9.06 TLWDD59 pKa = 4.55 KK60 pKa = 11.28 IGDD63 pKa = 4.05 LIGFRR68 pKa = 11.84 TSEE71 pKa = 4.12 DD72 pKa = 3.47 VYY74 pKa = 10.64 EE75 pKa = 4.19 TDD77 pKa = 5.0 RR78 pKa = 11.84 IQAAKK83 pKa = 10.28 DD84 pKa = 3.37 YY85 pKa = 10.6 NAQLQQLYY93 pKa = 10.76 DD94 pKa = 3.69 EE95 pKa = 4.6 QAYY98 pKa = 10.57 NSPSAQASRR107 pKa = 11.84 YY108 pKa = 9.11 RR109 pKa = 11.84 EE110 pKa = 3.79 AGINPDD116 pKa = 2.99 INGSVSSGEE125 pKa = 3.68 ASEE128 pKa = 5.24 FSQEE132 pKa = 3.77 QTSPVSPPASYY143 pKa = 11.49 NFGQFSKK150 pKa = 10.98 VCFDD154 pKa = 5.46 AIQVALNLADD164 pKa = 3.98 GFTSLRR170 pKa = 11.84 GKK172 pKa = 10.7 FIDD175 pKa = 3.53 QAGAEE180 pKa = 4.19 LSQYY184 pKa = 10.88 SAVDD188 pKa = 3.61 SVALLDD194 pKa = 4.66 FLSQEE199 pKa = 4.38 QISDD203 pKa = 3.75 SDD205 pKa = 3.62 FDD207 pKa = 5.45 DD208 pKa = 4.56 YY209 pKa = 11.87 PNMIAPAVKK218 pKa = 10.3 VEE220 pKa = 3.99 KK221 pKa = 11.0 GPDD224 pKa = 3.13 KK225 pKa = 10.79 FSSKK229 pKa = 10.04 RR230 pKa = 11.84 LQSAYY235 pKa = 10.25 DD236 pKa = 3.37 RR237 pKa = 11.84 KK238 pKa = 10.39 LSQLIRR244 pKa = 11.84 SGSLRR249 pKa = 11.84 GQGIRR254 pKa = 11.84 MQGLNQYY261 pKa = 10.08 YY262 pKa = 10.32 GDD264 pKa = 3.85 RR265 pKa = 11.84 LSMFEE270 pKa = 4.23 SKK272 pKa = 10.32 NAYY275 pKa = 9.73 GLDD278 pKa = 3.86 GLDD281 pKa = 3.47 SSLDD285 pKa = 3.24 IITRR289 pKa = 11.84 HH290 pKa = 5.93 LGSLRR295 pKa = 11.84 KK296 pKa = 8.67 ATEE299 pKa = 3.66 EE300 pKa = 3.97 ATLKK304 pKa = 11.0 ASAAQGKK311 pKa = 9.48 FDD313 pKa = 3.58 EE314 pKa = 6.0 AYY316 pKa = 8.87 TQGLDD321 pKa = 3.36 PSQVSEE327 pKa = 4.78 SDD329 pKa = 3.14 MASYY333 pKa = 10.64 KK334 pKa = 10.62 SSEE337 pKa = 3.96 QEE339 pKa = 3.95 KK340 pKa = 10.45 EE341 pKa = 3.54 ISEE344 pKa = 4.28 ALNKK348 pKa = 8.46 TVNGIVSDD356 pKa = 3.75 LRR358 pKa = 11.84 KK359 pKa = 10.6 NIDD362 pKa = 4.58 DD363 pKa = 4.32 DD364 pKa = 4.13 PKK366 pKa = 10.42 HH367 pKa = 6.55 AFFSRR372 pKa = 11.84 LLLIGFYY379 pKa = 10.86 LLSNNLMPSLSFGRR393 pKa = 11.84 SSSQGAYY400 pKa = 7.92 TGKK403 pKa = 10.17 FGSSARR409 pKa = 11.84 SSSGFNFGIKK419 pKa = 9.79
Molecular weight: 45.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.766
IPC2_protein 4.762
IPC_protein 4.711
Toseland 4.546
ProMoST 4.838
Dawson 4.673
Bjellqvist 4.825
Wikipedia 4.571
Rodwell 4.546
Grimsley 4.457
Solomon 4.673
Lehninger 4.635
Nozaki 4.787
DTASelect 5.003
Thurlkill 4.558
EMBOSS 4.584
Sillero 4.825
Patrickios 4.037
IPC_peptide 4.673
IPC2_peptide 4.813
IPC2.peptide.svr19 4.833
Protein with the highest isoelectric point:
>tr|A0A4P8W7L4|A0A4P8W7L4_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_83 OX=2584794 PE=4 SV=1
MM1 pKa = 7.65 CSSPITIRR9 pKa = 11.84 NPNYY13 pKa = 10.3 AFSRR17 pKa = 11.84 DD18 pKa = 3.21 STGNIKK24 pKa = 10.09 PLIFHH29 pKa = 7.51 DD30 pKa = 4.01 SNIRR34 pKa = 11.84 HH35 pKa = 5.74 VADD38 pKa = 3.68 SSPYY42 pKa = 7.76 MQVPCGHH49 pKa = 6.75 CPDD52 pKa = 3.7 CVARR56 pKa = 11.84 KK57 pKa = 10.05 SSDD60 pKa = 3.01 LAQRR64 pKa = 11.84 ASIEE68 pKa = 4.13 SLYY71 pKa = 11.1 SHH73 pKa = 6.73 VFFLTLTYY81 pKa = 11.28 NNDD84 pKa = 3.65 SLPHH88 pKa = 6.24 FLTSEE93 pKa = 4.03 GGNIPFADD101 pKa = 4.42 FSDD104 pKa = 3.78 LQKK107 pKa = 10.21 MFKK110 pKa = 10.53 LIRR113 pKa = 11.84 KK114 pKa = 8.33 YY115 pKa = 11.14 NEE117 pKa = 3.21 ILRR120 pKa = 11.84 PFKK123 pKa = 10.51 YY124 pKa = 10.33 VSVSEE129 pKa = 4.44 RR130 pKa = 11.84 GTEE133 pKa = 4.0 GARR136 pKa = 11.84 PHH138 pKa = 5.73 FHH140 pKa = 6.29 VLLFVEE146 pKa = 5.23 KK147 pKa = 10.63 LPKK150 pKa = 9.97 DD151 pKa = 4.02 DD152 pKa = 5.36 YY153 pKa = 12.05 NDD155 pKa = 3.49 ILNLEE160 pKa = 4.77 SYY162 pKa = 10.62 LSSLFLRR169 pKa = 11.84 RR170 pKa = 11.84 WQRR173 pKa = 11.84 NYY175 pKa = 10.93 GSTRR179 pKa = 11.84 NPDD182 pKa = 3.31 YY183 pKa = 11.28 RR184 pKa = 11.84 NLCTFVDD191 pKa = 3.58 KK192 pKa = 10.75 STLFRR197 pKa = 11.84 KK198 pKa = 7.64 QTNFDD203 pKa = 3.33 LHH205 pKa = 5.76 YY206 pKa = 10.86 VKK208 pKa = 10.54 PDD210 pKa = 3.32 VNNGISSVVYY220 pKa = 9.58 YY221 pKa = 10.42 CMKK224 pKa = 10.85 YY225 pKa = 8.33 MLKK228 pKa = 10.37 EE229 pKa = 3.99 SSHH232 pKa = 6.7 DD233 pKa = 3.99 SKK235 pKa = 11.43 LHH237 pKa = 5.26 YY238 pKa = 10.03 ALKK241 pKa = 10.67 SSLPPGEE248 pKa = 4.94 AAQSWSVVRR257 pKa = 11.84 SKK259 pKa = 10.62 RR260 pKa = 11.84 ACSLDD265 pKa = 3.39 FGRR268 pKa = 11.84 KK269 pKa = 8.89 SDD271 pKa = 3.65 PSVLKK276 pKa = 10.51 RR277 pKa = 11.84 IHH279 pKa = 7.54 DD280 pKa = 4.31 DD281 pKa = 3.25 VDD283 pKa = 3.04 KK284 pKa = 11.21 CRR286 pKa = 11.84 YY287 pKa = 9.46 LYY289 pKa = 7.42 NTPVFCYY296 pKa = 9.59 PDD298 pKa = 3.79 CALTAPLSRR307 pKa = 11.84 YY308 pKa = 10.11 YY309 pKa = 11.07 YY310 pKa = 10.46 DD311 pKa = 5.26 ALSLDD316 pKa = 3.32 QVQYY320 pKa = 11.7 YY321 pKa = 10.42 NDD323 pKa = 4.97 LYY325 pKa = 11.27 QSLPPSSSLDD335 pKa = 3.38 YY336 pKa = 10.89 DD337 pKa = 3.61 SRR339 pKa = 11.84 LLKK342 pKa = 10.92 DD343 pKa = 2.9 SDD345 pKa = 3.17 KK346 pKa = 10.71 FRR348 pKa = 11.84 RR349 pKa = 11.84 LKK351 pKa = 10.16 TDD353 pKa = 3.13 VFSEE357 pKa = 4.06 NVNIYY362 pKa = 10.89 EE363 pKa = 4.68 NILL366 pKa = 3.49
Molecular weight: 42.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.402
IPC2_protein 7.629
IPC_protein 7.483
Toseland 7.132
ProMoST 8.024
Dawson 8.156
Bjellqvist 8.521
Wikipedia 8.083
Rodwell 8.17
Grimsley 7.176
Solomon 8.229
Lehninger 8.244
Nozaki 8.639
DTASelect 8.258
Thurlkill 8.302
EMBOSS 8.361
Sillero 8.624
Patrickios 3.745
IPC_peptide 8.214
IPC2_peptide 7.585
IPC2.peptide.svr19 7.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1745
73
654
290.8
32.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.986 ± 1.052
1.605 ± 0.463
7.679 ± 0.365
3.381 ± 0.469
6.991 ± 0.909
4.814 ± 0.854
2.006 ± 0.416
4.928 ± 0.571
4.527 ± 0.677
10.372 ± 1.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.777 ± 0.214
5.272 ± 0.601
4.069 ± 0.572
3.61 ± 0.969
5.1 ± 0.429
13.123 ± 0.749
3.725 ± 0.409
5.903 ± 0.889
0.573 ± 0.151
5.559 ± 0.773
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here