Bacteriovorax sp. BSW11_IV
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|T0RE05|T0RE05_9PROT Peptidase MA family protein OS=Bacteriovorax sp. BSW11_IV OX=1353529 GN=M899_0510 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.56 KK3 pKa = 10.27 LMAFIFLSLLFSSCKK18 pKa = 9.68 NQMNVEE24 pKa = 4.15 LNDD27 pKa = 4.36 LIKK30 pKa = 11.18 ANASYY35 pKa = 11.29 GDD37 pKa = 4.17 EE38 pKa = 4.19 SSSDD42 pKa = 3.5 NPVVEE47 pKa = 4.68 TYY49 pKa = 10.6 GYY51 pKa = 10.83 RR52 pKa = 11.84 LDD54 pKa = 4.02 ATDD57 pKa = 4.47 EE58 pKa = 4.32 EE59 pKa = 5.34 YY60 pKa = 10.12 ATYY63 pKa = 10.44 SGYY66 pKa = 11.16 ALQMDD71 pKa = 4.77 STYY74 pKa = 11.28 LAATYY79 pKa = 10.63 TMTGAQKK86 pKa = 10.36 FNYY89 pKa = 9.34 GIPTNFSGTDD99 pKa = 3.39 NQKK102 pKa = 10.9 LDD104 pKa = 3.51 FTSGVKK110 pKa = 10.45 AFEE113 pKa = 4.38 CDD115 pKa = 2.67 ISYY118 pKa = 10.69 IDD120 pKa = 4.52 AAPTDD125 pKa = 4.23 SIFGQICGVSEE136 pKa = 4.98 FITNKK141 pKa = 10.03 GVLFQIVVNNSNITVIIVIDD161 pKa = 3.58 NAIVYY166 pKa = 8.09 SQSLTSLDD174 pKa = 3.8 EE175 pKa = 4.26 APEE178 pKa = 4.5 RR179 pKa = 11.84 IGFEE183 pKa = 4.36 LNANTGIVTIYY194 pKa = 9.88 FDD196 pKa = 4.3 NIIVPLSSNAYY207 pKa = 8.19 TVMEE211 pKa = 4.13 YY212 pKa = 10.84 LGVLSIQEE220 pKa = 4.08 GDD222 pKa = 3.44 GNTNNTGKK230 pKa = 8.1 EE231 pKa = 3.71 TSMRR235 pKa = 11.84 LVLDD239 pKa = 4.23 GSDD242 pKa = 3.18 MTNAYY247 pKa = 7.63 DD248 pKa = 4.56 TPTTDD253 pKa = 3.15 PFGGPVANIVFF264 pKa = 4.14
Molecular weight: 28.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.859
IPC_protein 3.846
Toseland 3.643
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.681
Grimsley 3.541
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.694
EMBOSS 3.783
Sillero 3.973
Patrickios 0.413
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|T0RJ87|T0RJ87_9PROT 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Bacteriovorax sp. BSW11_IV OX=1353529 GN=fabF PE=3 SV=1
MM1 pKa = 7.21 AAKK4 pKa = 8.8 RR5 pKa = 11.84 TWQPKK10 pKa = 7.59 KK11 pKa = 9.93 KK12 pKa = 9.89 KK13 pKa = 9.33 RR14 pKa = 11.84 LRR16 pKa = 11.84 VHH18 pKa = 6.74 GFLKK22 pKa = 10.62 RR23 pKa = 11.84 MSTAGGKK30 pKa = 9.57 KK31 pKa = 9.85 VISARR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 10.05 GRR41 pKa = 11.84 HH42 pKa = 4.95 SLTVKK47 pKa = 9.93 TGTKK51 pKa = 9.97
Molecular weight: 5.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3457
0
3457
1125166
37
4321
325.5
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.375 ± 0.045
1.062 ± 0.015
5.68 ± 0.03
7.048 ± 0.059
5.24 ± 0.041
6.302 ± 0.054
1.944 ± 0.021
7.295 ± 0.037
8.11 ± 0.056
9.657 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.393 ± 0.021
5.413 ± 0.041
3.214 ± 0.026
3.086 ± 0.022
3.835 ± 0.026
7.297 ± 0.051
5.249 ± 0.053
6.308 ± 0.035
0.881 ± 0.013
3.611 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here