Klebsiella phage vB_KpnM_IME346

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; unclassified Jedunavirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DTA3|A0A4D6DTA3_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_IME346 OX=2562174 PE=4 SV=1
MM1 pKa = 7.53QDD3 pKa = 4.48DD4 pKa = 4.42DD5 pKa = 4.29EE6 pKa = 6.11QYY8 pKa = 11.45DD9 pKa = 3.97SPNGSEE15 pKa = 4.13WIVAVDD21 pKa = 2.85KK22 pKa = 10.7WGVVTILKK30 pKa = 8.55TPNIHH35 pKa = 6.94YY36 pKa = 10.72SLLDD40 pKa = 3.81PGNAEE45 pKa = 4.27EE46 pKa = 4.65IGLPFEE52 pKa = 4.47VEE54 pKa = 4.16DD55 pKa = 4.59YY56 pKa = 11.19IPGVYY61 pKa = 8.92KK62 pKa = 9.99WKK64 pKa = 10.72CNLTVGTDD72 pKa = 3.01WEE74 pKa = 4.35TGYY77 pKa = 11.03VDD79 pKa = 4.28EE80 pKa = 6.18IEE82 pKa = 4.46FEE84 pKa = 4.54VIEE87 pKa = 4.34EE88 pKa = 4.09TLLWSVEE95 pKa = 4.03QQ96 pKa = 3.8

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DRM5|A0A4D6DRM5_9CAUD N-acetyltransferase OS=Klebsiella phage vB_KpnM_IME346 OX=2562174 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 10.05IKK4 pKa = 10.46QLLRR8 pKa = 11.84PSYY11 pKa = 10.05GWYY14 pKa = 10.37KK15 pKa = 9.99STSQKK20 pKa = 11.17DD21 pKa = 2.96KK22 pKa = 10.87WFLDD26 pKa = 3.81DD27 pKa = 3.85VQIPMYY33 pKa = 9.93VARR36 pKa = 11.84KK37 pKa = 9.41LIRR40 pKa = 11.84KK41 pKa = 9.07ARR43 pKa = 11.84MVEE46 pKa = 4.2SNHH49 pKa = 4.56VRR51 pKa = 11.84KK52 pKa = 10.14VV53 pKa = 3.07

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14418

32

752

182.5

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.593 ± 0.436

1.526 ± 0.18

6.221 ± 0.199

5.771 ± 0.285

3.267 ± 0.191

7.733 ± 0.318

1.852 ± 0.17

5.847 ± 0.183

5.209 ± 0.316

7.463 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.132

4.945 ± 0.242

4.148 ± 0.197

4.05 ± 0.253

5.084 ± 0.243

6.242 ± 0.298

6.901 ± 0.321

7.262 ± 0.214

1.616 ± 0.097

3.447 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski