Streptococcus phage Javan161

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A227|A0A4D6A227_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan161 OX=2548014 GN=Javan161_0015 PE=4 SV=1
MM1 pKa = 7.26ATLDD5 pKa = 3.84EE6 pKa = 4.51VLSFAKK12 pKa = 10.53GLADD16 pKa = 3.48TGQGVDD22 pKa = 3.78LDD24 pKa = 4.08NVYY27 pKa = 8.53GTQCVDD33 pKa = 3.5LPNWITTKK41 pKa = 10.75YY42 pKa = 10.5FGIALWGNAIDD53 pKa = 5.11LLDD56 pKa = 3.86SAAAQGMEE64 pKa = 4.21VVYY67 pKa = 10.3NAPGVNPRR75 pKa = 11.84AGAIFVMVTYY85 pKa = 10.75AHH87 pKa = 7.21GYY89 pKa = 7.86GHH91 pKa = 7.1TGLVIVTSDD100 pKa = 3.56GYY102 pKa = 10.61VLHH105 pKa = 7.03NIEE108 pKa = 4.75QNVDD112 pKa = 3.04GNADD116 pKa = 3.33ALYY119 pKa = 9.95IGGPARR125 pKa = 11.84YY126 pKa = 9.17VDD128 pKa = 4.39RR129 pKa = 11.84PFEE132 pKa = 5.2DD133 pKa = 3.37GTGYY137 pKa = 10.44ILGWFYY143 pKa = 10.78PPYY146 pKa = 10.83DD147 pKa = 3.7NTPVQEE153 pKa = 4.59IEE155 pKa = 3.86PSAPVVAQSDD165 pKa = 3.67GTYY168 pKa = 10.39VVNPEE173 pKa = 3.95TGTFTVRR180 pKa = 11.84VAALNVRR187 pKa = 11.84SAPRR191 pKa = 11.84LDD193 pKa = 3.6AEE195 pKa = 4.28IVATYY200 pKa = 9.87GEE202 pKa = 4.27NMEE205 pKa = 4.57FNYY208 pKa = 10.77DD209 pKa = 3.03GWIDD213 pKa = 3.51SDD215 pKa = 4.15GYY217 pKa = 10.69IWVTYY222 pKa = 10.35ISVTGVRR229 pKa = 11.84RR230 pKa = 11.84YY231 pKa = 9.74VAVGNSQNGRR241 pKa = 11.84RR242 pKa = 11.84VTNFGTFRR250 pKa = 4.37

Molecular weight: 27.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A0N1|A0A4D6A0N1_9CAUD Capsid protein OS=Streptococcus phage Javan161 OX=2548014 GN=Javan161_0024 PE=4 SV=1
MM1 pKa = 6.82TTDD4 pKa = 4.11EE5 pKa = 4.69LLQNLRR11 pKa = 11.84DD12 pKa = 4.25DD13 pKa = 4.16LNKK16 pKa = 10.21IINVLKK22 pKa = 10.59NCWKK26 pKa = 10.59ALLFLAIAILGMMVTVSYY44 pKa = 10.2FSYY47 pKa = 10.92RR48 pKa = 11.84DD49 pKa = 3.38AQQYY53 pKa = 9.11YY54 pKa = 8.76EE55 pKa = 4.01PQINGLRR62 pKa = 11.84AQLSRR67 pKa = 11.84TQKK70 pKa = 8.3QLKK73 pKa = 9.29RR74 pKa = 11.84ASEE77 pKa = 3.82DD78 pKa = 2.95RR79 pKa = 11.84ARR81 pKa = 11.84QTKK84 pKa = 9.88RR85 pKa = 11.84MAEE88 pKa = 3.91LTGNGGG94 pKa = 3.26

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12717

44

1307

205.1

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.36 ± 0.58

0.605 ± 0.1

6.778 ± 0.303

7.329 ± 0.251

3.617 ± 0.198

6.393 ± 0.297

1.392 ± 0.137

6.708 ± 0.312

8.768 ± 0.353

8.768 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.351 ± 0.156

5.402 ± 0.196

3.067 ± 0.262

4.349 ± 0.231

4.003 ± 0.18

5.756 ± 0.315

6.078 ± 0.219

6.283 ± 0.28

1.297 ± 0.129

3.696 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski