Manihot esculenta (Cassava) (Jatropha manihot)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C9UEG1|A0A2C9UEG1_MANES Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_15G063900 PE=4 SV=1
MM1 pKa = 7.51SNRR4 pKa = 11.84IVMEE8 pKa = 4.03KK9 pKa = 10.06KK10 pKa = 9.79IKK12 pKa = 10.73NGVVDD17 pKa = 3.86VSSFLLVEE25 pKa = 4.7GSADD29 pKa = 3.69SEE31 pKa = 4.44ADD33 pKa = 3.37CGLLKK38 pKa = 10.72LCEE41 pKa = 4.18DD42 pKa = 4.36VIMACDD48 pKa = 3.84YY49 pKa = 11.46DD50 pKa = 5.76DD51 pKa = 5.54EE52 pKa = 5.24DD53 pKa = 6.29AEE55 pKa = 4.52SCSCDD60 pKa = 3.19TVDD63 pKa = 3.52SLEE66 pKa = 4.07VFDD69 pKa = 5.13YY70 pKa = 11.2DD71 pKa = 4.76GTGGDD76 pKa = 4.26QDD78 pKa = 3.66CSGYY82 pKa = 11.11DD83 pKa = 3.14EE84 pKa = 5.55AKK86 pKa = 10.72ANKK89 pKa = 9.2EE90 pKa = 3.92CAEE93 pKa = 3.85EE94 pKa = 4.51RR95 pKa = 11.84NWCRR99 pKa = 11.84LWLGVAGLEE108 pKa = 4.52YY109 pKa = 10.12MSTEE113 pKa = 3.94NGEE116 pKa = 4.41EE117 pKa = 4.11EE118 pKa = 4.51SKK120 pKa = 11.52ADD122 pKa = 3.69AKK124 pKa = 10.07WSKK127 pKa = 10.47EE128 pKa = 4.09VIDD131 pKa = 4.09QMEE134 pKa = 4.73DD135 pKa = 3.29SLFWEE140 pKa = 4.34TCLAVGYY147 pKa = 8.89PP148 pKa = 3.6

Molecular weight: 16.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C9U9V0|A0A2C9U9V0_MANES Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_16G023000 PE=4 SV=1
MM1 pKa = 7.51AGARR5 pKa = 11.84AAMINGRR12 pKa = 11.84KK13 pKa = 9.13ISRR16 pKa = 11.84SSSGRR21 pKa = 11.84PIPKK25 pKa = 9.52RR26 pKa = 11.84GQVKK30 pKa = 8.93VAIVVGLAHH39 pKa = 6.42SVASIFSLNSRR50 pKa = 11.84RR51 pKa = 11.84AGPASLFSS59 pKa = 3.71

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32935

4798

37733

15226168

11

5344

403.5

45.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.642 ± 0.013

1.906 ± 0.007

5.231 ± 0.009

6.607 ± 0.029

4.262 ± 0.01

6.403 ± 0.012

2.41 ± 0.006

5.52 ± 0.011

6.196 ± 0.014

9.819 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.423 ± 0.005

4.609 ± 0.01

4.806 ± 0.011

3.769 ± 0.009

5.132 ± 0.01

9.106 ± 0.015

4.777 ± 0.008

6.339 ± 0.008

1.262 ± 0.005

2.78 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski