Amino acid dipepetide frequency for Babesia sp. Xinjiang

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.461AlaAla: 5.461 ± 0.083	1.185AlaCys: 1.185 ± 0.028	3.558AlaAsp: 3.558 ± 0.047	4.151AlaGlu: 4.151 ± 0.058	2.617AlaPhe: 2.617 ± 0.047	3.287AlaGly: 3.287 ± 0.043	1.538AlaHis: 1.538 ± 0.031	4.083AlaIle: 4.083 ± 0.066	3.946AlaLys: 3.946 ± 0.064	7.064AlaLeu: 7.064 ± 0.08	1.793AlaMet: 1.793 ± 0.035	2.78AlaAsn: 2.78 ± 0.039	2.711AlaPro: 2.711 ± 0.045	2.229AlaGln: 2.229 ± 0.037	3.44AlaArg: 3.44 ± 0.049	5.266AlaSer: 5.266 ± 0.072	4.337AlaThr: 4.337 ± 0.062	4.826AlaVal: 4.826 ± 0.067	0.524AlaTrp: 0.524 ± 0.018	2.051AlaTyr: 2.051 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.402CysAla: 1.402 ± 0.028	0.609CysCys: 0.609 ± 0.026	1.386CysAsp: 1.386 ± 0.031	1.15CysGlu: 1.15 ± 0.029	0.87CysPhe: 0.87 ± 0.022	1.41CysGly: 1.41 ± 0.032	0.624CysHis: 0.624 ± 0.02	1.278CysIle: 1.278 ± 0.034	1.236CysLys: 1.236 ± 0.031	2.061CysLeu: 2.061 ± 0.044	0.478CysMet: 0.478 ± 0.017	1.046CysAsn: 1.046 ± 0.029	0.837CysPro: 0.837 ± 0.027	0.637CysGln: 0.637 ± 0.019	1.214CysArg: 1.214 ± 0.031	1.63CysSer: 1.63 ± 0.036	1.124CysThr: 1.124 ± 0.032	1.543CysVal: 1.543 ± 0.034	0.202CysTrp: 0.202 ± 0.011	0.743CysTyr: 0.743 ± 0.02	0.001CysXaa: 0.001 ± 0.001
Asp	4.668AspAla: 4.668 ± 0.06	1.133AspCys: 1.133 ± 0.029	4.506AspAsp: 4.506 ± 0.066	4.387AspGlu: 4.387 ± 0.067	2.287AspPhe: 2.287 ± 0.045	3.694AspGly: 3.694 ± 0.053	1.357AspHis: 1.357 ± 0.028	4.077AspIle: 4.077 ± 0.054	2.779AspLys: 2.779 ± 0.047	5.128AspLeu: 5.128 ± 0.063	1.544AspMet: 1.544 ± 0.028	2.555AspAsn: 2.555 ± 0.038	2.487AspPro: 2.487 ± 0.046	1.429AspGln: 1.429 ± 0.032	2.883AspArg: 2.883 ± 0.041	4.571AspSer: 4.571 ± 0.073	3.628AspThr: 3.628 ± 0.055	4.873AspVal: 4.873 ± 0.057	0.546AspTrp: 0.546 ± 0.016	1.973AspTyr: 1.973 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	4.324GluAla: 4.324 ± 0.069	1.284GluCys: 1.284 ± 0.024	3.767GluAsp: 3.767 ± 0.054	4.914GluGlu: 4.914 ± 0.088	2.328GluPhe: 2.328 ± 0.037	3.21GluGly: 3.21 ± 0.047	1.677GluHis: 1.677 ± 0.035	3.384GluIle: 3.384 ± 0.053	3.468GluLys: 3.468 ± 0.054	6.276GluLeu: 6.276 ± 0.07	1.645GluMet: 1.645 ± 0.034	2.856GluAsn: 2.856 ± 0.046	2.634GluPro: 2.634 ± 0.044	2.36GluGln: 2.36 ± 0.048	3.958GluArg: 3.958 ± 0.061	4.892GluSer: 4.892 ± 0.066	3.447GluThr: 3.447 ± 0.044	3.975GluVal: 3.975 ± 0.057	0.624GluTrp: 0.624 ± 0.019	2.086GluTyr: 2.086 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	2.575PheAla: 2.575 ± 0.045	0.916PheCys: 0.916 ± 0.025	2.587PheAsp: 2.587 ± 0.043	2.356PheGlu: 2.356 ± 0.044	1.473PhePhe: 1.473 ± 0.034	2.485PheGly: 2.485 ± 0.054	0.982PheHis: 0.982 ± 0.025	2.097PheIle: 2.097 ± 0.037	2.261PheLys: 2.261 ± 0.04	3.547PheLeu: 3.547 ± 0.048	0.984PheMet: 0.984 ± 0.028	1.907PheAsn: 1.907 ± 0.036	1.334PhePro: 1.334 ± 0.031	1.207PheGln: 1.207 ± 0.025	2.104PheArg: 2.104 ± 0.036	2.839PheSer: 2.839 ± 0.047	2.202PheThr: 2.202 ± 0.037	2.972PheVal: 2.972 ± 0.047	0.463PheTrp: 0.463 ± 0.015	1.394PheTyr: 1.394 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	3.352GlyAla: 3.352 ± 0.045	1.256GlyCys: 1.256 ± 0.03	3.664GlyAsp: 3.664 ± 0.058	3.232GlyGlu: 3.232 ± 0.047	2.303GlyPhe: 2.303 ± 0.042	3.558GlyGly: 3.558 ± 0.062	1.454GlyHis: 1.454 ± 0.032	3.481GlyIle: 3.481 ± 0.049	3.312GlyLys: 3.312 ± 0.051	4.524GlyLeu: 4.524 ± 0.055	1.377GlyMet: 1.377 ± 0.032	2.745GlyAsn: 2.745 ± 0.053	1.871GlyPro: 1.871 ± 0.04	1.591GlyGln: 1.591 ± 0.032	3.223GlyArg: 3.223 ± 0.049	4.798GlySer: 4.798 ± 0.069	3.606GlyThr: 3.606 ± 0.065	3.833GlyVal: 3.833 ± 0.05	0.555GlyTrp: 0.555 ± 0.02	2.097GlyTyr: 2.097 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	1.494HisAla: 1.494 ± 0.03	0.596HisCys: 0.596 ± 0.02	1.387HisAsp: 1.387 ± 0.029	1.432HisGlu: 1.432 ± 0.029	1.091HisPhe: 1.091 ± 0.025	1.56HisGly: 1.56 ± 0.033	0.725HisHis: 0.725 ± 0.023	1.71HisIle: 1.71 ± 0.029	1.342HisLys: 1.342 ± 0.028	2.7HisLeu: 2.7 ± 0.039	0.742HisMet: 0.742 ± 0.022	1.115HisAsn: 1.115 ± 0.027	1.193HisPro: 1.193 ± 0.026	0.823HisGln: 0.823 ± 0.024	1.651HisArg: 1.651 ± 0.035	1.922HisSer: 1.922 ± 0.035	1.352HisThr: 1.352 ± 0.032	2.009HisVal: 2.009 ± 0.037	0.287HisTrp: 0.287 ± 0.014	0.909HisTyr: 0.909 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	4.029IleAla: 4.029 ± 0.05	1.431IleCys: 1.431 ± 0.031	3.543IleAsp: 3.543 ± 0.045	3.372IleGlu: 3.372 ± 0.05	2.231IlePhe: 2.231 ± 0.04	3.061IleGly: 3.061 ± 0.051	1.379IleHis: 1.379 ± 0.031	2.826IleIle: 2.826 ± 0.05	3.174IleLys: 3.174 ± 0.051	5.092IleLeu: 5.092 ± 0.059	1.372IleMet: 1.372 ± 0.027	2.492IleAsn: 2.492 ± 0.038	2.65IlePro: 2.65 ± 0.039	1.896IleGln: 1.896 ± 0.037	3.212IleArg: 3.212 ± 0.042	4.544IleSer: 4.544 ± 0.059	3.213IleThr: 3.213 ± 0.047	3.775IleVal: 3.775 ± 0.051	0.541IleTrp: 0.541 ± 0.021	2.022IleTyr: 2.022 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	3.601LysAla: 3.601 ± 0.046	1.206LysCys: 1.206 ± 0.031	3.122LysAsp: 3.122 ± 0.05	3.706LysGlu: 3.706 ± 0.056	1.892LysPhe: 1.892 ± 0.034	3.079LysGly: 3.079 ± 0.05	1.631LysHis: 1.631 ± 0.034	2.974LysIle: 2.974 ± 0.045	3.44LysLys: 3.44 ± 0.065	5.653LysLeu: 5.653 ± 0.076	1.425LysMet: 1.425 ± 0.03	2.552LysAsn: 2.552 ± 0.049	2.417LysPro: 2.417 ± 0.047	2.33LysGln: 2.33 ± 0.048	4.116LysArg: 4.116 ± 0.055	4.118LysSer: 4.118 ± 0.055	3.155LysThr: 3.155 ± 0.049	3.76LysVal: 3.76 ± 0.048	0.601LysTrp: 0.601 ± 0.02	1.952LysTyr: 1.952 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	6.0LeuAla: 6.0 ± 0.059	2.353LeuCys: 2.353 ± 0.047	5.594LeuAsp: 5.594 ± 0.073	6.161LeuGlu: 6.161 ± 0.076	3.825LeuPhe: 3.825 ± 0.057	4.984LeuGly: 4.984 ± 0.061	2.618LeuHis: 2.618 ± 0.042	4.546LeuIle: 4.546 ± 0.053	5.861LeuLys: 5.861 ± 0.065	10.217LeuLeu: 10.217 ± 0.109	2.388LeuMet: 2.388 ± 0.042	4.117LeuAsn: 4.117 ± 0.054	4.344LeuPro: 4.344 ± 0.053	3.945LeuGln: 3.945 ± 0.056	6.466LeuArg: 6.466 ± 0.082	7.915LeuSer: 7.915 ± 0.085	4.926LeuThr: 4.926 ± 0.059	5.828LeuVal: 5.828 ± 0.059	1.007LeuTrp: 1.007 ± 0.028	3.507LeuTyr: 3.507 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.919MetAla: 1.919 ± 0.039	0.503MetCys: 0.503 ± 0.015	1.605MetAsp: 1.605 ± 0.027	1.696MetGlu: 1.696 ± 0.033	0.9MetPhe: 0.9 ± 0.023	1.401MetGly: 1.401 ± 0.03	0.64MetHis: 0.64 ± 0.018	1.168MetIle: 1.168 ± 0.026	1.447MetLys: 1.447 ± 0.032	2.739MetLeu: 2.739 ± 0.042	0.689MetMet: 0.689 ± 0.023	0.993MetAsn: 0.993 ± 0.026	1.237MetPro: 1.237 ± 0.033	1.062MetGln: 1.062 ± 0.03	1.561MetArg: 1.561 ± 0.032	1.862MetSer: 1.862 ± 0.037	1.354MetThr: 1.354 ± 0.03	1.607MetVal: 1.607 ± 0.03	0.247MetTrp: 0.247 ± 0.012	0.817MetTyr: 0.817 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.269AsnAla: 3.269 ± 0.048	0.881AsnCys: 0.881 ± 0.026	2.736AsnAsp: 2.736 ± 0.042	2.786AsnGlu: 2.786 ± 0.043	1.704AsnPhe: 1.704 ± 0.035	2.817AsnGly: 2.817 ± 0.048	1.035AsnHis: 1.035 ± 0.027	2.9AsnIle: 2.9 ± 0.048	2.448AsnLys: 2.448 ± 0.042	3.845AsnLeu: 3.845 ± 0.051	1.263AsnMet: 1.263 ± 0.03	2.088AsnAsn: 2.088 ± 0.037	1.922AsnPro: 1.922 ± 0.036	1.315AsnGln: 1.315 ± 0.029	2.485AsnArg: 2.485 ± 0.041	3.179AsnSer: 3.179 ± 0.045	2.714AsnThr: 2.714 ± 0.046	3.634AsnVal: 3.634 ± 0.05	0.434AsnTrp: 0.434 ± 0.016	1.473AsnTyr: 1.473 ± 0.032	0.001AsnXaa: 0.001 ± 0.001
Pro	2.547ProAla: 2.547 ± 0.048	0.749ProCys: 0.749 ± 0.023	2.27ProAsp: 2.27 ± 0.036	2.946ProGlu: 2.946 ± 0.048	1.748ProPhe: 1.748 ± 0.035	2.26ProGly: 2.26 ± 0.044	1.008ProHis: 1.008 ± 0.023	2.28ProIle: 2.28 ± 0.04	2.494ProLys: 2.494 ± 0.05	4.081ProLeu: 4.081 ± 0.047	1.045ProMet: 1.045 ± 0.029	1.972ProAsn: 1.972 ± 0.036	2.555ProPro: 2.555 ± 0.065	1.703ProGln: 1.703 ± 0.036	2.378ProArg: 2.378 ± 0.043	3.757ProSer: 3.757 ± 0.054	2.543ProThr: 2.543 ± 0.042	3.02ProVal: 3.02 ± 0.05	0.447ProTrp: 0.447 ± 0.016	1.38ProTyr: 1.38 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.911GlnAla: 1.911 ± 0.035	0.848GlnCys: 0.848 ± 0.029	1.65GlnAsp: 1.65 ± 0.03	2.12GlnGlu: 2.12 ± 0.041	1.278GlnPhe: 1.278 ± 0.027	1.686GlnGly: 1.686 ± 0.032	1.15GlnHis: 1.15 ± 0.029	1.795GlnIle: 1.795 ± 0.035	2.011GlnLys: 2.011 ± 0.045	4.02GlnLeu: 4.02 ± 0.051	1.06GlnMet: 1.06 ± 0.028	1.609GlnAsn: 1.609 ± 0.034	1.574GlnPro: 1.574 ± 0.033	1.878GlnGln: 1.878 ± 0.055	2.564GlnArg: 2.564 ± 0.048	2.628GlnSer: 2.628 ± 0.047	1.796GlnThr: 1.796 ± 0.034	2.027GlnVal: 2.027 ± 0.036	0.39GlnTrp: 0.39 ± 0.014	1.239GlnTyr: 1.239 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	3.432ArgAla: 3.432 ± 0.049	1.423ArgCys: 1.423 ± 0.034	3.213ArgAsp: 3.213 ± 0.05	3.647ArgGlu: 3.647 ± 0.055	2.41ArgPhe: 2.41 ± 0.042	3.173ArgGly: 3.173 ± 0.046	1.829ArgHis: 1.829 ± 0.038	3.606ArgIle: 3.606 ± 0.05	3.487ArgLys: 3.487 ± 0.064	5.932ArgLeu: 5.932 ± 0.073	1.635ArgMet: 1.635 ± 0.031	2.869ArgAsn: 2.869 ± 0.042	2.151ArgPro: 2.151 ± 0.042	2.328ArgGln: 2.328 ± 0.039	4.754ArgArg: 4.754 ± 0.076	4.577ArgSer: 4.577 ± 0.062	2.919ArgThr: 2.919 ± 0.045	3.751ArgVal: 3.751 ± 0.051	0.654ArgTrp: 0.654 ± 0.019	2.254ArgTyr: 2.254 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	5.243SerAla: 5.243 ± 0.062	1.591SerCys: 1.591 ± 0.036	5.227SerAsp: 5.227 ± 0.074	4.706SerGlu: 4.706 ± 0.063	2.963SerPhe: 2.963 ± 0.054	4.875SerGly: 4.875 ± 0.059	2.13SerHis: 2.13 ± 0.038	4.237SerIle: 4.237 ± 0.071	4.371SerLys: 4.371 ± 0.059	7.146SerLeu: 7.146 ± 0.081	1.865SerMet: 1.865 ± 0.036	3.5SerAsn: 3.5 ± 0.049	3.262SerPro: 3.262 ± 0.052	2.823SerGln: 2.823 ± 0.041	4.607SerArg: 4.607 ± 0.064	7.38SerSer: 7.38 ± 0.105	4.824SerThr: 4.824 ± 0.071	5.391SerVal: 5.391 ± 0.07	0.743SerTrp: 0.743 ± 0.021	2.389SerTyr: 2.389 ± 0.036	0.001SerXaa: 0.001 ± 0.001
Thr	4.04ThrAla: 4.04 ± 0.056	1.119ThrCys: 1.119 ± 0.031	3.274ThrAsp: 3.274 ± 0.045	3.236ThrGlu: 3.236 ± 0.05	2.366ThrPhe: 2.366 ± 0.039	3.302ThrGly: 3.302 ± 0.061	1.459ThrHis: 1.459 ± 0.031	3.112ThrIle: 3.112 ± 0.047	3.181ThrLys: 3.181 ± 0.055	5.765ThrLeu: 5.765 ± 0.064	1.313ThrMet: 1.313 ± 0.029	2.528ThrAsn: 2.528 ± 0.048	3.04ThrPro: 3.04 ± 0.05	2.019ThrGln: 2.019 ± 0.041	2.933ThrArg: 2.933 ± 0.044	4.648ThrSer: 4.648 ± 0.06	3.75ThrThr: 3.75 ± 0.079	4.174ThrVal: 4.174 ± 0.039	0.524ThrTrp: 0.524 ± 0.018	1.696ThrTyr: 1.696 ± 0.035	0.001ThrXaa: 0.001 ± 0.001
Val	4.946ValAla: 4.946 ± 0.064	1.441ValCys: 1.441 ± 0.034	4.643ValAsp: 4.643 ± 0.051	4.708ValGlu: 4.708 ± 0.059	2.575ValPhe: 2.575 ± 0.043	3.539ValGly: 3.539 ± 0.05	1.613ValHis: 1.613 ± 0.032	3.683ValIle: 3.683 ± 0.049	3.986ValLys: 3.986 ± 0.054	6.523ValLeu: 6.523 ± 0.076	1.61ValMet: 1.61 ± 0.035	2.964ValAsn: 2.964 ± 0.04	3.32ValPro: 3.32 ± 0.051	2.211ValGln: 2.211 ± 0.042	3.568ValArg: 3.568 ± 0.055	5.525ValSer: 5.525 ± 0.067	4.225ValThr: 4.225 ± 0.05	5.012ValVal: 5.012 ± 0.057	0.596ValTrp: 0.596 ± 0.018	2.297ValTyr: 2.297 ± 0.038	0.002ValXaa: 0.002 ± 0.001
Trp	0.509TrpAla: 0.509 ± 0.019	0.223TrpCys: 0.223 ± 0.012	0.607TrpAsp: 0.607 ± 0.021	0.544TrpGlu: 0.544 ± 0.02	0.385TrpPhe: 0.385 ± 0.018	0.407TrpGly: 0.407 ± 0.016	0.294TrpHis: 0.294 ± 0.014	0.619TrpIle: 0.619 ± 0.019	0.579TrpLys: 0.579 ± 0.022	1.086TrpLeu: 1.086 ± 0.026	0.288TrpMet: 0.288 ± 0.013	0.523TrpAsn: 0.523 ± 0.018	0.346TrpPro: 0.346 ± 0.015	0.398TrpGln: 0.398 ± 0.015	0.704TrpArg: 0.704 ± 0.021	0.757TrpSer: 0.757 ± 0.022	0.473TrpThr: 0.473 ± 0.018	0.596TrpVal: 0.596 ± 0.019	0.119TrpTrp: 0.119 ± 0.009	0.382TrpTyr: 0.382 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.124TyrAla: 2.124 ± 0.037	0.735TyrCys: 0.735 ± 0.025	2.165TyrAsp: 2.165 ± 0.037	1.859TyrGlu: 1.859 ± 0.033	1.439TyrPhe: 1.439 ± 0.032	2.042TyrGly: 2.042 ± 0.036	0.894TyrHis: 0.894 ± 0.023	2.004TyrIle: 2.004 ± 0.04	1.908TyrLys: 1.908 ± 0.038	3.202TyrLeu: 3.202 ± 0.05	0.928TyrMet: 0.928 ± 0.026	1.718TyrAsn: 1.718 ± 0.032	1.295TyrPro: 1.295 ± 0.031	1.046TyrGln: 1.046 ± 0.025	2.226TyrArg: 2.226 ± 0.038	2.465TyrSer: 2.465 ± 0.042	1.891TyrThr: 1.891 ± 0.033	2.38TyrVal: 2.38 ± 0.035	0.336TyrTrp: 0.336 ± 0.013	1.229TyrTyr: 1.229 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.006

Statistics based on 2856 proteins (1634980 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---