Amino acid dipepetide frequency for Sphingomonas sp. FARSPH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.35AlaAla: 23.35 ± 0.255
1.121AlaCys: 1.121 ± 0.037
8.738AlaAsp: 8.738 ± 0.104
7.064AlaGlu: 7.064 ± 0.102
4.428AlaPhe: 4.428 ± 0.064
12.909AlaGly: 12.909 ± 0.15
2.574AlaHis: 2.574 ± 0.05
7.39AlaIle: 7.39 ± 0.096
3.823AlaLys: 3.823 ± 0.071
15.138AlaLeu: 15.138 ± 0.157
4.166AlaMet: 4.166 ± 0.074
3.205AlaAsn: 3.205 ± 0.066
7.298AlaPro: 7.298 ± 0.107
4.825AlaGln: 4.825 ± 0.08
11.28AlaArg: 11.28 ± 0.126
6.399AlaSer: 6.399 ± 0.09
8.1AlaThr: 8.1 ± 0.13
9.551AlaVal: 9.551 ± 0.109
1.849AlaTrp: 1.849 ± 0.046
2.741AlaTyr: 2.741 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.965CysAla: 0.965 ± 0.033
0.077CysCys: 0.077 ± 0.008
0.466CysAsp: 0.466 ± 0.021
0.299CysGlu: 0.299 ± 0.018
0.239CysPhe: 0.239 ± 0.014
0.813CysGly: 0.813 ± 0.03
0.184CysHis: 0.184 ± 0.013
0.326CysIle: 0.326 ± 0.016
0.12CysLys: 0.12 ± 0.012
0.601CysLeu: 0.601 ± 0.024
0.123CysMet: 0.123 ± 0.01
0.162CysAsn: 0.162 ± 0.012
0.44CysPro: 0.44 ± 0.021
0.191CysGln: 0.191 ± 0.014
0.549CysArg: 0.549 ± 0.021
0.369CysSer: 0.369 ± 0.02
0.407CysThr: 0.407 ± 0.023
0.511CysVal: 0.511 ± 0.025
0.104CysTrp: 0.104 ± 0.009
0.143CysTyr: 0.143 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
9.283AspAla: 9.283 ± 0.103
0.365AspCys: 0.365 ± 0.022
3.505AspAsp: 3.505 ± 0.07
2.992AspGlu: 2.992 ± 0.059
1.897AspPhe: 1.897 ± 0.046
5.955AspGly: 5.955 ± 0.082
1.358AspHis: 1.358 ± 0.043
2.617AspIle: 2.617 ± 0.055
1.445AspLys: 1.445 ± 0.04
5.889AspLeu: 5.889 ± 0.084
1.24AspMet: 1.24 ± 0.039
1.181AspAsn: 1.181 ± 0.039
4.078AspPro: 4.078 ± 0.069
1.87AspGln: 1.87 ± 0.042
5.488AspArg: 5.488 ± 0.085
1.933AspSer: 1.933 ± 0.046
3.095AspThr: 3.095 ± 0.053
4.433AspVal: 4.433 ± 0.074
1.175AspTrp: 1.175 ± 0.029
1.653AspTyr: 1.653 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
7.102GluAla: 7.102 ± 0.106
0.239GluCys: 0.239 ± 0.016
2.372GluAsp: 2.372 ± 0.058
2.272GluGlu: 2.272 ± 0.063
1.149GluPhe: 1.149 ± 0.036
3.955GluGly: 3.955 ± 0.069
0.977GluHis: 0.977 ± 0.029
2.28GluIle: 2.28 ± 0.059
1.465GluLys: 1.465 ± 0.05
4.06GluLeu: 4.06 ± 0.069
1.167GluMet: 1.167 ± 0.039
0.987GluAsn: 0.987 ± 0.035
2.269GluPro: 2.269 ± 0.053
1.732GluGln: 1.732 ± 0.038
4.615GluArg: 4.615 ± 0.073
1.799GluSer: 1.799 ± 0.043
2.93GluThr: 2.93 ± 0.054
3.268GluVal: 3.268 ± 0.065
0.64GluTrp: 0.64 ± 0.028
0.769GluTyr: 0.769 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
4.965PheAla: 4.965 ± 0.071
0.241PheCys: 0.241 ± 0.016
2.572PheAsp: 2.572 ± 0.05
1.648PheGlu: 1.648 ± 0.041
1.171PhePhe: 1.171 ± 0.035
3.458PheGly: 3.458 ± 0.059
0.703PheHis: 0.703 ± 0.027
1.155PheIle: 1.155 ± 0.041
0.692PheLys: 0.692 ± 0.028
2.913PheLeu: 2.913 ± 0.065
0.621PheMet: 0.621 ± 0.024
0.879PheAsn: 0.879 ± 0.031
1.363PhePro: 1.363 ± 0.038
0.84PheGln: 0.84 ± 0.027
2.269PheArg: 2.269 ± 0.047
1.674PheSer: 1.674 ± 0.041
1.995PheThr: 1.995 ± 0.046
2.757PheVal: 2.757 ± 0.054
0.491PheTrp: 0.491 ± 0.021
0.84PheTyr: 0.84 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
10.977GlyAla: 10.977 ± 0.139
0.836GlyCys: 0.836 ± 0.032
5.319GlyAsp: 5.319 ± 0.081
4.491GlyGlu: 4.491 ± 0.074
3.538GlyPhe: 3.538 ± 0.062
8.675GlyGly: 8.675 ± 0.17
1.879GlyHis: 1.879 ± 0.044
4.463GlyIle: 4.463 ± 0.06
2.777GlyLys: 2.777 ± 0.055
8.598GlyLeu: 8.598 ± 0.096
2.287GlyMet: 2.287 ± 0.05
2.205GlyAsn: 2.205 ± 0.046
3.643GlyPro: 3.643 ± 0.066
3.042GlyGln: 3.042 ± 0.055
7.223GlyArg: 7.223 ± 0.096
4.604GlySer: 4.604 ± 0.09
5.304GlyThr: 5.304 ± 0.15
6.72GlyVal: 6.72 ± 0.084
1.737GlyTrp: 1.737 ± 0.042
2.458GlyTyr: 2.458 ± 0.057
0.0GlyXaa: 0.0 ± 0.0
His
2.764HisAla: 2.764 ± 0.054
0.179HisCys: 0.179 ± 0.014
1.38HisAsp: 1.38 ± 0.039
0.893HisGlu: 0.893 ± 0.03
0.717HisPhe: 0.717 ± 0.026
2.031HisGly: 2.031 ± 0.044
0.596HisHis: 0.596 ± 0.026
0.79HisIle: 0.79 ± 0.029
0.367HisLys: 0.367 ± 0.018
1.877HisLeu: 1.877 ± 0.042
0.397HisMet: 0.397 ± 0.019
0.416HisAsn: 0.416 ± 0.023
1.287HisPro: 1.287 ± 0.037
0.529HisGln: 0.529 ± 0.022
1.655HisArg: 1.655 ± 0.047
0.738HisSer: 0.738 ± 0.026
0.716HisThr: 0.716 ± 0.026
1.502HisVal: 1.502 ± 0.039
0.332HisTrp: 0.332 ± 0.017
0.506HisTyr: 0.506 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
8.386IleAla: 8.386 ± 0.088
0.338IleCys: 0.338 ± 0.019
3.978IleAsp: 3.978 ± 0.061
2.945IleGlu: 2.945 ± 0.058
1.298IlePhe: 1.298 ± 0.035
5.219IleGly: 5.219 ± 0.079
0.787IleHis: 0.787 ± 0.027
1.554IleIle: 1.554 ± 0.041
1.007IleLys: 1.007 ± 0.034
3.642IleLeu: 3.642 ± 0.068
0.722IleMet: 0.722 ± 0.027
1.127IleAsn: 1.127 ± 0.036
2.059IlePro: 2.059 ± 0.046
1.046IleGln: 1.046 ± 0.031
3.165IleArg: 3.165 ± 0.06
1.88IleSer: 1.88 ± 0.043
2.343IleThr: 2.343 ± 0.049
4.531IleVal: 4.531 ± 0.067
0.488IleTrp: 0.488 ± 0.021
0.876IleTyr: 0.876 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.754LysAla: 3.754 ± 0.062
0.097LysCys: 0.097 ± 0.01
1.311LysAsp: 1.311 ± 0.045
0.903LysGlu: 0.903 ± 0.035
0.643LysPhe: 0.643 ± 0.028
2.358LysGly: 2.358 ± 0.054
0.397LysHis: 0.397 ± 0.022
1.085LysIle: 1.085 ± 0.036
0.833LysLys: 0.833 ± 0.037
2.632LysLeu: 2.632 ± 0.059
0.616LysMet: 0.616 ± 0.025
0.556LysAsn: 0.556 ± 0.023
1.804LysPro: 1.804 ± 0.041
0.781LysGln: 0.781 ± 0.025
2.076LysArg: 2.076 ± 0.05
1.263LysSer: 1.263 ± 0.036
1.567LysThr: 1.567 ± 0.044
1.917LysVal: 1.917 ± 0.05
0.281LysTrp: 0.281 ± 0.017
0.498LysTyr: 0.498 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.355LeuAla: 15.355 ± 0.155
0.732LeuCys: 0.732 ± 0.03
6.311LeuAsp: 6.311 ± 0.088
3.862LeuGlu: 3.862 ± 0.075
3.454LeuPhe: 3.454 ± 0.063
8.282LeuGly: 8.282 ± 0.094
1.797LeuHis: 1.797 ± 0.047
4.499LeuIle: 4.499 ± 0.071
2.678LeuLys: 2.678 ± 0.057
9.225LeuLeu: 9.225 ± 0.141
2.024LeuMet: 2.024 ± 0.042
2.133LeuAsn: 2.133 ± 0.049
5.828LeuPro: 5.828 ± 0.083
2.314LeuGln: 2.314 ± 0.052
7.085LeuArg: 7.085 ± 0.094
5.6LeuSer: 5.6 ± 0.085
5.836LeuThr: 5.836 ± 0.09
7.588LeuVal: 7.588 ± 0.091
1.216LeuTrp: 1.216 ± 0.035
2.025LeuTyr: 2.025 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
3.251MetAla: 3.251 ± 0.068
0.144MetCys: 0.144 ± 0.01
0.976MetAsp: 0.976 ± 0.033
0.84MetGlu: 0.84 ± 0.031
0.6MetPhe: 0.6 ± 0.023
1.712MetGly: 1.712 ± 0.045
0.426MetHis: 0.426 ± 0.021
1.276MetIle: 1.276 ± 0.031
0.748MetLys: 0.748 ± 0.026
2.646MetLeu: 2.646 ± 0.051
0.643MetMet: 0.643 ± 0.027
0.581MetAsn: 0.581 ± 0.028
1.517MetPro: 1.517 ± 0.042
0.634MetGln: 0.634 ± 0.026
1.882MetArg: 1.882 ± 0.039
1.226MetSer: 1.226 ± 0.035
1.857MetThr: 1.857 ± 0.038
1.589MetVal: 1.589 ± 0.036
0.208MetTrp: 0.208 ± 0.014
0.263MetTyr: 0.263 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
3.097AsnAla: 3.097 ± 0.058
0.184AsnCys: 0.184 ± 0.012
1.259AsnAsp: 1.259 ± 0.034
0.847AsnGlu: 0.847 ± 0.025
0.805AsnPhe: 0.805 ± 0.029
2.279AsnGly: 2.279 ± 0.053
0.459AsnHis: 0.459 ± 0.022
1.074AsnIle: 1.074 ± 0.033
0.525AsnLys: 0.525 ± 0.023
2.297AsnLeu: 2.297 ± 0.053
0.436AsnMet: 0.436 ± 0.019
0.594AsnAsn: 0.594 ± 0.03
1.567AsnPro: 1.567 ± 0.036
0.692AsnGln: 0.692 ± 0.032
1.74AsnArg: 1.74 ± 0.038
0.996AsnSer: 0.996 ± 0.036
1.179AsnThr: 1.179 ± 0.04
1.849AsnVal: 1.849 ± 0.044
0.371AsnTrp: 0.371 ± 0.019
0.663AsnTyr: 0.663 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
8.401ProAla: 8.401 ± 0.115
0.298ProCys: 0.298 ± 0.019
3.984ProAsp: 3.984 ± 0.067
2.796ProGlu: 2.796 ± 0.059
1.891ProPhe: 1.891 ± 0.046
5.058ProGly: 5.058 ± 0.073
1.064ProHis: 1.064 ± 0.035
2.572ProIle: 2.572 ± 0.056
1.326ProLys: 1.326 ± 0.035
5.038ProLeu: 5.038 ± 0.079
1.196ProMet: 1.196 ± 0.039
1.188ProAsn: 1.188 ± 0.028
3.143ProPro: 3.143 ± 0.089
1.709ProGln: 1.709 ± 0.038
3.45ProArg: 3.45 ± 0.069
2.62ProSer: 2.62 ± 0.054
3.002ProThr: 3.002 ± 0.079
4.558ProVal: 4.558 ± 0.07
0.667ProTrp: 0.667 ± 0.025
1.17ProTyr: 1.17 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
4.541GlnAla: 4.541 ± 0.071
0.201GlnCys: 0.201 ± 0.015
1.376GlnAsp: 1.376 ± 0.037
1.014GlnGlu: 1.014 ± 0.03
1.02GlnPhe: 1.02 ± 0.029
2.537GlnGly: 2.537 ± 0.052
0.554GlnHis: 0.554 ± 0.023
1.526GlnIle: 1.526 ± 0.043
0.738GlnLys: 0.738 ± 0.029
2.996GlnLeu: 2.996 ± 0.063
0.741GlnMet: 0.741 ± 0.024
0.706GlnAsn: 0.706 ± 0.026
1.902GlnPro: 1.902 ± 0.051
1.252GlnGln: 1.252 ± 0.04
2.728GlnArg: 2.728 ± 0.049
1.499GlnSer: 1.499 ± 0.039
1.795GlnThr: 1.795 ± 0.046
2.29GlnVal: 2.29 ± 0.05
0.418GlnTrp: 0.418 ± 0.02
0.597GlnTyr: 0.597 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
10.207ArgAla: 10.207 ± 0.126
0.527ArgCys: 0.527 ± 0.023
4.957ArgAsp: 4.957 ± 0.076
3.631ArgGlu: 3.631 ± 0.064
3.203ArgPhe: 3.203 ± 0.058
5.668ArgGly: 5.668 ± 0.093
1.878ArgHis: 1.878 ± 0.042
4.265ArgIle: 4.265 ± 0.075
1.744ArgLys: 1.744 ± 0.044
8.763ArgLeu: 8.763 ± 0.124
2.083ArgMet: 2.083 ± 0.049
1.683ArgAsn: 1.683 ± 0.039
4.157ArgPro: 4.157 ± 0.072
2.557ArgGln: 2.557 ± 0.05
6.969ArgArg: 6.969 ± 0.111
3.567ArgSer: 3.567 ± 0.057
3.991ArgThr: 3.991 ± 0.055
5.457ArgVal: 5.457 ± 0.078
1.354ArgTrp: 1.354 ± 0.037
2.013ArgTyr: 2.013 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.146SerAla: 6.146 ± 0.088
0.342SerCys: 0.342 ± 0.02
2.819SerAsp: 2.819 ± 0.041
1.907SerGlu: 1.907 ± 0.038
1.842SerPhe: 1.842 ± 0.045
4.917SerGly: 4.917 ± 0.095
0.83SerHis: 0.83 ± 0.028
2.405SerIle: 2.405 ± 0.048
1.131SerLys: 1.131 ± 0.035
4.623SerLeu: 4.623 ± 0.073
0.99SerMet: 0.99 ± 0.03
1.212SerAsn: 1.212 ± 0.038
2.761SerPro: 2.761 ± 0.049
1.285SerGln: 1.285 ± 0.04
3.314SerArg: 3.314 ± 0.058
2.391SerSer: 2.391 ± 0.079
2.589SerThr: 2.589 ± 0.057
3.449SerVal: 3.449 ± 0.065
0.703SerTrp: 0.703 ± 0.025
1.231SerTyr: 1.231 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
7.345ThrAla: 7.345 ± 0.147
0.351ThrCys: 0.351 ± 0.019
3.124ThrAsp: 3.124 ± 0.054
2.067ThrGlu: 2.067 ± 0.042
1.81ThrPhe: 1.81 ± 0.044
5.805ThrGly: 5.805 ± 0.103
0.993ThrHis: 0.993 ± 0.031
3.36ThrIle: 3.36 ± 0.068
1.302ThrLys: 1.302 ± 0.037
6.427ThrLeu: 6.427 ± 0.134
1.274ThrMet: 1.274 ± 0.031
1.314ThrAsn: 1.314 ± 0.041
4.047ThrPro: 4.047 ± 0.096
1.748ThrGln: 1.748 ± 0.044
3.851ThrArg: 3.851 ± 0.058
2.642ThrSer: 2.642 ± 0.061
3.196ThrThr: 3.196 ± 0.07
4.443ThrVal: 4.443 ± 0.074
0.649ThrTrp: 0.649 ± 0.024
1.301ThrTyr: 1.301 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
11.591ValAla: 11.591 ± 0.12
0.491ValCys: 0.491 ± 0.023
4.67ValAsp: 4.67 ± 0.072
4.068ValGlu: 4.068 ± 0.067
2.213ValPhe: 2.213 ± 0.045
6.012ValGly: 6.012 ± 0.078
1.406ValHis: 1.406 ± 0.033
3.334ValIle: 3.334 ± 0.062
1.747ValLys: 1.747 ± 0.048
6.692ValLeu: 6.692 ± 0.075
1.562ValMet: 1.562 ± 0.037
1.757ValAsn: 1.757 ± 0.05
4.215ValPro: 4.215 ± 0.072
2.081ValGln: 2.081 ± 0.054
5.802ValArg: 5.802 ± 0.091
3.833ValSer: 3.833 ± 0.063
5.09ValThr: 5.09 ± 0.097
6.007ValVal: 6.007 ± 0.094
0.85ValTrp: 0.85 ± 0.028
1.409ValTyr: 1.409 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.445TrpAla: 1.445 ± 0.032
0.121TrpCys: 0.121 ± 0.011
0.715TrpAsp: 0.715 ± 0.027
0.488TrpGlu: 0.488 ± 0.02
0.578TrpPhe: 0.578 ± 0.022
0.955TrpGly: 0.955 ± 0.027
0.372TrpHis: 0.372 ± 0.019
0.656TrpIle: 0.656 ± 0.025
0.392TrpLys: 0.392 ± 0.02
1.726TrpLeu: 1.726 ± 0.044
0.34TrpMet: 0.34 ± 0.015
0.387TrpAsn: 0.387 ± 0.019
0.734TrpPro: 0.734 ± 0.026
0.626TrpGln: 0.626 ± 0.022
1.509TrpArg: 1.509 ± 0.04
0.777TrpSer: 0.777 ± 0.027
0.905TrpThr: 0.905 ± 0.029
0.819TrpVal: 0.819 ± 0.029
0.287TrpTrp: 0.287 ± 0.015
0.305TrpTyr: 0.305 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.025TyrAla: 3.025 ± 0.066
0.207TyrCys: 0.207 ± 0.014
1.616TyrAsp: 1.616 ± 0.056
1.019TyrGlu: 1.019 ± 0.03
0.756TyrPhe: 0.756 ± 0.031
2.178TyrGly: 2.178 ± 0.057
0.437TyrHis: 0.437 ± 0.018
0.767TyrIle: 0.767 ± 0.025
0.512TyrLys: 0.512 ± 0.024
2.062TyrLeu: 2.062 ± 0.044
0.389TyrMet: 0.389 ± 0.02
0.639TyrAsn: 0.639 ± 0.031
1.019TyrPro: 1.019 ± 0.031
0.676TyrGln: 0.676 ± 0.03
2.023TyrArg: 2.023 ± 0.049
1.075TyrSer: 1.075 ± 0.046
1.151TyrThr: 1.151 ± 0.059
1.593TyrVal: 1.593 ± 0.041
0.315TyrTrp: 0.315 ± 0.019
0.635TyrTyr: 0.635 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3408 proteins (1101842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski