Halovenus aranensis
Average proteome isoelectric point is 4.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3354 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8YLL0|A0A1G8YLL0_9EURY Dihydroneopterin aldolase OS=Halovenus aranensis OX=890420 GN=mptD PE=3 SV=1
MM1 pKa = 7.3 NPEE4 pKa = 4.75 DD5 pKa = 4.46 FPTPAVDD12 pKa = 3.81 VEE14 pKa = 4.89 SVAPEE19 pKa = 4.04 TLRR22 pKa = 11.84 GRR24 pKa = 11.84 IDD26 pKa = 3.24 ANEE29 pKa = 3.97 RR30 pKa = 11.84 VTLLDD35 pKa = 3.24 ARR37 pKa = 11.84 MEE39 pKa = 4.17 SEE41 pKa = 3.78 YY42 pKa = 11.2 DD43 pKa = 2.91 EE44 pKa = 4.15 WHH46 pKa = 6.46 IDD48 pKa = 3.66 GPSVEE53 pKa = 5.27 SINIPYY59 pKa = 10.0 FHH61 pKa = 7.18 FLDD64 pKa = 5.52 DD65 pKa = 4.36 EE66 pKa = 4.69 VDD68 pKa = 3.86 DD69 pKa = 5.24 DD70 pKa = 4.72 VLAEE74 pKa = 4.16 VPDD77 pKa = 3.97 DD78 pKa = 4.15 RR79 pKa = 11.84 EE80 pKa = 4.37 VTVLCAKK87 pKa = 10.37 GGASEE92 pKa = 4.4 YY93 pKa = 10.86 VAGTLAEE100 pKa = 4.3 LGYY103 pKa = 10.66 EE104 pKa = 4.0 VDD106 pKa = 3.77 HH107 pKa = 8.28 LEE109 pKa = 4.09 EE110 pKa = 5.17 GMNGWATIYY119 pKa = 10.74 DD120 pKa = 3.73 AVEE123 pKa = 3.74 VTGYY127 pKa = 10.94 DD128 pKa = 3.28 GAGTLLQYY136 pKa = 10.06 QRR138 pKa = 11.84 PSSGCLGYY146 pKa = 11.15 LLVDD150 pKa = 3.89 GGEE153 pKa = 4.1 AAVIDD158 pKa = 4.42 PLRR161 pKa = 11.84 AFTDD165 pKa = 4.04 RR166 pKa = 11.84 YY167 pKa = 10.49 LADD170 pKa = 4.68 ADD172 pKa = 4.52 DD173 pKa = 5.2 LGVDD177 pKa = 4.41 LKK179 pKa = 11.32 YY180 pKa = 11.02 ALDD183 pKa = 3.64 THH185 pKa = 6.8 IHH187 pKa = 6.68 ADD189 pKa = 3.7 HH190 pKa = 6.89 ISGVRR195 pKa = 11.84 DD196 pKa = 3.4 LDD198 pKa = 3.87 AEE200 pKa = 4.39 GVEE203 pKa = 4.6 GVIPEE208 pKa = 4.09 AAVDD212 pKa = 3.7 RR213 pKa = 11.84 GVTYY217 pKa = 10.86 AEE219 pKa = 4.38 EE220 pKa = 4.18 LTTAADD226 pKa = 3.77 GDD228 pKa = 4.45 TIAVGDD234 pKa = 3.84 ATIEE238 pKa = 4.31 TVHH241 pKa = 6.22 TPGHH245 pKa = 4.77 TTGMTSYY252 pKa = 10.98 LVDD255 pKa = 4.36 DD256 pKa = 4.7 SFLATGDD263 pKa = 3.76 GLFVEE268 pKa = 5.56 SVARR272 pKa = 11.84 PDD274 pKa = 4.52 LEE276 pKa = 4.65 EE277 pKa = 6.06 GDD279 pKa = 4.23 EE280 pKa = 4.34 GAPDD284 pKa = 4.07 AARR287 pKa = 11.84 MLYY290 pKa = 7.91 EE291 pKa = 4.06 TLQEE295 pKa = 4.78 RR296 pKa = 11.84 ILALPDD302 pKa = 3.1 EE303 pKa = 4.93 TIVGGAHH310 pKa = 7.0 ASDD313 pKa = 3.58 AAAAAEE319 pKa = 4.68 DD320 pKa = 4.01 GTYY323 pKa = 8.3 TAPLGDD329 pKa = 4.85 LVAEE333 pKa = 4.32 MDD335 pKa = 4.27 ALTMDD340 pKa = 3.9 EE341 pKa = 4.43 EE342 pKa = 4.73 AFVDD346 pKa = 5.27 LVLADD351 pKa = 3.95 MPPRR355 pKa = 11.84 PANYY359 pKa = 10.08 EE360 pKa = 3.96 EE361 pKa = 5.46 IIATNLGQNSVDD373 pKa = 3.71 DD374 pKa = 4.51 EE375 pKa = 4.46 EE376 pKa = 6.51 AFTLEE381 pKa = 4.99 LGPNNCAASQEE392 pKa = 4.43 SLAGDD397 pKa = 3.77
Molecular weight: 42.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.643
IPC_protein 3.681
Toseland 3.452
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.579
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 3.999
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.795
Patrickios 1.354
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.725
Protein with the highest isoelectric point:
>tr|A0A1G8YV29|A0A1G8YV29_9EURY Protein-export membrane protein SecF OS=Halovenus aranensis OX=890420 GN=secF PE=3 SV=1
MM1 pKa = 7.28 ARR3 pKa = 11.84 SFYY6 pKa = 11.12 SHH8 pKa = 7.07 IRR10 pKa = 11.84 DD11 pKa = 3.27 AWKK14 pKa = 10.73 DD15 pKa = 3.42 PDD17 pKa = 4.17 DD18 pKa = 4.52 GKK20 pKa = 11.07 VAQLQWEE27 pKa = 4.48 RR28 pKa = 11.84 QQEE31 pKa = 4.02 WRR33 pKa = 11.84 DD34 pKa = 3.22 QGAIEE39 pKa = 4.73 RR40 pKa = 11.84 IEE42 pKa = 4.1 RR43 pKa = 11.84 PTRR46 pKa = 11.84 LDD48 pKa = 3.12 RR49 pKa = 11.84 ARR51 pKa = 11.84 SLGYY55 pKa = 9.43 KK56 pKa = 9.87 AKK58 pKa = 10.46 QGVVLARR65 pKa = 11.84 VSVRR69 pKa = 11.84 KK70 pKa = 10.02 GGSRR74 pKa = 11.84 KK75 pKa = 8.19 QRR77 pKa = 11.84 FTAGRR82 pKa = 11.84 RR83 pKa = 11.84 SARR86 pKa = 11.84 QGVNRR91 pKa = 11.84 LNRR94 pKa = 11.84 RR95 pKa = 11.84 KK96 pKa = 9.97 NIQRR100 pKa = 11.84 IAEE103 pKa = 4.08 EE104 pKa = 3.88 RR105 pKa = 11.84 CTRR108 pKa = 11.84 KK109 pKa = 8.44 FTNLRR114 pKa = 11.84 VLNSYY119 pKa = 8.44 WVGEE123 pKa = 4.4 DD124 pKa = 4.78 GSQKK128 pKa = 8.97 WFEE131 pKa = 4.62 VIMLDD136 pKa = 3.77 PEE138 pKa = 4.41 HH139 pKa = 6.93 PAIEE143 pKa = 4.75 NDD145 pKa = 5.08 DD146 pKa = 4.12 DD147 pKa = 5.67 LNWICDD153 pKa = 3.7 DD154 pKa = 3.61 THH156 pKa = 7.42 KK157 pKa = 10.93 NRR159 pKa = 11.84 AMRR162 pKa = 11.84 GLTSAGTQNRR172 pKa = 11.84 GLEE175 pKa = 4.37 SKK177 pKa = 11.04 GKK179 pKa = 8.02 GTEE182 pKa = 3.7 RR183 pKa = 11.84 TRR185 pKa = 11.84 PSLSSNKK192 pKa = 9.22 SRR194 pKa = 11.84 KK195 pKa = 8.98
Molecular weight: 22.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.589
IPC_protein 10.365
Toseland 10.877
ProMoST 10.643
Dawson 10.921
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.096
Grimsley 10.95
Solomon 11.096
Lehninger 11.067
Nozaki 10.847
DTASelect 10.643
Thurlkill 10.847
EMBOSS 11.286
Sillero 10.862
Patrickios 10.818
IPC_peptide 11.111
IPC2_peptide 9.545
IPC2.peptide.svr19 8.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3354
0
3354
979672
24
1886
292.1
31.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.136 ± 0.055
0.768 ± 0.016
8.247 ± 0.053
8.918 ± 0.056
3.318 ± 0.028
8.128 ± 0.041
2.021 ± 0.024
4.267 ± 0.033
1.954 ± 0.028
8.963 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.779 ± 0.017
2.465 ± 0.023
4.452 ± 0.027
3.097 ± 0.024
6.248 ± 0.043
5.671 ± 0.037
6.9 ± 0.037
8.846 ± 0.047
1.107 ± 0.015
2.714 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here