Torque teno mini virus 4
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9DUB2|Q9DUB2_9VIRU Uncharacterized protein OS=Torque teno mini virus 4 OX=687372 PE=4 SV=1
MM1 pKa = 7.56 SGNFKK6 pKa = 9.36 PTFYY10 pKa = 10.38 TPKK13 pKa = 10.42 SKK15 pKa = 10.62 GKK17 pKa = 10.27 ALLNSVAHH25 pKa = 6.21 SHH27 pKa = 7.55 DD28 pKa = 4.27 LLCHH32 pKa = 6.5 CDD34 pKa = 3.56 HH35 pKa = 7.23 PLKK38 pKa = 10.41 HH39 pKa = 6.14 LCEE42 pKa = 5.46 IIFEE46 pKa = 4.48 NEE48 pKa = 3.93 PEE50 pKa = 4.57 LKK52 pKa = 10.59 LQLCHH57 pKa = 5.76 TTTAEE62 pKa = 4.15 TGDD65 pKa = 3.57 SHH67 pKa = 6.73 TAGDD71 pKa = 4.04 DD72 pKa = 3.8 GFGEE76 pKa = 4.62 GDD78 pKa = 3.9 LDD80 pKa = 4.16 RR81 pKa = 11.84 LFAEE85 pKa = 5.23 DD86 pKa = 3.87 FTEE89 pKa = 4.35 EE90 pKa = 4.15 GG91 pKa = 3.57
Molecular weight: 10.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.743
IPC2_protein 4.838
IPC_protein 4.749
Toseland 4.698
ProMoST 4.902
Dawson 4.749
Bjellqvist 4.876
Wikipedia 4.635
Rodwell 4.66
Grimsley 4.622
Solomon 4.749
Lehninger 4.711
Nozaki 4.876
DTASelect 5.029
Thurlkill 4.698
EMBOSS 4.673
Sillero 4.94
Patrickios 3.503
IPC_peptide 4.749
IPC2_peptide 4.94
IPC2.peptide.svr19 4.824
Protein with the highest isoelectric point:
>tr|Q9DUB2|Q9DUB2_9VIRU Uncharacterized protein OS=Torque teno mini virus 4 OX=687372 PE=4 SV=1
MM1 pKa = 7.71 PYY3 pKa = 10.16 YY4 pKa = 10.58 YY5 pKa = 10.07 RR6 pKa = 11.84 RR7 pKa = 11.84 NWRR10 pKa = 11.84 LPYY13 pKa = 9.86 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 WFWRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 PRR25 pKa = 11.84 PFIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 FHH33 pKa = 6.09 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 VRR38 pKa = 11.84 RR39 pKa = 11.84 QPYY42 pKa = 8.21 FHH44 pKa = 7.42 KK45 pKa = 10.52 KK46 pKa = 9.38 LKK48 pKa = 10.01 KK49 pKa = 10.39 LKK51 pKa = 9.47 LQQYY55 pKa = 6.83 QPRR58 pKa = 11.84 TVRR61 pKa = 11.84 YY62 pKa = 8.74 CKK64 pKa = 10.22 IKK66 pKa = 10.58 GFQCLFEE73 pKa = 4.45 ASKK76 pKa = 11.15 YY77 pKa = 10.53 RR78 pKa = 11.84 IGNNYY83 pKa = 7.92 TMYY86 pKa = 10.56 FNTFAPEE93 pKa = 3.91 HH94 pKa = 6.32 LPGGGGFSIVKK105 pKa = 10.07 YY106 pKa = 10.09 SLEE109 pKa = 4.0 SLYY112 pKa = 10.95 SEE114 pKa = 4.54 HH115 pKa = 7.37 EE116 pKa = 4.23 YY117 pKa = 9.85 VRR119 pKa = 11.84 NWWTKK124 pKa = 10.46 SNLNLPLFRR133 pKa = 11.84 YY134 pKa = 9.48 LGCTLKK140 pKa = 10.69 LYY142 pKa = 10.43 QSEE145 pKa = 4.24 DD146 pKa = 2.63 VDD148 pKa = 4.72 YY149 pKa = 11.4 AFQYY153 pKa = 8.59 QNCYY157 pKa = 10.18 PMVATAHH164 pKa = 6.97 LYY166 pKa = 10.04 ISRR169 pKa = 11.84 QPSIMMMNNHH179 pKa = 6.78 SILVPSKK186 pKa = 9.41 RR187 pKa = 11.84 TQKK190 pKa = 10.32 RR191 pKa = 11.84 KK192 pKa = 10.13 KK193 pKa = 8.3 PCIKK197 pKa = 10.13 IKK199 pKa = 10.55 VSPPSQMEE207 pKa = 4.22 NKK209 pKa = 9.39 WYY211 pKa = 9.45 FQKK214 pKa = 10.98 DD215 pKa = 3.28 NCKK218 pKa = 9.14 TPLLITTATACSFDD232 pKa = 3.65 HH233 pKa = 6.91 YY234 pKa = 11.37 YY235 pKa = 11.47 LNTSAKK241 pKa = 9.19 NNCITIYY248 pKa = 10.88 SLNTYY253 pKa = 9.68 LYY255 pKa = 10.27 QSRR258 pKa = 11.84 HH259 pKa = 4.16 FNNRR263 pKa = 11.84 GTTGYY268 pKa = 10.04 HH269 pKa = 6.54 PKK271 pKa = 10.75 NNTYY275 pKa = 10.74 LYY277 pKa = 8.3 GTLEE281 pKa = 4.36 NGPKK285 pKa = 10.38 YY286 pKa = 10.47 KK287 pKa = 10.79 VGDD290 pKa = 4.66 LIYY293 pKa = 10.62 LGNTMTYY300 pKa = 9.71 QQGQSPRR307 pKa = 11.84 EE308 pKa = 3.95 LHH310 pKa = 6.25 PTDD313 pKa = 3.3 TTKK316 pKa = 10.92 GFQEE320 pKa = 4.21 AKK322 pKa = 10.46 QFEE325 pKa = 4.52 NWGNPFYY332 pKa = 10.54 THH334 pKa = 6.66 NLHH337 pKa = 5.68 GTIHH341 pKa = 7.01 ILQTTEE347 pKa = 4.93 SPTQMYY353 pKa = 9.08 TGTSYY358 pKa = 10.67 EE359 pKa = 4.03 AEE361 pKa = 4.17 KK362 pKa = 10.72 TNLTEE367 pKa = 4.13 ISNNFYY373 pKa = 8.73 TLRR376 pKa = 11.84 YY377 pKa = 9.02 NPYY380 pKa = 9.15 RR381 pKa = 11.84 DD382 pKa = 3.38 SGEE385 pKa = 3.94 GNKK388 pKa = 9.62 IYY390 pKa = 10.64 LLKK393 pKa = 10.8 NWTEE397 pKa = 3.99 EE398 pKa = 4.35 TGWDD402 pKa = 3.97 PPTDD406 pKa = 3.23 TDD408 pKa = 6.39 LIMEE412 pKa = 5.71 GYY414 pKa = 9.35 PLWLMLWGWIDD425 pKa = 3.45 WQKK428 pKa = 11.66 KK429 pKa = 6.56 LAKK432 pKa = 10.08 INTIDD437 pKa = 3.09 TKK439 pKa = 10.99 YY440 pKa = 10.81 ILVIQTKK447 pKa = 6.34 FTKK450 pKa = 10.69 GEE452 pKa = 3.85 MPIIVPIDD460 pKa = 3.41 YY461 pKa = 10.89 DD462 pKa = 4.3 FIDD465 pKa = 4.36 GQSPYY470 pKa = 10.28 TEE472 pKa = 4.45 EE473 pKa = 5.5 LDD475 pKa = 4.18 PTDD478 pKa = 3.64 KK479 pKa = 11.25 QNWHH483 pKa = 6.99 PMVQYY488 pKa = 9.79 QQQTINNILTCGPGTPKK505 pKa = 10.81 YY506 pKa = 9.81 NGKK509 pKa = 8.51 NTVEE513 pKa = 4.21 AKK515 pKa = 10.61 LEE517 pKa = 3.86 YY518 pKa = 10.41 SFKK521 pKa = 10.74 FKK523 pKa = 10.83 FGGCPPPMAALEE535 pKa = 4.62 DD536 pKa = 4.04 PCKK539 pKa = 10.53 QPTYY543 pKa = 10.19 QIPQTTTMLQDD554 pKa = 3.25 PTTPLQYY561 pKa = 10.97 YY562 pKa = 9.42 LWNFDD567 pKa = 3.33 EE568 pKa = 6.29 RR569 pKa = 11.84 RR570 pKa = 11.84 GQLTKK575 pKa = 10.1 TAADD579 pKa = 5.33 RR580 pKa = 11.84 IQKK583 pKa = 9.61 DD584 pKa = 3.17 WQLTDD589 pKa = 3.85 SVISTTGLHH598 pKa = 6.83 AKK600 pKa = 10.08 DD601 pKa = 4.33 LEE603 pKa = 4.26 PLQTHH608 pKa = 6.08 QEE610 pKa = 4.12 IQDD613 pKa = 3.54 QTSSEE618 pKa = 4.28 EE619 pKa = 3.96 EE620 pKa = 3.98 SEE622 pKa = 3.99 EE623 pKa = 5.39 DD624 pKa = 4.62 IFSQLQRR631 pKa = 11.84 QRR633 pKa = 11.84 AKK635 pKa = 7.71 QHH637 pKa = 6.99 RR638 pKa = 11.84 IRR640 pKa = 11.84 QRR642 pKa = 11.84 ILQLVTKK649 pKa = 9.71 IQKK652 pKa = 10.4 LEE654 pKa = 3.84
Molecular weight: 77.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.768
IPC2_protein 8.902
IPC_protein 8.785
Toseland 9.209
ProMoST 9.165
Dawson 9.589
Bjellqvist 9.399
Wikipedia 9.809
Rodwell 9.721
Grimsley 9.692
Solomon 9.604
Lehninger 9.545
Nozaki 9.385
DTASelect 9.341
Thurlkill 9.399
EMBOSS 9.677
Sillero 9.545
Patrickios 4.19
IPC_peptide 9.589
IPC2_peptide 8.126
IPC2.peptide.svr19 7.822
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
745
91
654
372.5
43.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.087 ± 1.009
2.013 ± 0.998
4.564 ± 1.772
5.772 ± 1.726
4.161 ± 1.019
5.369 ± 1.434
3.221 ± 1.874
5.235 ± 1.273
7.248 ± 0.274
8.725 ± 1.409
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.148 ± 0.44
5.503 ± 0.925
6.174 ± 0.746
6.443 ± 2.24
5.503 ± 1.846
4.698 ± 0.334
9.262 ± 0.197
2.148 ± 0.44
2.148 ± 0.9
6.577 ± 2.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here