Vagococcus vulneris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429ZWQ2|A0A429ZWQ2_9ENTE Uncharacterized protein OS=Vagococcus vulneris OX=1977869 GN=CBF37_08675 PE=4 SV=1
MM1 pKa = 7.15AHH3 pKa = 6.09EE4 pKa = 5.17HH5 pKa = 5.82NHH7 pKa = 6.36DD8 pKa = 4.05HH9 pKa = 7.8DD10 pKa = 5.07HH11 pKa = 6.64DD12 pKa = 4.23QEE14 pKa = 4.38DD15 pKa = 5.49LITLIDD21 pKa = 3.98DD22 pKa = 4.09EE23 pKa = 4.86GNEE26 pKa = 3.99VLFRR30 pKa = 11.84IHH32 pKa = 6.3LTIDD36 pKa = 3.19GQEE39 pKa = 4.08QFGKK43 pKa = 10.41DD44 pKa = 3.49YY45 pKa = 11.45VLLYY49 pKa = 10.6DD50 pKa = 4.08ASTPEE55 pKa = 4.13GEE57 pKa = 4.42EE58 pKa = 4.23VEE60 pKa = 4.36LLAYY64 pKa = 10.01AYY66 pKa = 8.56EE67 pKa = 4.24QADD70 pKa = 3.52GDD72 pKa = 4.36AEE74 pKa = 4.3GQLMEE79 pKa = 4.7VEE81 pKa = 5.22TEE83 pKa = 4.29EE84 pKa = 4.12EE85 pKa = 3.96WNMIEE90 pKa = 4.11EE91 pKa = 4.29VFNAFEE97 pKa = 5.45DD98 pKa = 4.1EE99 pKa = 4.54DD100 pKa = 4.03SQEE103 pKa = 3.79

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A429ZTY0|A0A429ZTY0_9ENTE RNA-binding protein OS=Vagococcus vulneris OX=1977869 GN=CBF37_10220 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2196

0

2196

671661

28

2362

305.9

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.054

0.69 ± 0.015

5.685 ± 0.043

6.803 ± 0.06

4.564 ± 0.045

6.399 ± 0.053

1.821 ± 0.023

8.266 ± 0.053

7.411 ± 0.051

9.688 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.024

5.04 ± 0.048

3.28 ± 0.03

3.921 ± 0.04

3.686 ± 0.037

6.35 ± 0.049

5.848 ± 0.037

6.813 ± 0.043

0.856 ± 0.018

3.716 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski