Haemophilus phage SuMu
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F6MIJ4|F6MIJ4_9CAUD Uncharacterized protein OS=Haemophilus phage SuMu OX=483266 GN=SuMu_12 PE=4 SV=1
MM1 pKa = 7.67 EE2 pKa = 4.11 KK3 pKa = 10.03 TYY5 pKa = 10.31 FYY7 pKa = 10.55 DD8 pKa = 3.08 YY9 pKa = 10.25 WYY11 pKa = 11.1 NKK13 pKa = 10.29 DD14 pKa = 4.79 DD15 pKa = 4.87 IDD17 pKa = 5.7 YY18 pKa = 11.32 DD19 pKa = 4.04 DD20 pKa = 5.78 LKK22 pKa = 11.35 HH23 pKa = 6.93 KK24 pKa = 8.35 NTKK27 pKa = 9.91 QFTADD32 pKa = 3.14 IEE34 pKa = 4.32 KK35 pKa = 10.99 EE36 pKa = 4.14 EE37 pKa = 4.22 LSAMAEE43 pKa = 4.43 GISAIIIRR51 pKa = 11.84 EE52 pKa = 3.81 LRR54 pKa = 11.84 GNNWGEE60 pKa = 3.87 FGEE63 pKa = 4.54 GDD65 pKa = 3.59 EE66 pKa = 5.36 LYY68 pKa = 10.33 IAFKK72 pKa = 10.42 EE73 pKa = 4.1 QGADD77 pKa = 3.06 GVMLYY82 pKa = 10.19 KK83 pKa = 10.09 IKK85 pKa = 10.69 EE86 pKa = 4.25 VMVPSYY92 pKa = 10.75 VAEE95 pKa = 4.05 EE96 pKa = 3.91 LVGEE100 pKa = 4.12 NDD102 pKa = 3.28 EE103 pKa = 4.29
Molecular weight: 12.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.276
IPC2_protein 4.24
IPC_protein 4.164
Toseland 3.986
ProMoST 4.24
Dawson 4.113
Bjellqvist 4.304
Wikipedia 3.986
Rodwell 3.999
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.228
DTASelect 4.368
Thurlkill 4.012
EMBOSS 3.999
Sillero 4.266
Patrickios 3.465
IPC_peptide 4.113
IPC2_peptide 4.253
IPC2.peptide.svr19 4.202
Protein with the highest isoelectric point:
>tr|F6MII9|F6MII9_9CAUD Uncharacterized protein OS=Haemophilus phage SuMu OX=483266 GN=SuMu_7 PE=4 SV=1
MM1 pKa = 8.47 RR2 pKa = 11.84 YY3 pKa = 8.65 RR4 pKa = 11.84 QIYY7 pKa = 7.78 AAYY10 pKa = 9.71 RR11 pKa = 11.84 GEE13 pKa = 4.16 EE14 pKa = 3.99 NVADD18 pKa = 4.14 GTAEE22 pKa = 3.78 EE23 pKa = 4.4 LAIKK27 pKa = 10.54 LGVTAKK33 pKa = 9.13 TVRR36 pKa = 11.84 KK37 pKa = 8.61 WSTPSYY43 pKa = 9.74 HH44 pKa = 6.75 RR45 pKa = 11.84 RR46 pKa = 11.84 NKK48 pKa = 7.47 GQRR51 pKa = 11.84 VIVVKK56 pKa = 10.72 LGKK59 pKa = 10.22 EE60 pKa = 3.97 KK61 pKa = 11.0 VVV63 pKa = 3.28
Molecular weight: 7.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.247
IPC2_protein 9.721
IPC_protein 10.072
Toseland 10.423
ProMoST 10.058
Dawson 10.57
Bjellqvist 10.218
Wikipedia 10.73
Rodwell 11.008
Grimsley 10.628
Solomon 10.613
Lehninger 10.584
Nozaki 10.379
DTASelect 10.218
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.482
Patrickios 10.76
IPC_peptide 10.613
IPC2_peptide 8.843
IPC2.peptide.svr19 8.728
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
55
0
55
11407
37
787
207.4
23.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.986 ± 0.469
1.087 ± 0.15
5.497 ± 0.266
7.154 ± 0.255
3.796 ± 0.192
6.531 ± 0.254
1.727 ± 0.157
5.795 ± 0.221
7.259 ± 0.307
9.249 ± 0.265
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.332 ± 0.142
4.822 ± 0.286
3.209 ± 0.195
5.023 ± 0.184
5.453 ± 0.238
5.882 ± 0.177
4.988 ± 0.272
6.768 ± 0.321
1.438 ± 0.156
3.007 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here