Organic Lake virophage

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2Y0M5|F2Y0M5_9VIRU Uncharacterized protein OS=Organic Lake virophage OX=938080 GN=OLV21 PE=4 SV=1
MM1 pKa = 7.55FNLTDD6 pKa = 4.01DD7 pKa = 4.16SKK9 pKa = 11.15VTILKK14 pKa = 9.77SGNYY18 pKa = 8.81KK19 pKa = 10.18VEE21 pKa = 4.33FMCGFFNVSKK31 pKa = 10.51GRR33 pKa = 11.84ANIRR37 pKa = 11.84VGCRR41 pKa = 11.84INGIYY46 pKa = 10.52DD47 pKa = 3.41KK48 pKa = 10.85TFGGQPSCYY57 pKa = 9.88LRR59 pKa = 11.84DD60 pKa = 3.5QAYY63 pKa = 9.78VRR65 pKa = 11.84YY66 pKa = 9.13GSCSNSLYY74 pKa = 10.73FSLNANDD81 pKa = 4.34TIEE84 pKa = 5.06LEE86 pKa = 4.49SNLNLGDD93 pKa = 4.21EE94 pKa = 4.65IGFDD98 pKa = 4.2SNFDD102 pKa = 3.58SAFQLLRR109 pKa = 11.84GSNILITYY117 pKa = 9.56LDD119 pKa = 3.44QTGPQGDD126 pKa = 3.9TGATGADD133 pKa = 4.25GIDD136 pKa = 4.56GIDD139 pKa = 3.65GATGATGPQGIQGEE153 pKa = 4.54TGADD157 pKa = 3.53GADD160 pKa = 3.73GADD163 pKa = 3.85GADD166 pKa = 3.9GATGPTGPTGVDD178 pKa = 3.29GADD181 pKa = 3.66GATGPQGIQGEE192 pKa = 4.49TGPQGIQGEE201 pKa = 4.54TGADD205 pKa = 3.59GADD208 pKa = 3.71GAIGPTGPAGADD220 pKa = 3.56GEE222 pKa = 4.69VTLAQLNTKK231 pKa = 10.11QDD233 pKa = 3.29VLTAGNNITIDD244 pKa = 3.7VNNVISSSGGEE255 pKa = 4.01GGGGITQQEE264 pKa = 4.4LDD266 pKa = 4.66DD267 pKa = 4.44GLEE270 pKa = 4.11TKK272 pKa = 10.36QDD274 pKa = 3.3ILTAGANISIVGSTINSGSSAYY296 pKa = 9.76FLCFLNLNYY305 pKa = 10.39TNLILGDD312 pKa = 3.72YY313 pKa = 10.65ARR315 pKa = 11.84FPAISFQKK323 pKa = 10.16PNTGTMVEE331 pKa = 3.88QHH333 pKa = 6.65RR334 pKa = 11.84GHH336 pKa = 7.48AYY338 pKa = 8.99TIQEE342 pKa = 4.06TGLYY346 pKa = 9.92LIGYY350 pKa = 8.15SLTMLNQGGTTAIQIVYY367 pKa = 9.23VRR369 pKa = 11.84NGVEE373 pKa = 4.89KK374 pKa = 10.65SITYY378 pKa = 10.38NGITIPTSEE387 pKa = 4.16NRR389 pKa = 11.84SIIFPLEE396 pKa = 4.21AGDD399 pKa = 5.14LIAVKK404 pKa = 10.25YY405 pKa = 10.49KK406 pKa = 10.59SGNAGYY412 pKa = 8.1LTLYY416 pKa = 7.5GTPSTEE422 pKa = 4.06NIQTNMYY429 pKa = 9.68GYY431 pKa = 10.62RR432 pKa = 11.84IAA434 pKa = 5.59

Molecular weight: 45.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2Y0M1|F2Y0M1_9VIRU Uncharacterized protein OS=Organic Lake virophage OX=938080 GN=OLV16 PE=4 SV=1
MM1 pKa = 7.3RR2 pKa = 11.84TSSTLRR8 pKa = 11.84TSSTSSTNSTISPINSISPSRR29 pKa = 11.84TLRR32 pKa = 11.84TSSTSLSLRR41 pKa = 11.84TLWTSSTINAISTSSTSSTINTINTSRR68 pKa = 11.84TSFALYY74 pKa = 9.08TLWTSSTINTINTSRR89 pKa = 11.84TSFALYY95 pKa = 9.08TLWTSSTINTISTLWTSSTSSTSSTINTVNSISTSFALYY134 pKa = 9.1TLRR137 pKa = 11.84TSFALYY143 pKa = 9.08TLWTSSTSSTSSTINTVNSISTSFALYY170 pKa = 9.1TLRR173 pKa = 11.84TSFALYY179 pKa = 9.08TLWTSSTSSTSSTSSTSSTNSTVSTVNSISTSSTSRR215 pKa = 11.84TLRR218 pKa = 11.84TSSTSSTNSTSSTNSTVNSISSISTSRR245 pKa = 11.84TSSTISSISSISSISSSRR263 pKa = 11.84TSRR266 pKa = 11.84TSFALYY272 pKa = 9.08TLWTSSTINPISTISTISTSFALYY296 pKa = 9.1TLRR299 pKa = 11.84TSFALYY305 pKa = 9.13TLRR308 pKa = 11.84TSSTISPISPISTISPISTISTSRR332 pKa = 11.84TSRR335 pKa = 11.84TSFALYY341 pKa = 9.08TLWTSFSYY349 pKa = 11.3VPFFSCRR356 pKa = 11.84RR357 pKa = 11.84NYY359 pKa = 9.77ISCLINRR366 pKa = 11.84YY367 pKa = 9.46LCTGYY372 pKa = 11.21

Molecular weight: 39.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

8420

96

1036

350.8

38.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.903 ± 0.671

1.247 ± 0.303

5.95 ± 0.545

5.523 ± 0.507

3.349 ± 0.382

7.518 ± 1.449

1.366 ± 0.224

7.031 ± 0.359

6.722 ± 1.095

8.29 ± 0.672

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.276

6.354 ± 0.465

4.537 ± 1.028

3.99 ± 0.497

2.922 ± 0.258

9.204 ± 1.144

8.029 ± 0.805

5.475 ± 0.562

0.665 ± 0.135

3.86 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski