Burkholderia phage KS10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5TA67|B5TA67_9CAUD Gp03 OS=Burkholderia phage KS10 OX=557289 PE=4 SV=1
MM1 pKa = 7.02STLTAEE7 pKa = 4.21QHH9 pKa = 6.03DD10 pKa = 4.54LRR12 pKa = 11.84DD13 pKa = 4.07KK14 pKa = 10.94IVALSAALNTPTSSPLKK31 pKa = 9.89LASEE35 pKa = 4.23IMAFGACAHH44 pKa = 6.85RR45 pKa = 11.84ASDD48 pKa = 3.71TDD50 pKa = 3.51MPIPDD55 pKa = 4.42EE56 pKa = 4.19VLLALDD62 pKa = 4.23DD63 pKa = 4.26DD64 pKa = 4.52LRR66 pKa = 11.84RR67 pKa = 11.84LALSGIVLVLLGTLQQEE84 pKa = 4.99GAA86 pKa = 3.9

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5TA83|B5TA83_9CAUD Gp19 OS=Burkholderia phage KS10 OX=557289 PE=4 SV=1
MM1 pKa = 7.45TNQTDD6 pKa = 3.19SHH8 pKa = 5.09ITIRR12 pKa = 11.84HH13 pKa = 4.59TAGRR17 pKa = 11.84TTVRR21 pKa = 11.84ATGRR25 pKa = 11.84YY26 pKa = 8.44ARR28 pKa = 11.84PFLDD32 pKa = 3.68ALANGAPSGEE42 pKa = 3.95AAAFSAWLTFQPRR55 pKa = 11.84TASEE59 pKa = 4.19VTAA62 pKa = 4.63

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11996

62

811

244.8

26.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.454 ± 0.547

0.992 ± 0.124

6.611 ± 0.233

5.502 ± 0.254

2.959 ± 0.183

7.761 ± 0.406

1.692 ± 0.183

4.301 ± 0.189

3.66 ± 0.294

8.586 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.191

3.318 ± 0.18

4.61 ± 0.194

4.218 ± 0.178

7.361 ± 0.27

5.819 ± 0.259

5.769 ± 0.258

7.611 ± 0.276

1.609 ± 0.155

2.459 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski