Mycobacterium phage DS6A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hnatkovirus; Mycobacterium virus DS6A

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8I4D6|G8I4D6_9CAUD Uncharacterized protein OS=Mycobacterium phage DS6A OX=45764 GN=26 PE=4 SV=1
MM1 pKa = 6.91ATIRR5 pKa = 11.84ATFTDD10 pKa = 3.9AEE12 pKa = 4.42GEE14 pKa = 4.12PAVGYY19 pKa = 8.81VQFEE23 pKa = 3.84PRR25 pKa = 11.84YY26 pKa = 9.95LRR28 pKa = 11.84NAVLAPTVRR37 pKa = 11.84DD38 pKa = 3.46GMVAPVRR45 pKa = 11.84LRR47 pKa = 11.84FPLDD51 pKa = 3.99DD52 pKa = 5.11GEE54 pKa = 5.27LEE56 pKa = 4.52ADD58 pKa = 5.0LEE60 pKa = 4.66PGDD63 pKa = 4.04YY64 pKa = 10.67NVAVVLQYY72 pKa = 11.48ARR74 pKa = 11.84TIYY77 pKa = 10.75AVATVPLGSGVINLRR92 pKa = 11.84DD93 pKa = 3.66LLSAYY98 pKa = 9.8MPVPQRR104 pKa = 11.84ATNEE108 pKa = 3.7YY109 pKa = 8.17STPGDD114 pKa = 3.79EE115 pKa = 4.89IEE117 pKa = 3.96WAIPFGATKK126 pKa = 9.83IDD128 pKa = 3.85VVLLGAGGGGDD139 pKa = 3.71PGDD142 pKa = 3.92LFVGGGGLCGAWLGITLEE160 pKa = 4.46RR161 pKa = 11.84GVDD164 pKa = 4.06FPGATQAVSGVIGVGGASGEE184 pKa = 4.43DD185 pKa = 3.86GADD188 pKa = 3.26TTLTVPGITNPAAVGGLLKK207 pKa = 10.56ADD209 pKa = 4.22GGLHH213 pKa = 6.01GANKK217 pKa = 8.49GTLLGTPDD225 pKa = 4.02QPISWGQAVAPFEE238 pKa = 4.57FNGRR242 pKa = 11.84TYY244 pKa = 10.89EE245 pKa = 4.29GGQIQTMWGGDD256 pKa = 3.35GFGPGGGGSGGFSYY270 pKa = 11.12SPGGAGAGGKK280 pKa = 8.64IWLTGYY286 pKa = 10.67

Molecular weight: 28.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8I4H0|G8I4H0_9CAUD Uncharacterized protein OS=Mycobacterium phage DS6A OX=45764 GN=60 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84SLAYY6 pKa = 8.95THH8 pKa = 6.53SAYY11 pKa = 10.18QAIAPGRR18 pKa = 11.84EE19 pKa = 3.91MATDD23 pKa = 4.2TPATRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LRR32 pKa = 11.84LLVDD36 pKa = 3.44GEE38 pKa = 4.06PARR41 pKa = 11.84WHH43 pKa = 7.19IDD45 pKa = 3.36PQPPDD50 pKa = 4.09PGFNWHH56 pKa = 5.33TVGVGRR62 pKa = 11.84ADD64 pKa = 3.16KK65 pKa = 10.48VSKK68 pKa = 10.52RR69 pKa = 11.84RR70 pKa = 11.84FNNWEE75 pKa = 3.94TLL77 pKa = 3.51

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

19046

27

1208

196.4

21.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.654 ± 0.5

0.914 ± 0.111

6.584 ± 0.225

5.529 ± 0.229

2.352 ± 0.168

9.976 ± 0.452

2.174 ± 0.156

3.591 ± 0.182

2.998 ± 0.229

8.054 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.084 ± 0.105

2.3 ± 0.167

5.613 ± 0.242

3.129 ± 0.152

8.275 ± 0.549

3.964 ± 0.213

5.902 ± 0.235

7.351 ± 0.319

2.352 ± 0.168

2.205 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski