Wenling crustacean virus 10

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alphacrustrhavirus; Wenling alphacrustrhavirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNA1|A0A1L3KNA1_9VIRU Uncharacterized protein OS=Wenling crustacean virus 10 OX=1923479 PE=4 SV=1
MM1 pKa = 6.95TRR3 pKa = 11.84KK4 pKa = 10.21KK5 pKa = 10.49IQDD8 pKa = 4.05PCNMADD14 pKa = 3.59PAYY17 pKa = 9.9QALSGLPSRR26 pKa = 11.84LVDD29 pKa = 3.67VQDD32 pKa = 4.82KK33 pKa = 10.59GWSDD37 pKa = 4.3DD38 pKa = 3.71SLKK41 pKa = 10.82KK42 pKa = 10.44YY43 pKa = 10.15IKK45 pKa = 10.07SRR47 pKa = 11.84YY48 pKa = 8.42SLPGEE53 pKa = 4.23DD54 pKa = 6.09DD55 pKa = 5.33RR56 pKa = 11.84ITLCKK61 pKa = 10.44LFLQAYY67 pKa = 10.02CYY69 pKa = 11.21GNWQNFKK76 pKa = 10.64LSEE79 pKa = 4.1IVHH82 pKa = 6.61ALLLITPTRR91 pKa = 11.84TEE93 pKa = 3.82KK94 pKa = 10.73KK95 pKa = 9.56YY96 pKa = 10.75LALFACFKK104 pKa = 9.88TKK106 pKa = 10.22QSPLIDD112 pKa = 3.62LSGAKK117 pKa = 9.17VQPADD122 pKa = 4.03DD123 pKa = 4.27ATVSDD128 pKa = 4.84PKK130 pKa = 10.61TSQKK134 pKa = 11.34DD135 pKa = 3.54DD136 pKa = 3.78TNDD139 pKa = 3.31LQGLVGEE146 pKa = 5.18HH147 pKa = 6.96DD148 pKa = 4.6WIDD151 pKa = 3.91DD152 pKa = 3.62DD153 pKa = 5.93DD154 pKa = 6.23NIVTGLPRR162 pKa = 11.84NTSSFYY168 pKa = 11.06KK169 pKa = 10.85GMLEE173 pKa = 5.36ALDD176 pKa = 4.58GDD178 pKa = 4.46DD179 pKa = 4.84KK180 pKa = 11.12IAAIRR185 pKa = 11.84FCCFLAWVCFRR196 pKa = 11.84TVVKK200 pKa = 10.2EE201 pKa = 4.11VSSVRR206 pKa = 11.84AYY208 pKa = 10.82LGRR211 pKa = 11.84AEE213 pKa = 4.1NFSRR217 pKa = 11.84LVTNLTDD224 pKa = 3.97FEE226 pKa = 4.74LPEE229 pKa = 4.35KK230 pKa = 10.94VPLPSKK236 pKa = 10.84IMLEE240 pKa = 4.27KK241 pKa = 10.33IAITMGKK248 pKa = 7.83GTASVRR254 pKa = 11.84EE255 pKa = 4.28LISWAVYY262 pKa = 10.27VKK264 pKa = 10.34VHH266 pKa = 6.74SDD268 pKa = 3.07NDD270 pKa = 3.62DD271 pKa = 3.62DD272 pKa = 4.17VRR274 pKa = 11.84MMEE277 pKa = 4.69GGCLTHH283 pKa = 8.11LSEE286 pKa = 4.94NGIGLVGLVDD296 pKa = 3.73KK297 pKa = 11.1VAGVFSVSIEE307 pKa = 4.33EE308 pKa = 4.05ILSLILTSHH317 pKa = 6.13TMNSVIRR324 pKa = 11.84LADD327 pKa = 4.71FIKK330 pKa = 10.7HH331 pKa = 4.98VVPKK335 pKa = 9.96VKK337 pKa = 9.13SWKK340 pKa = 7.53WSRR343 pKa = 11.84VIDD346 pKa = 4.07DD347 pKa = 3.63QCFSSLKK354 pKa = 10.28ILDD357 pKa = 4.62NIVLSAYY364 pKa = 10.19LVALVTEE371 pKa = 4.78ADD373 pKa = 3.98AGNDD377 pKa = 3.08MWNMVVFEE385 pKa = 3.96QHH387 pKa = 6.64LPNTTKK393 pKa = 10.76NQALRR398 pKa = 11.84WAKK401 pKa = 10.06KK402 pKa = 8.87YY403 pKa = 10.16VVASSEE409 pKa = 4.1HH410 pKa = 5.58DD411 pKa = 3.35QKK413 pKa = 10.17TGAWVSEE420 pKa = 3.93KK421 pKa = 10.23HH422 pKa = 5.52RR423 pKa = 11.84KK424 pKa = 7.27IWAYY428 pKa = 10.63GEE430 pKa = 5.11DD431 pKa = 4.43DD432 pKa = 4.51DD433 pKa = 6.41EE434 pKa = 6.93SDD436 pKa = 3.63NEE438 pKa = 4.12YY439 pKa = 11.16SGLII443 pKa = 3.74

Molecular weight: 49.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN39|A0A1L3KN39_9VIRU Uncharacterized protein OS=Wenling crustacean virus 10 OX=1923479 PE=4 SV=1
MM1 pKa = 6.96QCRR4 pKa = 11.84FRR6 pKa = 11.84KK7 pKa = 10.08NKK9 pKa = 9.23TPEE12 pKa = 4.0KK13 pKa = 10.26LDD15 pKa = 3.16IMSNLVIKK23 pKa = 10.52LCAEE27 pKa = 3.98GTIVGTPAHH36 pKa = 6.5SWAQIASTIVDD47 pKa = 4.36ALFKK51 pKa = 11.06DD52 pKa = 4.38CGFSPKK58 pKa = 9.64IMAQIIVLLHH68 pKa = 5.85EE69 pKa = 4.39SLRR72 pKa = 11.84DD73 pKa = 3.69KK74 pKa = 11.1VQEE77 pKa = 4.1TPVRR81 pKa = 11.84RR82 pKa = 11.84TIISLKK88 pKa = 10.94DD89 pKa = 3.59EE90 pKa = 4.5IEE92 pKa = 4.33KK93 pKa = 10.64EE94 pKa = 4.25WVTKK98 pKa = 9.35RR99 pKa = 11.84TYY101 pKa = 9.6NCKK104 pKa = 10.03LVAHH108 pKa = 6.56TSFPYY113 pKa = 10.73SPDD116 pKa = 3.18LNGFLKK122 pKa = 10.65KK123 pKa = 10.69LNLEE127 pKa = 4.06LSGEE131 pKa = 4.34GKK133 pKa = 10.39EE134 pKa = 4.32GTVVVSLRR142 pKa = 11.84LQGEE146 pKa = 4.45LRR148 pKa = 11.84CKK150 pKa = 8.18PTNYY154 pKa = 10.11DD155 pKa = 3.21PQTIQDD161 pKa = 4.0LRR163 pKa = 11.84FVPLPPVGKK172 pKa = 10.39VLTEE176 pKa = 4.0QISKK180 pKa = 10.43LRR182 pKa = 11.84AKK184 pKa = 9.26TKK186 pKa = 10.62NQIEE190 pKa = 4.4VPKK193 pKa = 10.87DD194 pKa = 2.99HH195 pKa = 6.73GQDD198 pKa = 3.24EE199 pKa = 5.05GKK201 pKa = 10.51GASSKK206 pKa = 10.84GRR208 pKa = 11.84LKK210 pKa = 11.21GLFSGLKK217 pKa = 10.12AKK219 pKa = 9.98EE220 pKa = 3.77

Molecular weight: 24.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3799

220

2121

759.8

85.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.764 ± 0.512

1.764 ± 0.296

5.212 ± 0.848

6.554 ± 0.822

3.948 ± 0.314

5.791 ± 0.21

2.316 ± 0.18

6.002 ± 0.552

7.265 ± 1.296

11.345 ± 0.46

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.343

3.343 ± 0.203

4.606 ± 0.302

3.948 ± 0.278

4.396 ± 0.555

8.95 ± 0.383

6.712 ± 0.552

5.844 ± 0.429

2.211 ± 0.326

2.843 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski