Streptomyces alkaliterrae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5331 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P0YZB7|A0A5P0YZB7_9ACTN Amino acid permease OS=Streptomyces alkaliterrae OX=2213162 GN=FNX44_018565 PE=4 SV=1
MM1 pKa = 7.77PKK3 pKa = 10.26HH4 pKa = 6.48PIFVFDD10 pKa = 5.67PISAACDD17 pKa = 3.55LLSFGHH23 pKa = 7.28DD24 pKa = 3.28EE25 pKa = 4.75DD26 pKa = 5.74FVAAYY31 pKa = 10.09DD32 pKa = 3.91WIFSGDD38 pKa = 3.53QEE40 pKa = 4.23QGSFQQVCVDD50 pKa = 3.32SGYY53 pKa = 11.2NPLALQEE60 pKa = 4.54SIRR63 pKa = 11.84CDD65 pKa = 3.38FRR67 pKa = 11.84MLDD70 pKa = 2.94RR71 pKa = 11.84RR72 pKa = 11.84YY73 pKa = 10.47RR74 pKa = 11.84EE75 pKa = 3.74IVTAGDD81 pKa = 4.11GIDD84 pKa = 3.33AQEE87 pKa = 4.13FQMCDD92 pKa = 3.23EE93 pKa = 5.09DD94 pKa = 3.86TGEE97 pKa = 3.97WPLL100 pKa = 3.96

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P0YSY4|A0A5P0YSY4_9ACTN Uncharacterized protein OS=Streptomyces alkaliterrae OX=2213162 GN=FNX44_016410 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5331

0

5331

1683650

28

3258

315.8

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.522 ± 0.055

0.82 ± 0.011

6.011 ± 0.027

6.141 ± 0.033

2.639 ± 0.022

9.478 ± 0.031

2.299 ± 0.019

2.791 ± 0.024

2.025 ± 0.03

10.52 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.013

1.734 ± 0.019

6.175 ± 0.032

2.596 ± 0.018

8.75 ± 0.037

4.881 ± 0.024

5.871 ± 0.028

8.585 ± 0.033

1.482 ± 0.015

1.997 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski