Torque teno virus 5
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q99AR2|Q99AR2_9VIRU Orf2 OS=Torque teno virus 5 OX=687344 PE=4 SV=1
MM1 pKa = 7.51 GKK3 pKa = 9.73 ALKK6 pKa = 10.2 KK7 pKa = 9.37 ATPLTMFFGRR17 pKa = 11.84 VYY19 pKa = 10.22 RR20 pKa = 11.84 HH21 pKa = 5.62 QKK23 pKa = 10.3 RR24 pKa = 11.84 KK25 pKa = 9.49 VLLSLVHH32 pKa = 6.47 TPQKK36 pKa = 9.78 TPSSMNRR43 pKa = 11.84 WSPPAHH49 pKa = 7.14 DD50 pKa = 3.44 ARR52 pKa = 11.84 GIEE55 pKa = 4.17 RR56 pKa = 11.84 QWYY59 pKa = 7.26 EE60 pKa = 4.34 CVWRR64 pKa = 11.84 SHH66 pKa = 6.67 AACCGCGDD74 pKa = 4.16 FVGHH78 pKa = 6.76 INDD81 pKa = 3.67 LAHH84 pKa = 7.44 RR85 pKa = 11.84 FGRR88 pKa = 11.84 PPGSRR93 pKa = 11.84 PPTTPQPPAVRR104 pKa = 11.84 ALPALPAPEE113 pKa = 4.41 PWSGAGGDD121 pKa = 3.91 AGAGGDD127 pKa = 3.77 GAAGRR132 pKa = 11.84 GGGDD136 pKa = 3.23 GDD138 pKa = 4.25 AGDD141 pKa = 4.25 VADD144 pKa = 5.55 EE145 pKa = 4.81 DD146 pKa = 4.86 LLDD149 pKa = 4.37 AVDD152 pKa = 3.91 LAEE155 pKa = 4.18
Molecular weight: 16.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.867
IPC2_protein 6.927
IPC_protein 7.146
Toseland 6.942
ProMoST 7.263
Dawson 7.732
Bjellqvist 7.746
Wikipedia 7.658
Rodwell 7.702
Grimsley 7.015
Solomon 7.849
Lehninger 7.878
Nozaki 8.141
DTASelect 7.878
Thurlkill 7.907
EMBOSS 7.995
Sillero 8.185
Patrickios 4.609
IPC_peptide 7.849
IPC2_peptide 7.512
IPC2.peptide.svr19 7.394
Protein with the highest isoelectric point:
>tr|Q99AR2|Q99AR2_9VIRU Orf2 OS=Torque teno virus 5 OX=687344 PE=4 SV=1
MM1 pKa = 7.72 LMLAKK6 pKa = 9.96 KK7 pKa = 10.38 RR8 pKa = 11.84 IIIPSLKK15 pKa = 8.83 TRR17 pKa = 11.84 PSKK20 pKa = 10.44 KK21 pKa = 9.58 HH22 pKa = 5.16 YY23 pKa = 10.16 IKK25 pKa = 10.58 IKK27 pKa = 10.09 IGAPKK32 pKa = 10.3 LFEE35 pKa = 5.04 DD36 pKa = 3.05 KK37 pKa = 10.15 WYY39 pKa = 10.25 SQRR42 pKa = 11.84 DD43 pKa = 3.58 LCDD46 pKa = 3.02 TTLVVIEE53 pKa = 4.5 ATVADD58 pKa = 4.09 LQYY61 pKa = 11.06 PFGSPLTNSICCNFQVLNSNYY82 pKa = 10.57 DD83 pKa = 3.38 NALSTLIGTQGDD95 pKa = 3.74 KK96 pKa = 11.28 DD97 pKa = 3.51 RR98 pKa = 11.84 DD99 pKa = 3.41 QCYY102 pKa = 10.3 KK103 pKa = 10.75 FLLNTITYY111 pKa = 9.08 YY112 pKa = 9.67 NTSQTLAQLNKK123 pKa = 9.92 FLPTTNTTQLSQNNAQNSINPSNTTEE149 pKa = 3.74 NTYY152 pKa = 11.12 DD153 pKa = 3.35 SRR155 pKa = 11.84 NTEE158 pKa = 3.41 IHH160 pKa = 6.6 NNTLYY165 pKa = 11.09 GGAAYY170 pKa = 10.14 YY171 pKa = 9.33 KK172 pKa = 10.57 QNGSNTNTLPPHH184 pKa = 6.66 KK185 pKa = 9.55 MKK187 pKa = 10.48 EE188 pKa = 3.98 ANKK191 pKa = 9.59 TYY193 pKa = 9.58 TAAAKK198 pKa = 10.24 KK199 pKa = 10.15 ILSNWNFTNMSTSSSADD216 pKa = 3.52 LQGLNYY222 pKa = 9.68 FDD224 pKa = 5.71 YY225 pKa = 8.65 YY226 pKa = 11.15 TGMFSSIFLASGRR239 pKa = 11.84 SNWEE243 pKa = 3.63 VKK245 pKa = 10.52 GSYY248 pKa = 9.75 TDD250 pKa = 2.89 ITYY253 pKa = 11.05 NPLVDD258 pKa = 3.66 KK259 pKa = 11.61 GEE261 pKa = 4.46 GNMIWIDD268 pKa = 4.22 WITKK272 pKa = 9.87 GDD274 pKa = 3.87 TIYY277 pKa = 11.05 SEE279 pKa = 4.67 NKK281 pKa = 9.78 SKK283 pKa = 10.94 CLLRR287 pKa = 11.84 DD288 pKa = 3.29 LPMWALCYY296 pKa = 10.44 GYY298 pKa = 10.99 ADD300 pKa = 3.87 YY301 pKa = 11.32 VLKK304 pKa = 9.93 CTGISSIKK312 pKa = 9.53 NEE314 pKa = 3.42 ARR316 pKa = 11.84 IVMRR320 pKa = 11.84 CPYY323 pKa = 9.66 TYY325 pKa = 10.17 PQLVKK330 pKa = 10.8 HH331 pKa = 6.27 NNDD334 pKa = 2.62 NFGFVVYY341 pKa = 10.42 SDD343 pKa = 3.17 NFGRR347 pKa = 11.84 GRR349 pKa = 11.84 MPGGDD354 pKa = 3.64 PVPSTRR360 pKa = 11.84 NRR362 pKa = 11.84 VHH364 pKa = 6.63 WYY366 pKa = 8.13 ITITHH371 pKa = 4.63 QTEE374 pKa = 3.95 VLEE377 pKa = 5.43 CISQTGPFAYY387 pKa = 10.27 HH388 pKa = 5.62 SDD390 pKa = 3.19 EE391 pKa = 4.26 RR392 pKa = 11.84 KK393 pKa = 9.74 AVLTIKK399 pKa = 11.01 YY400 pKa = 9.18 NFRR403 pKa = 11.84 WKK405 pKa = 9.9 WGGNPVFQQILRR417 pKa = 11.84 DD418 pKa = 3.9 PCSGSPGSGPRR429 pKa = 11.84 RR430 pKa = 11.84 VPRR433 pKa = 11.84 SIQVDD438 pKa = 3.58 DD439 pKa = 4.86 PKK441 pKa = 11.22 YY442 pKa = 9.11 QTPEE446 pKa = 4.46 YY447 pKa = 9.44 IWHH450 pKa = 6.44 AWDD453 pKa = 3.81 FRR455 pKa = 11.84 RR456 pKa = 11.84 GLFSQKK462 pKa = 10.07 GIKK465 pKa = 9.54 RR466 pKa = 11.84 VSEE469 pKa = 3.96 QPTDD473 pKa = 3.08 VDD475 pKa = 4.27 LPTGRR480 pKa = 11.84 GKK482 pKa = 10.52 RR483 pKa = 11.84 PKK485 pKa = 9.69 RR486 pKa = 11.84 DD487 pKa = 2.94 TGGPQEE493 pKa = 4.14 QGQEE497 pKa = 4.18 EE498 pKa = 5.05 GSSSIRR504 pKa = 11.84 RR505 pKa = 11.84 VLQQWLHH512 pKa = 5.8 SSQEE516 pKa = 3.85 QSQEE520 pKa = 4.19 SEE522 pKa = 4.17 EE523 pKa = 4.17 EE524 pKa = 3.94 AAPTTLSQEE533 pKa = 4.03 LQKK536 pKa = 10.58 QLKK539 pKa = 8.04 QQQLMGKK546 pKa = 7.26 QLRR549 pKa = 11.84 EE550 pKa = 4.1 LSLQLAQVQAGGHH563 pKa = 5.81 LHH565 pKa = 7.38 PLLQCHH571 pKa = 6.21 AA572 pKa = 4.67
Molecular weight: 65.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.287
IPC2_protein 8.302
IPC_protein 8.156
Toseland 8.419
ProMoST 8.668
Dawson 8.975
Bjellqvist 9.019
Wikipedia 9.077
Rodwell 9.063
Grimsley 8.712
Solomon 9.033
Lehninger 9.004
Nozaki 9.107
DTASelect 8.858
Thurlkill 8.931
EMBOSS 9.107
Sillero 9.151
Patrickios 4.19
IPC_peptide 9.019
IPC2_peptide 7.819
IPC2.peptide.svr19 7.78
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
727
155
572
363.5
40.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.327 ± 3.153
2.063 ± 0.248
5.502 ± 1.074
3.989 ± 0.366
2.889 ± 0.148
8.116 ± 2.605
2.476 ± 0.669
4.539 ± 1.558
5.915 ± 0.98
8.116 ± 0.488
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.788 ± 0.071
5.502 ± 2.019
6.327 ± 1.916
6.052 ± 1.665
5.502 ± 1.074
7.29 ± 1.33
7.015 ± 1.508
4.127 ± 0.496
2.201 ± 0.182
4.264 ± 1.426
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here