Sewage-associated circular DNA virus-22

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGM3|A0A0B4UGM3_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-22 OX=1592089 PE=4 SV=1
MM1 pKa = 7.55TSRR4 pKa = 11.84RR5 pKa = 11.84WCFTQNNPTEE15 pKa = 4.38DD16 pKa = 3.96EE17 pKa = 4.25QVHH20 pKa = 5.95LLWEE24 pKa = 4.63TWPHH28 pKa = 5.61LRR30 pKa = 11.84GGVCQLEE37 pKa = 4.37QGDD40 pKa = 4.31NGTPHH45 pKa = 5.34YY46 pKa = 10.01QGYY49 pKa = 10.23ISLDD53 pKa = 3.24RR54 pKa = 11.84PTRR57 pKa = 11.84LAALKK62 pKa = 10.31KK63 pKa = 10.06LLPRR67 pKa = 11.84AHH69 pKa = 6.66WEE71 pKa = 4.03VARR74 pKa = 11.84GDD76 pKa = 3.33ATANIVYY83 pKa = 7.69CTKK86 pKa = 10.67EE87 pKa = 3.79EE88 pKa = 3.97GRR90 pKa = 11.84IDD92 pKa = 3.87GPWRR96 pKa = 11.84IGEE99 pKa = 4.22IVDD102 pKa = 3.34QGHH105 pKa = 5.45RR106 pKa = 11.84TDD108 pKa = 3.39WDD110 pKa = 3.82KK111 pKa = 11.59LHH113 pKa = 6.99QDD115 pKa = 3.82IKK117 pKa = 11.3DD118 pKa = 3.55QLSDD122 pKa = 3.5QDD124 pKa = 3.31IADD127 pKa = 3.88RR128 pKa = 11.84NFRR131 pKa = 11.84LWIQHH136 pKa = 6.2RR137 pKa = 11.84SGIDD141 pKa = 2.96AYY143 pKa = 10.55RR144 pKa = 11.84ALHH147 pKa = 5.91SRR149 pKa = 11.84VRR151 pKa = 11.84DD152 pKa = 3.4EE153 pKa = 3.89KK154 pKa = 9.88TEE156 pKa = 3.95VVVICGPPGTGKK168 pKa = 10.61SSTARR173 pKa = 11.84EE174 pKa = 4.28LAPDD178 pKa = 4.53AYY180 pKa = 9.3WFPGGKK186 pKa = 7.65WWNDD190 pKa = 3.09YY191 pKa = 10.38NGQRR195 pKa = 11.84VVVIDD200 pKa = 4.01EE201 pKa = 4.38FVGTIQFTLLNRR213 pKa = 11.84ILDD216 pKa = 4.31RR217 pKa = 11.84YY218 pKa = 8.48PLQVEE223 pKa = 4.68TKK225 pKa = 9.51GGMRR229 pKa = 11.84NFTSKK234 pKa = 11.14LIFITSNKK242 pKa = 9.28KK243 pKa = 8.83PYY245 pKa = 9.44EE246 pKa = 3.89WYY248 pKa = 10.3GPEE251 pKa = 3.91NEE253 pKa = 4.57RR254 pKa = 11.84GALYY258 pKa = 10.39RR259 pKa = 11.84RR260 pKa = 11.84IDD262 pKa = 2.88WYY264 pKa = 9.93MWLDD268 pKa = 3.38RR269 pKa = 11.84LEE271 pKa = 4.7NFCIWKK277 pKa = 9.94KK278 pKa = 11.0GYY280 pKa = 10.58NEE282 pKa = 5.09DD283 pKa = 3.79YY284 pKa = 11.32

Molecular weight: 33.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGM3|A0A0B4UGM3_9VIRU Putative capsid protein OS=Sewage-associated circular DNA virus-22 OX=1592089 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84DD3 pKa = 3.31LLLFILLAGGFSMPYY18 pKa = 8.79IVVVLFSAVLFFVVVLVVVLSAIVSKK44 pKa = 10.17MALSNHH50 pKa = 5.38IAIARR55 pKa = 11.84SHH57 pKa = 6.65TGQAHH62 pKa = 6.86AGQVAYY68 pKa = 8.69ATEE71 pKa = 4.39FTNFPTAPTNTSFLDD86 pKa = 3.32SRR88 pKa = 11.84GFLLGQVRR96 pKa = 11.84GVGLQAKK103 pKa = 7.37MFCSGLASPYY113 pKa = 9.87FGEE116 pKa = 4.56IFADD120 pKa = 3.73ANPGVAAQDD129 pKa = 4.14NITSANVIHH138 pKa = 7.21DD139 pKa = 4.05PSVYY143 pKa = 10.35ACRR146 pKa = 11.84PCRR149 pKa = 11.84MRR151 pKa = 11.84FDD153 pKa = 5.11LSNQNVAPCDD163 pKa = 3.54VRR165 pKa = 11.84ITFLRR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84MPFNATSIIAGDD184 pKa = 3.99GSSTAWAEE192 pKa = 4.11GGQNDD197 pKa = 5.14PISTASTLFAQFQPEE212 pKa = 4.21AQSGALAANAVAPAFPTGRR231 pKa = 11.84WKK233 pKa = 10.57SAPGFTNYY241 pKa = 7.31WHH243 pKa = 6.9ILGFRR248 pKa = 11.84EE249 pKa = 4.52VRR251 pKa = 11.84NLAHH255 pKa = 6.9GAASTVYY262 pKa = 10.06LRR264 pKa = 11.84VPGSVFCPARR274 pKa = 11.84DD275 pKa = 3.59MFSVQPSVAGTYY287 pKa = 10.1EE288 pKa = 4.36YY289 pKa = 9.78VTPGYY294 pKa = 10.8DD295 pKa = 2.59VFYY298 pKa = 10.91VVEE301 pKa = 4.79VYY303 pKa = 10.47PKK305 pKa = 10.58APVIVSTSTTAGIVGRR321 pKa = 11.84STASINVCTAWTEE334 pKa = 3.68HH335 pKa = 4.02WTKK338 pKa = 10.35YY339 pKa = 8.48AQRR342 pKa = 11.84SRR344 pKa = 11.84WIASTYY350 pKa = 9.05TLSTTAVAGQNYY362 pKa = 8.22VAGRR366 pKa = 11.84EE367 pKa = 4.03IYY369 pKa = 9.61MNQYY373 pKa = 10.02GNAAGITSGPVQQ385 pKa = 3.34

Molecular weight: 41.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

669

284

385

334.5

37.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.417 ± 3.061

1.644 ± 0.074

4.933 ± 1.78

4.036 ± 1.456

4.634 ± 1.149

7.922 ± 0.112

2.242 ± 0.364

5.381 ± 0.382

2.84 ± 1.322

6.726 ± 0.645

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.644 ± 0.372

4.484 ± 0.059

5.082 ± 0.319

4.185 ± 0.248

6.726 ± 1.313

6.129 ± 1.872

6.876 ± 0.563

7.773 ± 1.798

2.99 ± 1.227

4.335 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski