Salanga virus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5XLH5|U5XLH5_9VIRU Replicase OS=Salanga virus OX=1416745 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 5.81 RR3 pKa = 11.84 YY4 pKa = 10.07 LHH6 pKa = 5.99 NRR8 pKa = 11.84 AIVHH12 pKa = 6.5 RR13 pKa = 11.84 LHH15 pKa = 6.91 GSSNLPVVTFKK26 pKa = 11.2 VIEE29 pKa = 4.31 DD30 pKa = 5.08 DD31 pKa = 3.34 IDD33 pKa = 3.44 EE34 pKa = 4.64 HH35 pKa = 7.76 VAVHH39 pKa = 5.13 QCVEE43 pKa = 4.5 VVLHH47 pKa = 4.95 SHH49 pKa = 6.48 RR50 pKa = 11.84 LSRR53 pKa = 11.84 AEE55 pKa = 3.81 GHH57 pKa = 4.86 SLKK60 pKa = 10.43 WFLEE64 pKa = 4.02 RR65 pKa = 11.84 GLFPARR71 pKa = 11.84 SGDD74 pKa = 4.47 LIWSSCITRR83 pKa = 11.84 IEE85 pKa = 4.13 PQDD88 pKa = 3.91 LNSYY92 pKa = 10.38 LYY94 pKa = 11.03 GFAALPRR101 pKa = 11.84 EE102 pKa = 4.49 DD103 pKa = 5.11 FNCLQLRR110 pKa = 11.84 NVRR113 pKa = 11.84 KK114 pKa = 9.79 ALEE117 pKa = 4.2 WPTGQLSFEE126 pKa = 4.23 FFDD129 pKa = 5.08 LEE131 pKa = 4.56 FPGKK135 pKa = 9.62 YY136 pKa = 8.13 VWAFEE141 pKa = 3.88 NMRR144 pKa = 11.84 RR145 pKa = 11.84 TIQLLSMMSSSDD157 pKa = 3.33 QVEE160 pKa = 5.18 DD161 pKa = 4.81 IICDD165 pKa = 3.37 AYY167 pKa = 11.23 DD168 pKa = 3.48 CVLRR172 pKa = 11.84 AGSDD176 pKa = 2.99 LGLAHH181 pKa = 6.43 STFPGNNIVYY191 pKa = 7.03 EE192 pKa = 4.02 VCYY195 pKa = 10.31 IQLLKK200 pKa = 10.69 AVQAYY205 pKa = 7.92 STDD208 pKa = 3.22 IQQVSGFPDD217 pKa = 3.14 NAVTIFLKK225 pKa = 10.79 DD226 pKa = 2.98 IMPYY230 pKa = 10.08 VRR232 pKa = 11.84 DD233 pKa = 3.54 RR234 pKa = 11.84 FPGVRR239 pKa = 11.84 EE240 pKa = 3.95 PMSNPLPEE248 pKa = 4.84 PSEE251 pKa = 4.02 HH252 pKa = 6.39 QLNVWALPDD261 pKa = 3.48 EE262 pKa = 5.12 GIEE265 pKa = 4.04 EE266 pKa = 5.41 DD267 pKa = 3.87 IDD269 pKa = 3.56
Molecular weight: 30.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.806
IPC2_protein 4.876
IPC_protein 4.825
Toseland 4.749
ProMoST 4.902
Dawson 4.813
Bjellqvist 4.965
Wikipedia 4.698
Rodwell 4.724
Grimsley 4.66
Solomon 4.813
Lehninger 4.774
Nozaki 4.94
DTASelect 5.105
Thurlkill 4.749
EMBOSS 4.736
Sillero 5.003
Patrickios 3.668
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 4.949
Protein with the highest isoelectric point:
>tr|U5XJM5|U5XJM5_9VIRU Nonstructural protein OS=Salanga virus OX=1416745 PE=4 SV=1
MM1 pKa = 8.27 DD2 pKa = 4.04 YY3 pKa = 11.53 AKK5 pKa = 10.37 IALDD9 pKa = 3.65 FAGEE13 pKa = 4.53 GISQVEE19 pKa = 3.82 IAKK22 pKa = 9.16 WVEE25 pKa = 3.56 DD26 pKa = 3.74 FAYY29 pKa = 10.39 QGFDD33 pKa = 2.8 AKK35 pKa = 10.47 RR36 pKa = 11.84 VVEE39 pKa = 4.1 LVQTRR44 pKa = 11.84 GSGRR48 pKa = 11.84 NWKK51 pKa = 9.73 QDD53 pKa = 3.35 VAKK56 pKa = 9.71 MIVIALTRR64 pKa = 11.84 GNKK67 pKa = 7.49 IAKK70 pKa = 7.94 MKK72 pKa = 10.82 QKK74 pKa = 10.2 MSDD77 pKa = 3.12 KK78 pKa = 10.45 GKK80 pKa = 10.57 EE81 pKa = 4.0 SLTEE85 pKa = 3.82 LEE87 pKa = 3.94 RR88 pKa = 11.84 VYY90 pKa = 10.13 MLKK93 pKa = 10.38 SGNPGRR99 pKa = 11.84 DD100 pKa = 3.4 DD101 pKa = 3.55 LTLSRR106 pKa = 11.84 IAVAFAPWTVGALNHH121 pKa = 6.99 LDD123 pKa = 2.83 GHH125 pKa = 6.53 LPVNGKK131 pKa = 10.19 DD132 pKa = 3.3 MDD134 pKa = 3.93 EE135 pKa = 4.2 HH136 pKa = 5.37 VQRR139 pKa = 11.84 YY140 pKa = 6.1 PRR142 pKa = 11.84 CMMHH146 pKa = 6.86 PAFGSLIDD154 pKa = 3.87 RR155 pKa = 11.84 NLPEE159 pKa = 4.12 VTVSQLINAHH169 pKa = 6.72 CIFLDD174 pKa = 3.26 KK175 pKa = 10.05 FARR178 pKa = 11.84 VINPNLRR185 pKa = 11.84 GKK187 pKa = 8.37 TKK189 pKa = 10.52 QEE191 pKa = 3.73 VAKK194 pKa = 10.7 SYY196 pKa = 7.4 EE197 pKa = 4.18 QPLKK201 pKa = 10.79 AAVSSTFFSLEE212 pKa = 3.68 QKK214 pKa = 10.61 RR215 pKa = 11.84 GVLKK219 pKa = 10.82 NLGLIDD225 pKa = 4.25 EE226 pKa = 4.51 NLKK229 pKa = 8.52 VTEE232 pKa = 4.23 VVKK235 pKa = 10.47 RR236 pKa = 11.84 ASDD239 pKa = 3.75 FWTQAFF245 pKa = 3.82
Molecular weight: 27.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.858
IPC2_protein 8.595
IPC_protein 8.521
Toseland 9.458
ProMoST 9.077
Dawson 9.648
Bjellqvist 9.311
Wikipedia 9.765
Rodwell 10.116
Grimsley 9.692
Solomon 9.706
Lehninger 9.692
Nozaki 9.531
DTASelect 9.282
Thurlkill 9.516
EMBOSS 9.867
Sillero 9.589
Patrickios 7.658
IPC_peptide 9.706
IPC2_peptide 7.79
IPC2.peptide.svr19 7.851
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
4035
245
2123
1008.8
114.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.527 ± 0.414
2.677 ± 0.741
6.245 ± 0.351
6.295 ± 0.327
4.411 ± 0.133
5.651 ± 0.252
2.354 ± 0.151
6.072 ± 0.369
6.221 ± 0.367
9.021 ± 0.406
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.47 ± 0.572
4.04 ± 0.27
3.866 ± 0.175
3.371 ± 0.274
5.75 ± 0.167
8.848 ± 0.69
5.353 ± 0.461
6.667 ± 0.391
1.388 ± 0.115
2.776 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here