Salmonella phage SP069
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4TVY0|S4TVY0_9CAUD Uncharacterized protein OS=Salmonella phage SP069 OX=1173760 GN=SP069_00080 PE=4 SV=1
MM1 pKa = 7.3 AQVKK5 pKa = 9.7 CIKK8 pKa = 10.51 DD9 pKa = 3.44 NEE11 pKa = 4.88 GYY13 pKa = 6.92 WTEE16 pKa = 4.19 GEE18 pKa = 4.5 VYY20 pKa = 9.9 HH21 pKa = 7.18 AEE23 pKa = 4.1 SAPGGFLNVGDD34 pKa = 5.57 DD35 pKa = 3.86 DD36 pKa = 5.09 DD37 pKa = 6.44 KK38 pKa = 11.25 NAEE41 pKa = 3.9 WSLMPVSYY49 pKa = 10.91 DD50 pKa = 3.88 DD51 pKa = 3.76 NDD53 pKa = 3.49 KK54 pKa = 11.22 AIYY57 pKa = 9.88 QLTGLDD63 pKa = 3.35 AQFIDD68 pKa = 3.84 VV69 pKa = 4.08
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.605
EMBOSS 3.719
Sillero 3.872
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.767
Protein with the highest isoelectric point:
>tr|S4TU54|S4TU54_9CAUD Uncharacterized protein OS=Salmonella phage SP069 OX=1173760 GN=SP069_00045 PE=4 SV=1
MM1 pKa = 7.47 IKK3 pKa = 10.45 VGLSPGASYY12 pKa = 10.78 AKK14 pKa = 10.48 QMIEE18 pKa = 3.77 QLKK21 pKa = 10.45 SLGEE25 pKa = 3.9 LTVEE29 pKa = 4.91 DD30 pKa = 4.44 FALFDD35 pKa = 5.41 GIPQVTQQLPTKK47 pKa = 9.32 QPRR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 8.44 TGVAQARR59 pKa = 11.84 HH60 pKa = 5.15 NARR63 pKa = 11.84 KK64 pKa = 9.55 RR65 pKa = 11.84 RR66 pKa = 11.84 NRR68 pKa = 11.84 KK69 pKa = 8.72
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.897
IPC_protein 10.833
Toseland 11.228
ProMoST 11.228
Dawson 11.272
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.506
Grimsley 11.286
Solomon 11.52
Lehninger 11.462
Nozaki 11.213
DTASelect 11.038
Thurlkill 11.213
EMBOSS 11.652
Sillero 11.213
Patrickios 11.257
IPC_peptide 11.52
IPC2_peptide 10.014
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
12341
58
971
192.8
21.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.225 ± 0.687
1.045 ± 0.158
6.418 ± 0.241
6.061 ± 0.303
3.476 ± 0.198
7.26 ± 0.438
1.783 ± 0.291
6.004 ± 0.275
5.543 ± 0.307
7.828 ± 0.264
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.617 ± 0.213
5.332 ± 0.283
4.1 ± 0.272
4.457 ± 0.665
4.959 ± 0.282
5.826 ± 0.271
6.685 ± 0.387
7.131 ± 0.306
1.58 ± 0.167
3.671 ± 0.268
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here